1.
Genet Mol Res
; 15(3)2016 Sep 23.
Article
in English
| MEDLINE
| ID: mdl-27706785
ABSTRACT
Fusarium oxysporum strain BM-201 was treated with ultraviolet (UV) radiation to obtain a high pectinase-producing strain. Mutant UV-10-41 was obtained and then treated by diethyl sulfate. Next, the mutant UV-diethyl sulfate-43 derived from UV-10-41 was selected as high pectinase-producing strain. Mutant UV-diethyl sulfate-43 was incubated on slant for 10 generations, demonstrating that the pectinase-producing genes were stable. Pectinase activity reached 391.2 U/mL, which is 73.6% higher than that of the original strain.
Subject(s)
Fusarium/enzymology , Fusarium/genetics , Mutagenesis/genetics , Polygalacturonase/biosynthesis , Sulfuric Acid Esters/toxicity , Ultraviolet Rays , Dose-Response Relationship, Radiation , Fermentation/drug effects , Fermentation/radiation effects , Fusarium/isolation & purification , Fusarium/radiation effects , Hexuronic Acids/metabolism , Mutagenesis/drug effects , Mutagenesis/radiation effects , Reference Standards , Time Factors
2.
Yao Xue Xue Bao
; 22(12): 896-900, 1987 Dec.
Article
in Chinese
| MEDLINE
| ID: mdl-2967026
