Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 1 de 1
Filter
Add more filters










Database
Language
Publication year range
1.
Med Oncol ; 32(1): 361, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25432696

ABSTRACT

MicroRNAs (miRNAs) have been suggested to play critical roles in tumorigenesis as well as in the development of therapies for the treatment of cancers. However, the tumor-associated miRNAs in gastric cancers remain poorly understood. Here, we report on miR-542-3p in gastric cancers, which has been widely studied in other cancers as a tumor suppressor. Real-time quantitative PCR analysis demonstrated that miR-542-3p was significantly down-regulated in gastric cancer tissues (p < 0.0001) and cell lines (p < 0.001). Overexpression of miR-542-3p significantly inhibited cell growth of gastric cancer cells both in vitro (p < 0.01) and in vivo (p < 0.01). Notably, overexpression of miR-542-3p apparently reduced the protein expression of astrocyte-elevated gene-1 (AEG-1) (p < 0.01). The dual-luciferase reporter assay validated that miR-542-3p directly bound the 3'-untranslated region (UTR) of AEG-1, which could be abolished by mutation of the predicted miR-542-3p binding site. Furthermore, overexpression of miR-542-3p markedly inhibited the activation of oncogenic signaling pathways including the Akt, ß-catenin and nuclear factor-κB pathways. Additionally, overexpression of AEG-1 without the 3'-UTR partially reversed the cell growth arrest induced by miR-542-3p overexpression in gastric cancer cells (p < 0.05). Taken together, these data suggest that miR-542-3p might function as a tumor suppressor in gastric cancer, potentially by targeting the oncogene AEG-1, implying a potential role for miR-542-3p in the development of therapeutic methods for gastric cancer.


Subject(s)
Cell Adhesion Molecules/biosynthesis , Gene Expression Regulation, Neoplastic/genetics , MicroRNAs/genetics , Stomach Neoplasms/genetics , Animals , Blotting, Western , Cell Adhesion Molecules/genetics , Cell Proliferation/genetics , Female , Genes, Tumor Suppressor , Heterografts , Humans , Male , Membrane Proteins , Mice , Mice, Inbred BALB C , Mice, Nude , RNA-Binding Proteins , Real-Time Polymerase Chain Reaction , Stomach Neoplasms/pathology
SELECTION OF CITATIONS
SEARCH DETAIL
...