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1.
Adv Sci (Weinh) ; : e2402450, 2024 Jul 01.
Article in English | MEDLINE | ID: mdl-38952061

ABSTRACT

Discovering new treatments for melanoma will benefit human health. The mechanism by which deoxyhypusine synthase (DHPS) promotes melanoma development remains elucidated. Multi-omics studies have revealed that DHPS regulates m6A modification and maintains mRNA stability in melanoma cells. Mechanistically, DHPS activates the hypusination of eukaryotic translation initiation factor 5A (eIF5A) to assist METTL3 localizing on its mRNA for m6A modification, then promoting METTL3 expression. Structure-based design, synthesis, and activity screening yielded the hit compound GL-1 as a DHPS inhibitor. Notably, GL-1 directly inhibits DHPS binding to eIF5A, whereas GC-7 cannot. Based on the clarification of the mode of action of GL-1 on DHPS, it is found that GL-1 can promote the accumulation of intracellular Cu2+ to induce apoptosis, and antibody microarray analysis shows that GL-1 inhibits the expression of several cytokines. GL-1 shows promising antitumor activity with good bioavailability in a xenograft tumor model. These findings clarify the molecular mechanisms by which DHPS regulates melanoma proliferation and demonstrate the potential of GL-1 for clinical melanoma therapy.

2.
Heliyon ; 10(1): e23770, 2024 Jan 15.
Article in English | MEDLINE | ID: mdl-38192823

ABSTRACT

Research on venous thromboembolism (VTE) in nursing has garnered significant attention. This study aimed to examine the characteristics of VTE nursing publications, offering valuable insights into the current state of the field and forecasting future trends. A comprehensive screening of global publications up to 2022 was conducted using the Web of Science Core Collection database to investigate VTE nursing. The search incorporated keywords such as 'venous thromboembolism', 'deep vein thrombosis', and 'pulmonary embolism' to identify relevant studies. A bibliometric analysis of these publications was performed using various visualisation tools such as VOSviewer and R software. A total of 675 papers on VTE nursing were identified, with the earliest publication dating back to 1999. The research involved 971 institutions from 43 countries, with the United States leading by contributing to 261 articles. Harvard University emerged as the most productive institution, and Heit, with 17 publications, was the most cited author. The journal Thrombosis Research published the highest number of papers (11). The frontiers of VTE nursing research are anticipated to continue focusing on topics such as epidemiology, risk factors, and VTE prevention and management.

3.
Biomed Pharmacother ; 167: 115440, 2023 Nov.
Article in English | MEDLINE | ID: mdl-37683595

ABSTRACT

The discovery of new therapeutic strategies for diseases is essential for drug research. Deoxyhypusine synthase (DHPS) is a critical enzyme that modifies the conversion of the eukaryotic translation initiation factor 5A (eIF5A) precursor into physiologically active eIF5A (eIF5A-Hyp). Recent studies have revealed that the hypusine modifying of DHPS on eIF5A has an essential regulatory role in human diseases. The hypusination-induced DHPS/eIF5A pathway has been shown to play an essential role in various cancers, and it could regulate immune-related diseases, glucose metabolism-related diseases, neurological-related diseases, and aging. In addition, DHPS has a more defined substrate and a well-defined structure within the active pocket than eIF5A. More and more researchers are focusing on the prospect of advanced development of DHPS inhibitors. This review summarizes the regulatory mechanisms of the hypusination-induced DHPS/eIF5A pathway in a variety of diseases in addition to the inhibitors related to this pathway; it highlights and analyzes the structural features and mechanisms of action of DHPS inhibitors and expands the prospects of future drug development using DHPS as an anticancer target.

4.
Mol Cancer ; 21(1): 177, 2022 09 08.
Article in English | MEDLINE | ID: mdl-36071472

ABSTRACT

Given that hypoxia is a persistent physiological feature of many different solid tumors and a key driver for cancer malignancy, it is thought to be a major target in cancer treatment recently. Tumor-associated macrophages (TAMs) are the most abundant immune cells in the tumor microenvironment (TME), which have a large impact on tumor development and immunotherapy. TAMs massively accumulate within hypoxic tumor regions. TAMs and hypoxia represent a deadly combination because hypoxia has been suggested to induce a pro-tumorigenic macrophage phenotype. Hypoxia not only directly affects macrophage polarization, but it also has an indirect effect by altering the communication between tumor cells and macrophages. For example, hypoxia can influence the expression of chemokines and exosomes, both of which have profound impacts on the recipient cells. Recently, it has been demonstrated that the intricate interaction between cancer cells and TAMs in the hypoxic TME is relevant to poor prognosis and increased tumor malignancy. However, there are no comprehensive literature reviews on the molecular mechanisms underlying the hypoxia-mediated communication between tumor cells and TAMs. Therefore, this review has the aim to collect all recently available data on this topic and provide insights for developing novel therapeutic strategies for reducing the effects of hypoxia.


Subject(s)
Neoplasms , Tumor-Associated Macrophages , Humans , Hypoxia/metabolism , Macrophages/metabolism , Neoplasms/pathology , Tumor Microenvironment
5.
Int J Pharm ; 611: 121301, 2022 Jan 05.
Article in English | MEDLINE | ID: mdl-34793933

ABSTRACT

A bioadhesive nanocarrier, PTNP, was constructed by utilizing a novel poly(methyl vinyl ether-co-maleic anhydride)- D-α-Tocopheryl polyethylene glycol succinate (PVMMA-TPGS) copolymer in the PLGA/lipid hybrid nanoparticles (PLGA NPs) for improving oral delivery of cabazitaxel (CTX). The PVMMA-TPGS was synthesized by the ring-opening polymerization of the anhydride groups with the hydroxyl groups, combining the bioadhesive property of PVMMA with P-glycoprotein (P-gp) inhibitory effect of TPGS. The CTX-loaded PTNPs (CTX-PTNPs) were prepared by an emulsification-solvent evaporation method and performed a spherical appearance with a uniform particle size of 192.2 nm. The CTX-PTNPs were surface negatively charged, and exhibited good drug loading (10.2%) and encapsulation efficiency (92.1%). A sustained drug release and high stability in simulated gastrointestinal environment were confirmed in in vitro studies. The in vitro mucin adhesion and in vivo intestinal retention experiments indicated that the PTNPs had a stronger bioadhesive effect and a notably longer intestinal retention than the control PLGA NPs, due to the interaction of PVMMA on the PTNP surface with the intestinal mucosa. Moreover, an enhanced intestinal permeability of the PTNPs was also verified in in vivo and ex vivo intestinal permeation studies, which was probably attributed to the extended retention of PTNPs in intestinal mucosa and the P-gp inhibitory effect of TPGS. As respected, in in vivo pharmacokinetic study, the Tmax and oral bioavailability of CTX were dramatically improved to 1.08 h and 28.84% by the PTNPs, respectively, obviously superior to the CTX solution and the PLGA NPs, further demonstrating the high-efficiency in oral delivery of CTX. Hence, this bioadhesive carrier is proposed to be a potential and promising strategy for increasing oral absorption of small molecule insoluble drugs.


Subject(s)
Nanoparticles , Intestinal Absorption , Lipids , Maleates , Polyethylenes , Taxoids , Vitamin E
6.
Int J Pharm ; 601: 120583, 2021 May 15.
Article in English | MEDLINE | ID: mdl-33839225

ABSTRACT

Oral delivery of exenatide (EXE), a high-efficiency therapeutic peptide, is urgently needed for long-term treatment of diabetes. In this study, a polylactide-co-glycoside (PLGA) nanoparticles (NPs) in yeast cell wall particle (YCWP) system was built to improve the intestinal absorption of EXE by efficient protection of EXE against gastrointestinal degradation and intestinal phagocytic cell targeted delivery. The EXE-loaded PLGA NPs were prepared by a double emulsion solvent diffusion method and exhibited a uniformly spherical appearance, a nano size (92.4 ± 4.6 nm) and a positive surface charge (+32.3 ± 3.8 mV). And then, the NPs were successfully loaded into the YCWPs by a solvent hydration - lyophilization cycle method to obtain the EXE-PLGA NPs @YCWPs, which was verified by scanning electron microscope and confocal laser scanning microscopy. An obvious sustained drug release and a reduced burst release were achieved by this nano-in-micro carrier. Moreover, the gastrointestinal stability of EXE in PLGA NPs @YCWPs was significantly higher than that in PLGA NPs in the simulated gastrointestinal environment, which were useful in enhancing the intestinal absorption of EXE. In biodistribution study, the EXE-PLGA NPs @YCWPs could quickly reached the root of the villi, and even partly entered the inner of the villi, especially in ileum and Peyer's patches. In vitro cell evaluation demonstrated an efficient ß-glucan receptor mediated endocytosis and transport of EXE-PLGA NPs @YCWPs by the macrophage RAW 264.7 cells, suggesting a potential intestinal macrophage targeted absorptive pathway. The in vivo pharmacokinetic study showed a preferred hypoglycemic effect and an increased pharmacological availability (13.7 ± 4.1%) after oral administration of the EXE-PLGA NPs @YCWPs. It is believed that the PLGA nanoparticles in YCWP system could become an efficient strategy to orally deliver therapeutic peptide drugs.


Subject(s)
Hypoglycemic Agents , Nanoparticles , Cell Wall , Drug Carriers , Exenatide , Particle Size , Polylactic Acid-Polyglycolic Acid Copolymer , Saccharomyces cerevisiae , Tissue Distribution
7.
J Clin Invest ; 127(5): 1741-1756, 2017 May 01.
Article in English | MEDLINE | ID: mdl-28375159

ABSTRACT

The integration of somatosensory information is generally assumed to be a function of the central nervous system (CNS). Here we describe fully functional GABAergic communication within rodent peripheral sensory ganglia and show that it can modulate transmission of pain-related signals from the peripheral sensory nerves to the CNS. We found that sensory neurons express major proteins necessary for GABA synthesis and release and that sensory neurons released GABA in response to depolarization. In vivo focal infusion of GABA or GABA reuptake inhibitor to sensory ganglia dramatically reduced acute peripherally induced nociception and alleviated neuropathic and inflammatory pain. In addition, focal application of GABA receptor antagonists to sensory ganglia triggered or exacerbated peripherally induced nociception. We also demonstrated that chemogenetic or optogenetic depolarization of GABAergic dorsal root ganglion neurons in vivo reduced acute and chronic peripherally induced nociception. Mechanistically, GABA depolarized the majority of sensory neuron somata, yet produced a net inhibitory effect on the nociceptive transmission due to the filtering effect at nociceptive fiber T-junctions. Our findings indicate that peripheral somatosensory ganglia represent a hitherto underappreciated site of somatosensory signal integration and offer a potential target for therapeutic intervention.


Subject(s)
GABA Uptake Inhibitors/adverse effects , GABAergic Neurons/metabolism , Ganglia, Spinal , Neuralgia , Nociception/drug effects , Synaptic Transmission/drug effects , Animals , GABA Uptake Inhibitors/pharmacology , Ganglia, Spinal/metabolism , Ganglia, Spinal/pathology , Ganglia, Spinal/physiopathology , Neuralgia/chemically induced , Neuralgia/metabolism , Neuralgia/pathology , Neuralgia/physiopathology , Rats , Rats, Sprague-Dawley , Rats, Wistar
8.
Cell Biochem Biophys ; 71(1): 155-60, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25151141

ABSTRACT

The objective of this paper was to prepare nifedipine-loaded oral copolymer micelles and to improve bioavailability of hydrophobic drugs. The methoxy poly(ethylene glycol)-b-polycaprolactone diblock copolymer (mPEG-b-PCL) we developed was the research object; solvent evaporation method was utilized to prepare nifedipine-loaded copolymer micelles, and the drug concentration, drug-loaded amount, and entrapment efficiency were also determined. Transmission electron microscopy and dynamic light scattering were used to characterize the morphology and size distributions of micelles, and the in vivo pharmacokinetics were studied in rats with the research objects of nifedipine-loaded oral copolymer micelles. The drug concentration, drug-loaded amount, and entrapment efficiency of mPEG-b-PCL-nifedipine micelles were (69.39 ± 4.33) µg mL(-1), (3.35 ± 0.21)%, and (8.67 ± 0.54)%, respectively. The micelles were globular shaped with a narrow size distribution and a mean diameter of (34.8 ± 3.2) nm, and the relative bioavailability of the micelles we developed was 246.20% when compared with the tablets available in the market. The mPEG-b-PCL-nifedipine oral copolymer micelles can improve the bioavailability of hydrophobic drugs. Oral polymer micelles drug delivery system has a good prospect.


Subject(s)
Drug Carriers/chemistry , Micelles , Nifedipine/administration & dosage , Nifedipine/pharmacokinetics , Polyesters/chemistry , Polyethylene Glycols/chemistry , Absorption, Physicochemical , Administration, Oral , Animals , Drug Compounding , Feasibility Studies , Hydrophobic and Hydrophilic Interactions , Male , Particle Size , Rats , Water/chemistry
9.
Pain ; 155(11): 2306-22, 2014 Nov.
Article in English | MEDLINE | ID: mdl-25168672

ABSTRACT

Peripheral sensory ganglia contain somata of afferent fibres conveying somatosensory inputs to the central nervous system. Growing evidence suggests that the somatic/perisomatic region of sensory neurons can influence peripheral sensory transmission. Control of resting membrane potential (Erest) is an important mechanism regulating excitability, but surprisingly little is known about how Erest is regulated in sensory neuron somata or how changes in somatic/perisomatic Erest affect peripheral sensory transmission. We first evaluated the influence of several major ion channels on Erest in cultured small-diameter, mostly capsaicin-sensitive (presumed nociceptive) dorsal root ganglion (DRG) neurons. The strongest and most prevalent effect on Erest was achieved by modulating M channels, K2P and 4-aminopiridine-sensitive KV channels, while hyperpolarization-activated cyclic nucleotide-gated, voltage-gated Na(+), and T-type Ca(2+) channels to a lesser extent also contributed to Erest. Second, we investigated how varying somatic/perisomatic membrane potential, by manipulating ion channels of sensory neurons within the DRG, affected peripheral nociceptive transmission in vivo. Acute focal application of M or KATP channel enhancers or a hyperpolarization-activated cyclic nucleotide-gated channel blocker to L5 DRG in vivo significantly alleviated pain induced by hind paw injection of bradykinin. Finally, we show with computational modelling how somatic/perisomatic hyperpolarization, in concert with the low-pass filtering properties of the t-junction within the DRG, can interfere with action potential propagation. Our study deciphers a complement of ion channels that sets the somatic Erest of nociceptive neurons and provides strong evidence for a robust filtering role of the somatic and perisomatic compartments of peripheral nociceptive neuron.


Subject(s)
Membrane Potentials/physiology , Nociceptors/physiology , Animals , Animals, Newborn , Bradykinin/pharmacology , Calcium Channels, T-Type/genetics , Calcium Channels, T-Type/metabolism , Carbamates/pharmacology , Cells, Cultured , Cricetulus , Ganglia, Spinal/cytology , Humans , In Vitro Techniques , Ion Channels/genetics , Ion Channels/metabolism , KCNQ2 Potassium Channel/genetics , KCNQ2 Potassium Channel/metabolism , KCNQ3 Potassium Channel/genetics , KCNQ3 Potassium Channel/metabolism , Male , Membrane Potentials/drug effects , Membrane Transport Modulators , Models, Neurological , Nociceptors/drug effects , Phenylenediamines/pharmacology , Rats , Rats, Sprague-Dawley , Spectrophotometry, Atomic
10.
Lipids Health Dis ; 12: 154, 2013 Oct 25.
Article in English | MEDLINE | ID: mdl-24160562

ABSTRACT

BACKGROUND: To investigate the anti-hyperlipidemic effect of gynosaponins (GPs) in hyperlipidemic rats induced by high lipid diet. METHODS: Animal model of hyperlipidemia was established by high-fat and high-cholesterol diet. Rats were randomly divided into 6 groups, except the normal and model groups, rats in GPs groups were daily administered intragastrically with GPs (50, 100, and 200 mg/kg), and rats in simvastatin group were daily administered intragastrically with simvastatin (10 mg/kg). It was measured that the contents of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), total cholesterol (TC), triglyceride (TG), high-density lipoprotein-cholesterol (HDL-C) and low-density lipoprotein-cholesterol (LDL-C) in the serum, TG and TC in the liver during this experiment, respectively. The left lobe of liver was observed by histopathological staining, and the immunohistochemical staining was used to observe the effects on the effect of GPs on liver functions. RESULTS: Compared with the model group, GPs groups could remarkably decrease the content of lipids, GSH-Px, SOD, CAT and MDA in the serum and TC and TG in the liver of the hyperlipidemic rats. The pathomorphological results of hepatic tissue showed that fatty degeneration and inflammatory reaction of GPs groups were lightened compared with the model group. CONCLUSIONS: The results show that GPs has good effects on the treatment of hyperlipidemia induced by high lipid diet in rats. The possible anti-hyperlipidemia mechanism maybe those GPs can regulate the disorder of lipid metabolism as well as ameliorate hepatic function.


Subject(s)
Anticholesteremic Agents/pharmacology , Diet, High-Fat , Hypercholesterolemia/drug therapy , Lipid Metabolism/drug effects , Liver/drug effects , Animals , Catalase/metabolism , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Dietary Fats/adverse effects , Glutathione Peroxidase/metabolism , Gynostemma , Hypercholesterolemia/etiology , Hypercholesterolemia/metabolism , Hypercholesterolemia/pathology , Liver/metabolism , Liver/pathology , Male , Malondialdehyde/metabolism , Plant Extracts/pharmacology , Rats , Rats, Wistar , Simvastatin/pharmacology , Superoxide Dismutase/metabolism , Triglycerides/blood
11.
Eur J Pharm Biopharm ; 80(2): 332-9, 2012 Feb.
Article in English | MEDLINE | ID: mdl-22041601

ABSTRACT

Antitumor drugs not only cause cytocidal effect on cancer cells, but also damage on normal healthy tissues, resulting in side effects. Liposome encapsulation can result in reduced systematic distribution due to the enhanced permeability and retention (EPR) effect, accompanied by drug accumulation in liver, spleen, and other immune organs, which can cause damage to those organs. It has been demonstrated that EDTA, frequently used as a chelator, possesses a synergistic antitumor effect. Indeed, our previous study showed that EDTA could reduce the toxicity of anthracyclines to the heart and immune organs. In this study, we intended to encapsulate topotecan within liposome adopting transmembrane NH(4)EDTA gradient in order to increase the antitumor activity and decrease the toxicity against normal immune organs. Regarding the encapsulation efficiency of topotecan liposomes, both the pH value of the buffer and the cholesterol content showed significant effects on encapsulation and drug retention. Liposome encapsulation dramatically increased the antitumor activity of topotecan compared to free drug (p<0.05), while similar efficacy was obtained from liposomes prepared by a NH(4)EDTA gradient or a (NH(4))(2)SO(4) gradient (tumor inhibition ratios were 85.6% and 84.1%, respectively). However, a significant decrease in toxicity against the immune organs was found in liposomes prepared by a NH(4)EDTA gradient compared to those prepared by a (NH(4))(2)SO(4) gradient. These results suggest the superiority of the proposed gradient for topotecan encapsulation in decreasing its toxicity on immune systems.


Subject(s)
Antineoplastic Agents/administration & dosage , Cholesterol/chemistry , Edetic Acid/chemistry , Topotecan/administration & dosage , Animals , Antineoplastic Agents/pharmacology , Antineoplastic Agents/toxicity , Dogs , Humans , Hydrogen-Ion Concentration , Liposomes , Liver Neoplasms, Experimental/drug therapy , Liver Neoplasms, Experimental/pathology , Male , Mice , Quaternary Ammonium Compounds/chemistry , Topotecan/pharmacology , Topotecan/toxicity , Toxicity Tests, Acute , Xenograft Model Antitumor Assays
12.
Zhongguo Zhong Yao Za Zhi ; 30(15): 1154-6, 2005 Aug.
Article in Chinese | MEDLINE | ID: mdl-16201687

ABSTRACT

OBJECTIVE: To study the solubilization effect of 2-hydroxypropyl-beta-cyclodextrin(HPCD) on paeonolum at various pH value. METHOD: Phase-solubility method was adopted to study the solubilization effect at 25 degrees C and UV spectrohotometer was used to determine paeonolum content. RESULTS: The apparent solubility of paeonolum was significantly enhanced by increased HPCD concentration. The apparent stability constant of paeonolum compounds was calculated up to 1 425 in which pH was 3 and HPCD concentration was 133.33 mmol x L(-1). The solubility of paeonolum came up to 10 mg x mL(-1). CONCLUSION: HPCD is an ideal solubilizer for paeonolum.


Subject(s)
Acetophenones/administration & dosage , Paeonia , beta-Cyclodextrins , 2-Hydroxypropyl-beta-cyclodextrin , Acetophenones/chemistry , Acetophenones/isolation & purification , Drug Stability , Excipients , Hydrogen-Ion Concentration , Paeonia/chemistry , Plants, Medicinal/chemistry , Solubility
13.
Zhongguo Zhong Yao Za Zhi ; 30(20): 1591-3, 2005 Oct.
Article in Chinese | MEDLINE | ID: mdl-16422539

ABSTRACT

OBJECTIVE: To study the chemical constituents of Fagopyrum cymosum. METHOD: The chemical constituents were isolated by various chromatographic methods and their structures were elucidated by the analysis of spectral data and physiochemical properties. RESULT: Four phenolic acid derivatives were isolated from F. cymosum. The chemical structures were elucidated as trans-p-hydroxy cinnamic methyl ester (I), 3, 4-dihydroxy benzamide (II), protocatechuic acid (III), protocatechuic acid methyl ester (IV). CONCLUSION: Compounds I, II, IV were obtained from the genus Fagopyrum for the first time.


Subject(s)
Benzamides/isolation & purification , Cinnamates/isolation & purification , Fagopyrum/chemistry , Plants, Medicinal/chemistry , Benzamides/chemistry , Cinnamates/chemistry , Hydroxybenzoates/chemistry , Hydroxybenzoates/isolation & purification , Rhizome/chemistry
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