ABSTRACT
Anoectochilus roxburghii (Wall.) Lindl is a perennial herb of the Orchidaceae family; a yellow-green mutant and a yellow mutant were obtained from the wild type, thereby providing good material for the study of leaf color variation. Pigment content analysis revealed that chlorophyll, carotenoids, and anthocyanin were lower in the yellow-green and yellow mutants than in the wild type. Transcriptome analysis of the yellow mutant and wild type revealed that 78,712 unigenes were obtained, and 599 differentially expressed genes (120 upregulated and 479 downregulated) were identified. Using the Kyoto Encyclopedia of Genes and Genomes pathway analysis, candidate genes involved in the anthocyanin biosynthetic pathway (five unigenes) and the chlorophyll metabolic pathway (two unigenes) were identified. Meanwhile, the low expression of the chlorophyll and anthocyanin biosynthetic genes resulted in the absence of chlorophylls and anthocyanins in the yellow mutant. This study provides a basis for similar research in other closely related species.
ABSTRACT
Polianthes tuberosa is a popular ornamental plant. Its floral scent volatiles mainly consist of terpenes and benzenoids that emit a charming fragrance. However, our understanding of the molecular mechanism responsible for the floral scent of P. tuberosa is limited. Using transcriptome sequencing and de novo assembly, a total of 228,706,703 high-quality reads were obtained, which resulted in the identification of 96,906 unigenes (SRA Accession Number SRP126470, TSA Acc. No. GGEA00000000). Approximately 41.85% of the unigenes were functionally annotated using public databases. A total of 4,694 differentially expressed genes (DEGs)were discovered during flowering. Gas chromatography-mass spectrometry analysis revealed that the majority of the volatiles comprised benzenoids and small amounts of terpenes. Homology analysis identified 13 and 17 candidate genes associated with terpene and benzenoid biosynthesis, respectively. Among these, PtTPS1, PtDAHPSs, PtPAL1, and PtBCMT2 might play important roles in regulating the formation of floral volatiles. The data generated by transcriptome sequencing provide a critical resource for exploring concrete characteristics as well as for supporting functional genomics studies. The results of the present study also lay the foundation for the elucidation of the molecular mechanism underlying the regulation of floral scents in monocots.
