ABSTRACT
Cryptochromes are blue-light receptors that mediate blue-light inhibition of hypocotyl elongation and blue-light stimulation of floral initiation in Arabidopsis. In addition to their blue-light-dependent functions, cryptochromes are also involved in blue-light-independent regulation of the circadian clock, cotyledon unfolding, and hypocotyl inhibition. However, the molecular mechanism associated with the blue-light-independent function of cryptochromes remains unclear. We reported here a comparative proteomics study of the light regulation of protein expression. We showed that, as expected, the protein expression of many metabolic enzymes changed in response to both blue light and red light. Surprisingly, some light-regulated protein expression changes are impaired in the cry1cry2 mutant in both blue light and red light. This result suggests that, in addition to mediating blue-light-dependent regulation of protein expression, cryptochromes are also involved in the blue-light-independent regulation of gene expression. Consistent with this hypothesis, the cry1cry2 mutant exhibited reduced changes of mRNA expression in response to not only blue light, but also red light, although the cryptochrome effects on the red-light-dependent gene expression changes are generally less pronounced. These results support a hypothesis that, in addition to their blue-light-specific functions, cryptochromes also play roles in the control of gene expression mediated by the red/far-red-light receptor phytochromes.
Subject(s)
Arabidopsis/genetics , Arabidopsis/metabolism , Cryptochromes/genetics , Cryptochromes/metabolism , Gene Expression Regulation, Plant/radiation effects , Light , RNA, Messenger/genetics , RNA, Plant/genetics , Arabidopsis/radiation effects , Arabidopsis Proteins/genetics , Arabidopsis Proteins/metabolism , Arabidopsis Proteins/radiation effects , Carbonic Anhydrases/genetics , Carbonic Anhydrases/metabolism , Carbonic Anhydrases/radiation effects , Cryptochromes/radiation effects , Deoxyribodipyrimidine Photo-Lyase/genetics , Deoxyribodipyrimidine Photo-Lyase/metabolism , Deoxyribodipyrimidine Photo-Lyase/radiation effects , Kinetics , Mutagenesis , Mutation , Seedlings/genetics , Seedlings/metabolism , Seedlings/radiation effects , Spectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationABSTRACT
With incubation test, this paper studied the effects of fine root decomposition of Alnus cremastogyne, Cunninghamia lanceolata and Michelia macclurei on the content of soil active organic carbon at 9 degrees C , 14 degrees C , 24 degrees C and 28 degrees C. The results showed that the decomposition rate of fine root differed significantly with test tree species, which was decreased in the order of M. macclurei > A. cremastogyne > C. lanceolata. The decomposition rate was increased with increasing temperature, but declined with prolonged incubation time. Fine root source, incubation temperature, and incubation time all affected the contents of soil microbial biomass carbon and water-soluble organic carbon. The decomposition of fine root increased soil microbial biomass carbon and water-soluble organic carbon significantly, and the effect decreased in the order of M. macclurei > A. cremastogyne > C. lanceolata. Higher contents of soil microbial biomass carbon and water-soluble organic carbon were observed at medium temperature and middle incubation stage. Fine root decomposition had less effect on the content of soil readily oxidized organic carbon.
Subject(s)
Alnus/metabolism , Carbon/analysis , Cunninghamia/metabolism , Fagaceae/metabolism , Soil/analysis , Organic Chemicals/analysis , Plant Roots/metabolism , Soil MicrobiologyABSTRACT
OBJECTIVE: We report a case of mixed infection of Strongyloides stercoralis and Clonorchis sinensis, the diagnosis of which was difficult due to lack of specific clinical and imaging features. Definitive diagnosis was finally reached on the basis of detection of the larvae and eggs in the stool of the patient. Timely and effective therapy is of much importance for the prognosis of the disease.
Subject(s)
Clonorchiasis/complications , Strongyloides stercoralis , Strongyloidiasis/complications , Strongyloidiasis/diagnosis , Adult , Animals , Clonorchiasis/diagnosis , Clonorchiasis/drug therapy , Humans , Male , Strongyloidiasis/drug therapyABSTRACT
It is becoming increasingly evident that remarkable similaries of heart development are revealed in Drosophila and vertebrate, therefore Drosophila can be used as a prototype to explore the vertebrate. This can in accelerate to revealing of the machanisms of human heart development. In order to screen and clone new genes that control the heart development,we have established the balanced-lethal lines by chemical mutagen and performed the heart-specific antibody. Ten of lines showed mutant phenotype,of which 6 were determined the smaller genetic sites for gene location.
