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PLoS One ; 17(2): e0263822, 2022.
Article in English | MEDLINE | ID: mdl-35157705

ABSTRACT

Diffuse intrinsic pontine glioma (DIPG) is a lethal pediatric brain tumor. While there are a number of in vivo rodent models for evaluating tumor biology and response to therapy, these models require significant time and resources. Here, we established the chick-embryo chorioallantoic (CAM) assay as an affordable and time efficient xenograft model for testing a variety of treatment approaches for DIPG. We found that patient-derived DIPG tumors develop in the CAM and maintain the same genetic and epigenetic characteristics of native DIPG tumors. We monitored tumor response to pharmaco- and radiation therapy by 3-D ultrasound volumetric and vasculature analysis. In this study, we established and validated the CAM model as a potential intermediate xenograft model for DIPG and its use for testing novel treatment approaches that include pharmacotherapy or radiation.


Subject(s)
Antineoplastic Agents/pharmacology , Brain Stem Neoplasms/genetics , Chorioallantoic Membrane/drug effects , Chorioallantoic Membrane/radiation effects , Diffuse Intrinsic Pontine Glioma/genetics , Angiogenesis Inhibitors/pharmacology , Angiogenesis Inhibitors/therapeutic use , Animals , Antineoplastic Agents/therapeutic use , Brain Stem Neoplasms/drug therapy , Brain Stem Neoplasms/pathology , Brain Stem Neoplasms/radiotherapy , Cell Line, Tumor , Chick Embryo , Chorioallantoic Membrane/pathology , Diffuse Intrinsic Pontine Glioma/drug therapy , Diffuse Intrinsic Pontine Glioma/pathology , Diffuse Intrinsic Pontine Glioma/radiotherapy , Humans , Rats , Ultrasonography , Xenograft Model Antitumor Assays
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