Evaluation of a wet processing strategy for mixed phumdi biomass conversion to bioethanol.

Biorefineries typically use dry feedstock due to technical and logistic issues, but in unique cases where climatic conditions are unfavorable and where the biomass has to be processed without a holding time, wet processing might be advantageous. The present study evaluated the possibility of using the fresh (non-dried) mixed biomass harvested from Phumdis; which are floating vegetation unique to Loktak lake in Manipur, India, for bioethanol production. Pretreatment with dilute alkali (1.5% at 120 °C for 60 min) resulted in 36% lignin removal and an enhancement of cellulose content to 48% from 37%, and enzymatic hydrolysis released 25 g/L glucose. Fermentation of the hydrolysates was highly efficient at 95%, attained in 36 h and 80% in just 12 h. The new wet processing strategy could help in value addition of mixed phumdi biomass.