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J Mol Biol ; 417(3): 165-78, 2012 Mar 30.
Article in English | MEDLINE | ID: mdl-22300764

ABSTRACT

Calcineurin is a Ca(2+)/calmodulin-dependent protein phosphatase involved in calcium signaling pathways. In Caenorhabditis elegans, the loss of calcineurin activity causes pleiotropic defects including hyperadaptation of sensory neurons, hypersensation to thermal difference and hyper-egg-laying when worms are refed after starvation. In this study, we report on arrd-17 as calcineurin-interacting protein-1 (cnp-1), which is a novel molecular target of calcineurin. CNP-1 interacts with the catalytic domain of the C. elegans calcineurin A subunit, TAX-6, in a yeast two-hybrid assay and is dephosphorylated by TAX-6 in vitro. cnp-1 is expressed in ASK, ADL, ASH and ASJ sensory neurons as TAX-6. It acts downstream of tax-6 in regulation of locomotion and egg-laying after starvation, ASH sensory neuron adaptation and lysine chemotaxis, that is known to be mediated by ASK neurons. Altogether, our biochemical and genetic evidence indicates that CNP-1 is a direct target of calcineurin and required in stimulated egg-laying and locomotion after starvation, adaptation to hyperosmolarity and attraction to lysine, which is modulated by calcineurin. We suggest that the phosphorylation status of CNP-1 plays an important role in regulation of refed stimulating behaviors after starvation and attraction to amino acid, which provides valuable nutritious information.


Subject(s)
Caenorhabditis elegans Proteins/metabolism , Caenorhabditis elegans/physiology , Calcineurin/metabolism , Carrier Proteins/metabolism , Locomotion/physiology , Oviposition/physiology , Adaptation, Physiological , Amino Acid Sequence , Animals , Animals, Genetically Modified , Arrestin/chemistry , Caenorhabditis elegans Proteins/genetics , Calcineurin/genetics , Carrier Proteins/genetics , Female , Food , Gene Expression Regulation , Lysine , Molecular Sequence Data , Mutation , Nervous System/growth & development , Phosphorylation , Sensation , Sensory Receptor Cells , Sequence Homology, Amino Acid , Two-Hybrid System Techniques
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