Antimicrobial resistance and genetic relatedness of Salmonella serotypes isolated from food, asymptomatic carriers, and clinical cases in Shiyan, China.

Salmonella is a primary cause of foodborne diseases globally. Despite food contamination and clinical infections garnering substantial attention and research, asymptomatic Salmonella carriers, potential sources of infection, have been comparatively overlooked. In this study, we conducted a comparative analysis of serotype distribution, antimicrobial resistance phenotypes, and genetic profiles of archived Salmonella strains isolated from food (26), asymptomatic carriers (41), and clinical cases (47) in Shiyan City, China. Among the 114 Salmonella strains identified, representing 31 serotypes and 34 Sequence Types (STs), the most prevalent serovars included Typhimurium, Derby, Enteritidis, Thompson, and London, with the most predominant STs being ST11, ST40, ST26, ST34, and ST155. Antimicrobial resistance testing revealed that all strains were only sensitive to meropenem, with 74.6% showing antimicrobial resistance (AMR) and 53.5% demonstrating multidrug resistance (MDR). Strains resistant to five and six classes of antibiotics were the most common. Pearson's chi-square test showed no statistically significant difference in the occurrence of AMR (p = 0.105) or MDR (p = 0.326) among Salmonella isolates from the three sources. Our findings underscore associations and diversities among Salmonella strains isolated from food, asymptomatic carriers, and clinical patients, emphasizing the need for increased vigilance towards asymptomatic Salmonella carriers by authorities.

Dissipation of Chlorpyrifos in Bottled Tea Beverages and the Effects of (-)-Epigallocatechin-3-Gallate.

ABSTRACT: Bottled tea beverages (BTB) are popular for the health benefits and convenience. Because chlorpyrifos (CP) is commonly used as a biomarker for exposure, as well as a pesticide in the field, it is important to determine the dynamics of CP dissipation in BTB to better perform risk assessments. This study focused on the dynamic behavior of CP for 22 days by fortifying bottled green tea, dark tea, and oolong tea beverages with the parent chemical and analyzing the degradation products. Photoinduction was used to generate the two transient intermediates: the reactive oxygen species from H2O2 and the triplet excited state of CP from the parent chemical in water were designed to observe the effects of (-)-epigallocatechin-3-gallate (EGCG) on the dissipation and transformation of CP. The results indicated that the CP degraded in BTB and the main products were detected. The half-life values of CP illustrated that EGCG increased the dissipation of CP by combination with CP and inhibited the generation of CP-oxon by scavenging the emerged oxidant, the reactive oxygen species, and interfering with the transformation of the triplet excited state of CP. This work suggests EGCG could play various roles in the dissipation and transformation of CP. Thus, a comprehensive identification of CP degradation should be performed when assessing the exposure risk in drinking BTB.

Arthroscopically assisted osteosynthesis of complex intra-articular fractures of the distal radius.

BACKGROUND: Complex intra-articular fractures of the distal radius pose a great challenge in orthopedic surgery. Conventional open reduction and internal fixation generally yields poor functional outcome. The technique of arthroscope-assisted surgery allows more accurate reduction of the articular surface and treatment of soft tissue lesions, both of which are necessary for regaining anatomic structure and satisfactory function. METHODS: Twenty wrists in 18 patients with intra-articular fracture of the distal radius received arthroscopic surgery and percutaneous pinning with or without external skeletal fixation and bone grafting. A new traction method was designed that allows arthroscopic surgery and fluoroscopic monitoring to be performed simultaneously. Postoperative follow-up averaged 24.7 months. RESULTS: All except one of these patients returned to work within 3 to 6 months after surgery. Later collapse with minimal articular step-off was noted on radiographs before osseous union in two patients who did not undergo bone grafting. Decreased radial height because of subsequent displacement at the metaphyseal fracture was noted in one patient who did not receive external skeletal fixation initially. CONCLUSION: The technique of arthroscope-assisted surgery, despite a steep learning curve, is an invaluable method. Results using this technique are promising and few complications are encountered in the treatment of complex radius platform fractures. The bone grafting procedure augments osseous union, shortens the duration of external skeletal fixation, and thus lessens joint stiffness.