Efficient genome editing in rice with miniature Cas12f variants.

Genome editing, particularly using the CRISPR/Cas system, has revolutionized biological research and crop improvement. Despite the widespread use of CRISPR/Cas9, it faces limitations such as PAM sequence requirements and challenges in delivering its large protein into plant cells. The hypercompact Cas12f, derived from Acidibacillus sulfuroxidans (AsCas12f), stands out due to its small size of only 422 amino acids and its preference for a T-rich motif, presenting advantageous features over SpCas9. However, its editing efficiency is extremely low in plants. Recent studies have generated two AsCas12f variants, AsCas12f-YHAM and AsCas12f-HKRA, demonstrating higher editing efficiencies in mammalian cells, yet their performance in plants remains unexplored. In this study, through a systematic investigation of genome cleavage activity in rice, we unveiled a substantial enhancement in editing efficiency for both AsCas12f variants, particularly for AsCas12f-HKRA, which achieved an editing efficiency of up to 53%. Furthermore, our analysis revealed that AsCas12f predominantly induces deletion in the target DNA, displaying a unique deletion pattern primarily concentrated at positions 12, 13, 23, and 24, resulting in deletion size mainly of 10 and 11 bp, suggesting significant potential for targeted DNA deletion using AsCas12f. These findings expand the toolbox for efficient genome editing in plants, offering promising prospects for precise genetic modifications in agriculture. Supplementary Information: The online version contains supplementary material available at 10.1007/s42994-024-00168-2.

Assessing hedonic hunger among Chinese adults using the Power of Food Scale: Psychometric properties and cross-cultural invariance between China and the US.

Hedonic hunger refers to food consumption for pleasure without biological energy deficits. The Power of Food Scale (PFS) is a well-developed self-report instrument assessing hedonic hunger. The present study aimed to translate and validate the PFS into simplified Chinese (C-PFS) and examine its psychometric properties among Chinese adults. A total of 773 participants (51.1 % men, M age = 24.98 years, SD = 6.10) were recruited in the present study from college and community populations. Consistent with the previous studies, confirmatory factor analysis showed that the C-PFS had three factors: food present, food available, and food tasted. In addition, a Cronbach's alpha of 0.92 and an ICC of 0.86 suggested that the C-PFS has good internal consistency and test-retest reliability. In terms of convergent validity, the scores of C-PFS correlated significantly with disordered eating symptomatology and loss of control over eating. Measurement invariance tests showed that the C-PFS was invariant across gender and sample source groups in the Chinese sample. In addition, a U.S. sample of 490 college students (26.6 % men, M age = 21.41 years, SD = 5.45) was used to test the measurement invariance across countries, and results suggested a partial invariance across college students from China and those from the U.S. In conclusion, the C-PFS can be a useful tool for measuring hedonic hunger among adults in China, and there may be cultural differences in the measurement of the PFS in college students across China and the U.S.