Enhancing the Development and Growth of Infant Cerebral Palsy Rats Using Selective Spinal Manipulations.

Cerebral palsy (CP) is a refractory pediatric disease with a high prevalence, high disability rate, and difficult treatment. A variety of treatments are currently used for CP. The treatment involves drug and non-drug therapy. Traditional Chinese medicine external therapy is a very distinctive treatment method in non-drug therapy. As one of the external therapies of traditional Chinese medicine, massage is used in treating cerebral palsy and has good efficacy, small side effects, and strong operability. As a part of TCM external therapy, selective spinal manipulation can effectively promote the growth and development of infant rats with cerebral palsy.The operation was mainly divided into four steps: first, the rubbing method was applied to the spine and both sides of the spine for 1 min. The pressing and kneading method was applied to the spine for 5 min, and the muscles on both sides of the spine for 5 min. Second, pressing and kneading the sensitive local acupoints in the spine for 2 min were performed. Thirdly, the affected limb was treated by twisting method for 1 min. Fourth, the rubbing method was applied to a midline from the forehead to the back of the brain for 1 min. This study aimed to use selective spinal manipulation to treat infant rats with cerebral palsy. The weight, Rotarod test, Foot-fault score, and growth hormone of infant rats with cerebral palsy were detected to understand the effect of selective spinal manipulation on the growth and development of infant rats with cerebral palsy. The results showed that it can promote weight gain, improve balance ability and motor function, promote growth and development of infant cerebral palsy rats, promote growth hormone secretion, and increase the temperature of sensitive parts of the back.

Reconstructing spatial pattern of historical cropland in karst areas of Guizhou, Southwest China.

Karst regions are exceptionally responsive to global change with their harsh natural environment, fragile ecology, and acute human-land conflicts. The reconstruction of cropland spatial pattern in karst areas during the historical period is typical for studying human-land relations in karst areas and has important practical significance for climate study. The ecological environment changes at regional and global scales, primarily to provide essential data and a theoretical basis for studying the inverse evolution of rock desertification in karst areas. Guizhou province, a typical karst area, was selected as the research area in 1820. Based on the correction of historical population data and cropland data, a reconstruction model of cropland spatial pattern in karst areas during the historical period was constructed by selecting factors such as elevation, slope, soil types, organic matter content, climatic productivity potential, distance to river and distance from settlements to reconstruct the spatial pattern distribution of cropland in 1820 of Guizhou. The results show that the data on cropland recorded in Guizhou during the Qing dynasty is too low, mainly due to Yin-Ni and the policy of Tu-Di-Mian-Ke. In 1820, the total area of revised cropland in Guizhou was 1,851,792 hm2, with the highest proportion of 14.32% in Dading Fu and the lowest in Songtao Ting at 1.6%. Only 30% of the grid in Guizhou has a cropland distribution. It is mainly concentrated in the central part of Qianzhong District (Anshun and Guiyang Fu), the southern part of Qianbei District (Pingyue Fu and southern Zunyi Fu), the western part of Qiandongnan District, the central and eastern parts of the Qiandongbei District. The overall average reclamation rate of land in Guizhou is 10.93%, the highest reclamation intensity in Qianzhong District, with 8.5% of grids ≥ 50%, and the smallest in Qianxinan District, with only 1.65% of grids ≥ 50%. The analysis is validated by comparing the reconstruction model and the reconstruction results. It can be seen that the reconstruction model and research results of this paper can more objectively reflect the distribution of cropland in karst areas during the historical period, and the reconstruction model is suitable for karst areas with low productivity levels.

Pharmacokinetics of ropivacaine in elderly patients receiving fascia iliaca compartment block.

Local anesthetic of ropivacaine was demonstrated to reduce the postoperative pain in elderly patients. This study investigated the pharmacokinetics of ropivacaine at different concentrations in elderly patients subjected to fascia iliaca compartment block. Forty patients with femoral neck fracture at American Society of Anesthesiologists (ASA) I-II status, undergoing fascia iliaca compartment block (FICB) were randomized to two groups receiving 0.7 ml/kg of solution containing 0.375% ropivacaine (group L) or 0.5% ropivacaine (group H). Samples of venous blood were obtained immediately at different time points after FICB, and the total and free plasma concentrations of ropivacaine were measured by liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS). Statistical analysis was carried out using a pharmacokinetic calculation program (DAS 3.0). Visual Analogue Scale (VAS) scores were significantly decreased after FICB in both groups, and VAS score in group H was lower compared with group L. The total maximum plasma concentration (Cmax) and the free Cmax of ropivacaine in group H was higher than that in group L (P<0.05). The decrease of the total and free plasma concentration was operation time-dependent. Neither group showed signs of central nervous system and circulatory system toxicity. On the basis of these results, the concentrations of 0.375 and 0.5% ropivacaine held an efficiently analgesic effect for FICB, suggesting that ropivacaine can be employed in analgesic therapy. However, both concentrations have a potentially theoretical risk of local anesthetics poisoning, suggesting that a lower concentration may be a safer option for a single large volume of FICB.

Lin28b and Sox2 regulate anesthesia-induced neural degeneration in neural stem cell derived neurons.

Ketamine, a commonly used anesthetic compound, may cause neurotoxicity in immature or developing brains. In this study, we indented to understand the molecular expression and functional role of Lin-28 Homolog B (lin28b) in regulating ketamine-induced neurotoxicity in neural stem cells (NSCs)-differentiated neurons. NSCs from embryonic rat brains were cultured in vitro, and induced toward neuronal differentiation. NSCs-differentiated neurons were treated with various concentrations of ketamine for 24h to evaluate the concentration-dependent effect of ketamine on endogenous lin28b mRNA level. QRT-PCR showed that lin28b was downregulated by ketamine in NSCs-differentiated neurons, in concentration-dependent manner. Neurons were then transfected with adenovirus to ectopically upregulate lin28b. We found that ketamine-induced apoptosis and neurite retraction in NSCs-differentiated neurons were significantly reduced by adenovirus-mediated lin28b upregulation. Expression of sex determining region Y box 2 (Sox2) mRNA was examined in ketamine-injured and lin28b-upregulated NSCs-differentiated neurons. It was found Sox2 was downregulated by ketamine, and overexpressed by lin28b upregulation. Finally, Sox2 was downregulated by siRNA in NSCs-differentiated neurons. And we discovered that Lin28b-upregulation-associated neural protection was severely hampered by Sox2 downregulation in ketamine-injured neurons. Thus, Lin28b and Sox2 are important molecular components in ketamine-induced neurotoxicity.