ABSTRACT
[This corrects the article DOI: 10.1155/2019/7930396.].
ABSTRACT
Background: Imbalanced oxidative stress and antioxidant defense are involved in airway remodeling in asthma. It has been demonstrated that Tetrandrine has a potent role in antioxidant defense in rheumatoid arthritis and hypertension. However, the correlation between Tetrandrine and oxidative stress in asthma is utterly blurry. This study aimed to investigate the role of Tetrandrine on oxidative stress-mediated airway remolding. Materials and Methods: Chronic asthma was established by ovalbumin (OVA) administration in male Wistar rats. Histopathology was determined by HE staining. Immunofluorescence was employed to detect the expression of α-SMA and Nrf-2. Level of oxidative stress and matrix metalloproteinases were examined by ELISA kits. Cell viability and cell cycle of primary airway smooth muscle cells (ASMCs) were evaluated by CCK8 and flow cytometry, respectively. Signal molecules were detected using western blot. Results: Tetrandrine effectively impairs OVA-induced airway inflammatory and airway remodeling by inhibiting the expression of CysLT1 and CysLTR1. The increase of oxidative stress and subsequent enhancement of MMP9 and TGF-ß1 expression were rescued by the administration of Tetrandrine in the rat model of asthma. In in vitro experiments, Tetrandrine markedly suppressed TGF-ß1-evoked cell viability and cell cycle promotion of ASMCs in a dose-dependent manner. Furthermore, Tetrandrine promoted Nrf-2 nuclear transcription and activated its downstream HO-1 in vivo and in vitro. Conclusion: Tetrandrine attenuates airway inflammatory and airway remodeling in rat model of asthma and TGF-ß1-induced cell proliferation of ASMCs by regulating oxidative stress in primary ASMCs, suggesting that Tetrandrine possibly is an effective candidate therapy for asthma.
Subject(s)
Airway Remodeling/drug effects , Asthma/drug therapy , Benzylisoquinolines/therapeutic use , Immunosuppressive Agents/therapeutic use , Animals , Asthma/complications , Asthma/metabolism , Benzylisoquinolines/pharmacology , Chronic Disease , Disease Models, Animal , Drug Evaluation, Preclinical , Heme Oxygenase-1/metabolism , Immunosuppressive Agents/pharmacology , Male , Matrix Metalloproteinases/metabolism , Membrane Proteins/metabolism , Myocytes, Smooth Muscle/drug effects , NF-E2-Related Factor 2/metabolism , Oxidative Stress/drug effects , Phytotherapy , Plant Extracts/pharmacology , Plant Extracts/therapeutic use , Random Allocation , Rats, Wistar , Receptors, Leukotriene/metabolismABSTRACT
OBJECTIVES: In this study, a cocktail of probe drugs was used to assess whether lentinan could influence the activities of rat enzymes CYP3A4, CYP2D6, CYP1A2, CYP2C19, and CYP2C9 in vivo. MATERIALS AND METHODS: Fourteen days after intraperitoneal injection of lentinan, rats were given an oral dose of a cocktail solution containing phenacetin, tolbutamide, omeprazole, metoprolol, and midazolam. Then, we obtained blood in specific durations for the determination of plasma concentration of the probe drugs using UPLC-MS/MS. We also evaluated the pharmacokinetic parameters using the DAS 2.0 software. RESULTS: We found that various concentrations of lentinan increased the activity of rat CYP1A2, CYP3A4, CYP2D6, and CYP2C19 but not CYP2C9. CONCLUSION: These findings suggest that clinical application of lentinan combination with CYP3A4, CYP1A2, CYP2C19, or CYP2D6 should be given careful consideration as this may lead to herb-drug interactions and hence treatment failure.
ABSTRACT
OBJECTIVE: To study the effect of triptergium wilfordii polyglycosidium on the content of Th1 and Th2 in the treatment child patients of rcurrent nephrotic syndrome. METHOD: Patients were randomized into treatment group and health group. Sixty-one patients in treatment group were treated with triptergium wilfordii polyglycosidium 1 mg x kg(-1) x d(-1) orally tid for 12 weeks. However, patients in health group was not treated with any drugs. Twelve weeks constituted one course of treatment and the content of IL-12, IL-2, TNF-alpha, IL-13, IL-6, IL-4 in peripheral blood was measured before and behind therapy. RESULT: In treatment group, the content of serum cytokines behind therapy was significantly lower than that before therapy, except for IL-12. CONCLUSION: Triptergium wilfordii polyglycosidium could reduce the cytokine level (except for IL-12) of Th1 and Th2, which could lead a therapeutic effect of in the child patients of RNS.
Subject(s)
Drugs, Chinese Herbal/pharmacology , Glycosides/pharmacology , Nephrotic Syndrome/drug therapy , Nephrotic Syndrome/metabolism , Th1 Cells/drug effects , Th1 Cells/metabolism , Th2 Cells/drug effects , Th2 Cells/metabolism , Tripterygium/chemistry , Child , Female , Humans , Interleukin-12/metabolism , Interleukin-13/metabolism , Interleukin-2/metabolism , Interleukin-4/metabolism , Male , Tumor Necrosis Factor-alpha/metabolismABSTRACT
OBJECTIVE: To explore the effect of Chinese materia medica on immune intervention of infantile recurrent respiratory tract infection. METHOD: Thirty-one children of recurrent respiratory tract infection were randomly divided into two groups: therapy group was treated with oral Chinese materia medica (b. i. d), control group was only treated with oral carboxymethyl liquor (< 4 years, 3 mL; 4-7 years, 5 mL; > 7 years, 7 mL, t. i. d). The change of IL-12,TNF-alpha, IL-6, IL-13, IL-6 and IL-4 in different time were observed and analyzes. RESULT: Compared with the control group, the level of IL-12 and IL-2 was significantly increased after treatment of oral Chinese materia medica (P < 0.01), however, the level of TNF-alpha, IL-13, IL-4, and IL-6 was decreased after treatment (P < 0.01). During one years follow-up study, the frequency of respiratory infection every year of therapy group was significantly decreased than that of control group. CONCLUSION: Chinese materia medica could prevent infantile recurrent respiratory tract infection effectively, increase humoral immunity function and ensure normal growth in children.
