The study of dental status through determination of the degree of preservation of paleoanthropological material.

OBJECTIVE: Aim: To introduce a simple classification system for the degree of preservation and quality of the dentoalveolar apparatus into scientific circulation to further investigate dental diseases in ancestral populations.. PATIENTS AND METHODS: Materials and Methods: The sample analyzed consisted of the remains of 499 individuals from the human populations that existed in the territories of Ukraine from the Copper Age to the Bronze Age. Teeth and jaws were examined macroscopically under bright light; dental changes were evaluated by probing. Dental radiographic examinations were performed using retroalveolar and panoramic X-ray films in adult individuals. RESULTS: Results: The authors divide the dentoalveolar apparatus of fossil and subfossil skeletons into ten levels for further medical investigation of dental diseases. These levels depend on the preservation degree and changes in jaws and teeth. CONCLUSION: Conclusions: The study of bone and tooth preservation, determination of skeletal sex, and age characteristics is a separate section and stage in reconstructing the physical type of individuals and the health status of a community. Studying the taphonomic features of odontologic anthropological material should be a mandatory prerequisite preceding its analysis in paleopathology. The condition of the dentoalveolar system can be classified into ten levels for paleopathological conclusions. This classification will be helpful to doctors specializing in forensic medicine and anthropologists.

PERIODONTAL DISEASE AND SALIVARY OXIDATION STRESS IN CHILDREN WITH LYMPHOGRANULOMATOSIS.

OBJECTIVE: The aim: To investigate the impact of lymphogranulomatosis (LGM) and periodontal disease on salivary lipid peroxidation and enzymatic antioxidants` levels in children. PATIENTS AND METHODS: Materials and methods: 45 children aged 6-16 years with LGM were examined before hematologic therapy (group LGM 1), after therapy (group LGM 2), and at the remission (group LGM 3). The control group included 70 healthy children. Periodontal state of children, saliva thiobarbituric acid reacting substances (TBARS), superoxide dismutase (SOD) and catalase were examined. RESULTS: Results: 6-11 years old children from LGM 1 group showed a higher frequency of periodontal disease (50,0%), as well as 12-15 year olds (80,8%) compared to healthy children (17,4% and 42,8% accordingly, p<0,05). TBARS levels were higher in LGM 1-3 groups of children with periodontal disease (9,79, 12,3 and 12,6 umol/l, р<0,01) compared to counterparts without it (8,01, 10,1 and 11,6 umol/l, р<0,01) and healthy children with periodontal disease (7,9 umol/l, р<0,01). SOD activity was higher in LGM 1-3 groups of children with periodontal disease (-0,075, -0,086, -0,074 units) compared to children without it (-0,048, -0,059, -0,04 units, р<0,01) and healthy children with periodontal disease (-0,04 units, р<0,01). Catalase activity was lower in LGM 1-3 groups of children with periodontal disease (6,72, 5,2 and 6,7 units) compared to counterparts without it (7,3, 3,7 and 4,7 units, р<0,01) and healthy children with periodontal disease (7,1 units, р<0,01). CONCLUSION: Conclusions: Children with periodontal disease related to LGM had higher TBARS levels, SOD activity and lower catalase activity in saliva. Both LGM and periodontal disease altered lipid peroxidation and antioxidant protection in saliva of children.

NEUTROPHIL ACTIVITIES IN ADOLESCENTS WITH TYPE I DIABETES MELLITUS DEPENDING ON PERIODONTAL STATE.

OBJECTIVE: The aim: To estimate the neutrophil activities in adolescents with type 1 diabetes mellitus (T1DM) depending on periodontal state. PATIENTS AND METHODS: Materials and methods: A total of 93 individuals aged 12-16 years, including 62 T1DM patients and 31 healthy (H) controls, were included. Both groups were categorized into subgroups depending on their periodontal state. Phagocytic activity of neutrophils (PAN) the index of neutrophil activation (INA), and the percent of formazan-active neutrophils were evaluated using the spontaneous and the induced nitroblue tetrazolium (sNBT and iNBT) tests into oral rinses. RESULTS: Results: PAN was significantly higher in the healthy (H) controls with gingivitis compared with the individuals with gingival health (p < 0.0001). This parameter decreased significantly in the T1DM subjects, especially with periodontitis, compared with the H controls (p < 0.0001). The percent of formazan-active neutrophils and INA in the sNBT test increased in the T1DM patients with gingival health and continued to raise as periodontal state of adolescents with T1DM worsened (p<0.0001). The parameters of the iNBT test in the T1DM adolescents decreased with the periodontal disease development (p < 0.0001) that may demonstrate that superoxide production exhausts in diabetes, especially associated with periodontal disease. CONCLUSION: Conclusions: The sNBT test in studied adolescents showed that both periodontal disease and T1DM increase the rate of activated neutrophils (p<0,05).