TNF-α increases bone marrow mesenchymal stem cell migration to ischemic tissues.

The objective of this study was to analyze the influence of TNF-α on rat mesenchymal stem cells (MSCs) and to assess feasibility of MSC transplantation to repair ischemic injury. In this study, adhesion molecules and cell specific surface markers on MSCs were measured after exposure to different concentrations of TNF-α. MSCs stimulated with varying concentrations of TNF-α were cultured with aortic endothelial cells, and the adhesion rate was measured. MSCs were then stimulated with an optimum concentration of TNF-α as determined in vitro, and injected intravenously into rats with ischemic hind limb injury. The number of MSCs in muscle samples from the ischemic area was counted. The results showed that (1) TNF-α induced a concentration-dependent increase in VCAM-1 expression in MSCs, whereas the expression of L-selectin, ICAM-1 and VLA-4 did not change significantly. Expression of MSC-specific antigens was unchanged. (2) MSCs pretreated with 10 ng/ml TNF-α showed significantly increased adhesion to endothelial cells in vitro, and accumulated to a greater extent in the areas of ischemic damage in rat hind limbs. We were able to conclude that TNF-α has no effect on expression of MSC-specific markers, but can increase the expression of VCAM-1 on rat MSCs. Suitable concentrations of TNF-α can promote MSC adhesion to endothelial cells and migration to damaged tissue.

Sectional anatomy of the fetal brain in uterus at term on the sagittal plane.

OBJECTIVE: To provide sectional anatomic data for the precise localization of developmental malformation of fetal brain in sagittal magnetic resonance imaging (MRI). METHOD: After abdominal and pelvic MRI scanning, the gravid specimen was cut into serial sagittal slices in correspondence with MRI in a low temperature laboratory to demonstrate the structures of fetal brain. RESULT: (1) Directional determination of the sloping and rotating fetal head. From the serial sagittal sections of pregnant cadaver at term, we concluded that, the longitudinal lying and cephalic presentation fetal had run into maternal pelvis, and rotated and sloped to right. Anteroposterior position and median sagittal plane of the fetal was in correspondence with his mother's. (2) Seven serial sagittal sections of the fetal brain were obtained through lateral surface of the right cerebral hemisphere, lateral sulcus, internal capsule, median sagittal plane, middle cerebellar peduncle, brainstem, and lateral surface of the left cerebral hemisphere. CONCLUSION: Through the comparison study between sagittal sections and corresponding MRI of fetal brain at term, we could obtain morphological anatomic structures and MRI of fetal brain, providing morphological demonstration of the intrauterine development of fetal brain and auxiliary diagnosis of ultrasound and MRI in pregnant woman.

Sectional anatomy of the peritoneal reflections of the upper abdomen in the coronal plane.

PURPOSE: The purpose of this study was to provide practical anatomic data for the imaging diagnosis and surgical treatment of the diseases of the subphrenic spaces. METHODS: The sectional anatomy of the subphrenic spaces on the coronal plane was investigated on serial coronal sections of the upper abdomen of 30 Chinese adult cadavers. RESULTS: The space between the anterior margin of gastropancreatic fold and the posterior layer of hepatogastric ligament is the only direct pathway between the superior and inferior recesses of the lesser sac. That pathway can be divided into 3 types on the coronal plane. The right layer of the gastrophrenic ligament is continuous with the posterior layer of the lesser omentum, and its left layer is continuous with the right layer of the phrenosplenic ligament and the posterior layer of the gastrosplenic ligament. The gastropancreatic fold is continued to the left and right layers of the gastrophrenic ligament upwards. The bare area of the stomach is located between the left and right layers of the gastrophrenic ligament; its existing rate is 100%. The bare area of the spleen is located among the phrenosplenic ligament, gastrosplenic ligament, splenorenal ligament, and splenocolic ligament. Its greatest width exists between the two layers of the splenorenal ligament. It can be divided into the splenic hilus and splenorenal parts. CONCLUSION: The coronal section is dominant to show the anatomic relationships of the gastrophrenic ligaments and the gastropancreatic folds, and the bare area of the stomach.