ABSTRACT
Chronic low-grade inflammation has been suggested as a key contributor of the pathogenesis and development of polycystic ovary syndrome (PCOS). To investigate the association between oxidative stress status and inflammatory cytokines in follicular fluid of 21 PCOS women compared to 21 women with normal ovarian function who underwent intra-cytoplasmic sperm injection. Concentration of IL-6, IL-8, IL-10, and TNF-α was measured using sandwich ELISA. Oxidative stress was examined by measuring total oxidant status (TOS), malondialdehyde (MDA), total antioxidant capacity (TAC), and thiol groups. PCOS women had an elevated concentration of MDA and TOS compared to controls. Levels of TAC and thiol groups were lower in PCOS compared to controls. PCOS patients had a higher concentration of IL-6, IL-8, and TNF-α compared to controls. Concentration of IL-10 was lower in PCOS compared to controls. Significant correlations were found between MDA and TOS concentration with TNF-α and between IL-6 and MDA, IL-8 and TAC, IL-10 and TOS levels and also between IL-10 and TAC levels. TAC and thiol groups were negatively correlated with TNF-α. Increased oxidative stress in PCOS is associated with inflammation which is closely linked. Inflammation can induce production of inflammatory cytokines in this syndrome and directly stimulates excess ovarian androgen production.
Subject(s)
Interleukin-6/metabolism , Interleukin-8/metabolism , Ovary/immunology , Oxidative Stress , Polycystic Ovary Syndrome/immunology , Tumor Necrosis Factor-alpha/metabolism , Up-Regulation , Adult , Biomarkers/metabolism , Cross-Sectional Studies , Down-Regulation , Female , Follicular Fluid/immunology , Follicular Fluid/metabolism , Hospitals, University , Humans , Infertility, Female/etiology , Infertility, Female/therapy , Interleukin-10/metabolism , Iran , Middle Aged , Ovary/metabolism , Ovary/physiopathology , Polycystic Ovary Syndrome/metabolism , Polycystic Ovary Syndrome/physiopathology , Sperm Injections, IntracytoplasmicABSTRACT
PURPOSE: To investigate the expression of Adaptor protein containing a PH domain, PTB domain and leucine zipper motif 1 (APPL1), insulin receptor (INSR), adiponectin and adiponectin receptors (adipoR1 and R2) and their possible associations in granulosa cells (GCs) of 22 polycystic ovary syndrome (PCOS) women compared to the 22 non-PCOS controls with normal ovulatory function matched for BMI (body mass index). METHODS: In this study, 44 infertile women aged 18-40 years undergoing in vitro fertilization (IVF) protocol were recruited. After follicular fluid collection, GCs were isolated and then purified with MACS (Micro Beads conjugated to monoclonal anti-human CD45 antibodies). RNA was extracted from GCs and quantitative real-time PCR (qRT-PCR) was performed to assess APPL1 gene expression. RESULTS: Expression of APPL1, insulin receptor and adiponectin system genes was significantly decreased in PCOS group compared to the controls. CONCLUSIONS: Reduction of APPL1, insulin receptor and adiponectin system genes in GCs could be involved in the development of PCOS.
Subject(s)
Adaptor Proteins, Signal Transducing/genetics , Adiponectin/genetics , Polycystic Ovary Syndrome/genetics , Receptor, Insulin/genetics , Receptors, Adiponectin/genetics , Adaptor Proteins, Signal Transducing/metabolism , Adiponectin/metabolism , Adolescent , Adult , Biomarkers/metabolism , Case-Control Studies , Cells, Cultured , Female , Granulosa Cells/cytology , Granulosa Cells/metabolism , Humans , Polycystic Ovary Syndrome/metabolism , Polycystic Ovary Syndrome/pathology , Real-Time Polymerase Chain Reaction , Receptor, Insulin/metabolism , Receptors, Adiponectin/metabolism , Young AdultABSTRACT
Despite advances in immunosuppressive therapy in the past decade, allograft rejection remains the primary cause for kidney graft failure. Cytokines are known to be important mediators in renal allograft outcome. The aim of the present study was to ascertain whether interleukin (IL)-4, IL-10, and transforming growth factor (TGF)-beta cytokine gene polymorphisms contributed to kidney graft outcome. We evaluated single nucleotide polymorphism in IL-4 (-1098G/T, -590C/T, -33C/T), IL-10 (-1082A/G, -819C/T, -592A/C), and TGF-beta (codon 10 and 25) in 100 renal transplant recipients and 139 normal healthy control using polymerase chain reactions based on sequence-specific primers. Recipients were clinically characterized as rejection episode (RE) versus stable graft function (SGF). The results showed the frequencies of IL-4 -33 T allele in the RE, SGF, and control group to be 7%, 73%, and 28%, respectively. IL-10 -592 A allele frequency was 39% in RE, 26% in SGF, and 28% in the control group. TGF-beta codon 10 T allele was 39% in RE, 35% in SGF, and 53% in control group. In conclusion, this study suggested that some cytokine gene alleles reflected SGF among kidney transplant recipients.