ABSTRACT
In West Africa intestinal parasites diseases and lack of food are quoted among the main causes of childhood morbidity. This study aimed to assess both the frequency and the relationships between anthropometric values and haemogramme troubles among children living in a West tropical forest area. A cross-sectional survey was tested on a sample of 262 children drawn at two levels. The parameters studied were the weight, height, age, sex, percentiles at height per age and weight per height ratios, haemogramme and intestinal parasites. The results revealed that microcyte hypochrome anemia is generally dominant in the haematological profile. The intestinal parasites carriage has a frequency higher than 68%, but has no influence on either the haematological profile or the anthropometric status. However, the haematological troubles found during that experience revealed a strong association with weight-height ratio troubles. Furthermore, malnutrition signs were observed.
Subject(s)
Intestinal Diseases, Parasitic , Adolescent , Anthropometry , Child , Cote d'Ivoire/epidemiology , Cross-Sectional Studies , Female , Humans , Intestinal Diseases, Parasitic/blood , Intestinal Diseases, Parasitic/epidemiology , MaleSubject(s)
Acute-Phase Proteins/analysis , Anemia, Sickle Cell/blood , Lipoprotein(a)/blood , Adolescent , Anemia, Sickle Cell/genetics , Child , Child, Preschool , Female , Homozygote , Humans , Infant , MaleABSTRACT
Two acute phase proteins; C-reactive protein and acid alpha 1-glycoprotein and three nutritional markers; prealbumin, retinol binding protein and transferrin have been evaluated in 8 patients suffering from trypanosomiasis in meningoencephalitic state and compared to those obtained from 15 normal control subjects. Findings show a markly decrease of nutritional markers without change of sera acute phase proteins. We concluded that in meningo-encephalitic state of human african trypanosomiasis, denutrition was a major biological or clinical feature in association with lymphoid cells stimulation as revealed by beta 2-M levels.
Subject(s)
Acute-Phase Proteins/analysis , Meningoencephalitis/parasitology , Nutritional Physiological Phenomena , Trypanosomiasis, African/blood , Adolescent , Adult , Aged , B-Lymphocytes/immunology , Biomarkers/blood , C-Reactive Protein/analysis , Female , Humans , Hypergammaglobulinemia/etiology , Hypergammaglobulinemia/immunology , Lymphocyte Activation , Male , Meningoencephalitis/blood , Middle Aged , Nutrition Disorders/etiology , Orosomucoid/analysis , Prealbumin/analysis , Retinol-Binding Proteins/analysis , T-Lymphocytes/immunology , Transferrin/analysis , Trypanosomiasis, African/complications , Trypanosomiasis, African/immunologyABSTRACT
L'Etude a porte sur le dosage serique de la beta2-microglobiline (B2-m) et de la ferritine (Ft) chez 60 patients souffrant du syndrome d'immuno deficience acquise (SIDA) repartis en fonction des affections associees. Les resultats obtenus montrent que la B2-m est precocement augmentee dans l'infection a VIH alors que l'elevation de la Ft n'apparait que dans le SIDA maladie. Ceci suggere que ces deux parametres biologiques devraient faire partie du bilan biologique de l'infection a VIH et constitueraient ainsi un mini-profil proteique de la surveillance des sujets asymptomatiques seropositifs aux anticorps anti-VIH
ABSTRACT
Plasma apolipoprotein AI, apolipoprotein B, cholesterol and triglycerides in 114 Ivorian patients with homozygous sickle cell anaemia (HbSS) were compared to 40, age and sex matched controls with normal haemoglobin (HbAA). Except for plasma triglycerides, lipid and apolipoprotein levels were found to be lower in patients with sickle cell anaemia as compared to normal control subjects. The differences between the two groups were found to be statistically significant (p < 0.05 Student's t test). In addition, a highly significant decrease of plasma apolipoprotein AI (p < 0.01) was observed during painful crisis as compared to the steady state (0.78 g.L-1 versus 1.01 g.L-1), whereas triglyceride levels were found to be higher (1.02 g.L-1 versus 0.78 g.L-1). Although the metabolism of lipoproteins is being altered, the results of this cross-sectional study do not suggest that patients with sickle cell anaemia are at higher risk of developing coronary artery disease.
Subject(s)
Anemia, Sickle Cell/blood , Apolipoproteins A/blood , Apolipoproteins B/blood , Lipids/blood , Adolescent , Adult , Child , Child, Preschool , Cholesterol/blood , Cholesterol, HDL/blood , Cholesterol, LDL/blood , Cote d'Ivoire , Female , Humans , Male , Triglycerides/bloodABSTRACT
The main clinical use of measurements of Apo Al and Apo B is to determine a patient's risk status for the development of ischemic heart disease. Apo B is generally accepted as a contributory cause of coronary artery disease, while Apo Al apparently has a protective effect. The present study reports the plasma change of Apo Al and B in sickle cell anaemic subjects. Immunochemical findings revealed that Apo Al and B levels were found to be lower in sickle cell patients as compared with normal subjects (HbAA). The atherogenicity index given by Apo B/Apo Al remained in the normal range during periods of steady state (0.64 +/- 0.28), while subjects in painful crisis had high index values (0.95 +/- 0.35). Therefore, we concluded that epidemiological studies on a large sample of patients are needed to confirm this relationship between painful crisis and risk of developing coronary artery disease.
Subject(s)
Anemia, Sickle Cell/blood , Anemia, Sickle Cell/complications , Apolipoprotein A-I/blood , Apolipoproteins B/blood , Arteriosclerosis/etiology , Lipids/blood , Adolescent , Adult , Case-Control Studies , Child , Child, Preschool , Cote d'Ivoire , Female , Humans , Risk FactorsABSTRACT
Prospectively assessment of nutritional status, besides anthropometric parameters measurements, included determination of the following plasma proteins: albumin, transferrin, retinol-binding protein and thyroxine-binding prealbumin, usually regarded as useful indices of protein depletion states. Patients' inflammatory or infectious diseases were investigated by measuring the two most reliable acute-phase reactants (orosomucoid and c-reactive protein). This study was conducted on a group of 56 pediatric patients malnourished and 54 age- and sex-matched healthy subjects as controls. Data showed a higher prevalence of inflammatory process during the course of malnutrition as revealed progressive but severe alteration of the Prognostic Inflammatory and Nutritional Index (PINI) or CRP x a1-GPA/Alb x TBPA. On the other hand, kwashiorkor and marasmus seemed to be a precipitating cause of infectious or/and inflammatory process. We, therefore, conclude that the PINI scoring system should be for the pediatricians, a sensitive and precious tool allowing the correct follow-up of inflammatory pole in nutritional disorders in developing countries.
Subject(s)
Blood Proteins/metabolism , Nutrition Disorders/blood , C-Reactive Protein/metabolism , Child, Preschool , Cote d'Ivoire , Female , Humans , Infant , Inflammation/blood , Inflammation/complications , Male , Nutrition Disorders/complications , Orosomucoid/metabolism , Prospective Studies , Retinol-Binding Proteins/metabolism , Retinol-Binding Proteins, Plasma , Serum Albumin/metabolism , Thyroxine-Binding Proteins/metabolism , Transferrin/metabolismABSTRACT
L'extrait de feuilles seches de Persea americana; traditionnellement utilise pour ses proprietes antihypertensives a ete teste par le suivi biologique et clinique de 5 malades qui ont volontairement choisi cette forme de therapeutique. L'efficacite therapeutique de l'extrait a ete jugee remarquable. Cependant; quelques effets secondaires ont ete releves (une asthenie liee a l'hypokaliemie induite et la chute des cheveux) qui ont contraint a l'arret du traitement chez les 3 patients ages de moins de 45 ans. Ces resultats; tout en confirmant l'activite pharmacologique de certains extraits; doivent inciter a une recherche plus approfondie afin d'eviter de possibles desagrements en rapport avec une toxicite chronique
Subject(s)
Antihypertensive Agents , Hypertension/drug therapy , Medicine , PlantsABSTRACT
Two acute phase reactants (C-reactive protein: CRP and alpha 1-acid glycoprotein: alpha 1-AGP) and transferrin (Tf) levels change were measured in order to evaluate their clinical or prognostic value during the vaso-occlusive crisis. Measurements were performed in normal controls, painful crisis with or without treatment and during steady state. Immunochemical determinations indicate that markedly acute inflammation occurred in sickle cell painful crisis, as revealed CRP and alpha 1-AGP levels. Compared to controls, during periods of steady state in the absence of any intercurrent condition, a lesser but significative increase of alpha 1-GPA was observed. Transferrin level remained lower and did not sufficient to discriminate between crisis and steady state. Compared to the vasodilatator drug effect, non steroidal anti-inflammatory drug showed more efficiency in the sickle cell disease inflammatory process. Taken together, these findings indicate that serial determinations of CRP, alpha 1-GPA and Tf may be helpful in monitoring the course of sickle cell disease and response to treatment. Then, non steroidal anti-inflammatory drugs appear necessary to reduce the length of the sickle cell crisis.
Subject(s)
Anemia, Sickle Cell/blood , Anemia, Sickle Cell/immunology , C-Reactive Protein/analysis , Orosomucoid/analysis , Transferrin/analysis , Acute Disease , Adolescent , Adult , Anemia, Sickle Cell/drug therapy , Anti-Inflammatory Agents, Non-Steroidal/therapeutic use , Case-Control Studies , Child , Child, Preschool , Female , Humans , Inflammation , Male , PrognosisABSTRACT
Le profil de variation de deux proteines de la reaction inflammatoire : la proteine C-reactive (CRP) et l'a1-glycoproteine acide a1-GPA ou orosomucoide et de la transferrine(TF) a ete etudie; afin d'evaluer leur interet clinique et/ou pronostique; au cours de la crise vasculo-occlusive de la drepanocytose homozygote; traitee et non traitee. Cet ensemble de parametres biochimiques devrait alors faire partie de tout bilan de la drepanocytose et constituerait ainsi un mini-profil proteique de surveillance et du traitement de la maladie drepanocytaire
Subject(s)
Anemia , Anemia/complications , Anemia/diagnosis , Anemia/drug therapy , Anemia/prevention & control , Anti-Inflammatory Agents , C-Reactive Protein , Glycoproteins , Orosomucoid , TransferrinABSTRACT
A protein profile has been monitored during human immunodeficiency virus (HIV) infection. The investigation concerned 60 patients suffering from acquired immunodeficiency syndrome (AIDS), 24 asymptomatic HIV-antibody seropositive subjects and 22 healthy HIV-antibody seronegative, individuals voluntary blood donors. Data show that retinol-binding protein, thyroxin-binding prealbumin and beta 2-microglobulin are already modified in HIV infection (p less than 0.05) whereas the other protein alteration becomes apparent during AIDS. These studies demonstrate that severe, but progressive malnutrition occurs in patients with AIDS. On the other hand nutritional abnormalities can be shown to have a deleterious effect upon the disease course as revealed by increasing alpha-1-acid glycoprotein and C-reactive protein levels for 60 to 70% of patients.
Subject(s)
Acquired Immunodeficiency Syndrome/blood , HIV Seropositivity/blood , Inflammation/blood , Nutrition Disorders/blood , Adult , Biomarkers/blood , Blood Proteins/analysis , Female , Humans , MaleABSTRACT
C-reactive protein (CRP) and alpha 1-acid glycoprotein (alpha 1-GPA) two acute phase reactants, have both been monitored in patients suffering from hepatocellular diseases, compared with healthy subjects. Immunochemical findings, in hepatic amebiasis, revealed a higher incidence of increased alpha 1-GPA levels (86% of patients, as compared to 46% for CRP), whereas during liver primitive cancer and cirrhosis inverse pattern occurs. In viral chronic hepatitis, lesser perturbations were observed. In contrast, a simultaneous increase of both proteins is strong supporting evidence for the severity of disease with an unfavourable prognostic.
Subject(s)
C-Reactive Protein/analysis , Hepatitis/metabolism , Orosomucoid/analysis , Adolescent , Adult , Aged , Child , Child, Preschool , Female , Hepatitis, Chronic/metabolism , Humans , Liver Abscess, Amebic/metabolism , Liver Cirrhosis/metabolism , Liver Neoplasms/metabolism , Male , Middle AgedABSTRACT
Using ultrafiltration and SDS-PAGE, abnormal urinary protein excretion was found in 25.4% of 189 persons with sickle cell disease and trait, but none of 72 controls. Based upon molecular weight of urinary proteins, underlying renal lesions were classified as glomerular, tubular, or both. Altered protein excretion appeared at an early age, was more abnormal in older subjects, and was related to the severity of sickle cell disease (SS greater than SC = S/beta Thal greater than AS). Since none of the subjects had yet developed clinically significant renal disease, SDS-PAGE may permit early detection of patients who require careful follow-up or aggressive therapy.
Subject(s)
Anemia, Sickle Cell/complications , Proteinuria/etiology , Adolescent , Adult , Age Factors , Anemia, Sickle Cell/urine , Child , Electrophoresis, Polyacrylamide Gel , Female , Humans , Male , Molecular Weight , Proteinuria/classification , Proteinuria/urine , Sex Factors , UltrafiltrationSubject(s)
Hydrocortisone/blood , Adult , Cote d'Ivoire , Female , Fluorometry , Humans , Male , Reference ValuesABSTRACT
A new extraction procedure of urinary estrogens, based upon adsorption chromatography on Amberlite XAD-2, was deviced for a selective and complete extraction of these components. Estrogens were then quantified, without preliminar hydrolysis, by the colorimetric Kober reaction, modified by Ittrich. The reliability of this method has been established by comparison with four other methods.
