Effects of coal gangue on soil property and plant growth in mining area.

With indoor and outdoor treatments, coal gangue with different ratios (10%, 20%, 30%, 40% and 50%) and different particle sizes (0-2, 2-5, 5-8 and 8-10 mm) were mixed into soil, and formed reconstructed soil with different soil bulk densities (1.3, 1.35, 1.4, 1.45 and 1.5 g·cm-3). The effects of soil reconstruction modes on soil water condition, structure stability of aggregate and the growth of Lolium perenne, Medicago sativa, and Trifolium repens were investigated. Soil saturated water (SW), capillary water (CW), and field water capacity (FC) were decreased with increasing coal gangue ratio, particle size, and bulk density of reconstructed soil. >0.25 mm particle size aggregate (R0.25), mean weight diameter (MWD), and geometric mean diameter (GMD) increased first and then decreased with the increases of coal gangue particle size, reaching the peak at 2-5 mm coal gangue particle size. R0.25, MWD and GMD were significantly and negatively correlated with coal gangue ratio. Based on boosted regression tree (BRT) model, the coal gangue ratio contributed 59.3%, 67.0% and 40.3% to the variation of SW, CW and FC, respectively, which was a main influencing factor of soil water content. The coal gangue particle size contributed 44.7%, 32.3% and 62.1% to the variation of R0.25, MWD, and GMD, respecti-vely, which was the greatest influencing factor. Coal gangue ratio had a great effect on the growth of L. perenne, M. sativa, and T. repens, which contributed to 49.9%, 17.4% and 10.3% of their variations, respectively. Soil reconstruction mode of 30% coal gangue ratio and 5-8 mm coal gangue particle size was the best condition for plant growth, indicating that coal gangue could change soil water content and structure stability of aggregate. The soil reconstruction mode of 30% coal gangue ratio and 5-8 mm coal gangue particle size was recommended.

Genetic diversity and molecular epidemiology of Candida albicans from vulvovaginal candidiasis patients.

Candida albicans (C. albicans) is a common cause of vulvovaginal candidiasis (VVC). In this paper, the genetic diversity and molecular epidemiology of 173C. albicans strains were investigated by multilocus sequence typing (MLST). A total of 52 diploid sequence types (DSTs) were recognized, and 27 (51.9%) of which have not been reported in the MLST database. Genotyping was performed on the multiple isolates collected from patients with recurrent VVC (RVVC, referring to VVC which attacks more than 4 times in one year) in different acute infectious phases. The results showed that 59.1% (26/44) of the patients suffered a relapse, with DST 79 (65.4%) as the dominant genotype. The etiology of the remaining 40.9% (18/44) of patients was reinfection, and the main genotypes included DST 79 (33.3%), DST 124 (8.6%) and DST 1895 (8.6%). DST 79 (45%) and DST 1395 (7.5%) were the main isolates of VVC patients, while DST 79 (24.1%), DST 727 (6.9%), DST 732 (6.9%) and DST 1867 (6.9%) were the main types of healthy volunteers. The results of the genotypes between RVVC patients and other groups were statistically different. Furthermore, cluster analysis was carried out on 1468 isolates, among which 1337 were downloaded from the MLST database, 130 were divided into 8 Clades in the present study and the remaining one was taken as a singleton. 92.3% isolates from relapse patients, 58.3% isolates from re-infected patients, 77.5% isolates from VVC patients and 51.7% isolates from volunteers were distributed in Clade 1. The analysis of the genotypes of multiple isolates from RVVC patients further demonstrated that point mutation and loss of heterozygosity contributed to the microevolution of C. albicans.

Correction to: Duganella rivus sp. nov., Duganella fentianensis sp. nov., Duganella qianjiadongensis sp. nov. and Massilia guangdongensis sp. nov., isolated from subtropical streams in China and reclassification of all species within genus Pseudoduganella.

In the published version of the article, the title should have read 'Duganella rivi sp. nov., Duganella fentianensis sp. nov., Duganella qianjiadongensis sp. nov. and Massilia guangdongensis sp. nov., isolated from subtropical streams in China and reclassification of all species within genus Pseudoduganella'.

Duganella rivus sp. nov., Duganella fentianensis sp. nov., Duganella qianjiadongensis sp. nov. and Massilia guangdongensis sp. nov., isolated from subtropical streams in China and reclassification of all species within genus Pseudoduganella.

Four Gram-stain-negative, catalase-positive, rod-shaped and motile strains (FT55WT, FT93WT, CY13WT and DS3T) were isolated from subtropical streams in China. Comparisons based on 16S rRNA gene sequences indicated that strains FT55WT, FT93WT and CY13WT take strain Pseudoduganella danionis E3/2T, and strain DS3T takes strain Pseudoduganella eburnea 10R5-21T as their closest neighbour, respectively. The genome sizes of strains FT55WT, FT93WT, CY13WT and DS3T were 6.15, 5.10, 5.31 and 5.72 Mbp with G+C contents of 61.7, 60.9, 60.6 and 64.0%, respectively. The reconstructed phylogenomic tree based on concatenated 92 core genes showed that strain FT55WT clusters closely with Duganella radicis KCTC 22382T and Duganella sacchari Sac-22T, strains FT93WT and CY13WT form a distinct clade with P. danionis DSM 103461T and this clade clusters with the clades of genus Duganella together, and strain DS3T forms a distinct clade with P. eburnea JCM 31587T and Pseudoduganella violaceinigra DSM 15887T and this clade clusters closely with the clades of genus Massilia, respectively. The calculated pairwise OrthoANIu values and digital DNA-DNA hybridization (DDH) values among strains FT55WT, FT93WT, CY13WT, DS3T and related strains were in the ranges of 75.6-94.2% and 20.6-56.2%, respectively. Q-8 was the sole respiratory quinone of these four strains. The major fatty acids were C16:1ω7c, C16:0 and C12:0. The polar lipids included phosphatidylglycerol, phosphatidylethanolamine and one unidentified phospholipid. Considering the similar fatty acids and polar lipids profiles of species within genus Pseudoduganella, Massilia and Duganella, there is currently no justification for assigning the species of genus Pseudoduganella into the Massilia and Duganella clades in the phylogenomic tree. It is reasonable to transfer P. violaceinigra and P. eburnea to the genus Massilia as Massilia violaceinigrum comb. nov. and Massilia eburnea comb. nov., and transfer P. danionis to the genus Duganella as Duganella danionis comb. nov. Considering phylogenomic analysis, OrthoANIu data, digital DDH data and a range of physiological and biochemical characteristics, strains FT55WT, FT93WT and CY13WT should be assigned to genus Duganella, and strain DS3T should be classified as a novel species within genus Massilia, for which the names Duganella rivus sp. nov. (type strain FT55WT = GDMCC 1.1675T = KACC 21467T), Duganella fentianensis sp. nov. (type strain FT93WT = GDMCC 1.1683T = KACC 21475T), Duganella qianjiadongensis sp. nov. (type strain CY13WT = GDMCC 1.1669T = KACC 21461T) and Massilia guangdongensis sp. nov. (type strain DS3T = GDMCC 1.1636T = KACC 21312T) are proposed.

[Hydrologic processes of the different landscape zones in Fenhe River headwater catchment].

There are few studies on the hydrologic processes of the landscape zone scales at present. Since the water environment is worsening, there is sharp contradiction between supply and demand of water resources in Shanxi province. The principle of the hydrologic processes of the landscape zones in Fenhe River headwater catchment was revealed by means of isotope tracing, hydrology geological exploration and water chemical signal study. The results showed that the subalpine meadow zone and the medium high mountain forest zone were main runoff formation regions in Fenhe River headwater catchment, while the sparse forest shrub zone and the mountain grassland zone lagged the temporal and spatial collection of the precipitation. Fenhe River water was mainly recharged by precipitation, groundwater, melt water of snow and frozen soil. This study suggested that the whole catchment precipitation hardly directly generated surface runoff, but was mostly transformed into groundwater or interflow, and finally concentrated into river channel, completed the "recharge-runoff-discharge" hydrologic processes. This study can provide scientific basis and reference for the containment of water environment deterioration, and is expected to deliver the comprehensive restoration of clear-water reflowing and the ecological environment in Shanxi province.

[Spatial and temporal variations of hydrological characteristic on the landscape zone scale in alpine cold region].

There are few studies on the hydrological characteristics on the landscape zone scale in alpine cold region at present. This paper aimed to identify the spatial and temporal variations in the origin and composition of the runoff, and to reveal the hydrological characteristics in each zone, based on the isotopic analysis of glacier, snow, frozen soil, groundwater, etc. The results showed that during the wet season, heavy precipitation and high temperature in the Mafengou River basin caused secondary evaporation which led to isotope fractionation effects. Therefore, the isotope values remained high. Temperature effects were significant. During the dry season, the temperature was low. Precipitation was in the solid state during the cold season and the evaporation was weak. Water vapor came from the evaporation of local water bodies. Therefore, less secondary evaporation and water vapor exchange occurred, leading to negative values of delta18O and deltaD. delta18O and deltaD values of precipitation and various water bodies exhibited strong seasonal variations. Precipitation exhibited altitude effects, delta18O = -0. 005 2H - 8. 951, deltaD = -0.018 5H - 34. 873. Other water bodies did not show altitude effects in the wet season and dry season, because the runoff was not only recharged by precipitation, but also influenced by the freezing and thawing process of the glacier, snow and frozen soil. The mutual transformation of precipitation, melt water, surface water and groundwater led to variations in isotopic composition. Therefore, homogenization and evaporation effect are the main control factors of isotope variations.

Sensitivity of the reference evapotranspiration to key climatic variables during the growing season in the Ejina oasis northwest China.

The standardized FAO56 Penman-Monteith model, which has been the most reasonable method in both humid and arid climatic conditions, provides reference evapotranspiration (ETo) estimates for planning and efficient use of agricultural water resources. And sensitivity analysis is important in understanding the relative importance of climatic variables to the variation of reference evapotranspiration. In this study, a non-dimensional relative sensitivity coefficient was employed to predict responses of ETo to perturbations of four climatic variables in the Ejina oasis northwest China. A 20-year historical dataset of daily air temperature, wind speed, relative humidity and daily sunshine duration in the Ejina oasis was used in the analysis. Results have shown that daily sensitivity coefficients exhibited large fluctuations during the growing season, and shortwave radiation was the most sensitive variable in general for the Ejina oasis, followed by air temperature, wind speed and relative humidity. According to this study, the response of ETo can be preferably predicted under perturbation of air temperature, wind speed, relative humidity and shortwave radiation by their sensitivity coefficients.

Macroporous 'bubble' graphene film via template-directed ordered-assembly for high rate supercapacitors.

A three-dimensional bubble graphene film, with controllable and uniform macropores and tailorable microstructure, was fabricated by a facile hard templating strategy and exhibit extraordinary electrochemical capacitance with high rate capability (1.0 V s(-1)).