Heavy metal exposure and behavioral assessment of vultures in a captive environment.

Heavy metals are a heterogeneous group of metals that can cause detrimental biological disruptions in vultures. Heavy metals play a threatening role and affect vulture populations around the globe. However, various conservation organizations are working for the captive breeding of these endangered animals so that they can reintroduce these captive-bred animals back to their natural environment. This research study was conducted at Changa Manga Vulture Conservatory, Punjab, with the help of Punjab Wildlife Department. A non-invasive technique was used to collect vulture feathers and fecal samples to study the exposure of heavy metals. The behavior of vultures in captivity was also recorded. Samples were chemically digested in aqua regia and analyzed for the detection of five heavy metal concentrations such as chromium (Cr), cadmium (Cd), lead (Pb), zinc (Zn), and magnesium (Mg) using flame atomic absorption spectroscopy (FAAS). Among the selected heavy metals, non-essential metals such as Pb, Cr, and Cd were recorded in the least concentration, whereas essential metals Zn and Mg were recorded in the highest concentration. Behavioral studies showed that vultures in captivity become excessively hostile and afraid of any human interaction; they flew to the top of the trees if they felt any danger. It was concluded in the study that the higher levels of both essential and non-essential metals recorded in feathers and fecal samples might be due to the type of feed, even though a controlled food is provided to vultures in captivity. However, regular monitoring of the environment can bring improvements to the health of vultures in captivity and help to find accurate sources of contamination.

Assessment of Respiratory Problems in Workers Associated with Intensive Poultry Facilities in Pakistan.

BACKGROUND: The poultry industry in Pakistan has flourished since the 1960s; however, there are scarce data regarding the impact of occupational exposure on the pulmonary health of farm workers in terms of years working in the industry. The objective of the present study was to assess the effect of poultry environment on the health of occupationally exposed poultry farmers in countries of warm climatic regions, such as Pakistan. This study will also show the effect of exposure to poultry facilities on the health of poultry farmers in the context of low-income countries with a relatively inadequate occupational exposure risk management. MATERIALS AND METHODS: The lung function capacity of 79 poultry workers was measured using a spirometer. Along with spirometry, a structured questionnaire was also administrated to obtain information about age, height, weight, smokers/nonsmokers, years of working experience, and pulmonary health of farm workers. The workers who were directly involved in the care and handling of birds in these intensive facilities were considered and divided into four groups based on their years of working experience: Group I (3-10 months), Group II (1-5 years), Group III (6-10 years), and Group IV (more than 11 years). The forced vital capacity (FVC), forced expiratory volume in one second (FEV1) and the FEV1/FVC ratio were considered to identify lung function abnormalities. Statistical analysis was carried out using independent sample t test, Chi-square test, Pearson's correlation, and linear regression. RESULTS: Based on the performed spirometry, 68 (86 %) of workers were found normal and healthy, whereas 11 (14 %) had a mild obstruction. Of the 11 workers with mild obstruction, the highest number with respect to the total was in Group IV (more than 11 years of working experience) followed by Group III and Group II. Most of the workers were found healthy, which seems to be because of the healthy survivor effect. For the independent sample t test, a significant difference was noticed between healthy and nonhealthy farmers, whereas Chi-square test showed a significant association with height, drugs, and working experience. Linear regression that was stratified by respiratory symptoms showed for workers with symptoms, regression models for all spirometric parameters (FVC, FEV1, and FEV1/FVC) have better predictive power or R square value than those of workers without symptoms. CONCLUSION: These findings suggest that lung function capacity was directly related to years of working experience. With increasing number of working years, symptoms of various respiratory problems enhanced in the poultry workers. It should be noted that most of the poultry workers were healthy and young, the rationale being that there is a high turnover rate in this profession. The mobility in this job and our finding of 86% of the healthy workers in the present study also proposed healthy worker survivor effect.

Estimation of particulate matter and gaseous concentrations using low-cost sensors from broiler houses.

Particulate and gaseous emissions from intensive poultry facilities are major public and environmental health concern. The present study was aimed at exploratively monitoring particulate matter (PM) and gaseous concentrations in controlled-environment facilities using low-cost sensors in Lahore, Pakistan. The indoors and outdoors of 18 broiler houses, grouped into three categories based on the age of birds: group I (1-20 days), group II (21-30 days) and group III (31-40 days), were examined. Low-cost sensors Dylos 1700 and Aeroqual 500 series with different gas sensor heads were used to monitor PM and different gases such as nitrogen dioxide (NO2), hydrogen sulphide (H2S), carbon dioxide (CO2) and methane (CH4), respectively. Overall, the mean PM and gaseous concentrations increased with the age and activity of birds as compared with the non-activity time of birds. Statistically significant differences were observed in all measured parameters among the groups. The negative correlation between indoor and outdoor environments for PM and gas concentrations at some broiler houses demonstrates the contribution of additional sources to emissions in outdoor environments. The findings contribute to our knowledge of temporal characteristics of particulate and gaseous concentrations from poultry facilities particularly in Pakistan and generally to the capability of using low-cost sensors to evaluate emissions from such facilities.

Assessment of heavy metals in different organs of cattle egrets (Bubulcus ibis) from a rural and urban environment in Pakistan.

The study was conducted to evaluate the concentration of essential elements (Cu, Fe, Mn, Ni, Se, Zn, and B) and non-essential elements (Cd, Pb, Hg, Cr, As, and Ni) in muscle, liver, bone, and intestine of matured cattle egret (Bubulcus ibis). Sampling was carried out at two sites of Lahore, Pakistan-Havalian Karbath (site I) and Mehmood Booti (site II)-over a period of 1 month in the winter season. Metal analyses of samples were carried out using inductively coupled plasma mass spectroscopy (ICPMS). The trend of essential elements in liver and intestine of site I was noticed as Fe > Zn > Cu > B > Mn > Se > Ni and almost same for bone and muscle as Fe > Zn > B > Mn > Cu > Se > Ni. It was noticed that Cu was less deposited in bone and muscle tissues compared to liver and intestine from site I. The deposition of essential elements in liver and intestine from site II was noticed as Fe > Zn > Cu > Mn > B > Se > Ni. Similar trend was found for bone and muscle: Fe > Zn > B > Mn > Cu > Se > Ni with great deposition of B than Cu compared to liver and intestine. The findings of the present study revealed almost similar trend for essential elements deposition at both sites. However, a random trend was observed for deposition of non-essential elements (Ag, As, Ba, Cd, Cr, Hg, Pb) in organs from both sites. Moreover, data showed higher levels of non-essential elements accumulation (particularly As, Ba, and Pb) in the body tissues/organs of cattle egret in an urban area (site II) as compared to rural area (site I) of Lahore. Furthermore, non-essential elements were more in intestine, bone, and muscles from site II showing more exposure to some non-essential elements at urban site due to human and natural activities. However, higher concentration of non-essential elements in liver from site I as compared to other organs not only reflect the land exposure to plant growth promoting fertilizers and sewage water for irrigation purpose but also better detoxification abilities of the rural birds. The study gave a new insight to inform contamination levels in the rural and urban sites. Future implications of this study need remediation strategies to clean environment requisite for avian species.