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1.
Comput Biol Med ; 176: 108498, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38744011

ABSTRACT

With advancements in science and technology, the depth of human research on COVID-19 is increasing, making the investigation of medical images a focal point. Image segmentation, a crucial step preceding image processing, holds significance in the realm of medical image analysis. Traditional threshold image segmentation proves to be less efficient, posing challenges in selecting an appropriate threshold value. In response to these issues, this paper introduces Inner-based multi-strategy particle swarm optimization (IPSOsono) for conducting numerical experiments and enhancing threshold image segmentation in COVID-19 medical images. A novel dynamic oscillatory weight, derived from the PSO variant for single-objective numerical optimization (PSOsono) is incorporated. Simultaneously, the historical optimal positions of individuals in the particle swarm undergo random updates, diminishing the likelihood of algorithm stagnation and local optima. Moreover, an inner selection learning mechanism is proposed in the update of optimal positions, dynamically refining the global optimal solution. In the CEC 2013 benchmark test, PSOsono demonstrates a certain advantage in optimization capability compared to algorithms proposed in recent years, proving the effectiveness and feasibility of PSOsono. In the Minimum Cross Entropy threshold segmentation experiments for COVID-19, PSOsono exhibits a more prominent segmentation capability compared to other algorithms, showing good generalization across 6 CT images and further validating the practicality of the algorithm.


Subject(s)
Algorithms , COVID-19 , SARS-CoV-2 , COVID-19/diagnostic imaging , Humans , Image Processing, Computer-Assisted/methods , Machine Learning
2.
Arch Virol ; 169(6): 126, 2024 May 16.
Article in English | MEDLINE | ID: mdl-38753067

ABSTRACT

A novel mitovirus was identified in Fusarium oxysporum f. sp. melonis strain T-SD3 and designated as "Fusarium oxysporum mitovirus 3" (FoMV3). The virus was isolated from diseased muskmelon plants with the typical symptom of fusarium wilt. The complete genome of FoMV3 is 2269 nt in length with a predicted AU content of 61.40% and contains a single open reading frame (ORF) using the fungal mitochondrial genetic code. The ORF was predicted to encode a polypeptide of 679 amino acids (aa) containing a conserved RNA-dependent RNA polymerase (RdRp) domain with a molecular mass of 77.39 kDa, which contains six conserved motifs with the highly conserved GDD tripeptide in motif IV. The 5'-untranslated region (UTR) and 3'-UTR of FoMV3 were predicted to fold into stem-loop structures. BLASTp analysis revealed that the RdRp of FoMV3 shared the highest aa sequence identity (83.85%) with that of Fusarium asiaticum mitovirus 5 (FaMV5, a member of the family Mitoviridae) infecting F. asiaticum, the causal agent of wheat fusarium head blight. Phylogenetic analysis further suggested that FoMV3 is a new member of the genus Unuamitovirus within the family Mitoviridae. This is the first report of a new mitovirus associated with F. oxysporum f. sp. melonis.


Subject(s)
Fungal Viruses , Fusarium , Genome, Viral , Open Reading Frames , Phylogeny , Plant Diseases , Fusarium/virology , Plant Diseases/microbiology , Plant Diseases/virology , Fungal Viruses/genetics , Fungal Viruses/isolation & purification , Fungal Viruses/classification , RNA Viruses/genetics , RNA Viruses/isolation & purification , RNA Viruses/classification , Whole Genome Sequencing , RNA, Viral/genetics , RNA-Dependent RNA Polymerase/genetics , Viral Proteins/genetics , Cucumis melo/virology , Cucumis melo/microbiology , Amino Acid Sequence , 5' Untranslated Regions , 3' Untranslated Regions , Base Sequence
3.
J Hazard Mater ; 459: 132293, 2023 10 05.
Article in English | MEDLINE | ID: mdl-37597391

ABSTRACT

Microbial electron flow (MEF) is produced from microbial degradation of organic compounds. Regulating MEF to promote organic pollutants biodegradation such as naphthalene (Nap) is a potential way but remains a lack of theoretical basis. Here, we regulated MEF by adding electron acceptor NO3- to achieve 2.6 times increase of Nap biodegradation with cyclodextrin as co-metabolism carbon source. With the NO3- addition, the genes inhibited by Nap of electron generation significantly up-regulated. Especially, key genes ubiD and nahD for anaerobic Nap degradation significantly up-regulated respectively 3.7 times and 6.7 times. Moreover, the ability of electron transfer in MEF was also improved consistent with 7.2 times increase of electron transfer system (ETS) activity. Furthermore, total 60 metagenome-assembled genomes (MAGs) were reconstructed through the metagenomic sequencing data with assembly and binning strategies. Interestingly, it was also first found that the Klebsiella MAG. SDU (Shandong University) 14 had the ability of simultaneous Nap biodegradation and denitrification. Our results firstly offered an effective method of regulating MEF to promote polycyclic aromatic hydrocarbons (PAHs) degradation and simultaneous methanogenesis.


Subject(s)
Electrons , Nitrates , Humans , Anaerobiosis , Organic Chemicals , Naphthalenes , Microbial Interactions , Oxidants
4.
Small ; 19(38): e2302386, 2023 Sep.
Article in English | MEDLINE | ID: mdl-37196415

ABSTRACT

Sluggish redox kinetics and shuttle effect of polysulfides hinder the extensive application of the lithium-sulfur batteries (LSBs). Herein a functional heterostructure of boron nitride (BN) and MXene with an alternately layered structure (BN@MXene) is designed as separator interlayer. High efficiency Li+ transmission, uniform lithium deposition, strong adsorption, and efficient catalytic conversion activities of lithium polysulfides (LiPSs) realized by this heterostructure are confirmed by experiments and theoretical calculations. The alternately layered structure provides unblocked ion transmission channels and abundant active sites to accelerate the polysulfides redox kinetics with reduced energy barriers of oxidation and reduction reactions. As a result, the LSBs deliver an initial discharge capacity of up to 1273.9 mAh g-1 at 0.2 °C and a low decay of 0.058% per cycle in long-term cycling up to 700 cycles at 1 °C. This work provides an effective designing strategy to accelerate the polysulfides redox kinetics for advanced Li-S electrochemical system.

6.
J Oral Pathol Med ; 52(5): 448-455, 2023 May.
Article in English | MEDLINE | ID: mdl-36935549

ABSTRACT

BACKGROUND: Oral lichen planus (OLP) is a mucocutaneous inflammatory disease affecting 1% general population. Tripartite motif-containing protein 21 (TRIM21) shows a significant role in OLP. This study aimed to explore the function and mechanism of TRIM21 in T cells of OLP. METHODS: Differential gene expression profile in OLP versus healthy controls (HCs) was constructed by RNA sequencing. Protein expression level and infiltration sites of TRIM21 in OLP were detected by immunoblot, immunohistochemistry, and immunofluorescence. Expression of proinflammatory cytokines and chemokines including IL-6, TNF-α, ICAM1, CXCL1, CXCL8, CXCL9, and CXCL11 in CD3+ TRIM21hi T cells were measured by quantitative real-time polymerase chain reaction analysis. Downstream pathways and substrates of TRIM21 were explored by immunoblot and immunoprecipitation. Whether TRIM21 ubiquitination its substrate and ubiquitination form were tested by ubiquitination assay in vitro. RESULTS: Compared with HCs, TRIM21 exhibited a higher level in OLP, which expressed mainly in CD3+ T lymphocytes in OLP tissues. Overexpressed TRIM21 enhanced the expression of IL-6, TNF-α, CXCL1, CXCL8, CXCL9, and CXCL11 in CD3+ T cell line through ubiquitinating nuclear factor-κB (NF-κB) via a lysine 63 (K63) linkage, which eventually activating NF-κB signaling pathway. CONCLUSIONS: In OLP, TRIM21 promoted inflammation through ubiquitylating NF-κB and activating NF-κB signaling pathway.


Subject(s)
Lichen Planus, Oral , NF-kappa B , Humans , Inflammation , Interleukin-6/metabolism , Keratinocytes/metabolism , Lichen Planus, Oral/pathology , NF-kappa B/metabolism , T-Lymphocytes/metabolism , Tumor Necrosis Factor-alpha/metabolism
7.
J Environ Manage ; 329: 117010, 2023 Mar 01.
Article in English | MEDLINE | ID: mdl-36603323

ABSTRACT

Phenanthrene (PHE) as a typical polycyclic aromatic hydrocarbon (PAH) is prevalent and harmful to organisms in petroleum-polluted sites. The effects of PHE concentration levels on performance, microbial community and functions in methanogenic system were comprehensively investigated by an operation of UASB reactor (198 days) and a series of batch tests. The results found that PHE was prone to accumulate in reactor by sludge adsorption (Final concentration = 12.53 mg/g TS Sludge), which posed significant influences on methanogenic system. The removal of chemical oxygen demand (COD), NH4+-N and volatile fatty acids (VFAs) in reactor were reduced with PHE accumulation. Meanwhile, microbes with higher ATPase secrete more EPS activity to self-protect against PHE toxicity. Sequencing analysis showed that PHE interfered significantly diversity and structure of microbial community. For bacteria, PHE was toxic to Bacteroidetes and Latescibacteria, while syntrophs (f_Syntrophaceae, Syntrophorhabdus, etc.) involved in VFAs oxidation and aromatic organics degradation were tolerant of PHE stress. For archaea, acetoclastic methanogens (Methanosaeta) abundance was continuously diminished by 45.1% under long-term PHE exposure. Further functions analysis suggested that microbial community accelerated amino acid metabolism, energy metabolism and xenobiotics biodegradation & metabolism to satisfy physiological demanding under PHE stress. Combining batch tests of methanogenic metabolism proved that acetoclastic methanogenesis was negatively affected by PHE due to inhibition of functional enzymes (acetate kinase, phosphate acetyltransferase, etc.) expression. These findings may provide the basis for enhancing bioremediation of PAH pollution in anaerobic environment.


Subject(s)
Euryarchaeota , Polycyclic Aromatic Hydrocarbons , Sewage/chemistry , Biodegradation, Environmental , Adsorption , Archaea/genetics , Bacteria/metabolism , Euryarchaeota/metabolism
8.
Oral Dis ; 2023 Jan 27.
Article in English | MEDLINE | ID: mdl-36705399

ABSTRACT

BACKGROUND: Photodynamic therapy (PDT) relies on the light activation of a photosensitizers to generate reactive oxygen species such as singlet oxygen, but its effect on cancer therapy is limited dramatically by hypoxia in the tumor microenvironment. OBJECTIVES: To determine the potential of a nano-photosensitizer loaded salvianolic acid B (SalB) and 5-aminolevulinic acid (ALA) for enhancing the efficacy of PDT in oral squamous cell carcinoma Cal27 cells and leukoplakia Leuk1 cells. RESULTS: Singlet oxygen sensor green (SOSG) assay showed that nano-SalB-ALA generated higher levels of singlet oxygen, compared to nano-SalB and nano-ALA. Cellular uptake assay showed that nano-SalB-ALA effectively absorbed by Leuk1 cells. Importantly, cell counting kit-8 and flow cytometry revealed that PDT with nano-SalB-ALA effectively inhibited the viability and induced the apoptosis of Cal27 and Leuk1 cells, respectively. Moreover, the tumor xenograft study revealed that PDT with nano-SalB-ALA had a stronger inhibitory effect on tumor growth of nude mice, compared to control groups. CONCLUSIONS: The novel photosensitizer nano-SalB-ALA remarkably enhanced the efficacy of PDT by improving singlet oxygen production, inhibiting cell proliferation, promoting cell apoptosis, and suppressing tumor growth. These suggest PDT with nano-SalB-ALA could be a clinically significant and potent treatment for oral cancer and leukoplakia.

9.
Stem Cells Int ; 2022: 5817684, 2022.
Article in English | MEDLINE | ID: mdl-36483681

ABSTRACT

Cancer-derived small extracellular vesicles (sEVs) are emerging as crucial mediators of intercellular communication between cancer cells and M2-tumor-associated macrophages (M2-TAMs) via transferring lncRNAs. We previously reported that miR-134 blocks the expression of its targeting protein LAMC2 via the PI3K/AKT pathway and inhibits cancer stem cell (CSC) migration and invasion in oral squamous cell carcinoma (OSCC). This study hypothesize that OSCC-CSC-derived small extracellular vesicles (OSCC-CSC-sEVs) transfer a ceRNA of miR-134 and consequently promote M2 macrophage polarization by targeting LAMC2 via the PI3K/AKT pathway through in vitro and in vivo experiment methods. The results showed that sEVs derived from CD133+CD44+ OSCC cells promoted M2 polarization of macrophages by detecting several M2 macrophage markers (CD163, IL-10, Arg-1, and CD206+CD11b+). Mechanistically, we revealed that the lncRNA UCA1, by binding to miR-134, modulated the PI3K/AKT pathway in macrophages via targeting LAMC2. Importantly, OSCC-CSC-sEV transfer of UCA1, by targeting LAMC2, promoted M2 macrophage polarization and inhibited CD4+ T-cell proliferation and IFN-γ production in vitro and in vivo. Functionally, we demonstrated that M2-TAMs, by transferring exosomal UCA and consequently targeting LAMC2, enhanced cell migration and invasion of OSCC in vitro and the tumorigenicity of OSCC xenograft in nude mice. In conclusion, our results indicated that OSCC-CSC-sEV transfer of UCA1 promotes M2 macrophage polarization via a LAMC2-mediated PI3K/AKT axis, thus facilitating tumor progression and immunosuppression. Our findings provide a new understanding of OSCC-CSC molecular mechanisms and suggest a potential therapeutic strategy for OSCC through targeting CSC-sEVs and M2-TAMs.

10.
Front Nutr ; 9: 1017072, 2022.
Article in English | MEDLINE | ID: mdl-36245495

ABSTRACT

Emerging research has shown that polyunsaturated fatty acids (PUFAs) benefit human health and exert anti-cancer effects. However, there is little understanding of the specific mechanisms by which PUFAs regulate the cells of the ovarian granulosa tumor. In the current study, we investigate the effects and the possible mechanisms of PUFAs on human ovarian tumor cells development. KGN cells were treated with omega-3. Small interfering (siRNA) and specific activator were used to knock down and overexpress gene expression in KGN cells. The protein content levels were analyzed by Western blot. Cell viability, proliferation and apoptosis assay were performed to examine the cellular development. And the level of glucose uptake in KGN cells were assessed by 2-DG measurement. The results showed that omega-3 treatment reduced cell viability, proliferation and increased cell apoptosis. Further studies showed that omega-3 also reduced GLUT1/4 protein content and cellular glucose uptake. Subsequent knockdown and overexpression of OCT4 using Oct4 siRNA and O4I2 (OCT4 activator) showed that OCT4 was involved in the regulations of omega-3 on GLUT1/4 expression and cell development. Our data demonstrate that omega-3 inhibits cellular development by down-regulating GLUT1/4 expression and glucose uptake in KGN cells, which are mediated through OCT4.

11.
ACS Omega ; 7(42): 37286-37292, 2022 Oct 25.
Article in English | MEDLINE | ID: mdl-36312369

ABSTRACT

The effect of adding protein on the decomposition behavior of lignin in Japanese cedar under supercritical methanol conditions (270 °C/27 MPa) was studied. The Klason method was used to detect the lignin content in the insoluble residue following to a 30 min treatment. Adding either an animal (bovine serum albumin) or plant (soy) protein enhanced delignification from 50 to 65% of the lignin-based wt %. This result was attributed to enhanced lignin depolymerization owing to inhibited lignin recondensation and/or the suppressed formation of polysaccharide-derived char via reactions between the protein and polysaccharides. Although the solubilization of lignin was promoted and the yield of lignin-derived low-molecular-weight compounds increased, the selectivity of major monomers such as coniferyl alcohol (CA) and γ-methylated CA decreased. The addition of proteins has a substantial impact on the decomposition behavior of cell wall components under supercritical methanol conditions. This information provides insights into the use of protein-rich lignocelluloses.

12.
J Environ Manage ; 312: 114934, 2022 Jun 15.
Article in English | MEDLINE | ID: mdl-35339793

ABSTRACT

This study evaluated the inhibitory effect and mitigation strategy of dodecyl dimethyl benzyl ammonium chloride (DDBAC) suppression on anaerobic digestion. With the 12 h-suppression, only 16.64% of anaerobes were alive, and acetotrophic methanogens were significantly inhibited. As for batch test, DDBAC suppression significantly prolonged the start-up of systems and decreased the biogas production. In cellulose semi-continuous digestion process, the DDBAC suppression induced volatile fatty acids accumulation and pH decrease. However, the biochar amended reactor effectively mitigated the DDBAC suppression and achieved 370.5 mL/d·g-chemical-oxygen-demand biogas production. Moreover, 17.8% more protein in extracellular polymeric substances was secreted as the bio-barrier to defense the DDBAC suppression. Furthermore, microbial analysis showed that biochar addition selectively enriched directed interspecies electron transfer (DIET) participant bacteria (Anaerolineaceae and Syntrophomonas) and methanogens (Methanosaeta and Methanobacterium). Meanwhile, the potential metabolic pathway analysis showed that the abundance of amino acids and energy metabolism were increased 28% and 8%, respectively. The abundance of encoding enzyme related to hydrogenotrophic and acetotrophic methanogenesis enriched 1.88 times and 1.48 times, respectively. These results showed the performance and mechanisms involved in DIET establishment with ethanol stimulation biochar addition.


Subject(s)
Ammonium Compounds , Cellulose , Ammonium Chloride , Anaerobiosis , Biofuels , Bioreactors , Charcoal , Humans , Methane , Sewage
13.
Lasers Surg Med ; 54(5): 682-687, 2022 07.
Article in English | MEDLINE | ID: mdl-35253237

ABSTRACT

OBJECTIVES: This study aimed to review the results of oral leucoplakia (OL) using ablative fractional laser-assisted photodynamic therapy (AFL-PDT) and to further evaluate the risk factors for recurrence and malignant transformation. MATERIALS AND METHODS: Forty-eight patients diagnosed with OL using histopathology were enrolled in this study. All patients received one session of AFL-PDT. Therapeutic efficacy was evaluated 1 month posttreatment. Follow-up was scheduled every 3 months in the first year and every 6 months thereafter. RESULTS: An overall positive response rate of 87.5% (42/48) was achieved, including 62.5% (30/48) complete responses and 25.0% (12/48) partial responses. During the 3-year follow-up period, the recurrence and malignant transformation rates were 37.5% (18/48) and 8.3% (4/48), respectively. Lesions on gingiva/palate seemed to be associated with recurrence (p < 0.001; odds ratio [OR]: 1.64, 95% confidence interval [CI]: 1.13-2.37). The severity of epithelial dysplasia (p = 0.02; OR: 2.93, 95% CI: 1.96-4.42) and recurrence (p = 0.016; OR: 3.14, 95% CI: 2.04-4.84) were associated with a predisposition to malignant transformation. CONCLUSIONS: AFL-PDT is an effective management of OL, but requires close follow-up. OL lesions on the gingiva/palate are predisposed to recurrence. OLs that recur with moderate/severe epithelial dysplasia have a higher risk of transforming into oral squamous cell carcinoma.


Subject(s)
Carcinoma, Squamous Cell , Lasers, Solid-State , Mouth Neoplasms , Photochemotherapy , Carcinoma, Squamous Cell/drug therapy , Humans , Lasers, Solid-State/therapeutic use , Leukoplakia, Oral/drug therapy , Leukoplakia, Oral/etiology , Mouth Neoplasms/drug therapy , Photochemotherapy/methods , Photosensitizing Agents/therapeutic use , Retrospective Studies , Treatment Outcome
14.
Taiwan J Obstet Gynecol ; 61(1): 174-179, 2022 Jan.
Article in English | MEDLINE | ID: mdl-35181034

ABSTRACT

OBJECTIVE: Beckwith-Wiedemann syndrome (BWS) is a rare imprinting gene disorder. Maternal CDKN1C mutation comprises 5% of etiologies of BWS. There is no successful report of preventing BWS by preimplantation genetic testing for monogenic disease (PGT-M) in the literature. Is PGT-M applicable for preventing BWS ? CASE REPORT: This 39-year-old woman conceived naturally and delivered a boy who was diagnosed of BWS. The genetic testing of her son revealed CDKN1C gene mutation, and of the mother showed a carrier of the same mutation. She underwent controlled ovarian stimulation, oocyte pickup, and intracytoplasmic sperm injection. Trophectoderm biopsies were performed and samples were checked for PGT. Two wild-type and euploid embryos were thawed and transferred. One intrauterine pregnancy was achieved. The patient delivered a healthy female baby at 37 weeks of gestation. CONCLUSION: In this case, we first report a successful pregnancy with a wild-type CDKN1C gene baby achieved by PGT-M.


Subject(s)
Beckwith-Wiedemann Syndrome/diagnosis , Cyclin-Dependent Kinase Inhibitor p57/genetics , Preimplantation Diagnosis , Sperm Injections, Intracytoplasmic , Adult , Beckwith-Wiedemann Syndrome/genetics , Female , Genetic Linkage , Genetic Testing , Genomic Imprinting , Humans , Male , Mutation , Pregnancy , Pregnancy Outcome
15.
J Colloid Interface Sci ; 613: 636-643, 2022 May.
Article in English | MEDLINE | ID: mdl-35065437

ABSTRACT

In order to fundamentally suppress the shuttle effect, N2 Plasma & Al2O3 magnetron sputtered separators (Al2O3@N-PP) are proposed for lithium-sulfur batteries (LSBs). Such a dual-functional polysulfides (LiPSs) barrier separator greatly inhibits the shuttle effect from the perspective of physical and chemical interaction. Physically, the inherently electronegative amorphous Al2O3 first achieves the repulsion of LiPSs to the sulfur cathode through the electrostatic repulsive effect, effectively preventing a large amount of soluble LiPSs from accumulating at the separator. At the same time, the Al2O3 film seals the shuttle channel of LiPSs to a certain extent. Chemically, N2 plasma-doped N heteroatoms form a lithium bond with Li+ in LiPSs to achieve the first step chemical adsorption and anchoring of LiPSs. When the LiPSs reaches the amorphous Al2O3 film, more stable chemical bonds are formed between Al3+ and S2-, Li+ and O2- to achieve more effective adsorption and anchoring of LiPSs. At 1C with a high sulfur loading up to 3-5 mg cm-2 the LSB contributes a specific charge capacity of 717.4 mAh g-1, with high retention rate up to 75.49 % after 450 cycles. The U-shaped electrolytic cell experiment and ultraviolet-visible spectrum experiment confirmed the LiPSs barrier function of the functional separator.

16.
Mol Ther ; 30(4): 1564-1577, 2022 04 06.
Article in English | MEDLINE | ID: mdl-35051616

ABSTRACT

Oral squamous cell carcinoma (OSCC), which is typically preceded by oral leukoplakia (OL), is a common malignancy with poor prognosis. However, the signaling molecules governing this progression remain to be defined. Based on microarray analysis of genes expressed in OL and OSCC samples, we discovered that the long non-coding RNA IFITM4P was highly expressed in OSCC, and ectopic expression or knockdown of IFITM4P resulted in increased or decreased cell proliferation in vitro and in xenografted tumors, respectively. Mechanistically, in the cytoplasm IFITM4P acted as a scaffold to facilitate recruiting SASH1 to bind and phosphorylate TAK1 (Thr187), and in turn to increase the phosphorylation of nuclear factor κB (Ser536) and concomitant induction of PD-L1 expression, resulting in activation of an immunosuppressive program that allows OL cells to escape anti-cancer immunity in cytoplasm. In nucleus, IFITM4P reduced Pten transcription by enhancing the binding of KDM5A to the Pten promoter, thereby upregulating PD-L1 in OL cells. Moreover, mice bearing tumors with high IFITM4P expression had notable therapeutic sensitivity to PD-1 monoclonal antibody (mAb) treatment. Collectively, these data demonstrate that IFITM4P may serve as a new therapeutic target in blockage of oral carcinogenesis, and PD-1 mAb can be an effective reagent to treat OSCC.


Subject(s)
Carcinoma, Squamous Cell , Mouth Neoplasms , RNA, Long Noncoding , Animals , Antibodies, Monoclonal , B7-H1 Antigen/metabolism , Carcinogenesis/genetics , Carcinoma, Squamous Cell/metabolism , Cell Line, Tumor , Mice , Mouth Neoplasms/genetics , Mouth Neoplasms/metabolism , Programmed Cell Death 1 Receptor , RNA, Long Noncoding/genetics
17.
Clin Oral Investig ; 26(1): 1089-1093, 2022 Jan.
Article in English | MEDLINE | ID: mdl-35043219

ABSTRACT

OBJECTIVES: To investigate the changes of T helper cell (Th)1/Th2-related cytokine expression in the saliva of minor recurrent aphthous stomatitis (RAS) patients before and after treatment with systemic prednisone. METHODS: A total of 101 patients with RAS and 15 participants with normal oral mucosa as controls were enrolled in this study. The levels of cytokine expression in the whole unstimulated saliva were examined using a multiplex bead-based cytometric bead array before and after prednisone treatment at a starting dose of 15 mg/day. RESULTS: The levels of salivary interleukin (IL)-4, IL-5, IL-6, IL-10, interferon (IFN)-γ, and tumor necrosis factor-α (TNF-α) in RAS patients were significantly higher than those of the normal controls (all P < 0.001). Importantly, the levels of salivary IL-6, IL-10, IFN-γ, and TNF-α in RAS patients were significantly decreased following prednisone treatment (all P < 0.001). Moreover, the IFN-γ to IL-4 ratio (mean: 26.9) was significantly (P < 0.001) decreased after treatment, which almost returned to normal (mean: 24.4; P > 0.05). CONCLUSION: This preliminary study demonstrates for the first time that prednisone exerts a significant therapeutic role against RAS through decreasing salivary cytokine levels and promoting a Th1/Th2 balance. CLINICAL RELEVANCE: Salivary cytokine profiles may provide a noninvasive, convenient, and effective approach to monitoring the course of RAS and may even be helpful to identify key pathogenic factors and potential mechanisms.


Subject(s)
Saliva , Stomatitis, Aphthous , Cytokines , Humans , Interferon-gamma , Prednisone/therapeutic use , Stomatitis, Aphthous/drug therapy , Th1 Cells , Th2 Cells
18.
J Hazard Mater ; 421: 126819, 2022 01 05.
Article in English | MEDLINE | ID: mdl-34396960

ABSTRACT

This study investigated the dosage-effect of biochar on the suppressed mesophilic digestion of oily sludge (OS) containing naphthalene (recalcitrant compound) and starch (easily bioavailable substrate). Methanogenesis was inhibited in control with OS, where biomethane yield (63.33 mL/gVS) was obviously lower than theoretical yield (260.55 mL/gVS). With adding optimal dose of biochar (0.60 g/gVS OS), the highest CH4 yield (138.41 mL/gVS) was 2.19 times of control. Meanwhile, the efficiencies of hydrolysis, acidogenesis and acetogenesis were significantly enhanced. However, excessive biochar (4.80 g/gVS OS) caused negative effects with methanogenic efficiency diminished by 32.5% and lag phase prolonged by 5.72 h. Dissolved organic matter (DOM) analysis showed that humic acid-like and fulvic acid-like components percentages of fluorescence regional integration were decreased because of the adsorption of biochar. In addition, biochar mediating interspecies electron transfer selectively enriched electroactive fermentation bacteria (Clostridium and Bacteroides) and acetoclastic Methanosaeta, which was responsible for promoting mesophilic digestion performance. The functional genes related to metabolism and environmental information processing were potentially activated by biochar. Above results indicate that moderate biochar application may mitigate the bio-toxicity suppression of OS, which help to provide a promising pathway for reinforcing oily wastes bio-treatment.


Subject(s)
Microbiota , Sewage , Anaerobiosis , Bioreactors , Charcoal , Methane
19.
Water Res ; 210: 117969, 2022 Feb 15.
Article in English | MEDLINE | ID: mdl-34952458

ABSTRACT

[Bmim]FeCl4, or 1­butyl­3-methylimidazolium tetrachloroferrate, is a typical ionic liquid (IL). Its recyclable, magnetic, multicomponent, and solvent-free nature makes it a particularly attractive ionic liquid for use in industrial processes. Despite its widespread use, the potential hazards that [Bmim]FeCl4 might pose to the environment, including productive microorganisms, have not been explored. In this study, the dose-response of [Bmim]FeCl4 in anaerobic digestion (AD) was investigated to assess the potential toxification and biochar-dependent detoxification in microbial communities, including enzymatic activity and molecule docking dynamics. Our results showed that methane production (31.52 mLmax/gVS) was sharply inhibited following [Bmim]FeCl4 treatment. Moreover, increasing the dosage of [Bmim]FeCl4 caused more dissolved organic matter (DOM) to be generated. Interestingly, 0.4 g/L of [Bmim]FeCl4 could stimulate the high activity of microbial hydrolase and ATPase. However, a higher concentration of 2.65 g/L prevented these enzymatic processes from continuing. At the cellular level, higher concentration of [Bmim]FeCl4 (>0.4 g/L) increased malondialdehyde (MDA) levels, leading to a higher cell lethal rate and weakening of the secondary structures of protein (especially, the amide I region). At the molecular level, the competitive H-bonding in the active sites caused low activity and consummated more energy. At the community level, structural equation modeling (SEM) revealed that [Bmim]FeCl4 and biochar were the main drivers for microbial community succession. For instance, high [Bmim]FeCl4 (8 g/L) benefited the growth of Clostridium sensu_stricto (from ≤1% to 27%). It is worth mentioning that biochar reversed the inhibition with high α-diversity, which caused a resurgence in the activity of previously inhibited ATPase and hydrolase. H2-trophic methanogens (Methanolinea and Methaofastidisoum) were sensitive to [Bmim]FeCl4 and decreased linearly while acetoclastic methanogens (Methanosaeta) were unchanged. These findings were consistent with the short-term activity tests and further verified by functional analysis.


Subject(s)
Charcoal , Dissolved Organic Matter , Anaerobiosis , Kinetics
20.
Biodivers Data J ; 10: e87316, 2022.
Article in English | MEDLINE | ID: mdl-36761582

ABSTRACT

Background: Twenty-eight Libnotes Westwood, 1876 species belonging to three subgenera have been known to occur in China, of which 13 belong to the nominotypical subgenus. Amongst the 13 Chinese Libnotes (s. str.) species, eight are from the Chinese mainland and five are from Taiwan. New information: A Libnotes (s. str.) species from Jilin, China, L. (L.) changbaishana sp. nov. is described and illustrated as new to science. The genus Libnotes is recorded from Jilin Province for the first time. The new species can be distinguished from congeners mainly by its body colour, wing and male genitalia.

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