ABSTRACT
As potential alternatives for calcium phosphate bone cements, magnesium phosphate bone cements (MPC) have attracted considerable attention in recent years. However, their several defects, such as rapid setting times, highly hydration temperature and alkaline pH due to the part of the unreacted phosphate, restricted their applications in human body. With aim to overcome these defects, a novel polypeptite poly(γ-glutamic acid) (γ-PGA) modified MPC were developed. Effect of γ-PGA content on the injectability, anti-washout ability, setting times, hydration temperature, mechanical compressive strength, in vitro bioactivity and degradation were investigated. Moreover, in vitro cyto-compatibility was evaluated using MC3T3-E1 cells by CCK-8 and Live/Dead staining. All these results indicated that the 10%PGA-MPC with an improved handling performances, low hydration temperature, high mechanical compressive strength, and good cyto-compatibility hold a great potential for bone repair and regeneration.
Subject(s)
Bone Cements , Magnesium Compounds , Phosphates , Polyglutamic Acid/analogs & derivatives , Humans , Bone Cements/pharmacology , Bone Cements/chemistry , Materials Testing , Phosphates/chemistry , Calcium Phosphates/chemistry , Bone Regeneration , Compressive StrengthABSTRACT
Methylated chromenoquinoline dyes were designed and synthesized, and their photophysical properties were investigated. These new fluorescent dyes exhibited relatively high quantum yields, long wavelength emissions, large Stokes shifts, large absorption extinction coefficients and good water-solubility. Importantly, these methylated cation dyes showed a specific staining ability for mitochondria in living cells with low cytotoxicity.
Subject(s)
Fluorescent Dyes/chemical synthesis , Mitochondria/metabolism , Quinolines/chemical synthesis , Staining and Labeling/methods , Cell Survival/drug effects , Dose-Response Relationship, Drug , Fluorescent Dyes/analysis , Fluorescent Dyes/chemistry , HeLa Cells , Humans , Methylation , Molecular Structure , Optical Phenomena , Quinolines/analysis , Quinolines/chemistry , Solubility , Structure-Activity Relationship , WaterABSTRACT
4-(Benzothiazol-2-yl)-3-hydroxybenzaldehyde, probe 1, has been developed as a ratiometric fluorescent probe for the sensitive and selective detection of sulfur dioxide with a fast response time (within seconds). This probe itself exhibited a yellow emission (λmaxem = 563 nm) with a 186 nm Stokes shift. Upon treatment with sulfite anions, this probe instantaneously displayed a blue emission (λmaxem = 467 nm) with a 133 nm Stokes shift. The large Stokes shifts and absorption spectral overlap of probe 1 both in the absence and in the presence of SO3(2-)/HSO3(-) allow the fluorescence detection under single wavelength excitation. Importantly, this ratiometric probe was successfully demonstrated for the imaging of intracellular sulfite anions in living cells.
