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1.
Lupus ; 21(6): 649-55, 2012 May.
Article in English | MEDLINE | ID: mdl-22311939

ABSTRACT

BACKGROUND: Patients with systemic lupus erythematosus (SLE) may require prolonged periods of corticosteroid therapy which lead to excessive weight gain and increased cardiovascular risk. OBJECTIVE: To assess the utility of a low glycaemic index diet in patients with corticosteroid dependent SLE in achieving weight loss and improving glycaemic control. DESIGN: A total of 23 women were enrolled in a 6 week study. All had mild, stable SLE, were receiving corticosteroids and had a body mass index > 25 kg/m(2). Subjects were randomly assigned to a low glycaemic index (Low GI) diet or a calorie restricted (Low Cal) diet. The primary end point was weight loss. Secondary end points included tolerability of diet, bio-markers of cardiovascular risk, disease activity, fatigue and sleep quality. RESULTS: Weight loss in both treatment groups was significant (mean ± SD: Low GI diet 3.9 ± 0.9 kg; Low Cal diet 2.4 ± 2.2 kg, p < 0.01 from baseline in each group). There were also significant improvements in waist and hip measurements. However, the difference in weight loss and waist and hip measurements between the two diet groups was not statistically significant. There was a statistically significant reduction in Fatigue Severity Scale in both diet groups, (p < 0.03). Both Low GI and Low Cal diets were well tolerated, resulting in no serious adverse effects or increase in disease activity. CONCLUSION: Significant weight loss is achievable over 6 weeks in a diet-specific trial in subjects with stable SLE, who are on low dose prednisolone. Both diets were equally tolerable, and did not cause flares in disease activity. Our results suggest that dietary manipulation may significantly improve fatigue in subjects with SLE.


Subject(s)
Adrenal Cortex Hormones/therapeutic use , Caloric Restriction , Diet, Carbohydrate-Restricted , Fatigue/prevention & control , Glycemic Index , Lupus Erythematosus, Systemic/diet therapy , Lupus Erythematosus, Systemic/drug therapy , Weight Loss/physiology , Adolescent , Adrenal Cortex Hormones/adverse effects , Adrenal Cortex Hormones/pharmacology , Adult , Aged , Caloric Restriction/adverse effects , Cardiovascular Diseases/epidemiology , Cardiovascular Diseases/prevention & control , Combined Modality Therapy , Diet, Carbohydrate-Restricted/adverse effects , Dietary Carbohydrates/pharmacology , Energy Intake/physiology , Fatigue/physiopathology , Female , Humans , Lupus Erythematosus, Systemic/physiopathology , Middle Aged , Risk Factors , Severity of Illness Index , Sleep/physiology , Weight Gain/drug effects , Weight Gain/physiology , Weight Loss/drug effects , Young Adult
2.
Brain Res Bull ; 85(6): 368-73, 2011 Jul 15.
Article in English | MEDLINE | ID: mdl-21624438

ABSTRACT

Clasmatodendrosis is an irreversible astroglial degenerative change, which includes extensive swelling and vacuolization of cell bodies and disintegrated and beaded processes. Since alteration in F-actin level influences on the formation of vacuoles/vesicles during exocytosis/endocytosis in astrocytes, we investigated whether F-actin polymerization involves clasmatodendrosis in the rat hippocampus following status epilepticus (SE). In the present study, vacuoles in clasmatodendrotic astrocytes showed LAMP-1 and LC3-II (a marker for autophagy) immunoreactivity. These findings reveal that clasmatodendrosis may be lysosome-derived autophagic astroglial death. Jasplakinolide (an F-actin stabilizer) infusion significantly decreased the size and the number of medium/large-sized vacuoles in each clasmatodendritic astrocyte accompanied by enhancement of phalloidin signals, as compared to vehicle-infusion. In contrast, latrunculin A (an F-actin-depolymerizing agent) infusion increased the size and the number of medium/large-sized vacuoles, which were dissociated adjacent to cell membrane. Therefore, our findings suggest that F-actin stabilization may inhibit lysosome-derived autophagic astroglial death during clasmatodendrosis.


Subject(s)
Actins/metabolism , Astrocytes/metabolism , Astrocytes/pathology , Autophagy/physiology , Cell Death/physiology , Lysosomes/metabolism , Animals , Antineoplastic Agents/pharmacology , Astrocytes/cytology , Astrocytes/drug effects , Biomarkers/metabolism , Bridged Bicyclo Compounds, Heterocyclic/pharmacology , Depsipeptides/pharmacology , Male , Rats , Rats, Sprague-Dawley , Seizures/chemically induced , Seizures/pathology , Thiazolidines/pharmacology
3.
Neuroscience ; 187: 93-102, 2011 Jul 28.
Article in English | MEDLINE | ID: mdl-21550382

ABSTRACT

Although nuclear factor-kappa B (NF-κB) is essential for neuron survival and its activation may protect neuron against oxidative-stresses or ischemia-induced neurodegeneration, NF-κB activation can contribute to inflammatory reaction and apoptotic cell death after brain injury and stroke. However, there are little data concerning the specific pattern of NF-κB phosphorylations in neuronal damage/survival induced by status epilepticus (SE). In the present study, NF-κB phosphorylation showed the cellular specific pattern in responses to SE. p52-S865, p52-Ser869, p65-Ser276, p65-Ser311, p65-Ser468, and p65-Ser529 NF-κB phosphorylation was significantly decreased in the CA1 and CA3 pyramidal cells vulnerable to SE, although neuronal specific nuclear antigen immunoreactivity was strongly detected. In contrast, p65-Ser536 NF-κB phosphorylation was enhanced in these neurons accompanied by TUNEL- and Fluoro-Jade B 244signals. These findings serve as the first comprehensive description of the cellular specific distribution of NF-κB phosphorylation in response to pilocarpine-induced SE in the rat hippocampus, and suggest that enhancement in p65-Ser536 NF-κB phosphorylation may be closely relevant to neuronal vulnerability to SE, while others may be involved in neuronal survival.


Subject(s)
Hippocampus/metabolism , NF-kappa B/metabolism , Nerve Degeneration/metabolism , Neurons/metabolism , Status Epilepticus/metabolism , Transcription Factor RelA/metabolism , Animals , Disease Models, Animal , Immunohistochemistry , In Situ Nick-End Labeling , Male , Phosphorylation , Rats , Rats, Sprague-Dawley
4.
J Neuroimmunol ; 234(1-2): 93-102, 2011 May.
Article in English | MEDLINE | ID: mdl-21481949

ABSTRACT

Although fractalkine is one of chemokines involved in mediation of neuronal/microglial interaction, it is not known whether fractalkine/CX3CR1-mediated pathogenesis occurs in the rat brain following epileptogenic insults. In order to elucidate the roles of the fractalkine/CX3CR1 system in microglial activation and neurodegeneration induced by status epilepticus (SE), we investigated changes in fractalkine/CX3CR1 system within the rat hippocampus following SE. In non-SE induced animals, fractalkine and CX3CR1 immunoreactivity was detected in neurons and microglia, respectively. Following SE, fractalkine immunoreactivity was transiently increased in neurons and astrocytes. CX3CR1 immunoreactivity was also transiently detected in neurons (particularly in CA1 pyramidal cells). Intracerebroventricular infusions of recombinant rat fractalkine aggravated SE-induced neuronal damage, while fractalkine IgG or CX3CR1 IgG infusion alleviated it, compared to saline-infused animals. These findings suggest that fractalkine/CX3CR1 system may play an important role in SE-induced neuronal damages via neuron-microglial interactions.


Subject(s)
Chemokine CX3CL1/metabolism , Neurons/pathology , Pilocarpine , Receptors, Chemokine/metabolism , Status Epilepticus/chemically induced , Status Epilepticus/pathology , Animals , CX3C Chemokine Receptor 1 , Cell Count/methods , Chemokine CX3CL1/immunology , Disease Models, Animal , Fluoresceins , Glial Fibrillary Acidic Protein/metabolism , Glycoproteins/metabolism , Hippocampus/drug effects , Hippocampus/pathology , Immunoglobulin G/administration & dosage , Injections, Intraventricular/methods , Lectins/metabolism , Neurons/drug effects , Organic Chemicals , Rats , Rats, Sprague-Dawley , Receptors, Chemokine/immunology , Time Factors , Versicans
5.
J Neurosci Res ; 88(11): 2469-82, 2010 Aug 15.
Article in English | MEDLINE | ID: mdl-20623543

ABSTRACT

To confirm the roles of D-serinergic gliotransmission in epilepsy, we investigated the relationship between spatiotemporally specific glial responses and the D-serine/serine racemase system in mesial temporal structures following status epilepticus (SE). In control animals, D-serine and serine racemase immunoreactivities were detected mainly in astrocytes. After SE, D-serine and serine racemase immunoreactivities were increased in astrocytes. Double-immunofluorescence study revealed that up-regulation of serine racemase immunoreactivity was relevant not to D-serine immunoreactivity but to nestin or vimentin immunoreactivity. Neither D-serine nor serine racemase was found in naïve or reactive microglia. In addition, phosphorylated N-methyl-D-aspartate (NMDA) receptor subunit 1 (pNR1-Ser896) immunoreactivity in the hippocampus was increased compared with controls. Increased D-serine immunoreactivity showed direct correlation with the phosphorylation of Ser896 of NR1. Given the findings of our previous study, these findings suggest that D-serine and serine racemase in astrocytes may play roles in neuronal hyperexcitability via a cooperative activation of NMDA receptors. Furthermore, serine racemase may be involved in migration and differentiation of immature astrocytes, which is relevant to reactive astrogliosis.


Subject(s)
Epilepsy, Temporal Lobe/metabolism , Racemases and Epimerases/physiology , Serine/physiology , Animals , Astrocytes/metabolism , Cell Movement/physiology , Electrophysiology , Epilepsy, Temporal Lobe/enzymology , Fluorescent Antibody Technique , Glial Fibrillary Acidic Protein/metabolism , Hippocampus/cytology , Hippocampus/enzymology , Hippocampus/metabolism , Immunohistochemistry , Neuroglia/enzymology , Neuroglia/metabolism , Rats , Rats, Sprague-Dawley , Receptors, N-Methyl-D-Aspartate/metabolism , Seizures/metabolism , Seizures/physiopathology
6.
Neuroscience ; 163(3): 781-9, 2009 Oct 20.
Article in English | MEDLINE | ID: mdl-19619613

ABSTRACT

In order to elucidate the roles of aquaporins (AQPs) in astroglial responses, we investigated AQP expressions in the experimental epileptic hippocampus. In control animals, AQP1 protein expression was restricted to the ventricular-facing surface of the choroid plexus. AQP4 was expressed in astrocyte foot processes near blood vessels and in ependymal and pial surfaces in contact with cerebrospinal fluid. AQP9 protein has been detected in cells lining the cerebral ventricles, and in astrocytes. Six to eight weeks after status epilepticus (SE), AQP1 expression was mainly, but not all, detected in vacuolized astrocytes, which were localized in the stratum radiatum of the CA1 region. AQP4 was negligible in vacuolized CA1 astrocytes, although AQP4 immunoreactivity in non-vacuolized astrocytes was increased as compared to control level. AQP9 expression was shown to be mainly induced in non-vacuolized CA1 astrocytes. Therefore, our findings suggest that AQP subunits may play differential roles in various astroglial responses (including astroglial swelling and astroglial loss) in the chronic epileptic hippocampus.


Subject(s)
Aquaporins/biosynthesis , Astrocytes/metabolism , Epilepsy/metabolism , Hippocampus/metabolism , Animals , Aquaporin 1/biosynthesis , Aquaporin 4/biosynthesis , Chronic Disease , Immunohistochemistry , Male , Protein Subunits/biosynthesis , Rats , Rats, Sprague-Dawley , Status Epilepticus/metabolism
7.
Clin Exp Rheumatol ; 17(6 Suppl 18): S8-12, 1999.
Article in English | MEDLINE | ID: mdl-10589351

ABSTRACT

The concept of combination therapy implies the concurrent use of two or more slow-acting antirheumatic drugs to treat rheumatoid arthritis. This review places such combination therapy into an historical context and evaluates studies carried out before 1990. There were no published studies before 1980 of combination therapy, and between 1980 and 1990 there were 11 published studies. Three were conventional randomised controlled trials, three were non-randomised studies of parallel group design, four were observational open studies, and one was a retrospective review. Altogether 486 patients were studied with the numbers of cases in each study varying between 12 and 101. The main combinations used were penicillamine + hydroxychloroquine or chloroquine, gold + hydroxychloroquine, and sulphasalzine + penicillamine. Six studies concluded that combination therapy helped patients, three suggested possible benefits, and two gave essentially negative findings. These two negative studies were randomised controlled trials. Most studies indicated an increase in adverse events with combination therapy. The average erythrocyte sedimentation rate on combination therapy fell by 21.4%. A majority of patients remained on the therapy for 6-12 months. The balance of evidence in 1990 suggested that combination therapy had a modest advantage. However, the trials were too small to detect its true value, and the combinations used were not ideal. In particular, combining gold or penicillamine with other drugs appeared to give too much toxicity. The future development of the field would depend on identifying more effective combinations of drugs and undertaking larger and better-designed trials.


Subject(s)
Antirheumatic Agents/therapeutic use , Arthritis, Rheumatoid/drug therapy , Drug Therapy, Combination , Humans , Time Factors
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