Assessment of nitrogen metabolism in rats on the background of acute toxic hepatitis and it's correction with l-arginin and l-ornitin.

The liver is the main organ responsible for the metabolism of different substances. At the same time it is the primary target organ for many toxic chemicals, which are metabolized there. Carbon tetrachloride is a wellknown hepatotoxin widely used to induce acute toxic liver injury in a wide range of laboratory animals. This substance induces oxidative damage, inflammation and fibrosis in the liver. AIM: The aim is to evaluate the peculiarities of nitrogen metabolism in rats on the background of acute toxic hepatitis and its correction with L-arginin and L-ornitin. MATERIALS AND METHODS: The study was performed on 40 outbred white male rats with experimental hepatitis, caused by carbon tetrachloride. The animals were divided into five groups: control group (the rats were simulated carbon tetrachloride poisoning and its correction by administering of olive oil and normal saline in equivalent doses), acute carbon tetrachloride hepatitis (single intraperitoneal injection of 50% carbon tetrachloride oil solution at the dose of 2 ml/kg-1 of body weight and simulation of treatment by administration of normal saline in equivalent doses), acute carbon tetrachloride hepatitis + L-ornithine (1000 mg×kg-1), acute carbon tetrachloride hepatitis + L-arginine (500 mg×kg-1) and acute carbon tetrachloride hepatitis + combination of substances. RESULTS: On the background of acute carbon tetrachloride intoxication, it was observed the development of toxic hepatitis in experimental animals, manifested by significant increasing of urea and creatinine levels in the blood serum of animals with a simultaneous decreasing of nitrite anion level. The administration of L-ornithine and L-arginine demonstrates positive impact on liver status and functions by stabilization of cell membranes and regeneration of functional capacity of injured cells. CONCLUSIONS: The results of our study confirm both the presence of unidirectional effects and absence of toxic influences of L-ornithine and L-arginine on liver cells under the conditions of acute carbon tetrachloride intoxication, which are the most important requirements for modern drugs for the treatment of hepato-renal syndrome.

HEALTH DYNAMICS OF THE MEDICAL UNIVERSITY STUDENTS DURING SPORTS ACTIVITIES.

OBJECTIVE: The aim: Is to investigate the dynamics of the morphofunctional development and physical health of students who were engaged in strength sports while studying at university. PATIENTS AND METHODS: Materials and methods: The study involved 360 male students of different faculties between the ages of 17 and 20. Two groups of students were formed: experimental and control groups. The EG students (n=40) were engaged in strength sports (powerlifting, athletics, Crossfit); the CG students (n=320) were training according to the current program of physical education. The study of the morphofunctional development of students was carried out taking account of the indicators of body length, body weight, handgrip test, heart rate, blood pressure, and vital capacity. The level of students' health was examined according to the methodology of the assessment of the physical health level by G. L. Apanasenko. RESULTS: Results: It was established that strength sports at university affect physical development, functional abilities of the major systems of an organism, and the health state of future doctors in a more efficient way than the current program of physical education. It was found that the influence on the indicators of handgrip test, vital capacity, and heart rate of the EG students was the most prominent positive effect of sports. The evaluation of the calculated indexes (power index, life index) and the level of physical health confirmed this trend. CONCLUSION: Conclusions: The conducted research asserts the necessity of introducing the sports-oriented form of the physical training organization at the medical higher education institutions of Ukraine to strengthen the students' health and to maintain the efficiency of the future doctors' professional activity.

Improvement of students' morpho-functional development and health in the process of sport-oriented physical education.

OBJECTIVE: The aim is to investigate the influence of sambo wrestling classes on the level of morpho-functional development and health of students during their studying. PATIENTS AND METHODS: Materials and methods: The investigation was conducted in Zhytomyr National Agroecological University in 2017-2019. Ninety four students (51 male and 43 female) took part in the research. Two experimental and two control groups were formed: EG1 (n=25) and CG1 (n=26) involved male students, EG2 (n=21) and CG2 (n=22) - female students. The classes of EG were held according to the authors' methodology, the classes of CG - according to the current physical education program. The analysis of the indicators of weight, height, lungs capacity, handgrip test, heart rate, blood pressure, body mass index, life index, power index and Robinson's index was performed. The interrelation of students of EG and CG was defined according to the health levels at the beginning and at the end of the investigation. RESULTS: Results: It is determined that sambo wrestling classes had a positive influence on the students' muscles system improvement, increased lung capacity, reduced body mass, improved cardiovascular system, and improved health. CONCLUSION: Сonclusions: The improvement of the indicators of morpho-functional development and health of students during sambo wrestling classes will have a positive influence on their physical working capacity and the efficiency of their studying and future professional activities.

THE EFFECTS OF L-ORNITINE AND L-ARGININE ON THE PROCESSES OF LIPID PEROXIDATION IN THE FUNCTIONAL LAYERS OF KIDNEYS ON THE BACKGROUND OF ACUTE TOXIC HEPATITIS.

OBJECTIVE: The aim is to evaluate the effects of L-arginine and L-ornithine on the processes of lipid peroxidation in homogenates of renal cortex, renal medulla and renal papilla under conditions of acute toxic hepatitis. PATIENTS AND METHODS: Materials and methods: The study was performed on 40 outbred white male rats with experimental hepatitis, caused by carbon tetrachloride. The animals were divided into five groups: control group (the rats were simulated carbon tetrachloride poisoning and its correction by administering of olive oil and normal saline in equivalent doses), acute carbon tetrachloride hepatitis (single intraperitoneal injection of 50% carbon tetrachloride oil solution at the dose of 2 mlxkg-1 of body weight and simulation of treatment by administration of normal saline in equivalent doses), acute carbon tetrachloride hepatitis + L-ornithine (1000 mgxkg-1), acute carbon tetrachloride hepatitis + L-arginine (500 mgxkg-1) and acute carbon tetrachloride hepatitis + combination of substances. RESULTS: Results: On the background of acute carbon tetrachloride intoxication it was observed the development of renal failure in experimental animals, manifested by activation of lipid peroxidation processes in homogenates of renal cortex, renal medulla and renal papilla. The administration of L-ornithine and L-arginine demonstrates positive impact on renal function and hepato-renal syndrome by stabilization of cell membranes and regeneration of functional capacity of injured renal cells. CONCLUSION: Conclusions: The results of our study confirm both the presence of unidirectional effects and absence of toxic influences of L-ornithine and L-arginine on renal cells under the conditions of acute carbon tetrachloride intoxication, which are the most important requirements for modern drugs for the treatment of hepato-renal syndrome.