ABSTRACT
Castanopsis carlesii (Hemsl.) Hay. is a widely distributed and dominant tree species with significant ecological and economical values. In this study, the complete chloroplast genome sequence of C. carlesii was reported using the Illumina Hiseq 2500 platform. The complete chloroplast genome was 160,205 bp forming a typical quadripartite structure, with a pair of inverted repeated (IRs) regions of 25,670 bp, a large single copy (LSC) region of 89,849 bp, and a small single copy (SSC) region of 19,016 bp. A total of 124 functional genes were annotated, including 79 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The ML phylogenetic analysis showed that the genus Castanopsis formed a clade except Castanopsis fargesii.
ABSTRACT
The Human Serum Albumin (HSA) can emit fluorescence (lamda(em) = 350 nm) under irradiation of ultraviolet light (lamda(ex) = 296 nm). After the vitamin B6 (B6) was added into HSA solution the fluorescence of HSA was quenched partially. This quenching was static quenching owing to the fact that ro (fluorescence life time (life time of the molecule excitation state) of HSA without B6) equaled to tau(i) (fluorescence life time of HSA with B6). The formation constant K between HSA and B6 was observed from the experiment, and then the binding distance R0 was determined (R0 = 1.872 nm). The CD spectra of samples (HSA, [HSA] = 1 x 10(-5) mol x L(-1), HSA +B6, [B6] = 5 x 10(-8), 1.5 x 10(-7), 2.5 x 10(-7), 3.5x 10(-7), 4.5x 10(-7) mol x L(-1)) were measured, and all CD spectra were almost the same. From [theta] values, the contents (percent content) of four structure(alpha-Helix, beta-Pleated, Beta-corner, and Randon wind) of samples can be calculated, and we find that all samples contain almost the same structure contents.
Subject(s)
Fluorescence , Serum Albumin/chemistry , Vitamin B 6/chemistry , Algorithms , Binding Sites , Circular Dichroism , Humans , Kinetics , Models, Chemical , Protein Binding , Serum Albumin/metabolism , Spectrometry, Fluorescence , Vitamin B 6/metabolismABSTRACT
Fluorescence spectroscopy has achieved great successes in the study on the interaction of drugs and DNA in both quantitative analysis and qualitative analysis. While the fluorescence probe technique can be used to measure the intensity of drugs-DNA interaction, because the injected drugs can change the fluorescence intensity of the probe. In this paper, the authors use berberine as a probe to measure the interaction intensity between some anticancer drugs and DNA, calculate the constant D and determine the effects of those drugs. In addition, the authors point out that this method can not be efficient for certain drug solution.
Subject(s)
Antineoplastic Agents/chemistry , Berberine/chemistry , DNA/chemistry , Spectrometry, Fluorescence/methods , Fluorouracil/chemistry , Kinetics , Mitomycins/chemistry , Reproducibility of ResultsABSTRACT
Spectral characteristics of an organic material in acid with different concentrations were investigated in the solution of a proper acid concentration. The results showed that at 252 nm the transmittance T = 56.5%, but at 290 nm the material had a strong absorption peak (epsilon > 10(4) L. (mol.cm)-1). The absorbance A280-298 nm > 8, i.e. T280-298 nm < 10(-8). The band width was about 16 nm. Therefore, this material has conspicuous characteristics such as high transmittance and deep cutoff in the background in ultraviolet-c band. The transmission peak can be shifted in the range of 251-260 nm by changing the pH value of the solution and selecting solution with a different polarity. By analyzing the spectra of the material in acid we found that the two original absorption peaks disappeared, and a new strong absorption peak appeared at 290 nm. Moreover, it seems to be some relation between the two absorption peak intensity and the new peak, which needs to be further studied. Using this result, we can produce high quality UV-filters of this material with traditional coating and colour glass technology.
