Subject(s)
Enteritis/etiology , Intestinal Diseases/complications , Intestine, Small/blood supply , Ischemia/etiology , Aged , Constriction, Pathologic/complications , Constriction, Pathologic/surgery , Enteritis/diagnosis , Enteritis/surgery , Humans , Intestinal Diseases/surgery , Intestine, Small/surgery , Ischemia/diagnosis , Ischemia/surgery , Male , Treatment OutcomeABSTRACT
Polysaccharides extracted from human tubercle bacilli (specific substance of Maruyama) have been clinically applied in patients with malignant diseases in Japan and other countries. It is known that increased colorectal carcinogenesis occurs in patients with ulcerative colitis. The repeated mucosal necrosis-regeneration sequence in chronic ulcerative colitis induced with 3 % dextran sulfate sodium led to colorectal carcinogenesis in azoxymethane-pretreated mice. Simultaneously multiple injections with the polysaccharides reduced the increases in thymidylate synthase and thymidine kinase activities and a number of bromodeoxyuridine-incorporated S-phase cells in colorectal tissues resulted in the reduction of tumorous regions with high-grade dysplasia.
Subject(s)
Colorectal Neoplasms/prevention & control , Polysaccharides, Bacterial/therapeutic use , Animals , Azoxymethane , Bacillus/chemistry , Body Weight/drug effects , Bromodeoxyuridine/metabolism , Carcinogens , Colitis, Ulcerative/chemically induced , Colitis, Ulcerative/enzymology , Colon/drug effects , Colon/enzymology , Colon/pathology , Colorectal Neoplasms/chemically induced , Colorectal Neoplasms/enzymology , Dextran Sulfate , Female , Mice , Mice, Inbred CBA , Rectum/drug effects , Rectum/enzymology , Rectum/pathology , S Phase/drug effects , Specific Pathogen-Free Organisms , Thymidine Kinase/metabolism , Thymidylate Synthase/metabolismABSTRACT
A new antiulcer agent, ecabet sodium is one of dehydroabietic acid derivatives prepared from pine resin. The effects of ecabet sodium on colorectal carcinogenesis were investigated in azoxymethane-pretreated mice with chronic ulcerative colitis induced by 3 repeated administration of 3% dextran sulfate sodium and in 1, 2-dimethylhydrazine-treated rats. Although daily treatment with ecabet sodium did not affect the colorectal DNA-synthesizing enzyme activities and bromodeoxyuridine-immunoreactive S-phase cells, high-grade dysplasia in ecabet sodium-treated mice was less frequent than in untreated mice. In rats, ecabet sodium administration reduced the elevated activity of thymidylate synthetase in colorectal tumors.
