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1.
Chin Med J (Engl) ; 126(6): 1138-43, 2013 Mar.
Article in English | MEDLINE | ID: mdl-23506594

ABSTRACT

BACKGROUND: Since an effective method for generating induced pluripotent stem cells (iPSCs) from human neural stem cells (hNSCs) can offer us a promising tool for studying brain diseases, here we reported direct reprogramming of adult hNSCs into iPSCs by retroviral transduction of four defined factors. METHODS: NSCs were successfully isolated and cultured from the hippocampus tissue of epilepsy patients. When combined with four factors (OCT3/4, SOX2, KLF4, and c-MYC), iPSCs colonies were successfully obtained. RESULTS: Morphological characterization and specific genetic expression confirmed that these hNSCs-derived iPSCs showed embryonic stem cells-like properties, which include the ability to differentiate into all three germ layers both in vitro and in vivo. CONCLUSION: Our method would be useful for generating human iPSCs from NSCs and provide an important tool for studying neurological diseases.


Subject(s)
Induced Pluripotent Stem Cells/cytology , Induced Pluripotent Stem Cells/metabolism , Neural Stem Cells/cytology , Neural Stem Cells/metabolism , Cell Differentiation/genetics , Cell Differentiation/physiology , Cells, Cultured , Cellular Reprogramming/genetics , Cellular Reprogramming/physiology , Humans , Immunohistochemistry , Kruppel-Like Factor 4 , Kruppel-Like Transcription Factors/metabolism , Octamer Transcription Factor-3/metabolism , Proto-Oncogene Proteins c-myc/metabolism , Reverse Transcriptase Polymerase Chain Reaction , SOXB1 Transcription Factors/metabolism
2.
Shanghai Kou Qiang Yi Xue ; 13(1): 2-5, 2004 Feb.
Article in Chinese | MEDLINE | ID: mdl-15007469

ABSTRACT

PURPOSE: We assessed the value of 16-layer three-dimensional computed tomography angiography (3D-CTA) and digital subtraction angiography (DSA) in diagnosis of the arteriovenous malformations (AVM). METHODS: 16 patients with AVM were examined by 16 layers CT scan and DSA. RESULTS: The DSA imaging provided excellent visualization in all of patients, including size, location, and feeding arterial of AVM. The value of the diagnosis of the nidus malformation vascular by 3D-CTA was similar to that by DSA, however, CTA was not good at diagnosis of the third feeding artery of AVM. The three-dimensional reconstruction also further revealed the relation of lesion and surrounding tissue which is helpful in making an appropriate decision before treatment. Differences in dates were compared using the t test. P<0.05 was considered statistically significant. CONCLUSION: 16-layer 3D-CTA was a superior approach for diagnosis and follow up of the AVM.


Subject(s)
Angiography, Digital Subtraction , Arteriovenous Malformations/diagnostic imaging , Imaging, Three-Dimensional , Tomography, X-Ray Computed , Adolescent , Adult , Arteriovenous Malformations/therapy , Child , Female , Humans , Male , Middle Aged
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