daf-12 encodes a nuclear receptor that regulates the dauer diapause and developmental age in C. elegans.

The daf-12 gene acts at the convergence of pathways regulating larval diapause, developmental age, and adult longevity in Caenorhabditis elegans. It encodes a nuclear receptor most closely related to two C. elegans receptors, NHR-8 and NHR-48, Drosophila DHR96, and vertebrate vitamin D and pregnane-X receptors. daf-12 has three predicted protein isoforms, two of which contain DNA- and ligand-binding domains, and one of which contains the ligand-binding domain only. Mutations cluster in DNA- and ligand-binding domains, but correspond to distinct phenotypic classes. DAF-12 is expressed widely in target tissues from embryo to adult, but is upregulated during midlarval stages. In the adult, expression persists in nervous system and somatic gonad, two tissues that regulate adult longevity. We propose that DAF-12 integrates hormonal signals in cellular targets to coordinate major life history traits.

Evanescent coupling in magneto-optical and phase-change disk systems based on the solid immersion lens.

Results of numerical computations pertaining to evanescent wave coupling for near-field magneto-optical and phase-change disks based on the concept of the solid immersion lens are presented. We investigated the relation between the coupling efficiency and the width of the air gap in terms of the throughput of the recording process and the resolution of the readout signal. The simulations show a drastic decrease with a widening air gap of the coupling efficiency by means of evanescent waves into the recording medium. In magneto-optical readout, loss of the signal may be attributed to the reduction of magneto-optical interaction, the rise of reflectance, and the variation of the relative phase between the two components of polarization. In the phase-change readout the reduced reflectivity contrast between crystalline and amorphous marks is the cause of signal reduction.

Computation of effective groove depth in an optical disk with vector diffraction theory.

Results of vector diffraction simulations pertaining to the effective groove depth for various disks with different groove parameters, different coatings, and different incident polarizations are presented. The effective depth deviates from the physical depth if the track pitch approaches the wavelength of the light source. Moreover, the difference of the effective depth for the two polarization states is demonstrated. The effective depth is usually shallower than the physical depth, especially for deeper grooves. The ray-bending mechanism associated with the objective lens and the different response to s- and p-polarized light on reflection from the disk surface impact the effective depth for objective lenses with different numerical apertures.

Isolation of csm1 encoding a class V chitin synthase with a myosin motor-like domain from the rice blast fungus, Pyricularia oryzae.

A gene encoding a chitin synthase with a myosin motor-like domain (csm1) was isolated from Pyricularia oryzae using a PCR fragment amplified from a fungal chitin synthase conserved region. The deduced amino acid sequence of csm1 is homologous to that of CsmA of Aspergillus nidulans (65% identity). The putative gene product of csm1 is consisted of the myosin motor-like domain and a chitin synthase domain as in A. nidulans csmA. The chitin synthase domain of its C-terminus was also homologous to Aspergillus fumigatus ChsE (61.4% identity) and Ustilago maydis Chs6 (48.6% identity) that encode class V chitin synthases. Northern analysis demonstrated that the csm1 was expressed throughout the mycelial growth of P. oryzae. This is the first report on the isolation of the gene encoding a class V chitin synthase with the myosin motor-like domain from P. oryzae.

Ring-toric lens for focus-error sensing in optical data storage.

We discuss the design and performance of diffractive ring-toric lenses for focus-error sensing in optical data storage. A ring-toric lens images a point source of light to a ring-shaped image. Focus-error sensing is accomplished by means of monitoring the change in ring radius: The ring expands in response to a diverging wave front, and the ring contracts in response to a converging wave front. We describe the use of a segmented phi detector to generate a focus-error signal (FES). We found that the FES slope, a measure of sensitivity to disk defocus, is higher for the ring-toric lenses described in this paper than for other techniques such as the astigmatic and the obscuration methods. We measured an FES slope of 0.7 per micrometer of disk defocus (microm(-1)). The corresponding theoretical FES slope is 0.96 microm(-1).

Polarization microscopy of magnetic domains for magneto-optical disks.

Polarization microscopes are widely used to image the magnetic domains of a magneto-optical disk and to characterize the birefringence of the disk substrate. For high-resolution imaging, unfortunately, the coupling of the polarization rotation from the Kerr signal, the effect of Fresnel's reflection coefficients, and the substrate birefringence severely deteriorate the image contrast obtained from conventional observations. Here we present the technique of differential polarization microscopy, which replaces the analyzer with a Wollaston prism, for providing better image contrast. Images of a magnetic pattern obtained with both conventional and differential methods are observed for objective lenses that have different numerical apertures and magneto-optical disks with and without a birefringent substrate. The computer simulations and experimental results show that the use of this differential method improves the image contrast and provides excellent tolerance for defects of the optical system.

Measurements and simulations of differential phase-tracking signals in optical disk data storage.

The tracking-error signal generated in differential phase detection (DPD) is theoretically analyzed and numerically simulated. Experimental measurements of the DPD signal versus the tracking offset obtained on compact read-only and phase-change disks are also reported. The signal is sensitive to the geometry of the marks, intersymbol interference along the track, and cross-track cross talk. A characteristic parameter is introduced to relate the DPD signal to the reflectivities of the mark and the spacer. For read-only disks such as CD-ROM and DVD-ROM, the magnitude of the DPD signal does not seem to depend on the reflectivity of the disks, nor does it depend on the pit depth. As for the influence of the various aberrations on the DPD signal, coma in the cross-track direction is shown to give rise to significant tracking offset, whereas defocus and spherical aberrations reduce the magnitude of the DPD signal appreciably.

Vector diffraction and polarization effects in an optical disk system.

The track pitch of current optical disks is comparable with the wavelength of the laser source. In this domain of the pitch-to-wavelength ratio, the complex-diffraction amplitudes are different for different incident polarization states, and the validity of the scalar diffraction theory is questionable. Furthermore, the use of multilayer coatings and high-numerical-aperture beams in modern optical disk technology inevitably entails the excitation of surface waves, which can disturb the baseball pattern significantly. To describe the interaction of a focused beam with a grooved multilayer system fully, it is necessary to have a rigorous vector theory. We use a rigorous vector theory to model the diffraction of light at the optical disk. We present the simulation and the experimental results and demonstrate the ability of this approach to predict or model accurately all essential features of beam-disk interaction, including the polarization effects and the excitation of surface waves.

Mutations affecting the chemosensory neurons of Caenorhabditis elegans.

We have identified and characterized 95 mutations that reduce or abolish dye filling of amphid and phasmid neurons and that have little effect on viability, fertility or movement. Twenty-seven mutations occurred spontaneously in strains with a high frequency of transposon insertion. Sixty-eight were isolated after treatment with EMS. All of the mutations result in defects in one or more chemosensory responses, such as chemotaxis to ammonium chloride or formation of dauer larvae under conditions of starvation and overcrowding. Seventy-five of the mutations are alleles of 12 previously defined genes, mutations which were previously shown to lead to defects in amphid ultrastructure. We have assigned 20 mutations to 13 new genes, called dyf-1 through dyf-13. We expect that the genes represented by dye-filing defective mutants are important for the differentiation of amphid and phasmid chemosensilla.

Secretory activity of the intraocular homotransplanted pars intermedia of the frog, Rana nigromaculata.

To examine whether there is a possible inhibitory effect of melanophore-stimulating hormone (MSH) on the release of MSH from the pars intermedia, two groups of frogs, Rana nigromaculata, were prepared with median eminence extirpation to eliminate the inhibitory hypothalamic control over MSH secretion. In one of the two groups, the animal's own intermediate lobe was transplanted to the anterior chamber of the eye (intraocular autotransplanted gland), and in the second, the animal's own pars intermedia was left in situ, and another pars intermedia was homotransplanted intraocularly. The MSH content of the homotransplanted pars intermedia was usually much increased and the number of secretory granules augmented in the secretory cells in comparison with the autotransplanted gland. These results lead to the conclusion that the release of MSH from the intraocular pars intermedia is inhibited by the in situ pars intermedia of host animals and indicate that MSH acts directly at the pituitary level to inhibit its further release, thus supporting the concept of mass action-type feedback control.