ABSTRACT
The ability to develop cutaneous response to Mantoux, phytohemagglutinin (PHA) and dinitrochlorobenzene (DNCB) was studied in patients with carcinomas in the digestive tract, with particular emphasis on cancer of the colon, in patients with benign pathology and in a control group. The patients with a malignant disease in the digestive tract, considered as a group, showed a remarkable decrease of responses to Mantoux (31%), to PHA (21%) and to DNCB (58%) compared to the control group where incidence amounted to 61%, 73% and 100% respectively. The depression in the immune cellular response is more remarkable yet in colon carcinoma (Mantoux 11%, PHA 0%, DNCB 11%) all of which suggest that the impairment of the immune response depends on the tumor localization. An increase of unresponsiveness was also observed within the group with a benign pathology although the deterioration is lower than that observed in the malignant group. The patients with adenocarcinoma of the colon received BCG immunotherapy and the changes in cellular and humoral response were studied in them. Although the improving mechanism does not seem to affect simultaneously the various expressions of cell mediated immunity, a stimulating effect in the response was observed in what refers to recall antigen, inflammatory reaction and sensitization to DNCB. These changes ranked Mantoux greater than DNCB greater than PHA. There was a direct correlation between skin test reactivity to at least one antigen and the patient survival, but is not the case with the changes observed in the immunoglobulins level. The significance of these changes in relation to the possible anti-tumor effect is discussed.
Subject(s)
Adenocarcinoma/immunology , BCG Vaccine/pharmacology , Colonic Neoplasms/immunology , Immunocompetence/drug effects , Adult , Aged , Dinitrochlorobenzene/immunology , Female , Humans , Immunity, Cellular , Immunoglobulins/analysis , Male , Middle Aged , Skin TestsABSTRACT
The ability to develop cutaneous response to Mantoux, phytohemagglutinin (PHA) and dinitrochlorobenzene (DNCB) was studied in patients with carcinomas in the digestive tract, with particular emphasis on cancer of the colon, in patients with benign pathology and in a control group. The patients with a malignant disease in the digestive tract, considered as a group, showed a remarkable decrease of responses to Mantoux (31
), to PHA (21
) and to DNCB (58
) compared to the control group where incidence amounted to 61
, 73
and 100
respectively. The depression in the immune cellular response is more remarkable yet in colon carcinoma (Mantoux 11
, PHA 0
, DNCB 11
) all of which suggest that the impairment of the immune response depends on the tumor localization. An increase of unresponsiveness was also observed within the group with a benign pathology although the deterioration is lower than that observed in the malignant group. The patients with adenocarcinoma of the colon received BCG immunotherapy and the changes in cellular and humoral response were studied in them. Although the improving mechanism does not seem to affect simultaneously the various expressions of cell mediated immunity, a stimulating effect in the response was observed in what refers to recall antigen, inflammatory reaction and sensitization to DNCB. These changes ranked Mantoux greater than DNCB greater than PHA. There was a direct correlation between skin test reactivity to at least one antigen and the patient survival, but is not the case with the changes observed in the immunoglobulins level. The significance of these changes in relation to the possible anti-tumor effect is discussed.
ABSTRACT
The ability to develop cutaneous response to Mantoux, phytohemagglutinin (PHA) and dinitrochlorobenzene (DNCB) was studied in patients with carcinomas in the digestive tract, with particular emphasis on cancer of the colon, in patients with benign pathology and in a control group. The patients with a malignant disease in the digestive tract, considered as a group, showed a remarkable decrease of responses to Mantoux (31
), to PHA (21
) and to DNCB (58
) compared to the control group where incidence amounted to 61
respectively. The depression in the immune cellular response is more remarkable yet in colon carcinoma (Mantoux 11
) all of which suggest that the impairment of the immune response depends on the tumor localization. An increase of unresponsiveness was also observed within the group with a benign pathology although the deterioration is lower than that observed in the malignant group. The patients with adenocarcinoma of the colon received BCG immunotherapy and the changes in cellular and humoral response were studied in them. Although the improving mechanism does not seem to affect simultaneously the various expressions of cell mediated immunity, a stimulating effect in the response was observed in what refers to recall antigen, inflammatory reaction and sensitization to DNCB. These changes ranked Mantoux greater than DNCB greater than PHA. There was a direct correlation between skin test reactivity to at least one antigen and the patient survival, but is not the case with the changes observed in the immunoglobulins level. The significance of these changes in relation to the possible anti-tumor effect is discussed.
ABSTRACT
In vitro metabolism of (3H)-testosterone from male accessory gland homogenates from autoimmunized and normal rabbits was studied at different times of incubation. Results indicated that 5 alpha - androstane-3 alpha, 17 beta-diol was the main metabolite formed in both cases, though the presence of the 3 beta-isomer cannot be excluded. On autoimmunized rabbits with small histological alteration, transformation of the precursor (3H)-testosterone was significantly greater (40 min: P less than 0.01; 60 min: P less than 0.05). This led to a higher yield of 5 alpha-androstane-3 alpha, 17 beta-diol at both incubation times, being significant only at 40 min (P less than 0.02). The 4-androstene-3,17-dione also increased as compared with the normal group. In autoimmunized rabbits with a greater histological alteration, the bioconversion of (3H)-testosterone decreased for both incubation times, being significant only for the 40 min (P less than 0.05). A decreased interconversion to 4-androstene-3,17-dione was also observed, being significant only for 40 min (P less than 0.05). These results suggest that in an early stage of autoimmunization there might be a transient stimulation of enzyme activities in the sexual accessory glands. In another moment of the phenomena a more severe histological lesion with infiltration of male accessory glands was present. At the same time, decrease in the enzymatic activities could be noticed.
Subject(s)
Autoantibodies , Genitalia, Male/metabolism , Testosterone/metabolism , 3-Hydroxysteroid Dehydrogenases/biosynthesis , Androstane-3,17-diol/biosynthesis , Animals , In Vitro Techniques , Male , Prostate/metabolism , Prostate/pathology , Rabbits , Seminal Vesicles/metabolism , Seminal Vesicles/pathologyABSTRACT
In vitro metabolism of (3H)-testosterone from male accessory gland homogenates from autoimmunized and normal rabbits was studied at different times of incubation. Results indicated that 5 alpha - androstane-3 alpha, 17 beta-diol was the main metabolite formed in both cases, though the presence of the 3 beta-isomer cannot be excluded. On autoimmunized rabbits with small histological alteration, transformation of the precursor (3H)-testosterone was significantly greater (40 min: P less than 0.01; 60 min: P less than 0.05). This led to a higher yield of 5 alpha-androstane-3 alpha, 17 beta-diol at both incubation times, being significant only at 40 min (P less than 0.02). The 4-androstene-3,17-dione also increased as compared with the normal group. In autoimmunized rabbits with a greater histological alteration, the bioconversion of (3H)-testosterone decreased for both incubation times, being significant only for the 40 min (P less than 0.05). A decreased interconversion to 4-androstene-3,17-dione was also observed, being significant only for 40 min (P less than 0.05). These results suggest that in an early stage of autoimmunization there might be a transient stimulation of enzyme activities in the sexual accessory glands. In another moment of the phenomena a more severe histological lesion with infiltration of male accessory glands was present. At the same time, decrease in the enzymatic activities could be noticed.
ABSTRACT
In vitro metabolism of (3H)-testosterone from male accessory gland homogenates from autoimmunized and normal rabbits was studied at different times of incubation. Results indicated that 5 alpha - androstane-3 alpha, 17 beta-diol was the main metabolite formed in both cases, though the presence of the 3 beta-isomer cannot be excluded. On autoimmunized rabbits with small histological alteration, transformation of the precursor (3H)-testosterone was significantly greater (40 min: P less than 0.01; 60 min: P less than 0.05). This led to a higher yield of 5 alpha-androstane-3 alpha, 17 beta-diol at both incubation times, being significant only at 40 min (P less than 0.02). The 4-androstene-3,17-dione also increased as compared with the normal group. In autoimmunized rabbits with a greater histological alteration, the bioconversion of (3H)-testosterone decreased for both incubation times, being significant only for the 40 min (P less than 0.05). A decreased interconversion to 4-androstene-3,17-dione was also observed, being significant only for 40 min (P less than 0.05). These results suggest that in an early stage of autoimmunization there might be a transient stimulation of enzyme activities in the sexual accessory glands. In another moment of the phenomena a more severe histological lesion with infiltration of male accessory glands was present. At the same time, decrease in the enzymatic activities could be noticed.
ABSTRACT
Immunodiffusion and immuno-electrophoresis techniques were used to detect rabies precipitable antigens. The passive haemagglutination test with group "O" human red cells treated with tannic acid, was used as control test. The study was done with brain suspensions of rabies virus infected mice (CVS strain). Mouse and horse rabies antisera were used. The analysis of the results of both immunochemical assays showed the presence of two specific antigenic fractions of rabies virus. The same two fractions were detected when the antigens were heated. Experiments with brain extracts centrifuged at high speed, suggested that the antigenic fractions are of the "soluble" type.
Subject(s)
Antigens, Viral/analysis , Rabies virus/immunology , Animals , Antigens, Viral/immunology , Hemagglutination Tests , Humans , Immune Sera , Immunodiffusion , Immunoelectrophoresis , MiceABSTRACT
Immunodiffusion and immuno-electrophoresis techniques were used to detect rabies precipitable antigens. The passive haemagglutination test with group [quot ]O[quot ] human red cells treated with tannic acid, was used as control test. The study was done with brain suspensions of rabies virus infected mice (CVS strain). Mouse and horse rabies antisera were used. The analysis of the results of both immunochemical assays showed the presence of two specific antigenic fractions of rabies virus. The same two fractions were detected when the antigens were heated. Experiments with brain extracts centrifuged at high speed, suggested that the antigenic fractions are of the [quot ]soluble[quot ] type.
ABSTRACT
Immunodiffusion and immuno-electrophoresis techniques were used to detect rabies precipitable antigens. The passive haemagglutination test with group [quot ]O[quot ] human red cells treated with tannic acid, was used as control test. The study was done with brain suspensions of rabies virus infected mice (CVS strain). Mouse and horse rabies antisera were used. The analysis of the results of both immunochemical assays showed the presence of two specific antigenic fractions of rabies virus. The same two fractions were detected when the antigens were heated. Experiments with brain extracts centrifuged at high speed, suggested that the antigenic fractions are of the [quot ]soluble[quot ] type.
Subject(s)
Agammaglobulinemia/immunology , gamma-Globulins/analysis , Adolescent , Adult , Agammaglobulinemia/diagnosis , Humans , Male , Serologic TestsABSTRACT
The aim of the present report is to study comparatively the biosynthesis of testosterone in normal and autoimmune rabbit testes, with different degrees of histological lesion, by in vitro double tracer incubation experiments. For this purpose (3H)-pregnenolone and (14C)-progesterone were used as precursors in the presence of testicular homogenates from both groups of animals. In the autoimmune animals, an impairment in the biotransformation of the precursors leading to a lesser synthesis of testosterone was demonstrated. An accumulation of dehydroepiandrosterone, 17 alpha-hydroxyprogesterone and androstenedione was frequently found. It was also shown that the metabolic impairment seemed to be produced at an earlier stage than the histological modifications. On the other hand, a correlation between the degree of testicular damage and the intensity of metabolic impairment could not be demonstrated conclusively.
Subject(s)
Autoantibodies/immunology , Testicular Diseases/metabolism , Testis/metabolism , Testosterone/biosynthesis , Animals , Male , Metabolic Diseases/etiology , Pregnenolone/pharmacology , Progesterone/pharmacology , Rabbits , Testicular Diseases/pathology , Testis/immunologyABSTRACT
The aim of the present report is to study comparatively the biosynthesis of testosterone in normal and autoimmune rabbit testes, with different degrees of histological lesion, by in vitro double tracer incubation experiments. For this purpose (3H)-pregnenolone and (14C)-progesterone were used as precursors in the presence of testicular homogenates from both groups of animals. In the autoimmune animals, an impairment in the biotransformation of the precursors leading to a lesser synthesis of testosterone was demonstrated. An accumulation of dehydroepiandrosterone, 17 alpha-hydroxyprogesterone and androstenedione was frequently found. It was also shown that the metabolic impairment seemed to be produced at an earlier stage than the histological modifications. On the other hand, a correlation between the degree of testicular damage and the intensity of metabolic impairment could not be demonstrated conclusively.
ABSTRACT
The aim of the present report is to study comparatively the biosynthesis of testosterone in normal and autoimmune rabbit testes, with different degrees of histological lesion, by in vitro double tracer incubation experiments. For this purpose (3H)-pregnenolone and (14C)-progesterone were used as precursors in the presence of testicular homogenates from both groups of animals. In the autoimmune animals, an impairment in the biotransformation of the precursors leading to a lesser synthesis of testosterone was demonstrated. An accumulation of dehydroepiandrosterone, 17 alpha-hydroxyprogesterone and androstenedione was frequently found. It was also shown that the metabolic impairment seemed to be produced at an earlier stage than the histological modifications. On the other hand, a correlation between the degree of testicular damage and the intensity of metabolic impairment could not be demonstrated conclusively.