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1.
Microscopy (Oxf) ; 73(1): 31-36, 2024 Feb 07.
Article in English | MEDLINE | ID: mdl-37300556

ABSTRACT

This article describes a neural network system for predicting the secondary electron yield of metallic materials. For bulk metals, experimental values are used as training data. Due to the strong correlation between the secondary electron yield and the work function, deep learning predicts the secondary electron yield with relatively high accuracy even with a small amount of training data. Our approach demonstrates the importance of the work function in predicting the secondary electron yield. For the secondary electron yield of thin metal films on metal substrates, deep learning predictions are generated using training data obtained by Monte Carlo simulations. The accuracy of the secondary yield predictions of thin films on substrates could be improved by adding experimental values of bulk metals to the training data.

2.
Mod Rheumatol ; 33(4): 851-855, 2023 Jul 04.
Article in English | MEDLINE | ID: mdl-35788855

ABSTRACT

INTRODUCTION: Total knee arthroplasty (TKA) for a stiff knee of patients with haemophilia (PWH) represents a challenge for orthopaedic surgeons for the difficulties of exposing the knee and high complication rate compared to a flexible knee. AIM: To optimize the surgical exposure in primary TKA for PWH and to propose a threshold angle of extension contracture in treating haemophilic knee joints, retrospectively. METHODS: Sixty-seven primary TKAs for PWH (mean age, 48 years) were performed, and incisional approaches to joint were standard (58 cases) and V-Y quadricepsplasty (V-Y) (9 cases). The decision of surgical approach was decided intraoperatively by two surgeons. Pre- and post-knee angles were evaluated in each group. Variables in the V-Y group were evaluated using univariate logistic regression analysis and receiver operating characteristic curve analysis. RESULTS: Univariate logistic regression analysis demonstrated that the preoperative range of motion (ROM) and flexion were significantly associated with V-Y. Threshold values of preoperative flexion and ROM resulting in V-Y using receiver operating characteristic analysis were 45° and 35°, respectively. CONCLUSIONS: Primary TKA for PWH using a standard approach may be performed before the stage preoperative flexion <45° and ROM <35°.


Subject(s)
Arthroplasty, Replacement, Knee , Contracture , Hemophilia A , Joint Diseases , Humans , Middle Aged , Arthroplasty, Replacement, Knee/adverse effects , Arthroplasty, Replacement, Knee/methods , Hemophilia A/complications , Hemophilia A/surgery , Retrospective Studies , Treatment Outcome , Knee Joint/surgery , Contracture/etiology , Contracture/surgery , Range of Motion, Articular
3.
Nanomaterials (Basel) ; 12(15)2022 Jul 27.
Article in English | MEDLINE | ID: mdl-35957005

ABSTRACT

A hybrid smart process and material design system for nanoimprinting is proposed, which is combined with a learning system based on experimental and numerical simulation results. Instead of carrying out extensive learning experiments for various conditions, the simulation learning results are partially complimented when the results can theoretically be predicted by numerical simulation. In other words, the data that are lacking in experimental learning are complimented by simulation-based learning results. Therefore, the prediction of nanoimprint results without experimental learning could be realized under various conditions, even for unknown materials. In this study, material and process designs are demonstrated for a low-temperature nanoimprint process using glycerol-containing polyvinyl alcohol. The experimental results under limited conditions were learned to investigate the optimum glycerol concentrations and process temperatures. Simulation-based learning was used to predict the dependence on press pressure and shape parameters. The prediction results for unknown glycerol concentrations agreed well with the follow-up experiments.

4.
Mod Rheumatol Case Rep ; 5(2): 414-420, 2021 07.
Article in English | MEDLINE | ID: mdl-33441049

ABSTRACT

Haemophilia is an X-linked congenital bleeding disorder caused by a deficiency of factor VIII/IX. Patients with haemophilia (PWH) experience spontaneous bleeding into joints and muscles. Recurrent joint bleeds result in painful and disabling haemophilic multi-arthropathy characteristic of elbows, knees and ankles. The standard of care for PWH is replacement of factor concentrate. Haemophilic pseudotumor (HPT) is one of the complications which can occur in PWH due to repeated bleeding. The occurrence of HPT is not uniform, so treatment needs to be tailored to the individual. We report the case of right distal femur HPT with multi haemophilic end-staged arthropathies (bilateral elbows, knees and ankles). He suffered from walking disability and right thigh pain. He showed functional limitations in those arthropathies, so he could not use crutches. To reduce excess loads on affected joints, we performed left total knee arthroplasty before excision of HPT of the right femur. This is the first case report of a 37-year-old man with haemophilia whose treatment combined en bloc excision of the HPT and reconstruction of distal femur using a tumour prosthesis with severe bone destruction after excision of HPT. At the 24-month postoperative follow-up, the patient was able to walk without any support. When the patients suffer from multi-joint haemophilic arthropathy and HPT, comprehensive and well-planned surgical treatment strategy under adequate factor VIII replacement therapy is necessary.


Subject(s)
Bone Diseases , Hematoma , Hemophilia A , Adult , Bone Diseases/surgery , Hematoma/etiology , Hematoma/surgery , Hemophilia A/complications , Humans , Male , Severity of Illness Index
5.
Food Chem ; 252: 265-270, 2018 Jun 30.
Article in English | MEDLINE | ID: mdl-29478540

ABSTRACT

Tofuyo, a Japanese traditional food, is a fermented soybean curd manufactured in Okinawa region. Due to its original cheese-like flavor, the current study was designed to evaluate the sensory and chemical characteristics of three stepwise ultrafiltration fractions, using 10,000, 3000 and 500 Da membranes and further chromatographic fractions from tofuyo. The results showed that umami, sweet and salty were the characteristic tastes of all fractions, with umami intensity evaluated for the fraction with MW less than 500 Da (F-500) as the most prominent among the three fractions. Subsequent Sephadex G-25 SF fractions and RP-HPLC fractions were subjected to sensory and chemical analyses. The tastiest fraction contained sodium chloride, sugars, organic acids, umami and sweet free amino acids, at concentrations above their thresholds. The abundant presence of umami and sweet free amino acids with certain concentrations of sodium chloride and glucose might provide the typical savory taste of tofuyo.


Subject(s)
Fermented Foods/analysis , Soy Foods/analysis , Amino Acids/analysis , Chromatography, High Pressure Liquid , Flavoring Agents/analysis , Humans , Japan , Molecular Weight , Sodium Chloride/analysis , Taste
6.
Sci Rep ; 7(1): 2825, 2017 06 06.
Article in English | MEDLINE | ID: mdl-28588196

ABSTRACT

Optical tweezers based on optical radiation pressure are widely used to manipulate nanoscale to microscale particles. This study demonstrates direct measurement of the optical force gradient distribution acting on a polystyrene (PS) microsphere using a carbon nanotube (CNT) mechanical resonator, where a PS microsphere with 3 µm diameter is welded at the CNT tip using laser heating. With the CNT mechanical resonator with PS microsphere, we measured the distribution of optical force gradient with resolution near the thermal noise limit of 0.02 pN/µm in vacuum, in which condition enables us to high accuracy measurement using the CNT mechanical resonator because of reduced mechanical damping from surrounding fluid. The obtained force gradient and the force gradient distribution agree well with theoretical values calculated using Lorenz-Mie theory.

7.
Front Neurosci ; 11: 57, 2017.
Article in English | MEDLINE | ID: mdl-28239336

ABSTRACT

Evidence is increasing that brain areas that are responsible for action planning and execution are activated during the information processing of action-related verbs (e.g., pick or kick). To obtain further evidence, we conducted three experiments to see if constraining arm posture, which could disturb the motor planning and imagery for that arm, would lead to delayed judgment of verbs referring to arm actions. In all experiments, native Japanese speakers judged as quickly as possible whether the presented object and the verb would be compatible (e.g., ball-throw) or not (e.g., ball-pour). Constrained arm posture was introduced to the task by asking participants to keep both hands behind their back. Two types of verbs were used: manual action verbs (i.e., verbs referring to actions performed on an object by a human hand) and non-manual action verbs. In contrast to our hypothesis that constrained arm posture would affect only the information processing of manual action verbs, the results showed delayed processing of both manual action and non-manual action verbs when the arm posture was constrained. The effect of constrained arm posture was observed even when participants responded with their voice, suggesting that the delayed judgment was not simply due to the difficulty of responding with the hand (i.e., basic motor interference). We discussed why, contrary to our hypothesis, constrained arm posture resulted in delayed CRTs regardless of the "manipulability" as symbolized by the verbs.

8.
Sci Rep ; 6: 22600, 2016 Mar 03.
Article in English | MEDLINE | ID: mdl-26935657

ABSTRACT

Despite the superb intrinsic properties of carbon nanotube mechanical resonators, the quality factors at room temperature are 1,000 or less, even in vacuum, which is much lower than that of mechanical resonators fabricated using a top-down approach. This study demonstrates the improvement of the quality factor and the control of nonlinearity of the mechanical resonance of the cantilevered nanotube by electrostatic interaction. The apparent quality factor of the nanotube supported by insulator is improved drastically from approximately 630 to 3200 at room temperature. Results show that retardation of the electrostatic force induced by the contact resistance between the nanotube and the insulator support improves the quality factor. Finite element method calculation reveals that the nonuniform pileup charge on the insulator support strongly influences the nonlinearity of the resonance.

9.
J Food Sci Technol ; 52(3): 1839-48, 2015 Mar.
Article in English | MEDLINE | ID: mdl-25745266

ABSTRACT

In this work, chemical and biological characteristics of two types of Thai fermented shrimp paste, Kapi Ta Dam and Kapi Ta Deang, at different fermentation periods and their raw materials were investigated. Kapi had low water activity and high proteins with high glutamic acid and lysine. Both Kapis, which had different sources, showed similar characteristics. The number of lactic acid bacteria in the products increased during the early stages of fermentation. Free α-amino acid contents in the products increased with the fermentation time. The water extracts from Kapi products showed strong antioxidative activities against ABTS(+) radical, and ACE inhibitory activity but they did not exhibit antimicrobial activity against Staphylococcus aureus, Bacillus cereus, Escherichia coli and Salmonella Typhimurium. Biological activities in Kapi could be developed by fermentation process, enzymatic hydrolysis of proteins and non-enzymatic browning reactions. Kapi could, thus, serve as a potential source of natural bioactive substances.

10.
Exp Brain Res ; 232(3): 753-64, 2014 Mar.
Article in English | MEDLINE | ID: mdl-24306437

ABSTRACT

Perception of the fit between a person's action capabilities and relevant environmental properties (i.e., affordances) is often fine tuned gradually through experience performing a behavior. However, the immediate effect of such practice on the improvement of affordance perception is unclear. The present study was designed to examine whether a critical factor in the immediate effect of such practice is the opportunity to detect very fine differences between possible and impossible behaviors [i.e., high-resolution (HR) practice]. Participants reported whether apertures of various widths were passable when walking while holding a 69-cm horizontal bar (Experiment 1) or when using a wheelchair (Experiment 2). When practicing passing through apertures, seven different aperture widths, including their minimum passable width (70 cm for both experiments) were presented around the affordance boundary with 1- or 5-cm increments for the HR or low-resolution (LR) conditions, respectively. Accuracy of perception of passability improved following both HR and LR practice when walking. In contrast, no improvement was observed in any condition when using a wheelchair. These findings suggest that the immediate effect of practice was mediated by the form of locomotion but not the resolution of the practice.


Subject(s)
Practice, Psychological , Psychomotor Performance/physiology , Space Perception/physiology , Walking/physiology , Wheelchairs , Adolescent , Adult , Analysis of Variance , Biomechanical Phenomena , Female , Humans , Male , Upper Extremity/physiology , Young Adult
11.
Clin Oral Implants Res ; 25(8): 977-82, 2014 Aug.
Article in English | MEDLINE | ID: mdl-23745964

ABSTRACT

OBJECTIVE: Periodontal pathogens in dental plaque are the main causative agents of periodontitis and peri-implantitis. Detection of the presence of such periodontal pathogens early would serve as a useful tool in the diagnosis and treatment of this disease. Therefore, the purpose of this study was to investigate whether the periodontal pathogen levels in saliva were correlated with the periodontal status of patients receiving implant treatment. MATERIALS AND METHODS: A total of 291 patients visiting Tokyo Dental College Chiba Hospital were divided into four groups: a no-periodontitis (np) group, a mild-periodontitis (mip) group, a moderate-periodontitis (mop) group, and a severe-periodontitis (sp) group. The levels of the following five periodontal pathogens in saliva were evaluated using real-time polymerase chain reaction: Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Treponema denticola, and Prevotella intermedia. RESULTS: The levels of P. gingivalis and T. forsythia were significantly higher in mop group than in np group (P < 0.05). The levels of all periodontal pathogens tested except A. actinomycetemcomitans were significantly higher in sp group than in np group (P < 0.05). CONCLUSION: The detection levels of the periodontal pathogens targeted in saliva samples were correlated with the periodontal status. This suggests that using saliva to screen for periodontopathic bacteria offers an easier-to-use clinical tool than the paper point method in the diagnosis and treatment of periodontitis and peri-implantitis.


Subject(s)
Periodontitis/microbiology , Saliva/microbiology , Adult , Colony Count, Microbial , Dental Implantation , Female , Humans , Japan , Male , Middle Aged , Preoperative Care , Real-Time Polymerase Chain Reaction , Severity of Illness Index
12.
Appl Microbiol Biotechnol ; 96(1): 49-60, 2012 Oct.
Article in English | MEDLINE | ID: mdl-22864970

ABSTRACT

Red koji or red mold rice is prepared by growing a genus Monascus on steamed rice. For centuries, it has been used in Asia for the production of fermented foods including red rice wine and fermented tofu. Although red koji is an important source of various hydrolytic enzymes critical for food fermentation, information on the enzymatic properties in red koji has been limited. Hydrolytic enzymes produced by Monascus fungi may play important roles in ripening of tofuyo (Japanese fermented tofu) regarding the chemical and physical properties of the product. This review provides an introduction of red koji, its properties, and the application of hydrolytic enzymes, especially aspartic proteinases and carboxypeptidases from Monascus fungi. We also describe tofuyo and a novel fermented soybean protein food using a microbial action originating from red koji.


Subject(s)
Food Microbiology , Monascus/enzymology , Monascus/metabolism , Oryza/metabolism , Asia , Oryza/microbiology , Soy Foods/microbiology
13.
Bull Tokyo Dent Coll ; 52(1): 53-7, 2011.
Article in English | MEDLINE | ID: mdl-21467782

ABSTRACT

Periodontal disease is considered a risk factor in dental implant treatment. The purpose of this study was to investigate the periodontal conditions in patients requesting dental implant therapy. A total of 169 patients visiting Department of Oral and Maxillo-Facial Implantology at Tokyo Dental College Chiba Hospital were targeted. The following intraoral parameters were measured in each patient: Community Periodontal Index (CPI) score, probing pocket depth (PPD), clinical attachment level (CAL) and bleeding on probing (BOP). Prevalence of patients with periodontal pockets was high: 38% and 28% of patients had a CPI score of code 3 and 4, respectively. Prevalence of teeth with one or more sites with PPD≥4mm was 27%. Moreover, clinical signs suggestive of periodontitis (PPD, CAL≥4mm) were found in 10-15% of tooth sites. Prevalence rates at sites with severe periodontal breakdown (PPD, CAL≥7mm) were 2-5%. These results further emphasize the importance of thorough periodontal assessment in patients prior to dental implant treatment.


Subject(s)
Dental Implantation, Endosseous , Periodontal Diseases/diagnosis , Contraindications , Humans , Middle Aged , Periodontal Index , Risk Factors
14.
J Ind Microbiol Biotechnol ; 38(9): 1485-92, 2011 Sep.
Article in English | MEDLINE | ID: mdl-21298320

ABSTRACT

An acid proteinase from Monascus purpureus No. 3403, MpuAP, was previously purified and some characterized in our laboratory (Agric Biol Chem 48:1637-1639, 1984). However, further information about this enzyme is lacking. In this study, we investigated MpuAP's comprehensive substrate specificity, storage stability, and prospects for reducing antigenicity of whey proteins for application in the food industry. MpuAP hydrolyzed primarily five peptide bonds, Gln(4)-His(5), His(10)-Leu(11), Ala(14)-Leu(15), Gly(23)-Phe(24) and Phe(24)-Phe(25) in the oxidized insulin B-chain. The lyophilized form of the enzyme was well preserved at 30-40°C for 7 days without stabilizers. To investigate the possibility of reducing the antigenicity of the milk whey protein, enzymatic hydrolysates of the whey protein were evaluated by inhibition ELISA. Out of the three main components of whey protein, casein and α-lactalbumin were efficiently degraded by MpuAP. The sequential reaction of MpuAP and trypsin against the whey protein successfully degraded casein, α-lactalbumin and ß-lactoglobulin with the highest degree of hydrolysis. As a result, the hydrolysates obtained by using the MpuAP-trypsin combination showed the lowest antigenicity compared with the single application of pepsin, trypsin or pepsin-trypsin combination. Therefore, the overall result suggested that the storage-stable MpuAP and trypsin combination will be a productive approach for making hypoallergic bovine milk whey protein hydrolysates.


Subject(s)
Milk Proteins/immunology , Milk Proteins/metabolism , Monascus/enzymology , Peptide Hydrolases/metabolism , Animals , Antigens/immunology , Antigens/metabolism , Caseins/metabolism , Cattle , Hydrolysis , Lactalbumin/metabolism , Lactoglobulins/metabolism , Papain/metabolism , Pepsin A/metabolism , Protein Hydrolysates/immunology , Trypsin/metabolism , Whey Proteins
15.
J Food Sci ; 75(3): R71-6, 2010 Apr.
Article in English | MEDLINE | ID: mdl-20492309

ABSTRACT

Soy sauce taste has become a focus of umami taste research. Umami taste is a 5th basic taste, which is associated to a palatable and pleasurable taste of food. Soy sauce has been used as an umami seasoning since the ancient time in Asia. The complex fermentation process occurred to soy beans, as the raw material in the soy sauce production, gives a distinct delicious taste. The recent investigation on Japanese and Indonesian soy sauces revealed that this taste is primarily due to umami components which have molecular weights lower than 500 Da. Free amino acids are the low molecular compounds that have an important role to the taste, in the presence of sodium salt. The intense umami taste found in the soy sauces may also be a result from the interaction between umami components and other tastants. Small peptides are also present, but have very low, almost undetected umami taste intensities investigated in their fractions.


Subject(s)
Soy Foods , Taste , Amino Acids/analysis , Flavoring Agents/chemistry , Food Handling/methods , Humans , Sodium Chloride, Dietary/analysis , Soy Foods/analysis
16.
Appl Microbiol Biotechnol ; 86(6): 1867-75, 2010 May.
Article in English | MEDLINE | ID: mdl-20107983

ABSTRACT

A gram-positive thermotolerant bacterium, designated strain RKK-04, was isolated from a fermented Thai fish sauce broth as it demonstrated high proteolytic activity. A phylogenetic analysis based on comparisons of 16S rRNA gene sequences showed that strain RKK-04 is Bacillus licheniformis. The proteolytic enzyme, which was purified 80-fold with 18% yield, has a molecular mass of 31 kDa and an isoelectric point higher than 9.3. The optimum pH and temperature of the enzyme activity were found to be 10.0 and 50 degrees C, respectively. The addition of diisopropyl fluorophosphate and phenylmethanesulfonyl fluoride completely inhibited enzymatic activity. These results showed that the enzyme is a subtilisin-like alkaline serine proteinase. On the other hand, the enzyme exhibited unique cleavage sites in oxidized insulin B-chain that differed from those of other subtilisin-like proteases. High enzymatic activity was also retained under high salt conditions (30% NaCl). The myosin heavy chain of fish protein was completely digested by reaction with this enzyme. Thus the halotolerant proteinase from B. licheniformis RKK-04 is a key enzyme for fish sauce fermentation.


Subject(s)
Bacillus/enzymology , Condiments/microbiology , Fish Products/microbiology , Serine Proteases/isolation & purification , Serine Proteases/metabolism , Actomyosin/metabolism , Amino Acid Sequence , Bacillus/isolation & purification , Bacterial Proteins/chemistry , Bacterial Proteins/isolation & purification , Bacterial Proteins/metabolism , Fermentation , Fish Proteins/metabolism , Food Microbiology , Hydrogen-Ion Concentration , Insulin/metabolism , Metals/pharmacology , Molecular Sequence Data , Molecular Weight , Phenylmethylsulfonyl Fluoride/pharmacology , Serine Proteases/chemistry , Sodium Chloride/pharmacology , Substrate Specificity , Subtilisin/chemistry , Subtilisin/metabolism , Temperature
17.
FEMS Microbiol Lett ; 288(2): 266-72, 2008 Nov.
Article in English | MEDLINE | ID: mdl-19054086

ABSTRACT

The oxidizing enzyme NAD(+)-dependent polypropylene glycol dehydrogenase (PPG-DH) was purified to homogeneity from the cytoplasmic fraction of Stenotrophomonas maltophilia grown on polypropylene glycol (diol type) 2000. The purified enzyme consisted of a homotetrameric protein (37 kDa subunit) with a molecular mass of around 154 kDa. The N-terminal amino acid sequence (25 residues) showed similarity to the sequences of NAD(+)-dependent secondary alcohol dehydrogenases and NADH-dependent reductases. The enzyme preferentially oxidized medium-chain secondary alcohols, di- and tri-propylene glycols and polypropylene glycols including those with secondary alcohol groups in their molecular structure. Consequently, the enzyme was classified into a group of NAD(+)-dependent secondary alcohol dehydrogenases.


Subject(s)
Alcohol Dehydrogenase , NAD/metabolism , Polymers/metabolism , Propylene Glycols/metabolism , Stenotrophomonas maltophilia/enzymology , Alcohol Dehydrogenase/chemistry , Alcohol Dehydrogenase/isolation & purification , Alcohol Dehydrogenase/metabolism , Amino Acid Sequence , Cytoplasm/enzymology , Molecular Sequence Data , Stenotrophomonas maltophilia/genetics , Stenotrophomonas maltophilia/growth & development , Stenotrophomonas maltophilia/isolation & purification , Substrate Specificity
18.
Biosci Biotechnol Biochem ; 71(10): 2573-6, 2007 Oct.
Article in English | MEDLINE | ID: mdl-17928688

ABSTRACT

Two forms of an extracellular glucoamylase, MpuGA-I and MpuGA-II, were purified to homogeneity from Monascus purpureus RY3410. The molecular weights of these enzymes were estimated to be 60,000 (MpuGA-I) and 89,000 (MpuGA-II). These enzymes were glycoproteins with a carbohydrate content of 15.0% (MpuGA-I) and 16.2% (MpuGA-II) respectively. The pH optima were 5.0 for both enzymes, and the optimal temperatures were 50 degrees C (MpuGA-I) and 65 degrees C (MpuGA-II). The Km values for soluble starch were calculated to be 4.0+/-0.8 mg/ml (MpuGA-I) and 1.1+/-0.2 mg/ml (MpuGA-II) respectively.


Subject(s)
Glucan 1,4-alpha-Glucosidase/isolation & purification , Glucan 1,4-alpha-Glucosidase/metabolism , Monascus/enzymology , Amino Acid Sequence , Carbohydrates/analysis , Chromatography, Gel , Electrophoresis, Polyacrylamide Gel , Enzyme Activation/drug effects , Enzyme Stability , Glucan 1,4-alpha-Glucosidase/analysis , Glucan 1,4-alpha-Glucosidase/antagonists & inhibitors , Glucan 1,4-alpha-Glucosidase/chemistry , Hydrogen-Ion Concentration , Hydrolysis , Isoelectric Point , Kinetics , Metals, Heavy/pharmacology , Molecular Sequence Data , Molecular Weight , Phylogeny , Sequence Homology, Amino Acid , Solubility , Starch/metabolism , Substrate Specificity , Temperature
19.
Microbiology (Reading) ; 152(Pt 7): 1941-1949, 2006 Jul.
Article in English | MEDLINE | ID: mdl-16804170

ABSTRACT

A gene for periplasmic poly(vinyl alcohol) (PVA) dehydrogenase (PVADH) was cloned, based on the N-terminal amino acid sequence of the purified PVADH from Sphingomonas sp. 113P3 and the sequence of the gene for PVADH (pvaA, GenBank accession no. AB190288). The recombinant PVADH tagged with hexahistidine was expressed in Escherichia coli and purified to homogeneity. The recombinant enzyme had the same characteristics as the purified enzyme from Sphingomonas sp. strain 113P. In addition to PVA, the recombinant PVADH could oxidize glycols such as polypropylene glycols and 1,3-butane/cyclohexanediol and 2,4-pentanediol, but neither primary nor secondary alcohols. The amino acid sequence of the recombinant PVADH showed similarity with those of PVADH from Pseudomonas sp. strain VM15C, putative PVADHs from Azoarcus sp. EbN1, and Xanthomonas species (54-25 % identity), and the quinohaemoprotein alcohol dehydrogenases (QH-ADHs) from Comamonas testosteroni, Ralstonia eutropha and Pseudomonas putida (25-29 % identity). PVADHs from strains 113P3 and VM15C have a conserved superbarrel domain (SD), probable PQQ-binding amino acids in the SD and a haem-binding domain (HBD) (they should be designated QH-PVADHs), but the positions of the amino acid sequences for the HBD and SD are the reverse of those of QH-ADHs. A protein structure of QH-PVADHs is proposed. Results of dot-blot hybridization and RT-PCR indicated that the three genes encoding oxidized PVA hydrolase, PVADH and cytochrome c are expressed constitutively and form an operon.


Subject(s)
Alcohol Oxidoreductases/genetics , Periplasm/enzymology , Sphingomonas/enzymology , Alcohol Oxidoreductases/chemistry , Amino Acid Sequence , Cloning, Molecular , Molecular Sequence Data , Nucleic Acid Hybridization , Reverse Transcriptase Polymerase Chain Reaction , Sphingomonas/genetics
20.
J Ind Microbiol Biotechnol ; 32(10): 487-9, 2005 Oct.
Article in English | MEDLINE | ID: mdl-16179984

ABSTRACT

The actions of pepsin and the admixture of pepsin and Monascus pilosus carboxypeptidase 1 (MpiCP-1) on the hydrolysis of soybean protein were studied. The results showed that the pepsin hydrolyzate of soybean protein was much more bitter and contained relatively smaller amounts of total free amino acids than the hydrolyzate obtained with the admixture of pepsin and MpiCP-1. In addition, hydrophilic and hydrophobic amino acids were present in almost equal proportions in the pepsin hydrolyzate, while mainly hydrophobic amino acids made up the hydrolyzate obtained with the admixture of pepsin and MpiCP-1. These results suggest that MpiCP-1 suppresses and reverses the development of the bitterness taste that results from the pepsin hydrolysis of soybean protein by releasing mainly hydrophobic amino acids from the C-termini of the bitter components.


Subject(s)
Carboxypeptidases/metabolism , Monascus/enzymology , Soybean Proteins/metabolism , Hydrolysis/drug effects , Pepsin A/pharmacology
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