ABSTRACT
The novel quinoline alkaloid scolopendrine was isolated from the centipede, Scolopendra subspinipes mutilans L. Koch. The structure was determined to be 2-hydroxy-7-[(4-hydroxy-3-methoxyphenyl)methyl]-3-methoxy-8-quinolyl sulfate on the basis of high-resolution electron-spray ionization mass spectroscopy and two-dimensional NMR spectral data. Unlike quinoline alkaloids so far reported, scolopendrine is unique in having a 7-benzyl moiety in the quinoline ring.
Subject(s)
Alkaloids/chemistry , Anisoles/chemistry , Arachnida/chemistry , Indoles/chemistry , Alkaloids/isolation & purification , Animals , Anisoles/isolation & purification , Chromatography, Thin Layer , Indoles/isolation & purification , Magnetic Resonance Spectroscopy , Spectrometry, Mass, Electrospray Ionization , Spectrometry, Mass, Fast Atom BombardmentABSTRACT
We report herein the cases of two patients who underwent complete resection of a superior sulcus tumor (SST) plus adjuvant brachytherapy, with the area to be irradiated determined by a computer program system designed to minimize unnecessary irradiation to the normal components and to optimize the effect on the targeted area. Although the efficacy of brachytherapy on the inhibition of local relapse needs to be observed over a long period, the selective and alternative use of delivering adjuvant brachytherapy by this method appears to enhance the quality of life of patients with a SST.
Subject(s)
Brachytherapy/methods , Lung Neoplasms/radiotherapy , Aged , Humans , Lung Neoplasms/pathology , Lung Neoplasms/surgery , Male , Neoplasm Recurrence, Local/prevention & control , Radiotherapy, Adjuvant , Treatment OutcomeABSTRACT
The extracellular phospholipase B gene from baker's yeast Torulaspora delbrueckii was cloned and sequenced. Analysis of DNA sequence data revealed an open reading frame (ORF) encoding a 649-amino acid protein, that included amino acid sequences obtained from the purified enzyme. Comparison of these sequence data with the N-terminal amino acid sequence of the enzyme indicated that this secreted protein is synthesized as a large precursor with a 21-amino acid N-terminal extension to the mature enzyme of 628 amino acids. A homology search was carried out between phospholipase B from T. delbrueckii and Penicillium notatum. The deduced amino acid sequence of the cloned phospholipase B was homologous (about 50% identity) to phospholipase B from P. notatum, and contained six conserved regions. The transcriptional level of mRNA of the phospholipase B gene was higher in the cells from early exponential and stationary phases.
