ABSTRACT
【Objective】 To investigate the clinical characteristics, long-term follow-up rate, level and control rate of low-density lipoprotein cholesterol (LDL-C) in patients with atherosclerotic cardiovascular disease (ASCVD) aged ≥75 years who underwent percutaneous coronary intervention (PCI) during hospitalization. 【Methods】 We selected ASCVD patients aged ≥75 years with PCI from January 2016 to December 2020 in The First Affiliated Hospital of Xi’an Jiaotong University, collected the baseline data of the patients and the follow-up of 1 month, 3 months, 6 months and 12 months after discharge by HIS system, and analyzed their LDL-C and control rate at each follow-up. 【Results】 A total of 1 129 patients were enrolled in this study, aged 78 (ranging from 75 to 89) years. Among them 72.1% were male; myocardial infarction was the main type of ASCVD (71.5% ); hypertension was the most common risk factor, accounting for 85.2% (717/842), followed by diabetes, 58.6% (493/842); 74.6% met the ultra-high risk criteria of the 2020 Chinese Expert Consensus on Lipid Management in Ultra-High Risk ASCVD Patients, and the LDL-C control rate was only 8.1% . The four routine follow-up rates of 1 129 elderly ASCVD patients were 49.5%, 24.1%, 17.1%, and 24.6%, respectively. The detection rates of LDL-C during follow-up were 26.3%, 5.3%, 10.4%, and 13.8%, respectively. LDL-C control rates in ultra-high risk ASCVD were 59.4%, 45.1%, 37.1%, and 17.6%, respectively, while LDL-C control rates in non-ultra-high risk ASCVD patients were 67.3%, 55.6%, 47.4%, and 44.0%, respectively. 【Conclusion】 The elderly patients with ASCVD-PCI were mainly ultra-high risk patients. The routine follow-up rate and the LDL-C compliance rate during follow-up were low and showed a downward trend.
ABSTRACT
Granulosa cells (GCs) are essential somatic cells in the ovary and play an important role in folliculogenesis. Brain-derived neurotropic factor (BDNF) and the TGF-ß pathway have been identified as a critical hormone and signalling pathway, respectively, in GCs. In this study, we found that a conserved microRNA family that includes miR-10a and miR-10b repressed proliferation and induced apoptosis in human, mouse, and rat GCs (hGCs, mGCs and rGCs, respectively). Moreover, essential hormones and growth factors in the follicle, such as FSH, FGF9 and some ligands in the TGF-ß pathway (TGFß1, Activin A, BMP4 and BMP15), inhibited miR-10a and miR-10b expression in GCs. In contrast, the miR-10 family suppressed many key genes in the TGF-ß pathway, suggesting a negative feedback loop between the miR-10 family and the TGF-ß pathway in GCs. By using bioinformatics approaches, RNA-seq, qPCR, FISH, immunofluorescence, Western blot and luciferase reporter assays, BDNF was identified as a direct target of the miR-10 family in GCs. Additionally, reintroduction of BDNF rescued the effects of miR-10a and miR-10b in GCs. Collectively, miR-10a and miR-10b repressed GC development during folliculogenesis by repressing BDNF and the TGF-ß pathway. These effects by the miR-10 family on GCs are conserved among different species.
Subject(s)
Apoptosis/genetics , Conserved Sequence , Granulosa Cells/cytology , Granulosa Cells/metabolism , MicroRNAs/metabolism , Animals , Apoptosis/drug effects , Brain-Derived Neurotrophic Factor/metabolism , Cell Line , Cell Proliferation/drug effects , Cell Proliferation/genetics , Female , Gene Expression Regulation/drug effects , Granulosa Cells/drug effects , Hormones/pharmacology , Humans , Intercellular Signaling Peptides and Proteins/pharmacology , Mice , MicroRNAs/genetics , Rats , Species Specificity , Transcriptome/geneticsABSTRACT
PURPOSE: To ensure the correct interpretation of the results of quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) from ovarian tissue cryopreserved by vitrification, it is critical to normalize expression levels to a reference gene with stable messenger RNA (mRNA) expression in the vitrified/warmed ovarian tissue. The aim of this work was to identify suitable reference genes for qRT-PCR analysis during ovarian cryopreservation by vitrification. METHODS: GeNorm, NormFinder, comparative Delta-CT, and BestKeeper were used to analyze the expression and stability of the 14 reference genes GAPDH, ABL1, ACTB, CDKN1A, GPER, GUSB, HPRT1, HSP90AB1, IPO8, PPIA, RPL4, RPL30, TBP, and UPAR. RESULTS: Our results indicated that ACTB and RPL4 were relatively stable reference genes in vitrified/warmed ovaries.
Subject(s)
Cryopreservation/methods , Ovary/physiology , Real-Time Polymerase Chain Reaction/methods , Actins/genetics , Animals , Female , Gene Expression Profiling/methods , Mice, Inbred C57BL , Real-Time Polymerase Chain Reaction/standards , Reference Standards , Ribosomal Proteins/genetics , VitrificationABSTRACT
To strongly promote the activity of reading classics among medical students, Chongqing University of Medical Sciences has made a sampling survey on the state of reading and their literature knowledge among the medical students. The survey shows that the medical students agree to the necessity and probability of reading classics, that they also show some obvious preferences for interest in reading and that there is a huge difference in hierarchy,grade and sex with regard to the grasp of literature, history, philosophy, arts and technology for medical students. The outcome of this survey will undoubtedly make a great revelation on how to carry out the activity of reading classics.
