Personalised graded psychological intervention on negative emotion and quality of life in patients with breast cancer.

BACKGROUND: Psychological factors are a risk factor for the incidence of breast cancer and have a significant impact on patient prognosis. OBJECTIVE: The present study aims to investigate the effects of personalised graded psychological intervention on negative emotion and quality of life in patients with breast cancer. METHODS: A total of 200 patients with breast cancer were randomly divided into two groups: an experimental group (n= 100) and control group (n= 100). Both groups received routine nursing care. The experimental group received personalised graded psychological intervention care, and the control group received routine nursing measures. After 2 months of standard treatment, the patients' quality of life and negative emotions were evaluated using the self-rating depression scale (SDS), self-rating anxiety scale (SAS), social support rating scale (SSRS) and quality of life measurement scale (FACT-B) scoring criteria. RESULTS: There were no significant differences in the general data between the two groups (p> 0.05). Furthermore, there were no significant differences in the SDS, SAS, SSRS and FACT-B scores between the two groups before personalised graded psychological intervention (p> 0.05). After the intervention, the experimental group exhibited an improved nursing effect compared with the control group. The SDS and SAS scores were lower in the experimental group than in the control group (p< 0.05); after the intervention, the SDS and SAS scores were significantly lower in the experimental group than in the control group (p< 0.05). The SSRS and FACT-B scores were higher in the experimental group than in the control group (p< 0.05), and the experimental group's post-intervention SSRS and FACT-B scores were significantly higher than before the intervention (p< 0.05). CONCLUSIONS: The use of personalised graded psychological intervention for the nursing of patients with breast cancer in clinical practice can significantly reduce patients' negative emotions as well as improve positive emotions and quality of life; thus, this method can be popularised in the nursing process.

LncRNA MAGI2-AS3-Encoded Polypeptide Restrains the Proliferation and Migration of Breast Cancer Cells.

Accumulating articles have reported the coding potential of long non-coding RNAs (lncRNAs). However, only a few lncRNAs-encoded peptides have been studied. Breast cancer (BRCA) progression-related gene modules were determined by weighted gene co-expression network analysis (WGCNA). Cell viability, proliferation, and migration capacities were assessed by Cell counting kit-8 (CCK8), 5-ethynyl-2'-deoxyuridine (EdU), and transwell assays. Immunofluorescence (IF) assay was implemented to observe protein expression. Co-immunoprecipitation (Co-IP) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) were employed to analyze MAGI2 antisense RNA 3 (MAGI2-AS3)-ORF5-interacted proteins. WGCNA identified that MEpurple and MEblack modules were significantly negatively correlated with T stage in BRCA patients. MAGI2-AS3 was screened as one of the differentially expressed (DE) lncRNAs with translational potential in MEblack and MEpurple modules in BRCA. The data in The Cancer Genome Atlas (TCGA) uncovered that MAGI2-AS3 abundance was significantly decreased in invasive BRCA patients, and it had high diagnostic and prognostic values. MAGI2-AS3-ORF5 notably restrained BRCA cell viability, proliferation, and migration. Mechanically, MAGI2-AS3-ORF5 might affect the progression of BRCA cells by binding to extracellular matrix (ECM)-related proteins. MAGI2-AS3-ORF5 played an anti-tumor role by inhibiting BRCA cell viability, proliferation, and migration. MAGI2-AS3-ORF5 might modulate BRCA cell migration through ECM-associated proteins.

Application of afatinib combined with np regimen in the treatment of stage iv non-small cell lung cancer and its effect on patient survival.

To explore the application of afatinib combined with NP regimen in the treatment of stage IV non-small cell lung cancer and its effect on patient survival, the data of 100 patients with stage IV non-small cell lung cancer admitted to our hospital from February 2017 to February 2018 were retrospectively analyzed. They were equally divided into observation group and control group, with 50 in each group. The control group was treated with an NP regimen and the observation group was treated with afatinib. The disease control rate (DCR) of the observation group was remarkably higher than that of the control group (P<0.05). The observation group witnessed a markedly higher clinical benefit rate relative to the control group (P<0.05). A remarkably longer median treatment failure time of the observation group was observed as compared to the control group (P<0.001). There was no statistical difference in the incidence of adverse reactions between the observation group and the control group (P>0.05). Afatinib combined with NP regimen treatment increases the clinical benefit rate of patients with stage IV non-small cell lung cancer, improves its short-term efficacy and helps prolong the survival time of patients, with excellent safety profile.

Pretreatment with Methylene Blue Protects Against Acute Seizure and Oxidative Stress in a Kainic Acid-Induced Status Epilepticus Model.

BACKGROUND The aim of the present study was to investigate the potential anticonvulsant effect of methylene blue (MB) in a kainic acid (KA)-induced status epilepticus (SE) model. The effects of MB on levels of oxidative stress and glutamate (Glu) also were explored. MATERIAL AND METHODS Sixty C57BL/6 mice were randomly divided into 5 equal-sized groups: (1) controls; (2) KA; (3) MB 0.5 mg/kg+KA; (4) MB 1 mg/kg+KA; and (5) vehicle+KA. The SE model was established by intra-amygdala microinjection of KA. Behavioral observations and simultaneous electroencephalographic records of the seizures in different groups were analyzed to determine the potential anticonvulsant effect of MB. The influences of MB on oxidative stress markers and glutamate were also detected to explore the possible mechanism. RESULTS MB afforded clear protection against KA-induced acute seizure, as measured by the delayed latency of onset of generalized seizures and SE, decreased percentage of SE, and increased survival rate in mice with acute epilepsy. MB markedly increased the latency to first onset of epileptiform activity and decreased the average duration of epileptiform events, as well as the percentage of time during which the epileptiform activity occurred. Administration of MB prevented KA-induced deterioration of oxidative stress markers and Glu. CONCLUSIONS MB is protective against acute seizure in SE. This beneficial effect may be at least partially related to its potent antioxidant ability and influence on Glu level.

Effect of mechanochemical activation of natural phosphorite structure as well as phosphorus solubility.

Mechanochemical treatment of phosphate rock is considered as an effective and ecologically clean way of treating the medium- and low-grade phosphorite which could be used as fertilizer instead of the high-grade phosphorite. In order to investigate the effects of different milling times on the mechanochemically activated phosphorite (lower total phosphorus content) by more efficient milling equipment with enhanced milling speed, phosphorus solubility in citric acid and structural characteristics of natural and mechanochemically activated phosphorite from Yichang, China were studied using scanning electron microscope, infrared spectroscopy and X-ray diffraction. Phosphorus solubility in citric acid increased proportionately with the milling time until 30 min (57.51%), but then gradually reached an equilibrium with the maximum (59.03%) in 50 min. These changes were mainly manifested in considerably reduced particle size, decreased crystallinity and increased structural defects of phosphorite due to substitution of PO43- with CO32- and the incorporation of OH-. With the incorporation of CO32- and OH-, the non-activated carbonate-fluorapatite (type B) was transformed into a mixture of carbonate-fluorapatite, hydroxyapatite, fluorocarbon hydroxyapatite and/or carbonate apatite, respectively during the process of mechanochemical activation. As a result of the structural and phase transformations after mechanochemical activation, phosphorus solubility remarkably increased.

Does animal manure application improve soil aggregation? Insights from nine long-term fertilization experiments.

Manure application is widely recognized as a method of improving soil structure and soil fertility due to additional organic matter and nutrient inputs. However, the salinity of animal manure may have a detrimental effect on soil aggregation. The objective of this study was to determine the effects of long-term animal manure application on soil aggregation, binding agents (soil organic carbon, SOC and glomalin-related soil protein, GRSP), and dispersing agents (e.g., Na+) and their relationships based on nine long-term fertilization experiments (12 to 39 yr) across China. The two red soil experiments (Qiyang, QY and Jinxian, JX) and one paddy soil experiment in Jinxian (JX-P) were conducted in southern China (precipitation above 1200 mm yr-1), whereas the other six experiments were established in semi-humid or arid regions in China with precipitation in the range of 500-900 mm yr-1. Each experiment included three treatments as follows: no fertilization (Control), inorganic fertilizer (NP or NPK), and a combination of inorganic fertilizer and animal manure (NPM or NPKM). Long-term animal manure application not only significantly increased the biological binding agents (i.e., SOC and GRSP) in the nine experiments but also considerably increased the dispersing agents (i.e., exchangeable Na+) (P < 0.05), except for the paddy soil experiment. Consequently, soil aggregate stability increased after animal manure application in three experimental sites in southern China but not in the experimental sites in northern China. Aggregate stability had a positive relationship with SOC and GRSP in the experimental sites in southern China (P < 0.01) but a negative relationship with exchangeable Na+ in the experimental sites in northern China (P < 0.05). The Na+ accumulation in soils was negatively related to mean annual precipitation (P < 0.001). Our study demonstrates that the long-term application of animal manure may degrade soil structure via the Na+ accumulation.

Study on the interaction of chromate with bovine serum albumin by spectroscopic method.

The interaction between two chromates [sodium chromate (Na2CrO4) and potassium chromate K2CrO4)] and bovine serum albumin (BSA) in physiological buffer (pH 7.4) was investigated by the fluorescence quenching technique. The results of fluorescence titration revealed that two chromates could strongly quench the intrinsic fluorescence of BSA through a static quenching procedure. The apparent binding constants K and number of binding sites n of chromate with BSA were obtained by the fluorescence quenching method. The thermodynamic parameters enthalpy change (ΔH), entropy change (ΔS) were negative, indicating that the interaction of two chromates with BSA was driven mainly by van der Waals forces and hydrogen bonds. The process of binding was a spontaneous process in which Gibbs free energy change was negative. The distance r between donor (BSA) and acceptor (chromate) was calculated based on Forster's non-radiative energy transfer theory. The results of UV-Vis absorption, synchronous fluorescence, three-dimensional fluorescence and circular dichroism (CD) spectra showed that two chromates induced conformational changes of BSA.

A Greenhouse Assay on the Effect of Applied Urea Amount on the Rhizospheric Soil Bacterial Communities.

The rhizospheric bacteria play key role in plant nutrition and growth promotion. The effects of increased nitrogen inputs on plant rhizospheric soils also have impacted on whole soil microbial communities. In this study, we analyzed the effects of applied nitrogen (urea) on rhizospheric bacterial composition and diversity in a greenhouse assay using the high-throughput sequencing technique. To explore the environmental factors driving the abundance, diversity and composition of soil bacterial communities, the relationship between soil variables and the bacterial communities were also analyzed using the mantel test as well as the redundancy analysis. The results revealed significant bacterial diversity changes at different amounts of applied urea, especially between the control treatment and the N fertilized treatments. Mantel tests showed that the bacterial communities were significantly correlated with the soil nitrate nitrogen, available nitrogen, soil pH, ammonium nitrogen and total organic carbon. The present study deepened the understanding about the rhizospheric soil microbial communities under different amounts of applied urea in greenhouse conditions, and our work revealed the environmental factors affecting the abundance, diversity and composition of rhizospheric bacterial communities.

Study on the effect of magnetic field treatment of newly isolated Paenibacillus sp.

BACKGROUND: Symbiotic nitrogen fixation in plants occurs in roots with the help of some bacteria which help in soil nitrogen fertility management. Isolation of significant environment friendly bacteria for nitrogen fixation is very important to enhance yield in plants. RESULTS: In this study effect of different magnetic field intensity and treatment time was studied on the morphology, physiology and nitrogen fixing capacity of newly isolated Paenibaccilus sp. from brown soil. The bacterium was identified by 16S rDNA sequence having highest similarity (99%) with Paenibacillus sp as revealed by BLAST. Different magnetic intensities such as 100mT, 300mT and 500mT were applied with processing time of 0, 5, 10, 20 and 30 minutes. Of all these treatment 300mT with processing time of 10 minutes was found to be most suitable treatment. Results revealed that magnetic treatment improve the growth rate with shorter generation time leading to increased enzyme activities (catalase, peroxidase and superoxide dismutase) and nitrogen fixing efficiencies. High magnetic field intensity (500mT) caused ruptured cell morphology and decreased enzyme activities which lead to less nitrogen fixation. CONCLUSION: It is concluded that appropriate magnetic field intensity and treatment time play a vital role in the growth of soil bacteria which increases the nitrogen fixing ability which affects the yield of plant. These results were very helpful in future breading programs to enhance the yield of soybean.

[Effects of sewage sludge compost on the growth and photosynthetic characteristics of turfgrass].

The effects of different dosages (0, 0.8%, 2%, 6% and 10%) of sewage sludge compost on the growth and photosynthetic characteristics of three turfgrass, i. e., Festuca arundinacea, Lolium perenne and Poa pratensis were studied. The results showed that the dosage of 6% had the optimal effects. The plant height, leaf area and dry biomass of three turfgrass increased significantly with the increasing sewage sludge compost dosage, while the root/shoot ratio decreased obviously. Compared with the control, the plant height of F. arundinacea, L. perenne and P. pratensis increased by 64.9%-180.8%, 97.3%-200.9% and 39.1%-156.5%, the leaf area per plant increased by 91.3%-417.4%, 186.4%-394.9% and 164.6%-508.3% and the dry biomass per plant increased by 333.4%-867.6%, 138.4%-445.1% and 316.3%-669.2%, respectively. With the increasing dosage of sewage sludge compost, the net photosynthetic rate (Pn), transpiration rate (Tr), intercellular CO2 concentration (Ci) and water use efficiency (WUE) of F. arundinacea increased first and decreased then, the Pn, Tr and WUE of P. pratensis showed a significantly increasing trend, while the Ci decreased after an initial increase, and the Pn and WUE of L. perenne increased gradually, while the Ci and Tr decreased after a gradual increase. With the in- creasing sewage sludge dosage, the contents of chlorophyll a and b of the three turfgrass species increased markedly, and then decreased when the dosage was more than 6%, but the ratio of chlorophyll a/b was little changed.

[Isolation identification and characterization of halotolerant petroleum-degrading bacteria].

To obtain efficient halotolerant petroleum-degrading bacteria, 39 bacteria strains were isolated from 30 petroleum contaminated saline soil samples in Yellow River Delta, an important base of petroleum production in China. One bacterium (strain BM38) was found to efficiently degrade crude oil in highly saline environments based on a series of liquid and soil incubation experiments. According to its morphology, physiochemical characteristics and 16S rDNA sequence analysis, this strain was identified as Pseudomonas putida. Moreover, a series of liquid incubation experiments were conducted to investigate its characteristics such as halotolerance, biosurfactants production and degrading efficiency for various hydrocarbons. The salt resistance test demonstrated that strain BM38 grew well at NaCl concentrations ranging from 0.5% to 6.0%. Petroleum degradation experiments showed that strain BM38 could degrade 73.5% crude oil after 7 days in a liquid culture medium containing 1.0% NaCl and remove more than 40% of total petroleum hydrocarbons after 40 days in the soil with 0.22% and 0.61% of salinity, these results proved that the strain was effective in removing petroleum hydrocarbons. Strain BM38 could produce a bioemulsifier in a liquid culture medium. The NaCl concentration had the significant effect on the EI24 of fermentation broth, which decreased sharply if the NaCl concentration was greater than 1.0%. However, the EI24 of BM38 was still quite high in the presence of 2.0% of NaCl, and the value was 61.0%. Furthermore, this strain was also able to grow in mineral liquid media amended with hexadecane, toluene, phenanthrene, isooctane and cyclohexane as the sole carbon sources. Among these hydracarbons, strain BM38 showed relatively high ability in degrading n-alkanes and aromatic hydracarbons. The results indicated that strain BM38 had potential for application in bioremediation of petroleum-contaminated saline soil.