Molecularly imprinted electrochemical biosensor for thrombin detection by comparing different monomers.

Aim: Investigating molecularly imprinted polymers (MIPs) in electrochemical biosensors for thrombin detection, an essential protein biomarker. Comparing different monomers to showcase distinct sensitivity, specificity and stability advantages. Materials & methods: Dopamine, thionine and ethanolamine serve as monomers for MIP synthesis. Electrochemical methods and atomic force microscopy characterize sensor surfaces. Performance is evaluated, emphasizing monomer-specific electrochemical responses. Results: Monomer-specific electrochemical responses highlight dopamine's superior signal change and stability over 30 days. Notably, a low 5 pg/ml limit of detection, a broad linear range (5-200 pg/ml) and enhanced selectivity against interferents are observed. Conclusion: Dopamine-based MIPs show promise for high-performance electrochemical thrombin biosensors, suggesting significant applications in clinical diagnostics.

Development of aptamer-based ELISA method for d-dimer detection.

This study developed an aptamer-based enzyme-linked immunosorbent assay (ELISA) system for sensitive detection of the d -dimer molecule, which is significantly elevated in several severe diseases. Aptamers are known to have advantages, such as longer stability and usability at different temperatures and in different complex samples, over antibodies, which are generally used in traditional ELISA methods. In the present work, the aptamer-based ELISA system was capable of quantitatively determining in a wide range (100 ng/ml─10 µg/ml) and remain stable for a couple of months even under ordinary room conditions. Validation of the developed system was evaluated by spiked human serum samples with high accuracy. With more comprehensive project steps, the developed aptamer-based ELISA system could be transformed into a platform that offers a sensitive, portable, and easy-to-use tool for analyzing d -dimer molecules.