ABSTRACT
Crop impaction and candidiasis was encountered in young, meat-type pigeons (hybrid line). Prominent lesions were epithelial hyperplasia, severe hydropic degeneration and microvesicular formation in the crop. There was tissue invasion by candida blastospores and pseudohyphae. The cause was attributed to hand-feeding too much unsuitable feed at too early an age. Similar lesions were induced in an experimental study.
ABSTRACT
A DNA-binding protein has been purified from nuclei of 3T3 cells infected with polyoma virus. The assay used to detect this activity measures the amount of double-stranded DNA retained on a nitrocellulose membrane filter in the presence of binding protein. The interaction between DNA and protein is salt dependent and occurs optimally at 0.8 M NaCl. The isolated protein can bind to both circular and linear duplex DNA. Incubation of the binding protein with PM2 or polyoma DNA results in the formation of a fast sedimenting DNA structure in neutral sucrose gradients. The isolated binding protein is also capable of producing a considerable stimulation of both Escherichia coli (Pol I) and T4 DNA polymerase activities when either single-stranded or intact, native T7 DNA is used as the template. The binding protein itself is free of detectable DNA polymerase or nuclease activity.
