ABSTRACT
It is known that cell extracts of various algae have antifungal activity against microorganisms in vitro. Antifungal activities of Ulva lactuca, Chlorella vulgaris, Chlorella minutissima, and Chlorella protothecoides were investigated against: Aspergillus niger, Alternaria alternata, and Penicillium expansum fungi to present their fungicide potentials. Aspergillus niger, Alternaria sp., and Penicillium expansum are typical soft-rotting fungi and cause important loss of apple fruit in the storage. In vitro antifungal activity was evaluated by agar disc diffusion assay against pathogenic apple rot fungi. As a result, almost all of the extracts obtained from algae species were revealed to have antifungal activity against selected fungal pathogens. Free radical-scavenging activity of the extracts was determined with 1,1-diphenyl-2 picryl hydrazyl (DPPH) free radical-scavenging method. Extract of C. protothecoides was determined to have a stronger antioxidant activity than other algae extracts. This study reveals that the potential of algae should be investigated for the production of natural fungicide for pharmaceutical and food industries.
Subject(s)
Chlorella vulgaris/chemistry , Fungicides, Industrial/pharmacology , Malus/microbiology , Plant Extracts/pharmacology , Ulva/chemistry , Alternaria/drug effects , Alternaria/growth & development , Antifungal Agents/pharmacology , Aspergillus/drug effects , Aspergillus/growth & development , Fungicides, Industrial/chemistry , Microbial Sensitivity Tests , Penicillium/drug effects , Penicillium/growth & development , Plant Diseases/microbiology , Plant Extracts/chemistryABSTRACT
In this study, silver-stearate nanoparticles (AgStNPs) have been produced from silver nitrate solutions by replacing the nitrate by stearate ions and then reducing by thermal treatment. AgStNPs were characterized by particle size, zeta-potential, ATR-FTIR, contact angle and SEM (scanning electron microscopy) analyses. The mean particle size and zeta potential of AgStNPs were determined to be 69.22±7.30nm and +8.02±0.88mV. ATR-FTIR analysis showed characteristic IR bands of stearate, revealing the two strong peaks at 2848 and 2915cm-1 associated to symmetric (νs(CH2)) and asymmetric (νas(CH2)) stretching vibrations of methylene groups, respectively. On the other hand, the scissoring and rocking modes of methylene group were observed at 1470 and 718cm-1, respectively. Nanomorphological characterization by SEM revealed a layered morphology of AgStNPs. Contact angle measurements demonstrated that a contact angle of water drop on glass coated with AgStNPs was found to be 108.76°, which proved the strong hydrophobic properties of AgStNPs. AgStNPs seemed to be very effective in inhibiting foodborne pathogens (Salmonella Typhimurium, Escherichia coli O157:H7, Staphylococcus aureus, Listeria monocytogenes and Candida albicans). The results suggest the use of AgStNPs as a coating material to reduce biofilm or biofouling formation in terms of achieving appropriate food contact surfaces and higher hygiene/easier sanitation due to their strong hydrophobic and antimicrobial properties.
