Directed Accumulation of Nitrogen Metabolites through Processing Endows Wuyi Rock Tea with Singular Qualities.

The execution of specific processing protocols endows Wuyi rock tea with distinctive qualities produced through signature metabolic processes. In this work, tea leaves were collected before and after each of three processing stages for both targeted and untargeted metabolomic analysis. Metabolic profiles of processing stages through each processing stage of rotation, pan-firing and roasting were studied. Overall, 614 metabolites were significantly altered, predominantly through nitrogen- enriching (N) pathways. Roasting led to the enrichment of 342 N metabolites, including 34 lipids, 17 organic acids, 32 alkaloids and 25 amino acids, as well as secondary derivatives beneficial for tea quality. This distinctive shift towards enrichment of N metabolites strongly supports concluding that this directed accumulation of N metabolites is how each of the three processing stages endows Wuyi rock tea with singular quality.

Left-behind experience and language proficiency predict narrative abilities in the home language of Kam-speaking minority children in China.

Introduction: Studies have documented that child experiences such as external/environmental factors as well as internal factors jointly affect acquisition outcomes in child language. Thus far, the findings have been heavily skewed toward Indo-European languages and children in the Western, educated, industrialized, rich and democratic (WEIRD) societies. By contrast, this study features an understudied minority language Kam, and a group of so-called left-behind children in China growing up in a unique social-communicative environment. Methods: Fifty-five bilingual children aged 5-9 acquiring Kam as home language were assessed using the Multilingual Assessment Instrument for Narratives (LITMUS MAIN). Twenty-three "two parents-left" children (mean age = 6;8, range: 5;0-9;2) remained in rural areas while both parents went to cities for employment, and they were raised by their grandparents. Thirty-two were "one parent-left" peers (mean age = 7;3, range: 5;0-9;3) who also resided in rural areas but were raised by one parent. Oral narrative texts were analysed for macrostructure based on story structure (SS), story complexity (SC) and internal state terms (IS). The study examined whether and how narrative production is predicted by internal factors such as chronological age and linguistic proficiency of a child and an external factor such as left-behind experience. Four measures were scored as outcome measures: SS, SC, IS type, IS token. Four measures were taken as predictors: chronological age, left-behind experience, scores in a lexical production task, and scores in a sentence repetition task tapping expressive morphosyntactic competence. Results: Results showed that left-behind experience consistently predicted all four outcome measures, where the "two parents-left" children scored significantly lower than their "one parent-left" peers. Expressive vocabulary scores predicted three measures: SS, SC, and IS Token. Expressive morphosyntactic scores predicted SS and SC. Age, by contrast, did not predict any outcome measure. Discussion: These findings suggested that being left-behind by both parents may be a negative prognostic indicator for the development and maintenance of heritage language abilities in ethnic minority children. We further discussed the conceptual significance of what it means for a child to be left-behind, by relating to more basic external factors in language development, including caregiver educational level, and amount of home language and literacy support by the caretakers.

Four-year-old Mandarin-speaking children's online comprehension of relative clauses.

A core question in language acquisition is whether children's syntactic processing is experience-dependent and language-specific, or whether it is governed by abstract, universal syntactic machinery. We address this question by presenting corpus and on-line processing dat a from children learning Mandarin Chinese, a language that has been important in debates about the universality of parsing processes. The corpus data revealed that two different relative clause constructions in Mandarin are differentially used to modify syntactic subjects and objects. In the experiment, 4-year-old children's eye-movements were recorded as they listened to the two RC construction types (e.g., Can you pick up the pig that pushed the sheep?). A permutation analysis showed that children's ease of comprehension was closely aligned with the distributional frequencies, suggesting syntactic processing preferences are shaped by the input experience of these constructions.

Four-year-old Cantonese-speaking children's online processing of relative clauses: a permutation analysis.

We report on an eye-tracking study that investigated four-year-old Cantonese-speaking children's online processing of subject and object relative clauses (RCs). Children's eye-movements were recorded as they listened to RC structures identifying a unique referent (e.g. "Can you pick up the horse that pushed the pig?"). Two RC types, classifier (CL) and ge3 RCs, were tested in a between-participants design. The two RC types differ in their syntactic analyses and frequency of occurrence, providing an important point of comparison for theories of RC acquisition and processing. A permutation analysis showed that the two structures were processed differently: CL RCs showed a significant object-over-subject advantage, whereas ge3 RCs showed the opposite effect. This study shows that children can have different preferences even for two very similar RC structures within the same language, suggesting that syntactic processing preferences are shaped by the unique features of particular constructions both within and across different linguistic typologies.

Expression of the Clonostachys rosea lactonohydrolase gene by Lactobacillus reuteri to increase its zearalenone-removing ability.

BACKGROUND: Mycotoxins are secondary metabolites produced by filamentous fungi that can contaminate agricultural crops in the field as well as during harvest, transportation, processing, or storage. Zearalenone (ZEN), a non-steroidal estrogenic mycotoxin, produced by Fusarium species, has been shown to be associated with reproductive disorders in farm animals and to a lesser extent in hyperoestrogenic syndromes in humans. Thus, the decontamination of ZEN in foods and feeds is an important issue. RESULTS: In this study, the gene encoding ZHD101, a ZEN-degrading enzyme produced by Clonostachys rosea IFO 7063, was cloned into an Escherichia coli-Lactobacillus shuttle vector, pNZ3004, and the resultant plasmid pNZ-zhd101 was then introduced via electroporation into Lactobacillus reuteri Pg4, a probiotic strain isolated from the gastrointestinal tract of broilers. The transformed strain L. reuteri pNZ-zhd101 acquired the capacity to degrade ZEN. In addition, the production of recombinant ZHD101 did not affect cell growth, acid and bile salt tolerance, and had only a minor effect on the adhesion ability of L. reuteri pNZ-zhd101. CONCLUSIONS: To the best of our knowledge, this is the first report of successful expression of a ZEN-degrading enzyme by intestinal lactobacilli.