Enhanced bioelectroremediation of heavy metal contaminated groundwater through advancing a self-standing cathode.

Hexavalent chromium (Cr(VI)) contamination in groundwater poses a substantial global challenge due to its high toxicity and extensive industrial applications. While the bioelectroremediation of Cr(VI) has attracted huge attention for its eco-friendly attributes, its practical application remains constrained by the hydrogeochemical conditions of groundwater (mainly pH), low electron transfer efficiency, limitations in electrocatalyst synthesis and electrode fabrication. In this study, we developed and investigated the use of N, S co-doped carbon nanofibers (CNFs) integrated on a graphite felt (GF) as a self-standing cathode (NS/CNF-GF) for the comprehensive reduction of Cr(VI) from real contaminated groundwater. The binder free cathode, prepared through electro-polymerization, was employed in a dual-chamber microbial fuel cell (MFC) for the treatment of Cr (VI)-laden real groundwater (40 mg/L) with a pH of 7.4. The electrochemical characterization of the prepared cathode revealed a distinct electroactive surface area, more wettability, facilitating enhanced adsorption and rapid electron transfer, resulting in a commendable Cr(VI) reduction rate of 0.83 mg/L/h. The MFC equipped with NS/CNF-GF demonstrated the lowest charge transfer resistance (Rct) and generated the highest power density (155 ± 0.3 mW/m2) compared to control systems. The favorable electrokinetics for modified cathode led to swift substrate consumption in the anode, releasing more electrons and protons, thereby accelerating Cr(VI) reduction to achieve the highest cathodic coulombic efficiency (C.Eca)of80 ± 1.3 %. A similar temporal trend observed between Cr(VI) removal efficiency, COD removal efficiency, and C.Eca, underscores the effective performance of the modified electrode. The reusability of the binder free cathode, exemption from catholyte preparation and the absence of pH regulation requirements highlighted the potential scalability and applicability of our findings on a larger scale.

Processes and mechanisms in remediation of aqueous chromium contamination by sulfidated nano-scale zerovalent iron (S-nZVI): Experimental and computational investigations.

Sulfidated nano-scale zerovalent iron (S-nZVI) has emerged as an advanced functional nanomaterial for efficiently remediating Cr(VI) contamination in aqueous environments. However, there is an insufficient understanding of its coherent process, removal pathway, and hydrochemical reactive mechanisms, presenting potential challenges for its future environmental applications. To address this gap, this study successfully synthesized S-nZVI through a chemical precipitation method and effectively applied it for the removal of Cr(VI). Additional characterization revealed that the removal of Cr(VI) followed a sequence of rapid chemisorption and intraparticle diffusion processes, concomitant with an increase in pH and a decrease in oxidation-reduction potential. The remediation mechanism encompassed a synergistic reduction of Cr(VI) to Cr(III) and simultaneous immobilization via Cr2FeO4 coprecipitation. The highest Cr(VI) removal capacity of 75 mg/g was attained during dynamic removal experiments in the sand column packed with S-nZVI. Further computational analysis, employing density functional theory calculations based on the experimental data, revealed the involvement of multiple molecular orbitals of Cr(VI) in the removal process. It also elucidated a step-by-step reduction pathway for Cr(VI) characterized by decreasing free energy. These findings provide evidence-based insights into Cr(VI) remediation using S-nZVI and can serve as valuable technical support for future environmental management of heavy metals.

E. coli cotransport with composite colloid in unsaturated porous media: Multi-risk on migration and biomolecular response.

The diversity of colloidal types and the differences in the composite ratios in porous media are important factors governing the migration and biological risk of pathogenic microorganisms in the subsurface environment. In this study, E. coli O157:H7 was subjected to co-migration experiments with different compositions of the composite colloid montmorillonite (MMT)-Fe2O3, and the biomolecular response of E. coli under the action of colloids was analyzed by Raman spectroscopy to quantify the risk of E. coli under the action of composite colloids based on both. The results showed that Fe2O3 colloids inhibited E. coli migration mainly by electrostatic adsorption and reduced E. coli metabolism. MMT colloid inhibited E. coli migration mainly by blockage, and E. coli metabolism increased, and surface macromolecules decreased to reduce E. coli adhesion. MMT-Fe2O3 complex colloids inhibited migration through electrostatic attraction between the two and formation of cohesive colloids, with reduced E. coli metabolism and insignificant biomolecular response. It was briefly assessed that the composite colloids reduced E. coli risk less strongly than single colloids, stemming from the difference in the mechanism of influence and the actual need to consider colloid interactions. This conclusion can inform the management and control of pathogen risk in porous media environments.

Facilitating New Chromium Reducing Microbes to Enhance Hexavalent Chromium Reduction by In Situ Sonoporation-Mediated Gene Transfer in Soils.

Chromium (Cr) is a heavy metal with a high toxicity and pathogenicity. Microbial reduction is an effective strategy to remove Cr(VI) at contaminated sites but suffers from the low populations and activities of Cr-reducing microorganisms in soils. This study proposed an in situ sonoporation-mediated gene transfer approach, which improved soil Cr(VI) reduction performance by delivering exogenous Cr-transporter chrA genes and Cr-reducing yieF genes into soil microorganisms with the aid of ultrasound. Besides the increasing populations of Cr-resistant bacteria and elevated copy numbers of chrA and yieF genes after sonoporation-mediated gene transfer, three new Cr-reducing strains were isolated, among which Comamonas aquatica was confirmed to obtain Cr-resistant capability. In addition, sonoporation-mediated gene transfer was the main driving force significantly shaping soil microbial communities owing to the predominance of Cr-resistant microbes. This study pioneered and evidenced that in situ soil sonoporation-mediated gene transfer could effectively deliver functional genes into soil indigenous microbes to facilitate microbial functions for enhanced bioremediation, e.g., Cr-reduction in this study, showing its feasibility as a chemically green and sustainable remediation strategy for heavy metal contaminated sites.

Migration risk of Escherichia coli O157:H7 in unsaturated porous media in response to different colloid types and compositions.

The vadose zone is a critical zone for microbial entry into the subsurface environment, and various types of inorganic and organic colloids can affect the migration of pathogenic bacteria. In the study, we explored the migration behavior of Escherichia coli O157:H7 with humic acids (HA), iron oxides (Fe2O3) or their mixture, uncovering their migration mechanisms in the vadose zone. The effect of complex colloids on the physiological properties of E. coli O157:H7 was analyzed based on the measured particle size, zeta potential and contact angle. HA colloids significantly promoted the migration of E. coli O157:H7, where Fe2O3 was opposite. The migration mechanism of E. coli O157:H7 with HA and Fe2O3 is obviously different. Multiple colloids dominated by organic colloid will further highlight its promoting effect on E. coli O157:H7 under the guidance of electrostatic repulsion due to the influence of colloidal stability. Multiple colloids dominated by metallic colloid will inhibit the migration of E. coli O157:H7 under the control of capillary force due to the restriction of contact angle. The risk of secondary release of E. coli O157:H7 can be effectively reduced when the ratio of HA/Fe2O3 is ≥ 1. Combining this conclusion with the distribution characteristics of soil in China, an attempt was made to analyse the migration risk of E. coli O157:H7 on a national scale. In China, from north to south, the migration capacity of E. coli O157:H7 gradually decreased, and the risk of secondary release gradually increased. These results provide ideas for the subsequent study of the effect of other factors on the migration of pathogenic bacteria on a national scale and provide risk information about soil colloids for the construction of pathogen risk assessment model under comprehensive conditions in the future.

Oxygen micro-nanobubbles for mitigating eutrophication induced sediment pollution in freshwater bodies.

Sediment hypoxia is a growing problem and has negative ecological impacts on the aquatic ecosystem. Hypoxia can disturb the biodiversity and biogeochemical cycles of both phosphorus (P) and nitrogen (N) in water columns and sediments. Anthropogenic eutrophication and internal nutrient release from lakebed sediment accelerate hypoxia to form a dead zone. Thus, sediment hypoxia mitigation is necessary for ecological restoration and sustainable development. Conventional aeration practices to control sediment hypoxia, are not effective due to high cost, sediment disturbance and less sustainability. Owing to high solubility and stability, micro-nanobubbles (MNBs) offer several advantages over conventional water and wastewater treatment practices. Clay loaded oxygen micro-nanobubbles (OMNBs) can be delivered into deep water sediment by gravity and settling. Nanobubble technology provides a promising route for cost-effective oxygen delivery in large natural water systems. OMNBs also have the immense potential to manipulate biochemical pathways and microbial processes for remediating sediment pollution in natural waters. This review article aims to analyze recent trends employing OMNBs loaded materials to mitigate sediment hypoxia and subsequent pollution. The first part of the review highlights various minerals/materials used for the delivery of OMNBs into benthic sediments of freshwater bodies. Release of OMNBs at hypoxic sediment water interphase (SWI) can provide significant dissolved oxygen (DO) to remediate hypoxia induced sediment pollution Second part of the manuscript unveils the impacts of OMNBs on sediment pollutants (e.g., methylmercury, arsenic, and greenhouse gases) remediation and microbial processes for improved biogeochemical cycles. The review article will facilitate environmental engineers and ecologists to control sediment pollution along with ecological restoration.

Natural colloids facilitated transport of steroidal estrogens in saturated porous media: Mechanism and processes.

Steroid estrogens (SEs) as typical endocrine disrupting compounds (EDCs) are widely detected in terrestrial environment, whilst the transport of SEs in groundwater remains unwell understood. Specifically, the effects of ubiquitous natural colloids on the SEs transport are unclear in subsurface environment, especially in aquifer systems. Here, the influence of inorganic colloids (i.e. silica and illite) and organic colloids, i.e. Humic acid (HA), on the transport of estrone (E1) and estradiol (E2) in saturated porous media was studied utilizing laboratory scale column experiments. Characterization on the colloids and porous aquifer material was conducted to provide a basis for interpretation of the experimental findings. Results showed that the transport of SEs was clearly affected by the natural colloids migrating through the saturated porous media. About 38.5% of E1 and 24.6% of E2 were retained in the column when colloids were absent in the system. When transporting with silica colloids, illite colloids, and HA colloids, the transport of E1 was enhanced by 15.64%, 11.17% and 25.60%, respectively; whilst the transport of E2 was improved by 19.56%, 23.06% and 36.40%, respectively. The SEs transport enhancement by colloids depended upon not only the mobility of the colloids but also their geochemical characteristics. The organic colloids showed 1.5-2.5 times greater ability on promoting the transport of SEs than the inorganic ones tested in this study. The proposed mechanisms of nature colloids facilitated transport of SEs including competing for adsorption sites on the sand surfaces by the colloids resulting mobilization of adsorbed SEs from solid matrix, and transport of colloids as carriers for SEs.

The response of steroid estrogens bioavailability to various sorption mechanisms by soil organic matter extracted with sequential alkaline-extraction method from an agriculture soil.

The long-term groundwater contamination risks posed by steroidal estrogens (SEs) in animal-manured agricultural soils are closely associated with the soil organic matter (SOM) content and composition. In this study, the bioavailability of estrone (E1) and 17ß-estradiol (17ß-E2) under different sorption mechanism in humic acids (HA1 and HA2) and humin (HM) extracted with sequential alkaline-extraction technique (SAET) were examined. These SOMs extracted by SAET showed various properties and sorption characteristics for SEs. The alkyl carbon and condensed SOM increased during SAET, but aromatic carbon decreased and the same trend for polarity. Quick sorption was the major SEs sorption mechanism on HA1 and HA2, which contributed more than 69%; whilst slow sorption rate was about 50% in soil and HM. The logKoc values were proportional to the TOC of SOM according to Freundlich fitting, and the sorption capacity of sorbent for E1 and 17ß-E2 was related to the logKow values, indicating that the main mechanism controlling the SEs sorption was hydrophobic interaction. The larger micropore volume of HM and soil was more conducive to the micropore filling of SEs. Meanwhile, the specific sorption of SEs on condensed domain of SOM was the main reason for the strong desorption hysteresis and slow sorption in HM and soil. The SEs degradation rate was positively correlated with the contribution rate of quick adsorption and negatively correlated with the contribution rate of slow adsorption, indicating that the bioavailability of SEs sorbed by hydrophobic interaction was higher than that of micropore filling or specific sorption, which was also the reason for the low bioavailability of SEs in HM and soil. This work confirms the regulation of on-site SOM compositions and their properties on SEs sorption and bioavailability. Characterization of these details is crucial for the improved prediction of long-term risks to groundwater.

Stable Carbon Isotope Analysis of Hexachlorocyclohexanes by Liquid-Liquid Extraction Gas Chromatography Isotope Ratio Mass Spectrometry: Method Evaluation and Applications.

Compound specific isotope analysis (CSIA) and enantiomer specific isotope analysis (ESIA) are powerful tools for assessing the fate of hexachlorocyclohexanes (HCHs) in the environment. However, there is no systematic study on the CSIA and ESIA analysis test methods of the carbon isotopes of HCHs in water and soil environments, in particular the isotope fractionation in the pre-concentration process. We endeavored to test the compatibility of CSIA and ESIA with the liquid-liquid extraction method of HCHs in water. The results showed that there were negligible changes in the δ13C of HCHs after extraction, indicating that liquid-liquid extraction can be used as a pre-concentration method for the determination of δ13C of HCHs in water. The optimized method was validated and then applied to differentiate three HCHs from different manufacturers, to identify in situ degradation of HCHs of groundwater from a contaminated site and to resolve the carbon isotope fractionation occurring in the α-HCH oxidation by CaO2/Fe(II) Fenton system. The results showed that the same reagents from different manufacturers have different carbon isotope compositions, and different isomers from the same manufacturer also have different isotope compositions, showing useful evidence in identifying the source of HCHs. The more enriched δ13C in the down-gradient wells indicated that HCHs have undergone biodegradation or/and chemical reactions in the groundwater system of the site. Carbon isotopic enrichment factors (εC) of -1.90 ± 0.10‱ were obtained in the oxidation process. Hence, the method validated in this study has great potential as a method for identifying the degradation of HCHs in a water environment.

Decreased levels and ecological risks of disinfection by-product chloroform in a field-scale artificial groundwater recharge project by colloid supplement.

To bolster freshwater supply, artificial groundwater recharge with recycled water has increasingly attracted research attentions and interests. However, artificial groundwater recharge has potential risks to groundwater quality, as recharge water disinfection is frequently used for pathogen inactivation and causes the concerns of disinfection by-products (DBPs). Colloid supplement is a good approach solving this problem, but its roles in mitigating DBPs remains unclear. In this study, we collected 20 groundwater and soil samples from a field-scale groundwater recharge project, and explored the impacts of silica colloids on chloroform migration and groundwater bacterial communities during the recharge process. Water physicochemical variables changed along the recharge time, and colloid supplement significantly reduced chloroform formation and slowed its migration in groundwater. Bacterial communities in groundwater, river water and recharge water were significantly different. Gammaproteobacteria in recharge water (71.7%) was more abundant than in river water (30.5%) and groundwater (33.5%), while Actinobacteria dominated groundwater (40.6%). After recharge, Gammaproteobacteria increased more with colloid supplement (75.7%) than without (52.6%), attributing to its dominance in soils (74.6%). Our results suggested more bacterial lineages released from soils into aquifer by silica colloid supplement, owing to the competitive adsorption encouraging microbial transfer, especially Gram-negative bacteria. Our findings unraveled the effects of colloid supplement on chloroform formation and migration during artificial groundwater recharge, which consequently altered groundwater bacterial communities, and offered valuable suggestions for the safety management of DBPs in aquifer recharge.

Isolation of functional bacterial strains from chromium-contaminated site and bioremediation potentials.

In this study, two Cr(VI)-reducing functional bacterial strains (TJ-1 and TJ-5) were successfully isolated and screened from the chromium-contaminated soil from a real site. The 16S rRNA gene sequences were analysed, which showed high similarity (>99%) with Stenotrophomonas maltophilia (TJ-1) and Brucella intermedius (TJ-5) species. The optimum growth for the two bacteria to reduce Cr(VI) were achieved at pH 7.0 and initial inoculation amount of 5%. The two strains were applied to real contaminated soil samples and showed better Cr removal when external carbon sources were added. Using sawdust as a solid-phase carbon source supplement, both TJ-1 and TJ-5 showed higher remediation efficiency (99.77% and 93.86%) than using glucose as the carbon source (68.56% and 70.87%). Results of the stability of soil Cr(VI) bioremediation revealed that the water-soluble Cr(VI) content of bioremediated sample remained unchanged, indicating that Cr(VI) is not easily released after death of the strains. Solid-phase carbon source supplements may help the cells to attach and grow into biofilms, creating a better growth condition which improved the remediation efficiency. Column experiments showed that the total remediation efficiencies by the two strains were 34.23% and 20.63%, respectively, within a short time period (76 h). Therefore, the two strains showed great bioremediation potentials for chromium-contaminated sites and can be used in future application of in-situ bioremediation.

Influence of calcium and magnesium elimination on plant biomass and secondary metabolites of Stevia rebaudiana Bertoni.

This study reports the increment in the secondary metabolites in Stevia rebaudiana plant after exposure to the elimination of Ca and Mg from Murashige and Skoog culture medium. The effect of nutrient stress on regenerants of S. rebaudiana is measured, which reveals significantly enhanced growth parameters, steviol glycosides (SGs) content, and nonenzymatic antioxidants; total phenolic content, total flavonoid content, total antioxidant capacity, total reducing power, and DPPH-free radical scavenging activity as compared with the control treatment. However, significantly highest amounts are obtained in a medium with only Ca deficiency. The amount of rebaudioside A (Reb A) and stevioside (ST) obtained in the case of Ca-deficient medium is 4.08 and 0.69%, respectively. It is followed by the results obtained from both Ca- and Mg-deprived medium [Reb A (3.23%) and ST (0.52%)] and the lowest values are obtained from medium lacking Mg only [Reb A (2.60%) and ST (0.40%)]. The most probable adaptation mechanism might be the production of reactive oxygen species by nutrients' stress, which results in secondary metabolites production as defensive moieties to overcome stress situation. This effective protocol needs to be refined to apply on an industrial scale in bioreactors for increasing quantities of commercially important pharmaceutical compounds.

Extracellular Polymeric Substances Facilitate the Adsorption and Migration of Cu2+ and Cd2+ in Saturated Porous Media.

Heavy metal contamination in groundwater is a serious environmental problem. Many microorganisms that survive in subsurface porous media also produce extracellular polymeric substances (EPS), but little is known about the effect of these EPS on the fate and transport of heavy metals in aquifers. In this study, EPS extracted from soil with a steam method were used to study the adsorption behaviors of Cu2+ and Cd2+, employing quartz sand as a subsurface porous medium. The results showed that EPS had a good adsorption capacity for Cu2+ (13.5 mg/g) and Cd2+ (14.1 mg/g) that can be viewed using the Temkin and Freundlich models, respectively. At a pH value of 6.5 ± 0.1 and a temperature of 20 °C, EPS showed a greater affinity for Cu2+ than for Cd2+. The binding force between EPS and quartz sand was weak. The prior saturation of the sand media with EPS solution can significantly promote the migration of the Cu2+ and Cd2+ in sand columns by 8.8% and 32.1%, respectively. When treating both metals simultaneously, the migration of Cd2+ was found to be greater than that of Cu2+. This also demonstrated that EPS can promote the co-migration of Cu2+ and Cd2+ in saturated porous media.

Soil characteristics and microbial community response in rare earth mining areas in southern Jiangxi Province, China.

The microbial community and functional flora in rare earth mining areas are correlated, but the characteristics and metabolic pathways of pollutant in such mining areas are still poorly known. The heavy metals, rare earth elements, and microorganisms present after mining of rare earth mine sites were analyzed. After mining, all sampling sites exhibited low pH and low total organic carbon levels, accompanied by high iron and aluminum concentrations. The development of vegetation is closely related to the development of microorganisms. In the complex environment of rare earth mining areas, Proteobacteria exhibit an absolute competitive advantage. During mine environmental recovery, the relative abundances of Acidobacteria and Chloroflexi will increase markedly, and with further restoration the relative abundance of Firmicutes will gradually decrease. Many genera of bacteria related to the N cycle and heavy metal metabolism were detected in the study area, indicating the important metabolic pathways for ammonia nitrogen and heavy metals in rare earth mining areas. Bacterial genera that promote plant nitrogen fixation also occur in the area, further revealing the nitrogen cycle. This research is important for health assessment and recovery of rare earth mines.

Degradation mechanism of tris(2-chloroethyl) phosphate (TCEP) as an emerging contaminant in advanced oxidation processes: A DFT modelling approach.

As a typical toxic organophosphate and emerging contaminant, tris(2-chloroethyl) phosphate (TCEP) is resistant to conventional water treatment processes. Studies on advanced oxidation processes (AOPs) to degrade TCEP have received increasing attention, but the detailed mechanism is not yet fully understood. This study investigated the mechanistic details of TCEP degradation promoted by OH by using the density functional theory (DFT) method. Our results demonstrated that in the initial step, energy barriers of the hydrogen abstraction pathways were no more than 7 kcal/mol. Cleavage of the P-O or C-Cl bond was possible to occur, whilst the C-O or C-C cleavage had to overcome an energy barrier above 50 kcal/mol, which was too high for mild experimental conditions. The bond dissociation energy (BDE) combined with the distortion/interaction energy (DIE) analysis disclosed origin of the various reactivities of each site of TCEP. The systematic calculations on the transformation of products generated in the initial step showed remarkable exothermic property. The novel information at molecular level provides insight on how these products are generated and offers valuable theoretical guidance to help develop more effective AOPs to degrade TCEP or other emerging environmental contaminant.

Spectrophotometric Determination of p-Nitrophenol under ENP Interference.

Engineered nanoparticles (ENPs) have been widely developed in various fields in recent years, resulting in an increasing occurrence of nanoparticles in the natural environment. However, the tiny substances have created unexpected confusion in environmental sample testing due to the negative nanoeffect of ENPs. In this paper, a novel technique of spectrophotometric determination of p-nitrophenol (PNP) was developed under the interfering impact of nano-Fe(OH)3, widely distributed in the natural environment as a typical example of ENPs. Because of the strong absorption at the two characteristic peaks of PNP, namely, 317 nm and 400 nm, nano-Fe(OH)3 interfered with the colorimetric determination of PNP. Thus, the developed testing method, with HCl acidification at 60°C and ascorbic acid (AA) masking FeCl3, was proposed and successfully realized the accurate determination of PNP in water samples by ultraviolet spectrophotometry with 317 nm as the absorption wavelength. The final colorimetric system of 5% HCl, 10% CH3OH, and 1% ascorbic acid was confirmed by optimized batch experiments, and the optimum condition of acidification pretreatment was heating at 60°C for 20 min. Further results demonstrated that the proposed novel method had good accuracy and reproducibility even in high-salinity natural water bodies such as groundwater and surface water. The testing technique presented in this paper provided an interesting and useful tool for problem solving of PNP surveys under ENPs' interference and practically supported water quality assessment for a better environment.

Potential risk assessment of groundwater to address the agricultural and domestic challenges in Ordos Basin.

The safety of groundwater has been a great concern for irrigation and drinking purposes in recent decades due to the increasing impacts of anthropogenic activities. There are several standards to evaluate the groundwater quality for different utilization purposes. In this paper, 804 samples covering the entire Ordos Basin across five provinces were used to evaluate the irrigation suitability and human health risks. The results showed that the sequence of cationic concentration was Na+ > Ca2+ > Mg2+ > K+ > NH4 +, and the anion concentration was HCO3 - > SO4 2- > Cl- > NO3 - > F- > NO2 -. For drinking purposes, TDS, Na+, SO4 2-, F-, TH and NO3 - exceed seriously the regulated standard in the study area. For irrigating purposes, 80% of the water in the study area belongs to "good water" according to the evaluation of salinity and alkalinity. Saline water which is not suitable for irrigation accounts for about 9%. High health risks of fluoride ions are mainly observed to the samples representing the western part of the study area, while the health risks of nitrates spread throughout the study area. Health risk is not only related to location, but also varies with age, and it is found that children suffer more threats than adults.

Nanobiochar paper based electrochemical immunosensor for fast and ultrasensitive detection of microcystin-LR.

A portable, cheap and sensitive paper type electrochemical immunosensor was developed with conductive nanobiochar paper as the conductive layer and utilized for sensitive detection of microcystin-LR (MCLR) toxin in water. The paper immunosensor was constructed by coating of highly conductive and dispersible nanobiochar particle (nBC) and anti-MCLR antibody on the filter paper via dipping-drying method. The presence of MCLR could be specifically quantified amperometrically by the nBC-paper immunosensor with the response time of less than 5 min, and the lowest detection limit of 17 pM (0.017 µg/L) was achieved. Moreover, the proposed immunosensor exhibited high selectivity, reproducibility and storage stability, and was also used for environmental water detection with satisfactory recovery. The successful fabrication of low cost and ubiquitous biochar based paper type electrochemical immunosensing system would have significant value for the development of highly cost-effective electrochemical device.

Deposition and mobilization of viruses in unsaturated porous media: Roles of different interfaces and straining.

The vadose zone is the first natural layer preventing groundwater pollution. Understanding virus transport and retention in the vadose zone is necessary. The effects of different interfaces and mechanisms on virus transport and retention were investigated by studying Escherichia coli phage migration in laboratory-scale columns under unsaturated conditions. The E. coli phage was used as a model virus. Colloid filtration theory, extended Derjagin-Landau-Verwey-Overbeek theory and two-site kinetic deposition model were used to calculate fitted parameters and interaction energies to assess virus retention at different interfaces. The collector diameters and the size of E. coli phages in the influent and effluent were compared to assess the effect of straining. The results indicated that the roles of solid-water interfaces (SWIs) and air-water interfaces (AWIs) in retaining E. coli phages are strongly controlled by the moisture content and hydrochemical conditions. Decreasing the moisture content and increasing the ionic strength (IS) of the suspension increased E. coli phage retention. At suspension ISs of 0.01 or 0.03 M and various moisture contents, E. coli phages were mainly retained at the SWIs rather than AWIs. When the IS was increased to 0.06 M, the viruses were strongly retained by becoming attached to both SWIs and AWIs. The role of straining in virus retention could not be ignored. Viruses were retained more at the SWIs and less straining occurred under acidic conditions than under neutral or alkaline conditions. This was mainly because of the effects of the pH and IS on surface charges and the model virus particle size. This study has important implications for modeling and predicting virus transport in soil affected by rainfall, snowmelt, and human activities (e.g., irrigation and artificial groundwater recharging).

Sustainable Chromium (VI) Removal from Contaminated Groundwater Using Nano-Magnetite-Modified Biochar via Rapid Microwave Synthesis.

This study developed a nano-magnetite-modified biochar material (m-biochar) using a simple and rapid in situ synthesis method via microwave treatment, and systematically investigated the removal capability and mechanism of chromium (VI) by this m-biochar from contaminated groundwater. The m-biochar was fabricated from reed residues and magnetically modified by nano-Fe3O4. The results from scanning electron microscopy (SEM) and X-ray diffraction (XRD) characterisations confirmed the successful doping of nano-Fe3O4 on the biochar with an improved porous structure. The synthesised m-biochar exhibited significantly higher maximum adsorption capacity of 9.92 mg/g compared with that (8.03 mg/g) of the pristine biochar. The adsorption kinetics followed the pseudo-second-order model and the intraparticle diffusion model, which indicated that the overall adsorption rate of Cr(VI) was governed by the processes of chemical adsorption, liquid film diffusion and intramolecular diffusion. The increasing of the pH from 3 to 11 significantly affected the Cr(VI) adsorption, where the capabilities decreased from 9.92 mg/g to 0.435 mg/g and 8.03 mg/g to 0.095 mg/g for the m-biochar and pristine biochar, respectively. Moreover, the adsorption mechanisms of Cr(VI) by m-biochar were evaluated and confirmed to include the pathways of electrostatic adsorption, reduction and complexation. This study highlighted an effective synthesis method to prepare a superior Cr(VI) adsorbent, which could contribute to the effective remediation of heavy metal contaminations in the groundwater.