The changes of antioxidant system and intestinal bacteria in earthworms (Metaphire guillelmi) on the enhanced degradation of tetracycline.

Tetracycline (TC) in soil severely imperils food security and ecosystem function. Metaphire guillelmi is a common species in farmland. It could impact the degradation of antibiotics. However, how it affects is rarely unknown. Hence, the present study aimed to investigate the effects of M. guillelmi on the TC degradation in soil and the changes of the antioxidant system and intestinal bacteria in M. guillelmi. The treatments that M. guillelmi was inoculated on soil contaminated with different TC concentrations were contrasted with those without M. guillelmi. After 21 days, the degradation rate of TC significantly increased by 13.70%, 18.14% and 29.01% at 10, 50 and 100 mg kg -1 TC dose, respectively, due to the inoculation of M. guillelmi. The half-life of TC was also shortened nearly by 1/3 to 2/3. Superoxide dismutase (SOD) increased in a dose-dependent manner with the increase of TC concentration on the 7th and 14th day. Catalase (CAT) and glutathione S-transferase (GST) presented an inverted U-shaped dose response on the 7th day, and the peak of enzyme activities occurred at TC concentration of 0.1, 1 mg kg -1 (CAT) and 0.1 mg kg -1 (GST). Malondialdehyde (MDA) contents did not change significantly. At the phylum level, only Verrucomicrobia significantly decreased under 1 mg kg -1 and 100 mg kg -1 TC dose. Genus Paracoccus, Singulisphaera, Acinetobacter and Bacillus significantly increased and became the dominant bacterium during the TC degradation. Overall, the antioxidant system and intestinal bacteria of M. guillelmi were affected by the different concentrations of TC pollution, which provided new ideas for the research of mechanism of TC degradation by earthworms in the future.

Effects of Salinity on Earthworms and the Product During Vermicomposting of Kitchen Wastes.

Population growth and social changes have recently contributed to an exaggerated increase in kitchen wastes in China. Vermicomposting has recently been recognized as an effective and eco-friendly method of organic waste treatment through the combination of earthworms and microbes. However, the influence of salt in kitchen wastes on vermicomposting have been unknown. The goal of this study was to analyze the influence of different salinities on earthworms (Eisenia fetida) and the products during the vermicomposting of kitchen wastes. In our research, kitchen wastes were divided into four different salinities: 0% (A), 0.1% (B), 0.2% (C) and 0.3% (D). The chemical characters of substrates and earthworm growth were measured on the 14th day and the 28th day of composting. Our results show that the high salinity (measured >0.2%) prevented earthworms from properly growing and had negative effects on quality of products in composting. T2 (measured salinity = 0.2%) had the highest average body weight, nitrate nitrogen, and available phosphorus. Thus, the salinity of kitchen wastes should be pretreated to less than 0.2% before vermicomposting.

Effects of different lipid contents on growth of earthworms and the products during vermicomposting.

In China, the production of kitchen waste was more than 97 million tons per year in 2016. The high lipid content of kitchen waste makes it hard to degrade. Vermicomposting is a kind of low-cost biotechnology through the combination of earthworms and microbes, which could be considered in converting kitchen waste to valuable products. However, the effect of the lipid in kitchen waste on earthworms and composting is not known yet. The objective of this study was to analyze the effects of lipid on vermicomposting and to derive the most suitable lipid content in vermicomposting. In our study, kitchen waste with four different lipid contents was prepared: 0% (A), 5% (B), 10% (C) and 15% (D). The earthworm growth and chemical characters of substrates were measured at the interim (14 days) and the end (28 days) of vermicomposting. Our results showed that the high content of lipid had negative effects on growth of earthworms and products in vermicomposting. The kitchen waste with lower lipid content could be better composted by earthworms. The average body weight of the earthworm increased by 9% in treatment A. Total nitrogen, available phosphorus, available potassium and nitrate nitrogen of treatment A with 0% (measured value: 5%) lipid content were significantly higher than those in other treatments. Meanwhile, treatment A had the lowest carbon/nitrogen ratio which might be due to hindrances in the breathing and activities of earthworms. The high content of lipid makes growth of aerobic bacteria such as nitrifying bacteria difficult.