ABSTRACT
The occurrence of hepatocellular carcinoma (HCC) is a multistep development process through precancerous lesions. A precancerous lesion of HCC is classified into hepatocyte dysplasia at the cytological level and dysplastic nodules at the histological level, and the corresponding lesion subtypes have different risks of canceration. Pathology is the "gold standard" for the diagnosis of early stage HCC and its precancerous lesions. However, it also faces many difficulties and challenges, such as the accumulation of experience in the pathological diagnosis, the understanding and grasp of key points of histopathological diagnosis and differential diagnosis, the combination application of immune and molecular diagnostic markers, and many others. This article briefly discusses the key points of pathological features and differential diagnosis of precancerous lesions of HCC.
ABSTRACT
In recent years, there's a new theory of lipid condensation or accumulation being the etiology for obstruction of qi in the chest result in heart disease. The theory believes that thick lipid exists in meridians. If one takes too much fatty diet, there will be lipid condensation or accumulation, resulting in disturbance of qi transformation in meridians,. Scholars explore the relationship of preventive treatment of disease by traditional Chinese medicine with lipid condensation or accumulation theory. This enriches the etiology of TCM.
ABSTRACT
Stroke is one of the leading causes of unnatural death and disability. No effective therapy is available. Recombinant human granulocyte colony-stimulating factor (rhG-CSF), as a mobilizing agent for bone marrow stem cells, can promote stem cell mobilization, homing to brain after cerebral ischemia. In the present study, the administration of G-CSF significantly increased number of CD34(+) cells in the marginal zone of the infarction. Rats receiving G-CSF had higher survival rate and lower infarction volume. Neurological behavior was improved, and the expression of fibronectin in the ischemic brain was increased, as compared to rats treated with vehicle. To mimic the ischemia-reperfusion injury in experimental animals, we employed hippocampal slice cultures that were first treated with oxygen and glucose deprivation (OGD) and then with oxygen-glucose resupply, finding that fibronectin significantly increased the neurite outgrowth of OGD hippocampal slices, upregulated the expression of Bcl-2 protein, and ameliorated the ultrastructure damage of OGD hippocampal slices.
Subject(s)
Fibronectins/physiology , Granulocyte Colony-Stimulating Factor/pharmacology , Ischemic Attack, Transient/prevention & control , Neuroprotective Agents , Animals , Antigens, CD34/metabolism , Antimetabolites/pharmacology , Behavior, Animal/drug effects , Brain/pathology , Brain/ultrastructure , Bromodeoxyuridine/pharmacology , Cell Death/physiology , Cell Survival/drug effects , Cerebral Infarction/pathology , Fibronectins/biosynthesis , Genes, bcl-2 , Glial Fibrillary Acidic Protein/metabolism , Glucose/deficiency , Hippocampus/pathology , Hippocampus/ultrastructure , Hypoxia, Brain/pathology , Immunohistochemistry , Ischemic Attack, Transient/pathology , Male , Microscopy, Electron , Neurons/pathology , Neurons/physiology , Neurons/ultrastructure , Rats , Rats, Sprague-Dawley , Stem Cells/physiology , Stem Cells/ultrastructure , Survival Analysis , Up-Regulation/physiologyABSTRACT
Objective To observe the effect of Fuzheng Hunao capsule on TNF-? and HSP70 protein expression of myocardial tissues in rats with intracerebral hemorrhage.Methods Seventy two rats were randomly divided into 4 groups:sham,model,Fuzheng Hunao and Angong Niuhuang.The protein expression of TNF-? and HSP70 in rat myocardial tissues were detected by the immunohistochemistiy method,and the integrated optical density (IOD) was measured with image analysis.Result The IOD of TNF-? and HSP70 in model group was higher than that in sham group.The IOD of TNF-? in Fuzheng Hunao and Angong Niuhuang group was lower than that in model group.While the IOD of HSP70 was higher than that in model group.Conclusion Inflammatory response attend the myocardial damage.Fuzheng Hunao capsule could protect the myocardial tissues by inhibiting the protein expression of TNF-? and increasing that of HSP70.
ABSTRACT
Objective To observe the effect of Fuzhenghunao capsule on NE of the locus ceruleus and myocardial tissues in rats with intracerebral hemorrhage. Methods 72 rats were randomly divided into 4 groups:sham,model,Fuzhenghunao and Angongniuhuang. The content of NE were tested by the high performance liquid phase-electrochemical method at 6 h,24 h and 72 h of post-operation. Result NE was obviously increased in sham group,and decreased in two treatment groups. Conclusion Fuzhenghunao capsule could be used to inhibit the activation of locus ceruleus noradrenalin system and protect myocardial tissues by regulating NE content.
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AIM:To explore clinical effect of Zhongfeng Fangzhiling Granule(Radix Pseudostellariae,Radix Polygoni Multiflori praeparata cum succo glycines sotae,Hirudo,etc.) on acute cerebral thrombosis.METHODS:72 patients were randomly divided into 2 groups.The treatment group was treated with Zhongfeng Fangzhiling Granule,the control group was treated with Zhilong Xuetong Capsule.The treatment effects between two groups were compared.RESULTS:The effect in treatment group was superior to that in control group.CONCLUSION:Zhongfeng Fangzhiling Granule has significiant therapeutic effect on patients with acute cerebral thrombosis,and the preparation method is simple,so it will be a prospective herb granule.
