ABSTRACT
The genus Unio is one of the widespread freshwater bivalves. To date, its intra-generic phylogeny remains controversial and therefore the mitochondrial genome data is needed. Here, we report the complete mitogenome of Unio douglasiae MG that is distributed in the Chihe River, a branch of Huaihe River, East China. This mitochondrial genome is 15,764 base pair in total length. It consists of 37 genes: 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes (12S and 16S). The base composition was 38.38% for A, 26.48% for T, 23.17% for C, and 11.98% for G, showing an obvious bias of higher A + T content (64.86%) than the G + C content (35.14%). Phylogenetic analysis showed that U. douglasiae MG is clustered with other Unio and Nodularia mollusks in the family Unionidae. These results showed that combine with morphological techniques, the mitogenome can provide useful information to further understanding of the genetics, systematics, and conservation of this endangered species.
ABSTRACT
Sarcocheilichthys sinensis (Bleeker), is a small-sized benthopelagic fish with ornamental value. In the present study, the complete mitochondrial genome of S. sinensis was sequenced and determined. The complete mitogenome of S. sinensis was 16,683 bp in length, consisting of 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 2 non-coding regions. The overall base composition of the S. sinensis mitogenome is 30.50% A, 26.28% T, 26.60% C, and 16.61% G, exhibiting obvious AT bias (56.79%). The phylogenetic analysis showed that S. sinensis clustered in genus Sarcocheilichthys. Present study provides useful data to population genetics and conservation biology of Sarcocheilichthys fishes.
ABSTRACT
In the present study, the complete mitogenome sequence of a Acanthorhodeus chankaensis Dybowsky from Cao'e River was sequenced and identified. The assembled mitogenome of A. chankaensis is 16,676 bp in length, it contains 22 transfer-RNA genes, 13 protein-coding genes, 2 ribosomal-RNA genes, and 2 non-coding regions. It shows conserved gene arrangement with other Cyprinidae fishes. The overall nucleotide composition of A. chankaensis mitogenome sequence is A: 28.96%, G: 17.11%, T: 27.46%, and C: 26.47%. The phylogenetic analysis showed that the complete mitogenome could contribute to the phylogenetic analyses and population genetics study of A. chankaensis and Acanthorhodeus fish.
ABSTRACT
Northern snakehead, Channa argus, is a commercially important food fish species in China. In the present study, the complete mitochondrial genome of C.argus from the Baima Hu Lake was characterized. It is 16,558 bp in length, consist of 22 tRNA genes, 13 PCD genes, 2 rRNA genes, and 1 D-loop region. The overall base composition of the C. argus mitogenome is 27.26% A, 24.21% T, 31.58% C and 16.95% G, exhibits a similar AT bias (51.47%) feature to other vertebrate mitogenomes. The phylogenetic analysis showed that C. argus clustered in genus Channa. The present resultes provide useful information to population genetics and conservation biology studies of Channa fishes.
ABSTRACT
The complete mitochondrial genome of Hemibarbus labeo BML (Cypriniformes: Cyprinidae) is 16,612 bases in length. It consists of 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 1 non-coding region. Its overall nucleotide composition is A: 29.85%, G: 17.07%, T: 25.86%, and C: 27.23%, respectively, with an A + T rich feature (55.71%). The gene arrangement and organization of the mitogenome from H. labeo BML were very similar to other Cyprinidae fishes. The phylogenetic analysis showed that H. labeo BML clustered in genus Hemibarbus. These results will contribute to the taxonomy and conservation biology studies of H. labeo.
ABSTRACT
Solenaia oleivora, belongs to Bivalvia, Unionidae, and Gonideinae, is a burrowing bivalve uniquely distributed in China. In this study, the complete mitochondrial genome of S. oleivora MG was sequenced and determined. The complete mitogenome of S. oleivora MG is 16,392 bp in total length, consist of 22 tRNA genes, 13 protein-coding genes (PCGs), and 2 rRNA genes. The overall base composition of the S. oleivora MG mitogenome is 36.90% A, 23.85% T, 27.09% C, and 12.16% G, respectively, exhibits a similar AT bias (60.75%) feature to other invertebrate bivalve mitogenomes. The phylogenetic analysis that S. oleivora MG clustered in genus Solenaia. This result provides useful data to the conservation and sustainable utilization of S. oleivora MG and other invertebrate mussels.
ABSTRACT
In this study, the complete mitochondrial genome sequence of a Xenocypris davidi from Cao'e River was sequenced. The complete mitogenome of X. davidi was 16,630 bp in length, it contains the structure of 22 transfer RNA genes, 13 protein coding genes, 2 ribosomal RNA genes, and 1 non-coding region. The gene arrangement and organization in the mitogenome of X. davidi were in accordance with other Cyprinidae fishes. The results of phylogenetic analysis revealed that the mitochondrial genome sequence could provide useful information for the conservation genetics and evolution study of X. davidi.
ABSTRACT
Cyclophilins (CyPs) are a family of proteins that bind the immunosuppressive agent cyclosporin A (CsA) with high-affinity and belong to one of the three superfamilies of peptidyl-prolyl cis-trans isomerases (PPIase). In this report, three cyclophilin genes (Ca-CyPs), including Ca-CyPA, Ca-CyPB and Ca-PPIL3, were identified from oyster, Crassostrea ariakensis Gould in which Ca-CyPA encodes a protein with 165 amino acid sequences, Ca-CyPB encodes a protein with 217 amino acid sequences and Ca-PPIL3 encodes a protein with 162 amino acid sequences. All of the three Ca-CyPs genes contain a typical CyP-PPIase domain with its signature sequences and Ca-CyPB contains an N-signal peptide sequences. Tissue distribution study revealed that Ca-CyPs were ubiquitously expressed in all examined tissues and the highest levels were observed in hemocytes. RLO incubation upregulated the mRNA expression levels of Ca-CyPs, indicating that three Ca-CyPs might be involved in oyster immune response against RLO infection.
Subject(s)
Crassostrea/genetics , Crassostrea/immunology , Cyclophilins/genetics , Gene Expression Regulation , Immunity, Innate , Amino Acid Sequence , Animals , Cloning, Molecular , Cyclophilins/chemistry , Cyclophilins/metabolism , DNA, Complementary/genetics , DNA, Complementary/metabolism , Protein Sorting Signals , RNA, Messenger/genetics , RNA, Messenger/metabolism , Sequence Alignment/veterinaryABSTRACT
In the present study, the complete mitogenome sequence of a Sarcocheilichthys parvus Nichols was sequenced and identified. It contained 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes and 2 non-coding regions. The phylogenetic analysis showed that the mitogenome sequence could be useful data in the field of systematics and conservation biology for S. parvus Nichols and other aquatic species.
Subject(s)
Cyprinidae/genetics , Genome, Mitochondrial , Mitochondria/genetics , Sequence Analysis, DNA/methods , Animals , Base Composition , China , Genes, rRNA , Genome Size , Phylogeny , RNA, Transfer/geneticsABSTRACT
In the present study, the total mitochondrial genome of a precocious Chinese mitten crab Eriocheir sinensis was firstly determined. The genome is 16,350 bp in length. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a control region. The overall nucleotide composition of the mitochondrial genome is A: 35.31 %, G: 10.69 %, T: 36.37 % and C: 17.63%, respectively. These results provide useful information to species identification, conservation and analysis the mechanisms of precocity in E. sinensis.
Subject(s)
Brachyura/genetics , Genome, Mitochondrial , Animals , Arthropod Proteins/chemistry , Arthropod Proteins/genetics , Base Composition , Codon, Initiator , Codon, Terminator , DNA, Mitochondrial/chemistry , DNA, Mitochondrial/isolation & purification , DNA, Mitochondrial/metabolism , Open Reading Frames/genetics , RNA, Ribosomal/chemistry , RNA, Ribosomal/genetics , RNA, Transfer/chemistry , RNA, Transfer/genetics , Sequence Analysis, DNAABSTRACT
Hyriopsis cumingii is a freshwater pearl mussel in china, easily identified by a big sail-like bulging at back edge of the mussel. In the present study, we reported the complete mitochondrial genome of H. cumingii. It was 16,958 bp in length, and it contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes. Besides, we conducted alignment with other two different individuals of H. cumingii and phylogenetic analysis with closely related species.
Subject(s)
Genome, Mitochondrial/genetics , Unionidae/genetics , Animals , DNA, Complementary , Phylogeny , RNA, Ribosomal/genetics , RNA, Transfer/genetics , Unionidae/classificationABSTRACT
Hemibarbus is a genus of cyprinid fishes distributed in eastern Asia. In the present study, we report here the complete mitochondrial genome of the Hemibarbus sp.090914 (Cypriniformes: Cyprinidae). Our results show that the complete mitochondrial DNA of Hemibarbus sp.090914 is 16,610 bp in length, and predicted to encode all the 37 genes that are typical for the vertebrates.
Subject(s)
Cyprinidae/genetics , Genome, Mitochondrial , Animals , Base Composition/genetics , Base Pairing/genetics , DNA, Mitochondrial/genetics , Genes, Mitochondrial , RNA, Ribosomal/genetics , RNA, Transfer/geneticsABSTRACT
In the present study, the total mitochondrial genome of a freshwater mussel Cristaria plicata (Leach) was sequenced and determined. The mitogenome is 15,710 bp in length. It consists of 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes. The total base composition is: 36.49% A, 27.23% T, 23.21% C, and 13.07% G with an A+T rich feature (63.72%). These results provide useful data to study of the genetics, systematics and conservation of mussel C. plicata (Leach).
Subject(s)
Genome, Mitochondrial , Mitochondria/genetics , Unionidae/genetics , Animals , Base Composition , Genome Size , Sequence Analysis, DNA/methodsABSTRACT
Pseudobagrus ondon is a small benthopelagic freshwater species of family Bagridae. In the present study, the complete mitochondrial genome of P. Ondon has been determined, and phylogenetic analysis with another individual and closely related species are preformed. The complete mitochondrial genome of P. ondon was 16,534 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 2 non-coding regions: a control region (D-loop) and an L-strand replication origin (OL). It indicated that D-loop, ND1, ND2, ND4, ND5 and CYTB may be appropriate markers for studying population as well as conservation genetics.
Subject(s)
Catfishes/genetics , Genome, Mitochondrial/genetics , Sequence Analysis, DNA , Animals , Genes, rRNA/genetics , Molecular Sequence Annotation , Molecular Sequence Data , Open Reading Frames/genetics , Phylogeny , RNA, Transfer/geneticsABSTRACT
Sarcocheilichthys sinensis sinensis (Bleeker, 1871), is a small benthopelagic freshwater species with high nutritional and ornamental value. In this study, the complete mitochondrial genome of S. sinensis sinensis was determined; the phylogenetic analysis with another individual and closely related species of Sarcocheilichthys fishes was carried out. The complete mitogenome of S. sinensis sinensis was 16683 bp in length, consist of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 2 non-coding regions: (D-loop and OL). It indicated that D-loop, ND2, and CytB may be appropriate molecular markers for studying population genetics and conservation biology of Sarcocheilichthys fishes.
Subject(s)
Cyprinidae/genetics , Genome, Mitochondrial/physiology , Phylogeny , Animals , Base Sequence , Fish Proteins/genetics , Mitochondrial Proteins/genetics , Molecular Sequence Data , RNA/genetics , RNA, Mitochondrial , RNA, Ribosomal/genetics , RNA, Transfer/geneticsABSTRACT
In this paper, we sequenced the complete mitochondrial genome of the Squalidus sp.SX0527 (Cypriniformes: Cyprinidae). The genome is 16,608 bp in size; it contains 22 tRNA genes, 13 PCD genes, 2 rRNA genes, and 2 non-coding regions. The total base composition is: 30.00% A, 25.48% T, 27.67% C, and 16.85% G, respectively. This study will contribute to species identification and natural resources conservation of Squalidus fishes.
Subject(s)
Genome, Mitochondrial/physiology , Perciformes/genetics , Phylogeny , Animals , Base Sequence , Fish Proteins/genetics , Mitochondrial Proteins/genetics , Molecular Sequence Data , RNA/genetics , RNA, Mitochondrial , RNA, Ribosomal/genetics , RNA, Transfer/geneticsABSTRACT
In the present study, the complete mitochondrial genome of the Sarcocheilichthys nigripinnis (Cypriniformes: Cyprinidae) was sequenced and identified, the phylogenetic analysis with other individual of Sarcocheilichthys fishes was carried out. Totally, this genome is 16,679 bp in length, which consists of 13 PCDs, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions. The total base composition is: 30.05% A, 26.40% T, 26.70% C, and 16.85% G, respectively. These results will provide useful data for species identification and genetics study of Sarcocheilichthys fishes.
Subject(s)
Cyprinidae/genetics , Genome, Mitochondrial/physiology , Animals , Base Sequence , Fish Proteins/genetics , Mitochondrial Proteins/genetics , Molecular Sequence Data , RNA/genetics , RNA, Mitochondrial , RNA, Ribosomal/genetics , RNA, Transfer/geneticsABSTRACT
In the present study, the total mitochondrial genome of Microphysogobio tafangensis (Cypriniformes: Cyprinidae) was determined. The full genome was 16,683 bp in length, which consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 2 non-coding regions. The nucleotide composition of M. tafangensis mitochondrial genome was A: 29.38%, G: 17.71%, T: 25. 47% and C: 27.44%.
Subject(s)
Cyprinidae/genetics , Genome, Mitochondrial , Animals , Base Composition/genetics , Base Sequence , Codon/genetics , DNA, Mitochondrial/genetics , Open Reading Frames/genetics , RNA, Transfer/geneticsABSTRACT
The Chinese mitten crab Eriocheir sinensi belongs to the family Decapoda, Varunidae, Eriocheir. In this study, the full length of mitochondrial genome of an ovulated Chinese mitten crab was first sequenced and analyzed. It was determined to be 16,353 bp in length, and consists of 13 protein coding genes (PCDs), 22 tRNA genes, 2 rRNA genes and 1 control region.
Subject(s)
Crustacea/genetics , Genome, Mitochondrial , Animals , Female , Male , Proteins/genetics , RNA, Ribosomal/genetics , RNA, Transfer/geneticsABSTRACT
In this study, the complete mitochondrial genome of Abbottina rivularis was determined; the phylogenetic analysis with other individuals and closely related species of the gudgeons was carried out. The complete mitogenome of A. rivularis was 16,597 bp in length, which consists of 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 2 non-coding regions: (D-loop and OL). The overall nucleotide composition of the A. rivularis mitogenome was A: 29.92%, T: 25.75%, G: 17.15% and C: 27.18%, respectively, with an A + T rich feature (57.1%). This study provides useful data to genetics, conservation and evolution study of the gudgeons.
