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1.
Clin Oral Implants Res ; 17(3): 337-44, 2006 Jun.
Article in English | MEDLINE | ID: mdl-16672031

ABSTRACT

OBJECTIVE: The aim of this study was to analyze long-term implant survival in the mandible after radiotherapy and radical surgery in oral cancer patients. STUDY DESIGN: Between 1990 and 2003, 71 patients (15 females, 56 males; average age 57.8 years, range 16-84.1 years) were treated with dental implants after radiochemotherapy and ablative surgery of oral cancer. Radiation therapy was delivered in daily fractions of 2 Gy given on 25 days (total dose of 50 Gy). Oral defects were reconstructed microsurgically with jejunal, iliac crest or radial forearm grafts. Thereafter 316 dental implants were placed in the non-irradiated residual bone (84; 27%), irradiated residual bone (154; 49%) or grafted bone (78; 25%) at various intervals (mean interval 1.41 (+/- 1.01) years, range 0.34-6.35 years). RESULTS: The mean follow-up time after implant insertion was 5.42 (+/- 3.21) years (range 0.3-13.61 years). The overall 2-, 3-, 5-, and 8-year survival rates of all implants were 95%, 94%, 91% and 75%. Forty-four implants were lost in 21 patients during the observation period. Irradiation of the mandibular bone showed significantly (P = 0.0028) lower implant survival compared with non-irradiated mandibular bone. The 8-year survival rate in the non-irradiated residual bone (two loss), irradiated residual bone (29 loss) or grafted bone (13 loss) were 95%, 72% and 54%, respectively. Time of implantation after irradiation showed no statistically significant influence. Implant brand, length or diameter or the incidence of resective surgery on the mandible and gender of patients had no statistically significant influence on implant survival. CONCLUSION: Radiation therapy with 50 Gy was significantly related to shorter implant survival in mandibular bone. Survival was lowest in grafted bone. Time of implant placement had no statistically significant influence on survival under the conditions of this study. Although implant survival is lower in irradiated mandibles, implants significantly facilitate prosthodontic treatment and enhance outcome of oral rehabilitation in cancer patients.


Subject(s)
Dental Implants , Mandible/surgery , Mouth Neoplasms/radiotherapy , Adolescent , Adult , Aged , Aged, 80 and over , Bone Transplantation , Dental Implantation, Endosseous , Dental Prosthesis Design , Dental Restoration Failure , Dose Fractionation, Radiation , Female , Follow-Up Studies , Humans , Longitudinal Studies , Male , Mandible/radiation effects , Microsurgery , Middle Aged , Mouth Neoplasms/drug therapy , Mouth Neoplasms/surgery , Neoadjuvant Therapy , Plastic Surgery Procedures , Survival Analysis , Treatment Outcome
2.
Article in English | MEDLINE | ID: mdl-16545702

ABSTRACT

OBJECTIVE: Biodegradable materials are particularly useful for the fixation of zygomatic fractures. Different systems are commercially available. The aim of this study was to compare the clinical outcome of zygomatic fracture fixation using 3 biodegradable systems and a titanium osteosynthesis system. STUDY DESIGN: Patients with displaced fractures of the zygomatic bone presenting at our department from October 2001 to May 2003 were randomly allocated to 1 of 3 treatment groups for fracture fixation (study group A: LactoSorb: n = 18; study group B: BioSorb: n = 18; study group C: Delta: n = 18). Treatment outcome and complication rates were compared with a historic patient group with zygomatic fractures fixed with titanium osteosynthesis (control group D: n = 15). RESULTS: A total of 64 patients (study groups A + B + C: n = 49; control group D: n = 15) were followed for at least 24 months (range: 24 to 44 months). Forty-nine patients in the biodegradable study groups (group A: n = 15; group B: n = 17; group C: n = 17) who had their fractures fixed with biodegradable plates and screws alone or in combination with titanium plates and screws were reviewed postoperatively. Uneventful healing occurred during the entire follow-up period in 39 (80%) out of 49 patients in the biodegradable groups (A + B + C) and in 12 (80%) out of 15 patients in group D. Ten patients in groups A + B + C developed postoperative complications (infection: n = 3; soft tissue dehiscence: n = 2; implant-related tissue reactions: n = 5), compared with 3 patients in group D (soft tissue dehiscence: n = 1; unspecific pain: n = 2) (P = .97). Complications occurred in 4 patients in group A and 3 patients each in groups B and C. Smokers developed significantly more postoperative complications than nonsmokers in groups A + B + C (P = .01). CONCLUSION: There was no significant difference between biodegradable osteosynthesis materials or between biodegradable materials and titanium fixation with respect to fracture healing and postoperative complications. Postoperative complications were of a minor nature and resolved spontaneously or after local therapy. Smoking habits may play a significant role in the incidence of complications with biodegradable materials.


Subject(s)
Absorbable Implants/adverse effects , Biocompatible Materials/adverse effects , Fracture Fixation, Internal/adverse effects , Zygomatic Fractures/surgery , Adolescent , Adult , Aged , Bone Plates/adverse effects , Bone Screws/adverse effects , Female , Foreign-Body Reaction/etiology , Fracture Fixation, Internal/instrumentation , Fracture Healing , Humans , Lactic Acid/adverse effects , Male , Middle Aged , Pain, Postoperative/etiology , Polyglycolic Acid/adverse effects , Polylactic Acid-Polyglycolic Acid Copolymer , Polymers/adverse effects , Prospective Studies , Smoking/adverse effects , Surgical Wound Dehiscence/etiology , Surgical Wound Infection/etiology , Titanium
3.
J Oral Maxillofac Surg ; 64(3): 550-9, 2006 Mar.
Article in English | MEDLINE | ID: mdl-16487823

ABSTRACT

PURPOSE: To evaluate the feasibility and accuracy of a new method for planning and realizing zygomatic osteotomies in cases of established post-traumatic deformities using stereolithographic (SL) models and computer assisted navigation. PATIENTS AND METHODS: In 5 patients, osteotomy and repositioning of the zygomatic complex was planned using SL models. The desired position of the zygoma in the patient was determined by fixing individualized osteosynthesis plates to predefined screw positions. The SL model and the patient were registered to the same 3-dimensional computed tomography data set via an occlusal reference frame on the patient and corresponding reference markers on the model. Prebent osteosynthesis plates from the surgical simulation on the model were fixed to corresponding screw positions on the patient, which were located by computer-assisted navigation. Evaluation of accuracy was performed by image fusion of postoperative computed tomography scans of the model and the patient. RESULTS: Clinical outcome was satisfactory in all cases. The evaluation by image fusion showed alignment of the patient's and the model's zygoma in 4 of 5 cases. Mean measured distance between screw positions in the models and the patients were 1.1 +/- 0.3 mm for 44 screws. In 1 patient the treatment plan was changed intraoperatively because of unforeseen soft tissue limitations. CONCLUSION: Point-to-point navigation is an accurate method to transfer the planning of a complex osteotomy from the SL model to the actual surgical procedure. Surgery is facilitated considerably because repositioning and osteosynthesis are achieved in 1 step.


Subject(s)
Facial Asymmetry/surgery , Imaging, Three-Dimensional/methods , Osteotomy/methods , Surgery, Computer-Assisted/methods , Zygomatic Fractures/surgery , Adult , Facial Asymmetry/diagnostic imaging , Facial Asymmetry/etiology , Female , Humans , Male , Middle Aged , Models, Anatomic , Patient Care Planning , Tomography, X-Ray Computed , Treatment Outcome , Zygoma/diagnostic imaging , Zygoma/injuries , Zygoma/surgery , Zygomatic Fractures/complications , Zygomatic Fractures/diagnostic imaging
4.
Clin Oral Implants Res ; 16(4): 417-24, 2005 Aug.
Article in English | MEDLINE | ID: mdl-16117765

ABSTRACT

Bone tissue engineering is a promising approach for treatment of defective and lost bone in the maxillofacial region. Creating functional tissue for load bearing bone reconstruction using biocompatible and biodegradable scaffolds seeded with living cells is of crucial importance. The aim of our study was to compare the effects of poly-lactic-co-glycolic acid (PLGA) and hydroxyapatite (HA) ceramic granulae on growth, differentiation, mineralization and gene expression of mandibular mesenchymal cambial layer precursor cells (MCLPCs) cultured onto tissue engineered three-dimensional (3-D) composites in vitro. These 3-D composites were cultivated in a rotating cultivation system under osteogenic differentiation conditions for a maximum period of 21 days. After 6 and 21 days, histological examination was performed; scanning electron microscopy (SEM), alkaline phosphatase (ALP) activity and levels of DNA were investigated. Expression of bone-specific genes osteocalcin, osteonectin, osteopontin, ALP, core binding factor alpha 1 and collagen type I were investigated by using a reverse transcription-polymerase chain reaction (RT-PCR) method. After 6 and 21 days of incubation an initiation of mineralization and the presence of newly formed bone at the surface of the composites were shown after evaluation of ALP activity, DNA content, SEM and histological staining. Expression of bone-specific genes confirmed the bone-like character of these composites and different effects of PLGA or HA granulae on the osteogenic differentiation of human MCLPCs in vitro. The results of this study support the concept that substrate signals significantly influence MCLPCs growth, differentiation, mineralization and gene expression in vitro, and that the use of these cells in the manufacturing of 3-D cell/HA composites is a promising approach for load bearing bone reconstruction in the maxillofacial region in vivo.


Subject(s)
Biocompatible Materials/chemistry , Mesenchymal Stem Cells/physiology , Tissue Engineering/methods , Alkaline Phosphatase/analysis , Alveolar Ridge Augmentation/methods , Bone Substitutes/chemistry , Calcification, Physiologic , Cell Differentiation , Cell Division , Cells, Cultured , Collagen Type I/analysis , Core Binding Factor alpha Subunits , DNA-Binding Proteins/analysis , Durapatite/chemistry , Humans , Lactic Acid/chemistry , Maxillary Sinus/surgery , Osteocalcin/analysis , Osteogenesis/physiology , Osteonectin/analysis , Osteopontin , Polyglycolic Acid/chemistry , Polylactic Acid-Polyglycolic Acid Copolymer , Polymers/chemistry , Sialoglycoproteins/analysis , Time Factors , Tissue Engineering/instrumentation , Transcription Factors/analysis
5.
Plast Reconstr Surg ; 115(7): 1863-70, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15923830

ABSTRACT

BACKGROUND: Biodegradable implants have not been used on a large scale for internal fixation of mandibular fractures because of presumed inferior mechanical properties. This prospective clinical trial was designed to elucidate the stability and biocompatibility of self-reinforced poly-L/D-lactide plates and screws used to stabilize a variety of mandible fractures by open reduction and internal fixation. METHODS: Sixty-six consecutive patients (22 female, 44 male; mean age, 23.9 years) with a total of 89 fractures at various sites of the mandible were included in the study. Stability of plates and screws and bone healing were observed by clinical and radiographic assessment. Intermaxillary fixation was applied in eight patients with concomitant condylar fractures for 2 to 3 weeks. RESULTS: The self-reinforcement technique provided sufficient mechanical stability of the implants for primary healing of these high-load mandibular bone areas. Postoperative complications were transient and limited to wound dehiscence and localized wound infection (two patients). In some patients, hypesthesia (three patients) or slight pain (10 patients) was reported at the 1-year recall examination, but implant-related serious adverse tissue reactions were not observed during the follow-up (mean, 24.4 months; range 6.4 to 44.3 months). CONCLUSIONS: On the basis of these preliminary results, the authors conclude that biodegradable self-reinforced implants show efficient stability during initial bone healing and promise a high potential for successful use in osteofixation of mandibular fractures.


Subject(s)
Absorbable Implants , Bone Plates , Bone Screws , Fracture Fixation, Internal , Mandibular Fractures/surgery , Adolescent , Adult , Aged , Biomechanical Phenomena , Child , Child, Preschool , Equipment Design , Female , Fracture Healing , Humans , Male , Mandibular Fractures/diagnostic imaging , Middle Aged , Polyesters , Prospective Studies , Radiography, Panoramic
6.
Article in English | MEDLINE | ID: mdl-15953913

ABSTRACT

OBJECTIVE: The purpose of this retrospective study was to evaluate complications before, during, and after vertical alveolar distraction osteogenesis and to assess the survival rate of dental implants placed in distracted bone. STUDY DESIGN: In a consecutive series, 37 patients with 45 alveolar ridge deficiencies of the partially edentulous mandible were treated with 14 intraosseous and 31 subperiosteal distraction devices. Seventy-two dental implants could be placed at the time of distractor removal and 21 implants at a second stage. RESULTS: Complications associated with the distraction procedure affected 75.7% of patients. The majority of complications were of minor nature with the exception of fractures of basal bone (n = 3), fracture of transport segment (n = 1), breakage of distractor (n = 1), and severe mechanical problems (n = 3). Eleven secondary grafting procedures were necessary to allow the placement of dental implants. Implant survival was 95.7% (mean postloading follow-up: 35.7 months). CONCLUSION: Vertical alveolar distraction osteogenesis is not an uncomplicated procedure; however, long-term survival of dental implants inserted into distracted areas is satisfactory.


Subject(s)
Alveolar Ridge Augmentation/methods , Jaw, Edentulous, Partially/rehabilitation , Mandible/surgery , Osteogenesis, Distraction/adverse effects , Adolescent , Adult , Aged , Alveolar Ridge Augmentation/adverse effects , Bone Transplantation , Dental Implantation, Endosseous , Dental Prosthesis, Implant-Supported , Denture, Partial, Fixed , Equipment Failure , Female , Humans , Hypesthesia/etiology , Jaw, Edentulous, Partially/surgery , Life Tables , Male , Mandibular Fractures/etiology , Middle Aged , Osteogenesis, Distraction/instrumentation , Retrospective Studies , Surgical Wound Dehiscence/etiology
7.
Article in English | MEDLINE | ID: mdl-15953912

ABSTRACT

OBJECTIVE: The aim of this study was to assess the safety and efficiency of biodegradable self-reinforced (SR-PLDLA) bone plates and screws in open reduction and internal fixation of mandible fractures in children. STUDY DESIGN: Thirteen patients (5 female, 8 male; mean age 12 years, range 5-16 years) were operated on various fractures of the mandible (2 symphyseal, 6 parasymphyseal, 4 body, 3 angle, 1 ramus, 2 condylar fractures). The mean follow-up time was 26.4 months (range 10.9-43.4 months). Intermaxillary fixation was applied in cases with concomitant condylar fractures up to 3 weeks. RESULTS: Primary healing of the fractured mandible was observed in all patients. Postoperative complications were minor and transient. The outcome of the operations was not endangered. Adverse tissue reactions to the implants, malocclusion, and growth restrictions did not occur during the observation period. CONCLUSIONS: Pediatric patients benefit from the advantages of resorbable materials, especially from faster mobilization and the avoidance of secondary removal operations. Based on these preliminary results, self-reinforced fixation devices are safe and efficient in the treatment of pediatric mandible fractures. However, further clinical investigations are necessary to evaluate the long-term reliability.


Subject(s)
Absorbable Implants , Fracture Fixation, Internal/instrumentation , Jaw Fixation Techniques/instrumentation , Mandibular Fractures/surgery , Adolescent , Bone Plates , Bone Screws , Child , Child, Preschool , Female , Humans , Lactic Acid , Male , Polyesters , Polymers
8.
J Oral Maxillofac Surg ; 63(6): 793-9, 2005 Jun.
Article in English | MEDLINE | ID: mdl-15944976

ABSTRACT

PURPOSE: The purpose of this study was to analyze the interaction between osteoblast-like cells isolated from mandibular bone and hydroxyapatite ceramic bone substitute obtained from calcified red algae to assess the growth and differentiation of adherent cells on this biomaterial. MATERIALS AND METHODS: The macroporous ceramic material C GRAFT/Algipore (The Clinician's Preference LLC, Golden, CO) is composed of 100% hydroxyapatite and possesses specific mechanical and physiochemical properties. Osteoblast-like cells were seeded on 200 mg of biomaterial and cultured for 6 and 21 days under osteogenic differentiation conditions. Specific alkaline phosphatase activity, DNA, and protein content of the proliferating cells were analyzed. The morphology of the cells in contact with the biomaterial was examined by scanning electron microscopy. The osteoblastic phenotype of the cells was confirmed by analysis of the expression of bone-specific genes (osteocalcin, osteopontin and collagen type I) by semi-quantitative reverse transcriptase polymerase chain reaction. RESULTS: The DNA and protein content increased over the culture period. Scanning electron microscopy showed cells spreading on the surface of the biomaterials, covering the macropores, and colonizing the depth of the particles. The analysis of the expression patterns of bone-related genes confirmed the osteoblastic phenotype of the cultured cells. CONCLUSION: The results of this study showed that hydroxyapatite ceramic bone substitute obtained from calcified red algae support the proliferation and differentiation of human osteoblast-like cells on its surface in vitro and might be suitable for use as scaffolds in tissue engineering strategies in vivo.


Subject(s)
Bone Substitutes , Osteoblasts/cytology , Tissue Engineering , Cell Adhesion , Cell Culture Techniques , Cell Differentiation , Cell Proliferation , Cells, Cultured , Ceramics , Collagen Type I/biosynthesis , Durapatite , Humans , Mandible/cytology , Materials Testing , Microscopy, Electron, Scanning , Osteoblasts/metabolism , Osteocalcin/biosynthesis , Osteopontin , RNA, Messenger/analysis , Reverse Transcriptase Polymerase Chain Reaction , Rhodophyta , Sialoglycoproteins/biosynthesis
9.
J Oral Maxillofac Surg ; 63(5): 673-81, 2005 May.
Article in English | MEDLINE | ID: mdl-15883943

ABSTRACT

PURPOSE: Bone tissue engineering is a promising approach for the treatment of defective or lost bone in the maxillofacial region. Biocompatible and biodegradable scaffolds seeded with living cells are used to create functional tissue for load-bearing bone reconstruction. The aim of this study was to manufacture cell-seeded 3-dimensional bone constructs based on hydroxyapatite ceramic granule calcified from red algae and mesenchymal cambial-layer precursor cells. The ability of these cells to grow on hydroxyapatite ceramic was quantitatively investigated to evaluate 3-dimensional bone constructs for their potential use in bone tissue engineering. MATERIALS AND METHODS: Mesenchymal cambial-layer precursor cells were isolated from mandibular periosteum biopsy samples of 3 patients. To manufacture 72 bone constructs, these cells and hydroxyapatite ceramic granules (C GRAFT/Algipore; Clinician's Preference LLC, Golden, CO) were cultivated under osteogenic differentiation conditions in a rotating wall vessel system. After 6 and 21 days, histologic examination and scanning electron microscopy were performed. The absolute DNA content, protein synthesis, and alkaline phosphatase activity were also quantified. The osteoblastic phenotype of the constructs was confirmed by the expression of bone-specific genes (osteocalcin, osteonectin, osteopontin, and core binding factor alpha1) using semiquantitative reverse transcription-polymerase chain reaction and Western blot analysis. RESULTS: Cells within the constructs showed good viability, which was evidenced by an increase in DNA content over the culture period. The decrease in alkaline phosphatase-specific activity could be an indicator of the maturation of cells and the induction of mineralization. The osteoblastic phenotype of the constructs was demonstrated on protein and at the RNA level over the entire culture period. CONCLUSION: We observed a positive effect of hydroxyapatite ceramic granules on mesenchymal cambial-layer precursor cell behavior in cell-seeded 3-dimensional bone constructs, indicating the potential applicability of C GRAFT/Algipore composites in bone tissue engineering.


Subject(s)
Bone Regeneration , Durapatite/pharmacology , Rhodophyta , Tissue Engineering/methods , Blotting, Western , Bone Substitutes , Cell Culture Techniques , Electrophoresis, Polyacrylamide Gel/methods , Materials Testing , Mesenchymal Stem Cells/metabolism , Microscopy, Electron, Scanning , Osteocalcin/metabolism , Osteonectin/metabolism , Osteopontin , Reverse Transcriptase Polymerase Chain Reaction/methods , Sialoglycoproteins/metabolism
10.
J Biomed Mater Res A ; 73(4): 445-55, 2005 Jun 15.
Article in English | MEDLINE | ID: mdl-15900611

ABSTRACT

The ability to generate new bone for reconstructive surgery use is a major clinical need. Tissue engineering with osteoprogenitor cells isolated from the patient's periosteum and seeded into bioresorbable scaffolds offers a promising approach to the generation of skeletal tissue. To our knowledge, there is no description about the expression of Ets2 in tissue engineered "bone neotissue." The aim of our study was to manufacture cell-seeded three-dimensional bone constructs with human periosteal cells on poly (lactic-co-glycolic acid) polymer fleeces to describe the expression pattern of Ets2 and its target genes osteocalcin and osteopontin; expression analysis of type I collagen, core-binding factor-1, alkaline phosphatase, and osteonectin; the ability of matrix mineralization and ALP enzymatic activity showed the osteogenic character of the constructs. A significant correlation between the expression of Ets2 and osteopontin mRNA (r = -0.70; p < 0.05) could be shown. A 1.35-fold increase of Ets2 expression from days 1 to 9 was detected, followed by a slight decrease from days 11 to 15. Until the end of the culture period, the expression of Ets2 reached a comparable high level as detected on day 9. In contrast, the expression level of osteopontin mRNA reached a maximum at day 7, followed by a progressive 3.04-fold decrease until day 21. This study shows for the first time that Ets2 gene and its transcriptional target genes are expressed in tissue-engineered bone constructs. These findings have the potential to provide much-needed information about the role and function of Ets2 in human osteogenesis processes and creation of "bone neotissue."


Subject(s)
Biocompatible Materials/chemistry , Bone Substitutes/chemistry , Bone and Bones/metabolism , Chromosomes, Human, Pair 21 , Proto-Oncogene Proteins/biosynthesis , Trans-Activators/biosynthesis , Alkaline Phosphatase/metabolism , Bone and Bones/pathology , Cell Proliferation , Cells, Cultured , Core Binding Factors , DNA Primers/chemistry , Gene Expression Regulation , Glycolates/chemistry , Humans , Lactic Acid/chemistry , Mesoderm/cytology , Mesoderm/pathology , Microscopy, Electron, Scanning , Neoplasm Proteins/metabolism , Osteoblasts/metabolism , Osteocalcin/chemistry , Osteocalcin/metabolism , Osteogenesis , Osteonectin/chemistry , Osteopontin , Polyglycolic Acid/chemistry , Polylactic Acid-Polyglycolic Acid Copolymer , Polymers/chemistry , Proto-Oncogene Protein c-ets-2 , RNA, Messenger/metabolism , Reverse Transcriptase Polymerase Chain Reaction , Sialoglycoproteins/chemistry , Time Factors , Tissue Engineering , Transcription Factors/metabolism
11.
J Craniomaxillofac Surg ; 33(2): 95-102, 2005 Apr.
Article in English | MEDLINE | ID: mdl-15804587

ABSTRACT

PURPOSE: The aim of this retrospective clinical study was to evaluate zygomatic fracture fixation with the BioSorbFX osteosynthesis system by assessing stability of reduction as well as complications in the first postoperative year and by conducting a survey to document surgeons' opinions on biodegradable osteosynthesis for this indication. MATERIAL: From January to September 2003, 25 patients with displaced non-infected unilateral fracture of the zygoma were operated upon (m:f = 20:5; age 17-81 years; mean 39.4 years) using the BioSorbFX 2.0 and/or 1.5 mm osteosynthesis systems. METHODS: A: Clinical and radiographic examinations were carried out immediately postoperatively and after 1, 3, 6, 9 and 12 months. METHODS: B: Surgeons were asked to participate in a survey critically evaluating their experience with biodegradable osteosynthesis systems. RESULTS: A: All fractures of the zygoma healed uneventfully. An excessive soft tissue reaction due to the degradation process was not seen. Three minor complications which resolved after local therapy occurred in the immediate postoperative phase. RESULTS: B: In the survey, the handling of biodegradable plates in general was rated worse than metal plate osteosynthesis. CONCLUSION: Fixation of fractures of the zygoma with the BioSorbFX system was simple and safe. The fixations remained stable and bony healing was uneventful. Postoperative complications were few, of a minor nature and not related to the process of biodegradation.


Subject(s)
Absorbable Implants , Fracture Fixation, Internal/instrumentation , Oral Surgical Procedures/instrumentation , Zygomatic Fractures/surgery , Adolescent , Adult , Aged , Aged, 80 and over , Bone Plates , Bone Screws , Female , Humans , Male , Middle Aged , Postoperative Care , Radiography , Retrospective Studies , Surveys and Questionnaires , Zygomatic Fractures/diagnostic imaging
12.
Int J Mol Med ; 15(2): 247-51, 2005 Feb.
Article in English | MEDLINE | ID: mdl-15647839

ABSTRACT

Van der Woude syndrome (VWS) is an autosomal dominant disorder characterized by clefts of the lip and/or palate (CL+/-P), lip pits, bifid uvula and hypodontia. Mutations of the interferon regulatory factor 6 gene (IRF6) have been recently described in patients with VWS. The entire 9 exons of the IRF6 gene in two brothers of Turkish origin clinically diagnosed with Van der Woude syndrome and four healthy family members were screened for mutations using a newly established denaturing gradient gel electrophoresis (DGGE) method. A novel heterozygous mutation in exon 2 (DNA binding region) of the IRF6 gene, p.Arg84Gly, was found in both brothers with VWS and in their clinically asymptomatic mother. Our results suggest a dominant negative effect of the p.Arg84Gly mutation in the VWS of both patients. Non-penetrance of this mutation is suggested in the mother of the patients.


Subject(s)
Abnormalities, Multiple/genetics , Anodontia/genetics , Arginine/genetics , Cleft Lip/genetics , Cleft Palate/genetics , DNA-Binding Proteins/genetics , Glycine/genetics , Mouth Abnormalities/genetics , Mutation , Transcription Factors/genetics , 5' Untranslated Regions , Base Sequence , DNA/metabolism , DNA Primers/genetics , Electrophoresis, Polyacrylamide Gel , Exons , Family Health , Female , Genes, Dominant , Heterozygote , Humans , Interferon Regulatory Factors , Male , Molecular Sequence Data , Pedigree , Penetrance , Phenotype , Promoter Regions, Genetic , Protein Structure, Tertiary , Sequence Analysis, DNA , Syndrome , Turkey
13.
J Craniomaxillofac Surg ; 32(6): 343-9, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15555515

ABSTRACT

INTRODUCTION: Lymphoscintigraphic planar imaging is a common procedure for sentinel lymph node imaging prior to lymph node biopsy, but fails to elucidate the specific lymphatic drainage. Composite functional/anatomical imaging (SPECT-CT) has the potential to enhance topographic orientation and diagnostic sensitivity of sentinel lymph node imaging, but has not yet been applied in the head and neck region. STUDY DESIGN: A total of 30 patients were investigated. Planar imaging was 5 min, 265 x 265, right and left lateral; 500 kilocounts (Kcts) and SPECT (GE Millenium VG Hawk Eye 6 degrees/30s. step, 128 x 128, slice thickness 4.42 mm). Scans were performed 60 min after intra-mucodermal injection of 0.1 ml of 20 MBq 99mTc nanocolloid in patients with squamous cell cancer of the head and neck. SPECT studies were analysed by filtered back projection (FBP: Hann (0.7) prefiltering, Butterworth (0.5) postfiltering) and reconstruction (OSEM: Post Filter Hamming (0.85), 2 Iterations) and independently viewed with the co-registered CT image (eNTEGRA Functional Anatomical Fusion Vers 2.0216). The results were validated by comparing the results of each method employed in all 30 cases and intraoperative gamma probe-guided sentinel lymph node biopsy with histological examination in 13 of these patients. RESULTS: The majority of patients had more than one sentinel node (mean 1.63, min. 0, max. 4). Seven out of the 30 studies demonstrated lymphatic flow to the contralateral side of the neck. Forty-nine sentinel nodes were identified by iteratively reconstructed SPECT-CT. Thirty-eight out of these 49 could be located in lymphoscintigraphic planar imaging, whereas only 24/49 were detected in filtered back projection, respectively. In 11 of the 30 cases, a clinically unpredictable pattern of lymphatic drainage was observed. No correlation was found between T stage or tumour location and the number of sentinel nodes detected. In one out of the 13 cases, in whom imaging was followed by intraoperative gamma probe-guided biopsy, no sentinel node could be detected with the probe in the proximity of the primary tumour, although the node was clearly discernible in the reconstructed SPECT-CT. CONCLUSION: Composite functional/anatomical imaging (SPECT-CT) is feasible for sentinel lymph node detection. It enhances topographic orientation and diagnostic sensitivity with more sentinel nodes being detectable than by planar lymphoscintigraphy alone. Planar imaging should be accompanied by iterative reconstructed SPECT-CT to identify lymph nodes adjacent to the primary lesion. Such nodes are easily overlooked by planar lymphoscintigraphy and intraoperative gamma probes, as the high activity at the injection site can obscure their detection.


Subject(s)
Carcinoma, Squamous Cell/pathology , Head and Neck Neoplasms/pathology , Lymph Nodes/pathology , Sentinel Lymph Node Biopsy/methods , Tomography, Emission-Computed, Single-Photon , Tomography, X-Ray Computed , Adult , Aged , Aged, 80 and over , Carcinoma, Squamous Cell/surgery , Feasibility Studies , Female , Gamma Cameras , Head and Neck Neoplasms/surgery , Humans , Image Processing, Computer-Assisted/methods , Lymph Node Excision , Lymphatic Metastasis , Male , Middle Aged , Mouth Neoplasms/pathology , Mouth Neoplasms/surgery , Neoplasm Staging , Oropharyngeal Neoplasms/pathology , Oropharyngeal Neoplasms/surgery , Radiopharmaceuticals , Technetium Tc 99m Aggregated Albumin
14.
Clin Oral Implants Res ; 15(6): 693-9, 2004 Dec.
Article in English | MEDLINE | ID: mdl-15533130

ABSTRACT

OBJECTIVE: The aim of this study was to determine the long-term outcome of implant insertion in the augmented severely atrophied maxilla. STUDY DESIGN: Three hundred and twenty-four implants were inserted in 35 patients (eight males, 27 females, average age 57.6 years) in extremely atrophied maxillae after osteotomy and interposition of iliac crest bone. One hundred implants were installed in 12 patients simultaneously with the osteotomy and grafting; 224 implants were placed in 23 patients in a second procedure 6-12 months later. Implant parameters like osseointegration and peri-implant bone loss; peri-implant tissue parameters like bleeding, gingival and plaque index; and patients' satisfaction were evaluated. RESULTS: Of 324 implants, 29 (8.9%) were lost during the entire follow-up: 14 in six patients of the one-step and 15 in 11 patients of the two-step group. The overall input-output survival in 141.1 months was 91.1%. The overall 2-year failure-free fraction of implants was 95.5%; the 5-year failure-free fraction was 89.3%. In the one-step group, the 2 (5)-year failure-free fraction was 95.9% (86.9%), and in the two-step group 95% (91.3%) (log-rank test P=0.57). Marginal peri-implant bone loss was 1.7+/-1.3 mm mesial and 1.8+/-1.3 mm distal. CONCLUSION: Implant insertion after osteotomy and iliac bone grafting is a reliable operation method for the dental rehabilitation of the severely atrophied maxilla showing good long-term results.


Subject(s)
Dental Implants/statistics & numerical data , Dental Restoration Failure , Atrophy/pathology , Bone Transplantation , Dental Implants/adverse effects , Female , Graft Survival , Humans , Male , Maxilla/pathology , Maxilla/surgery , Middle Aged , Osteotomy, Le Fort/statistics & numerical data , Retrospective Studies
15.
Article in English | MEDLINE | ID: mdl-15184849

ABSTRACT

OBJECTIVE: The purpose of this study was to determine the long-term outcome of the horseshoe Le Fort I osteotomy (HLFO) as a preprosthetic operation technique for implant insertion in the extremely atrophied maxilla. STUDY DESIGN: 36 patients (8 male, 28 female, average age 57.6 years) underwent HLFO combined with iliac crest bone grafting. They were divided into 2 groups: group A with 12 patients who simultaneously received 100 implants; group B with 24 patients where 176 implants were inserted in 18 patients in a second-stage procedure. Clinical and radiographic outcome with regard to implant osseointegration, alveolar bone height in the canine and molar regions, peri-implant bone loss and satisfaction of patients (esthetics, masticatory function, overall treatment) were investigated in all cases. RESULTS: The overall 2-year failure-free fraction of implants was 95.5%; the 5-year failure-free fraction was 89.3%. In the 1-step group the 2-year and 5-year failure-free fractions were 95.9% and 86.9%, respectively, in the 2-step group 95.0% and 91.3% (log rank test P=.57). A total of 27 implants were lost during the entire follow-up: 14 in 6 patients of the 1-stage and 13 in 9 patients of the 2-stage group. The mean loss of alveolar bone after augmentation in the canine and molar regions was almost equal in both groups (overall means for the 2 regions 3.67 +/- 2.77 and 4.42 +/- 2.72 mm, respectively). The relationship between the jaws and thereby the esthetic profile could be improved in all cases. All patients were satisfied with the dental rehabilitation and the achieved new esthetic appearance. CONCLUSIONS: HLFO combined with iliac bone grafting is a feasible preprosthetic technique prior to implant insertion in cases of severe atrophy of the maxillary alveolar ridge, leading to satisfying implant survival and rehabilitation of function.


Subject(s)
Alveolar Ridge Augmentation/methods , Maxilla/surgery , Osteotomy, Le Fort/classification , Adult , Aged , Alveolar Bone Loss/classification , Alveolar Process/pathology , Atrophy , Bone Transplantation , Cuspid , Dental Implants , Dental Restoration Failure , Esthetics, Dental , Feasibility Studies , Female , Follow-Up Studies , Humans , Longitudinal Studies , Male , Mastication/physiology , Maxilla/pathology , Middle Aged , Molar , Osseointegration/physiology , Patient Satisfaction , Treatment Outcome
17.
Article in English | MEDLINE | ID: mdl-12324781

ABSTRACT

OBJECTIVE: Little data exist regarding the use of biodegradable plates and screws for the internal fixation of human mandibular fractures. The purpose of this study was to evaluate the stability of biodegradable, self-reinforced poly-L-lactide plates and screws for the internal fixation of fractures of the human mandible. STUDY DESIGN: Twenty-two individuals (14 male, 8 female; average age, 26.3 years) with a variety of fracture patterns of the mandible underwent management with a biodegradable fixation system. After surgery, maxillomandibular fixation was applied in 3 cases. Images (panoramic radiograph, computed tomographic scan) were taken immediately after surgery and at the 4-week, 8-week, 12-week, and 24-week intervals. The follow-up period averaged 49.1 weeks (range, 22 to 78 weeks). RESULTS: Mucosal dehiscences over the resorbable devices were present in 2 patients. In 1 of these 2 cases, the material had to be replaced with titanium plates. Mucosal healing and consolidation of the fracture were normal in all other patients. CONCLUSION: Self-reinforced biodegradable osteosynthesis materials provide a reliable and sufficient alternative to conventional titanium plate systems.


Subject(s)
Absorbable Implants , Fracture Fixation, Internal/instrumentation , Jaw Fixation Techniques/instrumentation , Mandibular Fractures/surgery , Adolescent , Adult , Aged , Biocompatible Materials , Bone Plates , Bone Screws , Child , Female , Follow-Up Studies , Humans , Male , Middle Aged , Polyesters
18.
Clin Oral Implants Res ; 13(6): 610-6, 2002 Dec.
Article in English | MEDLINE | ID: mdl-12519335

ABSTRACT

We developed a head-mounted display (HMD) with integrated computer-generated stereoscopic projection of target structures and integrated it into visit, a specific oral implant planning and navigation software. The HMD is equipped with two miniature computer monitors that project computer-generated graphics stereoscopically into the optical path. Its position is tracked by the navigation system's optical tracker and target structures are displayed in their true position over the operation site. In order to test this system's accuracy and spatial perception of the viewer, five interforaminal implants in three dry human mandibles were planned with visit and executed using the stereoscopic projection through the HMD. The deviation between planned and achieved position of the implants was measured on corresponding computed tomography (CT) scan images recorded post-operatively. The deviation between planned and achieved implant position at the jaw crest was 0.57 +/- 0.49 mm measured from the lingual, and 0.58 +/- 0.4 mm measured from the buccal cortex. At the tip of the implants the deviation was 0.77 +/- 0.63 mm at the lingual and 0.79 +/- 0.71 mm at the buccal cortex. The mean angular deviation between planned and executed implant position was 3.55 +/- 2.07 degrees. The present in vitro experiment indicates that the concept of preoperative planning and transfer to the operative field by an HMD allows us to achieve an average precision within 1 mm (range up to 3 mm) of the implant position and within 3 degrees deviation for the implant inclination (range up to 10 degrees ). Control during the drilling procedure is significantly improved by stereoscopic vision through the HMD resulting in a more accurate inclination of the implants.


Subject(s)
Data Display , Dental Implantation, Endosseous/instrumentation , Surgery, Computer-Assisted/instrumentation , Computer Graphics , Dental Implants , Equipment Design , Humans , Image Interpretation, Computer-Assisted/instrumentation , Mandible/surgery , Osteotomy/methods , Patient Care Planning , Surface Properties
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