High spatial resolution diffuse optical tomography based on cross-correlation of chaotic light.

A diffuse optical tomography system with chaotic laser is proposed for the three-dimensional optical phantom. The high signal-to-noise ratio is beneficial to improve the spatial resolution of diffuse optical tomography. It is essential to drive the chaotic laser as the incident light into the optical phantom. The transmitted light emitted from phantom as the detection light and a part of the incident light as the reference light to carry out cross-correlation analysis. The high-density source-detector configuration in parallel plate structure is designed for detecting targets in the phantom. The propagation of chaotic laser in the phantom is studied theoretically and experimentally based on the diffusion equation. Image reconstruction is achieved by the cross-correlation analysis of chaotic laser and the Newton-Raphson nonlinear algorithm. The performance of the proposed system has been assessed by reconstruction localization accuracy and contrast-noise-ratio. The results show that the spatial resolution of the proposed system can reach 1.5 mm and the localization error is less than 1 mm.

Distinct enzyme activities of serine protease p37k in silkworm midgut and molting fluid.

Serine proteases possess various biological functions. The serine protease p37k exhibits gelatinolytic activity in the silkworm midgut and degrades cuticular proteins in the molting fluid. In this study, we analyzed the activity changes of recombinant p37k (re-p37k) and p37k in the midgut and molting fluid of Bombyx mori. Firstly, in vitro-expressed re-p37k was activated when a 22 kDa band was observed by western blot. Re-p37k exhibits strong gelatinolytic activity, with the highest activity observed at pH 7.0-9.0 and 45 °C. Compared to p37k in the midgut, re-p37k loses thermal stability but can be restored by midgut extract or ions. E64, AEBSF, and an inhibitor cocktail inhibited the hydrolytic activity of re-p37k on epidermal proteins but did not inhibit the gelatinolytic activity. Subsequently, zymography showed that the positions of gelatinolytic band produced by p37k in the midgut and molting fluid were different, 35 kDa and 40 kDa, respectively. Finally, when heated midgut extract was added to re-p37k or molting fluid, the gelatinolytic band shifted from 40 kDa to 35 kDa, and the proteolytic activity of p37k in the molting fluid was inhibited. Collectively, our results demonstrate that p37k exhibits different activities in various tissues, suggesting its distinct tissue-specific functions during insect metamorphosis.

Proteomic Analysis of the Midgut Contents of Silkworm in the Pupal Stage.

The silkworm Bombyx mori, a lepidopteran insect, possesses an 8-10-day pupal stage, during which significant changes occur in the midgut, where it first condenses into the yellow body, and then undergoes decomposition. To gain insights into this transformation process, proteomics was performed on Bombyx mori midgut contents on day 2 and day 7 after pupation. The results revealed the identification of 771 proteins with more than one unique peptide. An analysis using AgriGO demonstrated that these proteins were predominantly associated with catalytic activity. Among the identified proteins, a considerable number were found to be involved in carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleic acid degradation, and energy support. Additionally, variations in the levels of certain proteases were observed between the midgut contents on day 2 and day 7 after pupation. An in-depth analysis of the two-dimensional electrophoresis of the midgut contents on day 7 after pupation led to the identification of twelve protein spots with potential gelatinolytic activity. Among these, six proteases were identified through mass spectrometry, including the p37k protease, vitellin-degrading protease, chymotrypsin-2, etc. These proteases may be responsible for the digestion of the yellow body during the later stages of pupal development.

[Characterization and immunofluorescence localization analysis of carboxypeptidase A in molt fluid of silkworm].

Molting is an important physiological phenomenon of many metamorphosis insects, during which the old and new epidermis are separated by enzymes present in the molting fluid. Various proteomic studies have discovered the presence of Bombyx mori carboxypeptidase A (Bm-CPA) in the molting fluid of silkworm, but its function remains unclear. In order to better understand the role of Bm-CPA in the molting process of silkworm, Bm-CPA was analyzed by bioinformatics analysis, real-time fluorescence quantitative PCR, antibody preparation, immunofluorescence staining, and expression in Pichia pastoris. The results showed that Bm-CPA had a conserved M14 zinc carboxypeptidase domain and glycosylation site. Its expression was regulated by ecdysone 20E, and large expression was observed in the epidermis of the upper cluster stage. Immunofluorescence staining showed that Bm-CPA was enriched in the epidermis during the molting stage, and the inhibitor of Bm-CPA led to the larval death due to the inability to molt. We also successfully obtained a large number of recombinant Bm-CPA proteins by Pichia pastoris expression in vitro. These results may facilitate further understanding the molting development process of silkworm.

Formation of a D-shaped optical fiber by in-cavity online polishing for high resolution sensing in a chaotic correlation fiber loop ring down system.

Online monitoring of the polishing process of a D-shaped optical fiber sensor is performed in this paper by means of a chaotic correlation fiber loop ring down (CCFLRD) system. The ring down time of the autocorrelation coefficient decreases with the increase in polishing loss caused by different mesh sizes. A comparison of the sensitivity and resolution of the CCFLRD system with different polishing losses in the length of a fiber loop cavity are carried out. Online polishing in the fiber loop cavity with a very short length is proposed and demonstrated using different polishing mesh numbers to increase the sensitivity and resolution of the system. A high sensitivity of 31.871n s -1 R I U -1 and resolution of 10-4 R I U for refractive index sensing in the range of 1.3347-1.3721 correspond to different concentrations of sodium chloride.

Strain induced magnetic hysteresis in MoS2 and WS2 monolayers with symmetric double sulfur vacancy defects.

It has been found that magnetism in two-dimensional (2D) transition metal dichalcogenides can be realized by properly introducing vacancies and applying strain. However, no work has clearly clarified the modulation of such 2D magnetism under a sweeping strain. Thus we were motivated in this work to investigate the mechanical and electronic properties of the monolayer MS2 (M = Mo, W) with symmetric S vacancy defects under sweeping strain. The results show that the local structure of the M atoms in MS2 around the defect undergoes a reversible phase transition from a triangular shape (Tri-3M) with short M-M bonds, to a circular one (Cir-6M-12S) with larger M-M bonds as the planar strain increases. The critical tensile strain for the transition from Tri-3M to Cir-6M-12S are 12.53% for MoS2 and 11.46% for WS2, while the critical compressive strain for the reversal from Cir-6M-12S to Tri-3M are -3.60% and -2.16%, respectively. In particular, we find that the magnetism can be continuously modulated and undergoes a hysteresis loop behavior under the sweeping strains, with the residual magnetism being 2 µB. Our work theoretically predicts the promising prospect for exploring low-dimensional semiconductor spintronic devices working without applying a magnetic field.

Generation of random soliton-like beams in a nonlinear fractional Schrödinger equation.

We investigate the generation of random soliton-like beams based on the Kuznetsov-Ma solitons in a nonlinear fractional Schrödinger equation (NLFSE). For Lévy index α = 1, the Kuznetsov-Ma solitons split into two nondiffracting beams during propagation in linear regime. According to the different input positions of the Kuznetsov-Ma solitons, the diffraction-free beams can be divided into three different types: bright-dark, dark-bright and bright-bright beams. In the nonlinear regime, the Kuznetsov-Ma solitons can be evolved into random soliton-like beams due to the collapse. The number of soliton-like beams is related to the nonlinear coefficient and the Lévy index. The bigger the nonlinear coefficient, the more beams generated. Moreover, the peak intensity of soliton-like beams presents a Gaussian distribution under the large nonlinear effect. In practice, the evolution of KM soliton can be realized by a plane wave with a Gaussian perturbation, which can be confirmed that they have the similar dynamics of propagation. In two dimensions, the plane wave with a Gaussian perturbation can be evolved into a bright-dark axisymmetric ring beam in the linear regime. Under the nonlinear modulation, the energy accumulates to the center and finally breaks apart into random beam filaments.

Trypsin-type serine protease p37k hydrolyzes CPAP3-type cuticle proteins in the molting fluid of the silkworm Bombyx mori.

Cuticular proteins analogous to peritrophin 3 (CPAP3)-type cuticle proteins constitute a family of proteins with three chitin-binding domains (CBDs) that play an important role in cuticle formation by associating with chitin. In our previous study, we identified CPAP3-type cuticle proteins in the silkworm genome, of which we characterized CPAP3-A2 (BmCBP1), a protein highly expressed in the epidermis. In this study, to elucidate the digestion mechanism of CPAP3-type cuticle proteins, we incubated CPAP3-A2 with molting fluid in vitro and found that its hydrolysis, which was inhibited by serine and cysteine protease inhibitors, produced two major bands with a molecular weight of approximately 22 kD and 11 kD. A trypsin-type serine protease, p37k, was presumed to be responsible for hydrolyzing CPAP3-A2 based on liquid chromatography-tandem mass spectrometry analysis of naturally purified molting fluid. To verify this, p37k was subsequently expressed in Sf9 cells using the Bac-to-Bac baculovirus expression system. In its active form, the recombinant protease could successfully hydrolyze CPAP3-A2. Finally, we analyzed the CPAP3-A2 molting fluid digestion site. When arginine 169 of CPAP3-A2 was mutated to alanine, a weaker hydrolysis of mutant CPAP3-A2 was observed compared to that of normal CPAP3-A2. Collectively, we identified a trypsin-type serine protease that is involved in the degradation of CPAP3-type cuticle proteins, including CPAP3-A2, suggesting that this protease plays an important role during molting in Bombyx mori. These findings provide the basis for further elucidation of the mechanisms underlying insect molting and metamorphosis.

Early Molecular Events during Onset of Diapause in Silkworm Eggs Revealed by Transcriptome Analysis.

Diapause is a form of dormancy, and Bombyx mori silkworm embryos are ideal models for studying diapause in insects. However, molecular events in eggs during the onset of diapause remain unclear. In this study, transcriptome analyses were performed on silkworm diapause eggs via RNA sequencing at 20 and 48 h after oviposition. A total of 6402 differentially expressed genes (DEGs) were detected in diapause eggs at 48 h versus that at 20 h after oviposition. Gene ontology enrichment analysis showed that DEGs in diapause eggs at 48 h versus that at 20 h after oviposition were involved in ribosome-related metabolism and hydrogen transport. Kyoto Encyclopedia of Genes and Genomes analysis revealed several significantly enriched biological pathways, namely the oxidative phosphorylation, Forkhead box protein O3 (FoxO) signaling, ribosome, endoplasmic reticular protein processing, and autophagy pathways. Fifteen DEGs from the FoxO signaling pathway were selected, and their expression profiles were consistent with the transcriptome results from real-time quantitative reverse transcription polymerase chain reaction. Our results can improve understanding of the diapause mechanism in silkworm eggs and identified key pathways for future studies.

Optical sensors using chaotic correlation fiber loop ring down.

We have proposed a novel optical sensor scheme based on chaotic correlation fiber loop ring down (CCFLRD). In contrast to the well-known FLRD spectroscopy, where pulsed laser is injected to fiber loop and ring down time is measured, the proposed CCFLRD uses a chaotic laser to drive a fiber loop and measures autocorrelation coefficient ring down time of chaotic laser. The fundamental difference enables us to avoid using long fiber loop as required in pulsed FLRD, and thus generates higher sensitivity. A strain sensor has been developed to validate the CCFLRD concept. Theoretical and experiment results demonstrate that the proposed method is able to enhance sensitivity by more than two orders of magnitude comparing to the existing FLRD method. We believe the proposed method could find great potential applications for chemical, medical, and physical sensing.

Direct generation of all-optical random numbers from optical pulse amplitude chaos.

We propose and theoretically demonstrate an all-optical method for directly generating all-optical random numbers from pulse amplitude chaos produced by a mode-locked fiber ring laser. Under an appropriate pump intensity, the mode-locked laser can experience a quasi-periodic route to chaos. Such a chaos consists of a stream of pulses with a fixed repetition frequency but random intensities. In this method, we do not require sampling procedure and external triggered clocks but directly quantize the chaotic pulses stream into random number sequence via an all-optical flip-flop. Moreover, our simulation results show that the pulse amplitude chaos has no periodicity and possesses a highly symmetric distribution of amplitude. Thus, in theory, the obtained random number sequence without post-processing has a high-quality randomness verified by industry-standard statistical tests.

All-fiber broadband supercontinuum source with high efficiency in a step-index high nonlinear silica fiber.

We demonstrate an all-fiber broadband supercontinuum (SC) source with high efficiency in a step-index high nonlinear silica fiber, which was pumped by a 1557 nm subpicosecond-pulse laser in the normal dispersion region. The broad SC spectrum covers the spectral range from 840 to 2390 nm, and the 10 dB bandwidth from 1120 nm to 2245 nm of the SC covers one octave, assuming the peaks near 1550 nm were filtered. The SC source system is constructed by all-fiber components, which can be fusion-spliced together directly with low loss, less than 0.1 dB. Thus the SC source has high energy transfer efficiency from the pump source. The maximum SC average power of 332 mW is obtained, including the peaks near 1550 nm. The spectral density for the 10 dB bandwidth is in the range from -17.3 to -7.3 dBm/nm.