Correlation between expression of LRP16, Ki67 and EGFR and breast cancer clinical pathologic factors and prognosis.

OBJECTIVE: To investigate the expression of leukemia-related protein 16 (LRP16), proliferating cell nuclear antigen K-67 (Ki67) and epidermal growth factor receptor-1 (EGFR-1) in breast cancer tissue and to explore the correlation between the expression of those proteins and breast cancer clinical pathologic factors and prognosis. PATIENTS AND METHODS: The expressions of LRP16, Ki67 and EGFR in breast cancer tissues of 86 cases were detected by the immunohistochemical method and the correlations between the expression of LRP16, Ki67 and EGFR and clinical pathologic factors and prognosis were investigated. RESULTS: Positive expression rates of LRP16, Ki67 and EGFR in breast cancer tissue were 52.3%, 70.9% and 16.3%, respectively. There was no statistical difference in the expression of RP16, Ki67 and EGFR between different age groups (p>0.05). The expression of LRP16 was correlated with clinical stage, histological grade, tumor size and lymphatic metastasis (p<0.05); the expression of Ki67 was correlated with clinical stage, histological grade, tumor size and lymphatic metastasis (p<0.05); the expression of EGFR was correlated with histological grade (p<0.05). Comparison of postoperative local recurrence and metastasis time between LRP 16 positive group and negative group showed statistically significant difference (p<0.05); comparison of postoperative local recurrence and metastasis time between Ki67 positive group and negative group also showed statistically significant difference (p<0.05); comparison of postoperative local recurrence and metastasis time between EGFR positive group and negative group showed no statistically significant difference (p<0.05). CONCLUSIONS: Detection of expression levels of LPR16, Ki67 and EGFR in breast cancer tissue improves the understanding of biological behaviors of breast cancer, which in turn provide clinical guidance in diagnosis, treatment and prognosis assessment.

Frequency-dependent electromechanics of aqueous liquids: electrowetting and dielectrophoresis.

Electrowetting on dielectric and dielectrophoretic electromechanical mechanisms dominate microfluidic actuation in the low- and high-frequency limits, respectively. The frequency-dependent relationship between these two mechanisms has been clarified by the Maxwell stress tensor and a simple RC circuit model. In this paper, we report extensive height-of-rise measurements obtained with vertical, parallel, dielectrically coated electrodes to test this relationship using deionized water and solutions containing sugar and salt. For DC and AC (20 Hz to 20 kHz) voltage magnitudes up to approximately 150 V-rms, the data are highly reproducible and, within experimental error, consistent with the square-law predictions of the model. Eventually as voltage is increased, a saturation phenomenon is observed which exhibits a weak dependence on frequency and is probably correlated to contact angle saturation.