Structural evolution during inverse vulcanization.

Inverse vulcanization exploits S8 to synthesize polysulfides. However, evolution of products and its mechanism during inverse vulcanization remains elusive. Herein, inverse vulcanization curves are obtained to describe the inverse vulcanization process in terms of three stages: induction, curing and over-cure. The typical curves exhibit a moduli increment before declining or plateauing, reflecting the process of polysulfide network formation and loosing depending on monomers. For aromatic alkenes, in the over-cure, the crosslinked polysulfide evolves significantly into a sparse network with accelerated relaxation, due to the degradation of alkenyl moieties into thiocarbonyls. The inverse vulcanization product of olefins degrades slowly with fluctuated relaxation time and modulus because of the generation of thiophene moieties, while the inverse vulcanization curve of dicyclopentadiene has a plateau following curing stage. Confirmed by calculations, the mechanisms reveal the alkenyl groups react spontaneously into thiocarbonyls or thiophenes via similar sulfur-substituted alkenyl intermediates but with different energy barriers.

CsPbBr3 Perovskite Quantum Dots Encapsulated by a Polymer Matrix for Ultrasensitive Dynamic Imaging of Intracellular MicroRNA.

Herein, CsPbBr3 perovskite quantum dots (CPB PQDs)@poly(methyl methacrylate) (PMMA) (CPB@PMMA) nanospheres were used as energy donors with high Förster resonance energy transfer (FRET) efficiency and exceptional biocompatibility for ultrasensitive dynamic imaging of tiny amounts of microRNAs in living cells. Impressively, compared with traditional homogeneous single QDs as energy donors, CPB@PMMA obtained by encapsulating numerous CPB PQDs into PMMA as energy donors could not only significantly increase the efficiency of FRET via improving the local concentration of CPB PQDs but also distinctly avoid the problem of cytotoxicity caused by divulged heavy metal ions entering living cells. Most importantly, in the presence of target miRNA-21, DNA dendrimer-like nanostructures labeled with 6-carboxy-tetramethylrhodamine (TAMRA) were generated by the exposed tether interhybridization of the Y-shape structure, which could wrap around the surface of CPB@PMMA nanospheres to remarkably bridge the distance of FRET and increase the opportunity for effective energy transfer, resulting in excellent precision and accuracy for ultrasensitive and dynamic imaging of miRNAs. As proof of concept, the proposed strategy exhibited ultrahigh sensitivity with a detection limit of 45.3 aM and distinctly distinguished drug-irritative miRNA concentration abnormalities with living cells. Hence, the proposed enzyme-free CPB@PMMA biosensor provides convincing evidence for supplying accurate information, which could be expected to be a powerful tool for bioanalysis, diagnosis, and prognosis of human diseases.

In-situ direct seawater electrolysis using floating platform in ocean with uncontrollable wave motion.

Direct hydrogen production from inexhaustible seawater using abundant offshore wind power offers a promising pathway for achieving a sustainable energy industry and fuel economy. Various direct seawater electrolysis methods have been demonstrated to be effective at the laboratory scale. However, larger-scale in situ demonstrations that are completely free of corrosion and side reactions in fluctuating oceans are lacking. Here, fluctuating conditions of the ocean were considered for the first time, and seawater electrolysis in wave motion environment was achieved. We present the successful scaling of a floating seawater electrolysis system that employed wind power in Xinghua Bay and the integration of a 1.2 Nm3 h-1-scale pilot system. Stable electrolysis operation was achieved for over 240 h with an electrolytic energy consumption of 5 kWh Nm-3 H2 and a high purity (>99.9%) of hydrogen under fluctuating ocean conditions (0~0.9 m wave height, 0~15 m s-1 wind speed), which is comparable to that during onshore water electrolysis. The concentration of impurity ions in the electrolyte was low and stable over a long period of time under complex and changing scenarios. We identified the technological challenges and performances of the key system components and examined the future outlook for this emerging technology.

An all-natural, bioinspired, biodegradable electrical insulating composite based on lignocellulose and mica tailings.

The rapid development of modern electrical engineering puts forward urgent demand for high-performance electrical insulating materials. In this study, inspired by the layered structure of natural nacre, we present a novel biomimetic composite insulating film (referred to as M/C film) that is derived from agricultural waste corncobs and industrial waste mica tailings through a sol-gel-film transformation process. The novel insulating film has excellent tensile strength (94 MPa), high dielectric strength (68 kV mm-1), low dielectric loss, good heat resistance (T0 = 235 °C), and excellent UV shielding properties. Meanwhile, the M/C film presents environmental impacts much lower than those petrochemical-based plastic film as it can be 100 % recycled in a closed-loop recycling process and easily biodegraded in the environment (lignocellulose goes back to the carbon cycle and the mica return to the geological cycle). It is a potential alternative for petrochemical plastics and provides a possible way to utilize agricultural waste and mica tailings.

Ultrasensitive Fluorescence Detection and Imaging of MicroRNA in Cells Based on a Hyperbranched RCA-Assisted Multiposition SDR Signal Amplification Strategy.

Herein, an innovative fluorescent sensor was courageously empoldered for precise and ultrasensitive detection and imaging of target miRNA-21 through the agency of a dextrous target-motivated polymerization/nicking DNA nanomachineries based on a hyperbranched rolling circle amplification (HB-RCA)-assisted multiposition strand displacement reaction (SDR) signal amplification approach. Impressively, the ingenious technique not only realized target recycling via polymerization/nicking DNA nanomachineries but also involved HB-RCA amplification induced by the released transformation target as the repeated signal amplification. Most importantly, HB-RCA was firstly exploited to remarkably increase the local concentration and collision efficiency of the templates and primers, which could simultaneously generate multiple repeated DNA sequences as initiators to supply substantial banding positions for SDR, removing the massive fluorescence-resonance-energy-transfer (FRET) DNA duplexes from the repeated DNA sequences to remarkably avert the self-quenching of the fluorescence signal due to self-aggregation caused by the winding of the HB-RCA products, thereby leading to a conspicuously improved signal amplification multiplier. As proof of concept, an ingenious technique effectively and accurately distinguished target miRNA-21 even with a tiny change in cells compared to the conventional fluorescence in situ hybridization (FISH) approach. Moreover, the proposed fluorescent method apparently discriminated drug-manipulative miRNA expression level abnormities. Therefore, the proposed cascade nucleic acid amplification strategy could provide an epigamic avenue for ultrasensitive imaging of diverse biomarkers, which help researchers to better study the tumor mechanism, thereby unambiguously increasing cancer cure rates and reducing the risk of recurrence.

Highly Efficient Aggregation-Induced Electrochemiluminescence of Al(III)-Cbatpy Metal-Organic Gels Obtained by Ultrarapid Self-Assembly for a Biosensing Application.

Aggregation-induced electrochemiluminescence (AIECL) has attracted extensive interest due to the significant increase in ECL response by restricting free intramolecular rotation and torsion, but traditional AIECL emitters suffer from limited ECL efficiency, high cost, and complex synthetic steps, dramatically limiting their applications. Herein, novel Al(III)-Cbatpy metal-organic gels (Al(III)-Cbatpy-MOGs) with nanofiber morphology and ultrarapid coordination of Al3+ and 4'-carboxylic acid-2,2':6',2″-terpyridine (Cbatpy) are developed, which demonstrates an excellent AIECL enhancement behavior far beyond that reported in ECL supramolecular gels. In view of the strong affinity of N and O atoms in Cbatpy toward Al3+, Al(III)-Cbatpy-MOGs with high viscosity and stability can be assembled in one step within about 15 s, easily conquering the main predicaments of current AIECL emitters: complicated synthesis steps and poor film formation. Impressively, the ECL efficiency of Al(III)-Cbatpy-MOGs with superemission is about 20 times higher than that of individual Cbatpy molecules, which is attributed to the aggregation of the organic ligand Cbatpy restricting intramolecular rotation and torsion to reduce nonradiative relaxation. Furthermore, compared with traditional metal complexes, Al(III)-Cbatpy-MOGs show the benefits of remarkable biocompatibility and low cost without the involvement of any organic solvents, noble metals, and rare metals. As proof, a "signal-off" sensing platform based on an Al(III)-Cbatpy-MOGs/S2O82- system was constructed for the sensitive detection of dopamine (DA) with a low detection limit of 0.34 nM. This strategy provides a novel method to prepare cheap metal-organic gels as a highly efficient AIECL emitter, which is promising as a luminescent molecular device and biosensor for clinical diagnostic applications.

Freestanding Bipolar Membranes with an Electrospun Junction for High Current Density Water Splitting.

Freestanding bipolar membranes (BPMs) with an extended-area water splitting junction were fabricated utilizing electrospinning. The junction layer was composed of a mixed fiber mat that was made by concurrently electrospinning sulfonated poly(ether ether ketone) (SPEEK) and quaternized poly(phenylene oxide) (QPPO), with water splitting catalyst nanoparticles intermittently deposited between the fibers. The mat was sandwiched between solution cast SPEEK and QPPO films and hot-pressed to form a dense trilayer BPM with an extended-area junction of finite thickness, composed of QPPO nanofibers embedded in a SPEEK matrix with the catalyst nanoparticles interspaced between the two polymers. The composition, ion-exchange capacity, and catalyst type/loading in the junction were varied, and the water splitting characteristics of the membranes were assessed. The best BPMs fabricated in this work employed a graphene oxide catalyst and exhibited a low trans-membrane voltage drop of about 0.82 V at 1000 mA/cm2 in water splitting experiments with 0.5 M Na2SO4 and stable water splitting operation for 60 h at 800 mA/cm2.

Ultrasensitive Fluorescence Detection and Accurate Colocalization Visualization of Dual-miRNAs in Cancer Cells Based on the Conjugated Chain Reaction of Multifunctional Pentagon DNA Nanostructures.

Herein, a multifunctional pentagon DNA nanostructure (MPDN) was assembled by the hybridization of a circular DNA scaffold containing five different fragments with five diverse DNA oligonucleotides for simultaneous sensitive detection and accurate colocalization imaging of dual-miRNAs in cancer cells. Exactly, the MPDN could specifically and efficiently internalize into folate (FA) receptor-overexpressed cells via specific binding of FA and the FA receptor to distinguish cancer cells from normal cells and transform trace amounts of targets miRNA-21 and miRNA-155 into substantial FAM and Cy5-labeled DNA polymers as the signal probe to generate two remarkable fluorescence emissions, realizing simultaneously sensitive detection of dual-miRNAs. Impressively, compared with traditional small fragment DNA probes with high fluidity, the DNA copolymers with extremely low diffusivity kept it in the originally generated position to achieve the colocalization imaging of dual-miRNAs more accurately for revealing the spatial expression information of dual-miRNAs in tissues and cells. This strategy provided programmable tool to simultaneously detect and accurately colocate dual-miRNAs for understanding normal physiology and the tumor mechanism.

One-Step Digital Droplet Auto-Catalytic Nucleic Acid Amplification with High-Throughput Fluorescence Imaging and Droplet Tracking Computation.

Digital droplet technology has emerged as a powerful new tool for biomarker analysis. Temperature cycling, enzymes, and off-chip processes are, nevertheless, always required. Herein, we constructed a digital droplet auto-catalytic hairpin assembly (ddaCHA) microfluidic system to achieve digital quantification of single-molecule microRNA (miRNA). The designed continuous chip integrates droplet generation, incubation, and fluorescence imaging on the chip, avoiding the requirement for extra droplet re-collection and heating operations. Clearly, the digital readout was obtained by partitioning miRNA into many individual pL-sized small droplets in which the target molecule is either present ("positive") or absent ("negative"). Importantly, the suggested enzyme-free auto-catalytic hairpin assembly (aCHA) in droplets successfully mitigated the effects of the external environment and thermal cycling on droplets, and its reaction rate is significantly superior to that of traditional CHA. We got excellent sensitivity with a linear correlation from 1 pM to 10 nM and a detection limit of 0.34 pM in the fluorescence spectrum section, as well as high selectivity to other miRNAs. Furthermore, the minimum target concentration could be reduced to 10 fM based on the high-throughput tracking computation of fluorescent droplets with a self-developed Python script, and the fluorescence intensity distribution agreed well with the theoretical value, demonstrating that it is feasible to detect miRNA efficiently and accurately, which has great potential applications in clinical diagnostics and biochemical research.

Discrimination between Cancer Cells and DNA-Damaged Cells: Pre-miRNA Region Recognition Based on Hyperbranched Hybrid Chain Reaction Amplification for Simultaneous Sensitive Detection and Imaging of miRNA and Pre-miRNA.

Herein, a novel region recognition of precursor microRNA (Pre-miRNA) based on hyperbranched hybrid chain reaction (HB-HCR) amplification was constructed to effectively eliminate the interference of Pre-miRNA to the mature microRNA (miRNA) by establishing the linear mapping relation between the two fluorescence signals produced by the miRNA sequence in the Pre-miRNA and Pre-miRNA residues to first realize simultaneous sensitive detection of Pre-miRNA and miRNA as well as highly sensitive imaging of intracellular Pre-miRNA and miRNA, which solves one main challenge of in vitro tumor disease diagnostics: inaccurate detection of tumor-induced miRNA changes. Impressively, this strategy easily distinguishes cancer cells from normal cells and DNA-damaged cells by the difference in miRNA and Pre-miRNA expression, which provides an innovative approach for accurate clinical diagnosis of cancer and precise treatment of prognosis.

A target-initiated autocatalytic 3D DNA nanomachine for high-efficiency amplified detection of MicroRNA.

Considering the challenges of generating simple and efficient DNA (deoxyribonucleic acid) nanomachines for sensitive bioassays and the great potential of target-induced self-cycling catalytic systems, herein, a novel autocatalytic three-dimensional (3D) DNA nanomachine was constructed based on cross-catalytic hairpin assembly on gold nanoparticles (AuNPs) to generate self-powered efficient cyclic amplification. Typically, the DNA hairpins H1, H2, H3 and H4 were immobilized onto AuNPs first. In the presence of target microRNA-203a, the 3D DNA nanomachines were triggered to activate a series of CHA (catalytic hairpin assembly) reactions. Based on the rational design of the system, the products of the CHA 1 reaction were the trigger of the CHA 2 reaction, which could trigger the CHA 1 reaction in turn, generating an efficient self-powered CHA amplification strategy without adding fuel DNA strands or protein enzymes externally and producing high-efficiency fluorescence signal amplification. More importantly, the proposed autocatalytic 3D DNA nanomachines outperformed conventional 3D DNA nanomachines combined with the single-directional cyclic amplification strategy to maximize the amplification efficiency. This strategy not only achieves high-efficiency analysis of microRNAs (microribonucleic acids) in vitro and intracellularly but also provides a new pathway for highly processive DNA nanomachines, offering a new avenue for bioanalysis and early clinical diagnosis.

Acclimation to a broad range of nitrate strength on a euryhaline marine microalga Tetraselmis subcordiformis for photosynthetic nitrate removal and high-quality biomass production.

Industrial wastewaters usually possess a wide range of nitrate strength. Microalgae-based nitrate-rich wastewater treatment could realize nitrate recovery along with CO2 sequestration for sustainable biomass production, but the low tolerance of the microalgal strains to high-strength nitrate restricted the treatment process. The present study comprehensively evaluated a euryhaline marine microalga Tetraselmis subcordiformis for photosynthetic nitrate removal and biomass production in synthetic wastewater with a broad range of nitrate strength (0.24-7.0 g NO3--N/L). This alga could acclimate to high nitrate strength up to 3.5 g NO3--N/L (HN) without compromising biomass production. Nitrate could be completely removed within four days when low nitrate (0.24 g NO3--N/L, LN) was loaded. The maximum nitrate removal rate of 331 mg N/L/day and specific nitrate removal rate of 360 mg N/day/g cell was obtained under medium nitrate condition (1.8 g NO3--N/L, MN). High-nitrate stress under 7.0 g NO3--N/L (SHN) caused an increased light energy dissipation while decreased the density of photosystem II active reaction center, which partially protect the cells from photodamage and contributed to their acclimation to SHN. The algae also enhanced amino acid/fatty acid proportions essential for maintaining intracellular redox states to cope with the stress caused by LN or SHN. HN and SHN was in favor of protein accumulation and maintenance with enhanced proportion of essential amino acids, which entitled the algal biomass to be of high quality for animal feed applied in livestock graziery and aquaculture. LN facilitated productive starch and lipid accumulation with good quality for biofuels production. The nitrate removal rate and biomass productivity exceeded most of the microalgae reported in literature under similar conditions, which highlighted Tetraselmis subcordiformis as a potent strain for flexible nitrate-rich wastewater remediation coupled with fast CO2 bio-mitigation and high-quality biomass production for sustainable algal biorefinery.

Preparation of diameter-controlled free-standing MWCNT membranes and their application for dye adsorption.

The synthesis of multi-walled carbon nanotubes (MWCNTs) was carried out over different Ni-loaded metallic oxide catalyst nanoparticles and under different reduction times to control the outside diameter of the nanotubes. Moreover, high-purity, free-standing membranes were fabricated by a simple filtration of the as-grown MWCNTs. Furthermore, the dye-adsorption properties of the nanotubes depended on the diameter of the carbon nanotubes (CNTs). The adsorption isotherms and kinetics of anionic dyes could be described by Freundlich and pseudo-second-order models, respectively. Thermodynamic studies suggested that the adsorption processes were spontaneous and exothermic. This work provides new insights into the synthesis and application of MWCNTs with the selective adsorption properties of carbon-based materials for the removal of organic dyes.

Rapid self-disassembly of DNA diblock copolymer micelles via target induced hydrophilic-hydrophobic regulation for sensitive MiRNA detection.

In this work, a novel DNA nanostructure with a shorter assembly time and larger loading capacity was constructed using amphiphilic DNA-alkane group (Spacer C12)10 conjugates encapsulating plentiful fat-soluble fluorescent dyes into the hydrophobic core to form the DNA micelles, which could be rapidly self-disassembled via target induced hydrophilic-hydrophobic regulation to release fluorescent dyes from micelles to the organic phase, realizing the fast and sensitive detection of microRNA.

Aggregation-Induced Synergism by Hydrophobic-Driven Self-Assembly of Amphiphilic Oligonucleotides.

The evident contradiction between high local-concentration-based substrate reactivity and free-diffusion-based high reaction efficiency remains one of the important challenges in chemistry. Herein, we propose an efficient aggregation-induced synergism through the hydrophobic-driven self-assembly of amphiphilic oligonucleotides to generate high local concentration whereas retaining high reaction efficiency through hydrophobic-based aggregation, which is important for constructing efficient DNA nanomachines for ultrasensitive applications. MicroRNA-155, used as a model, triggered strand displacement amplification of the DNA monomers on the periphery of the 3D DNA nanomachine and generated an amplified fluorescent response for its sensitive assay. The local concentration of substrates was increased by a factor of at least 9.0×105 through hydrophobic-interaction-based self-assembly in comparison with the traditional homogeneous reaction system, achieving high local-concentration-based reactivity and free-diffusion-based enhanced reaction efficiency. As expected, the aggregation-induced synergism by hydrophobic-driven self-assembly of amphiphilic oligonucleotides created excellent properties to generate a 3D DNA nanomachine with potential as an assay for microRNA-155 in cells. Most importantly, this approach can be easily expanded for the bioassay of various biomarkers, such as nucleotides, proteins, and cells, offering a new avenue for simple and efficient applications in bioanalysis and clinical diagnosis.

A Janus 3D DNA nanomachine for simultaneous and sensitive fluorescence detection and imaging of dual microRNAs in cancer cells.

Herein, a Janus three-dimensional (3D) DNA nanomachine was constructed for the simultaneous and sensitive fluorescence detection and imaging of dual microRNAs (miRNAs) in cancer cells, which could effectively eliminate signal interference in a homogeneous nanoparticle-based 3D DNA nanostructure caused by the proximity of the two different signal probes to achieve accurate co-location in the same position of living cancer cells. In this system, the Janus nanoparticles were synthesized as the carrier for immobilizing two different oligonucleotides on two different functionalized hemispheres of the nanoparticles to form a Janus 3D DNA nanostructure, which could convert trace amounts of miRNA-21 and miRNA-155 targets into massive FAM and Cy5-labeled duplexes to induce two remarkable fluorescence emissions by the catalytic hairpin assembly (CHA) and 3D DNA walker cascade nucleic acid amplification strategy, realizing sensitive detection and imaging of miRNA targets in cancer cells. Impressively, in comparison with current miRNA imaging methods based on nanoparticle assemblies, the proposed strategy could efficiently eliminate "false positive" results obtained in single type miRNA detection and distinctly increase the immobilization concentration of two different signal probes using Janus nanoparticles as the carrier to further enhance fluorescence intensity, resulting in accurate co-location in the same position of living cells. Meanwhile, the proposed fluorescence imaging technology makes it possible to visualize low concentrations of miRNAs with tiny change associated with some cancers, which could significantly improve the accuracy and precision compared to those of the conventional fluorescence in situ hybridization (FISH) approach. Therefore, it could serve as persuasive evidence for supplying accurate information to better understand biological processes and investigate mechanisms of various biomolecules and subcellular organelles, resulting in the further validation of their function in tumor proliferation and differentiation. This strategy provided an innovative approach to design new generations of nanomachines with ultimate applications in bioanalysis and clinical diagnoses.

A dynamic 3D DNA nanostructure based on silicon-supported lipid bilayers: a highly efficient DNA nanomachine for rapid and sensitive sensing.

Herein, by anchoring cholesterol-labelled DNA probes to silicon-supported lipid bilayers via cholesterol-lipid interaction, a dynamic three-dimensional (3D) DNA nanostructure could be facilely assembled, which is applied as a microRNA (miRNA)-induced self-powered 3D DNA nanomachine with high movement efficiency. Once the self-powered 3D DNA nanomachine is triggered by target miRNA, it achieves autonomous operation without external addition of fuel DNA strands or protein enzymes. Impressively, the biocompatible lipid bilayers not only preserve the biological character of the DNA probes, but also improve the movement efficiency of the DNA nanomachine, which directly solves the key challenge of the steric barrier effect of traditional rigid surfaces (Au or silicon) for DNA probe diffusion. As a proof of concept, our proposed DNA nanomachine is successfully applied in rapid and sensitive detection of miRNAs, which gives a new idea for the construction of highly efficient DNA nanomachines for biosensing and clinic diagnosis.

A novel fluorescent assay for the ultrasensitive detection of miRNA-21 with the use of G-quadruplex structures as an immobilization material for a signal indicator.

In this work, a novel fluorescent assay was proposed for the ultrasensitive detection of microRNA-21 (miRNA-21) based on the efficient immobilization of protoporphyrin IX (PPIX) as signal indicators in massive G-quadruplex structures obtained by target recycling, three-dimensional DNA walker and a rolling circle amplification (RCA) coupled cascade nucleic acid amplification strategy.

Synthesis of hierarchical Co3O4@NiO core-shell nanotubes with a synergistic catalytic activity for peroxidase mimicking and colorimetric detection of dopamine.

Fabrication of core-shell nanostructured catalyst is a promising way for tuning its catalytic performance due to the highly active interface and rich redox properties. In this work, hierarchical Co3O4@NiO core-shell nanotubes are fabricated by the deposition of NiO shells via a chemical bath treatment using electrospun Co-C composite nanofibers as templates, followed by a calcination process in air. The as-prepared Co3O4@NiO core-shell nanotubes exhibit a uniform and novel hollow structure with Co3O4 nanoparticles attached to the inner wall of NiO nanotubes and excellent catalytic activity toward the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) in the presence of H2O2. Due to the synergistic effect, the peroxidase-like activity of the Co3O4@NiO core-shell nanotubes is much higher than that of individual Co3O4 and NiO components. Owing to the superior peroxidase-like activity, a simple and rapid colorimetric approach for the detection of dopamine with a detection limit of 1.21µM and excellent selectivity has been developed. It is anticipated that the prepared Co3O4@NiO core-shell nanotubes are promising materials applied for biomedical analysis and environmental monitoring.

Oxidase-mimicking activity of perovskite LaMnO3+δ nanofibers and their application for colorimetric sensing.

Nanomaterials with ABX3-type perovskite structure have attracted considerable and increasing attention due to their unique physical and chemical properties as well as promising applications in various fields. In this work, we have developed a simple electrospinning followed by a calcination process to prepare ABO3-type perovskite LaMnO3+δ nanofibers as efficient oxidase mimics for the detection of l-cysteine. The oxidase-like catalytic activity of the prepared LaMnO3+δ nanofibers is heavily dependent on the calcination temperature which results in different sizes of the LaMnO3+δ crystals and their crystallinity, and the maximum activity is achieved at the calcination temperature of 700 °C. Based on the high oxidase-like catalytic activity of the as-prepared perovskite LaMnO3+δ nanofibers, a simple and accurate colorimetric detection method towards l-cysteine has been developed. The detection limit is as low as 109.8 nM and an excellent selectivity for l-cysteine detection with common substances in human blood as interferents is also achieved. In addition, the LaMnO3+δ nanofibers can be retained as a monolithic membrane after the calcination process, making them an oxidase mimic for on-demand colorimetric sensing. This work reveals the promising prospects for the perovskite LaMnO3+δ materials in biosensing, medical diagnosis, food safety and environmental monitoring.