Thirteen undescribed diterpenoid quinones derived from the rhizomes of Salvia miltiorrhiza and their anti-tumor activities.

Thirteen undescribed diterpenoid quinones were isolated from the dried roots of Salvia miltiorrhiza. Their structures were determined by extensive analysis, including NMR, HRESIMS, and IR. Their absolute configurations were determined by X-ray diffraction, calculated and experimental circular dichroism spectroscopy, and optical rotation. In the evaluation of bioactivities, salviadionether obviously inhibited the proliferation of HCT-116 cells. R-(+)-salmiltiorin E and R-(+)-grandifolia D both showed inhibitory activities on a variety of tumor cells. Salvianone ester A showed strong cytotoxicity to tumor-repopulating cells (TRCs) with an IC50 value of 2.19 µM.

Three 11,12-seco-tanshinone derivatives from the rhizomes of Salvia miltiorrhiza.

Three new compounds named tanshinoic acid A (1), tanshinoic acid B (2), and tanshinoic acid C (3) were isolated from the rhizomes of Salvia miltiorrhiza, together with two known compounds methyl salvianolate A (4) and methyl salvianolate C (5). Their structures were determined on the basis of spectroscopic data (UV, IR, HRESIMS, 1 D and 2 D NMR). The in vitro assay indicated that 4 and 5 could improve the survived rate of cells on oxygen glucose deprivation (OGD) induced neurons damage model and glutamate-induced neurons damage model at a concentration of 10 µM. [Formula: see text].

New diterpenoid quinones derived from Salvia miltiorrhiza and their cytotoxic and neuroprotective activities.

One new tanshinone derivative, which possesses an unusual 6/6/5/6 fused-ring skeleton system (1), together with four new five-membered lactone benzohexa-membered ring compounds (2, 3, 4A and 4B), and three new carboxyl substituted 5,5-spiroketal compounds (5-7), were isolated from the dried rhizomes of Salvia miltiorrhiza. The structures of these compounds were determined by multiple spectral analyses (UV, IR, NMR, and HR-ESI-MS). In addition, the absolute configurations were established by X-ray diffraction experiments, calculated and experimental circular dichroism spectra. Evaluation of antitumor activity showed that 1 had strong cytotoxicity to tumor-repopulating cells (TRCs) with an IC50 value of 2.83 µM. In the evaluation of neuroprotective activity, 4A and 6 showed a strong improvement in the survival rates of SK-N-SH cell injury induced by oxygen glucose deprivation (OGD).

Two new tanshinone derivatives from the rhizomes of Salvia miltiorrhiza and their antiviral activities.

Two new naturally occurring products named salviamine G (1) and 4-methyl-9-(ethoxycarbonyl)-8-naphthoic acid (2) were isolated from the rhizomes of Salvia miltiorrhiza. Their structures were elucidated using spectroscopic data (UV, IR, HRESIMS, 1D and 2D NMR). Compounds 1 and 2 were screened for their inhibitory activity against HSV-1 and influenza A (H3N2) using acyclovir (ACV, IC50 = 0.67 µM) and oseltamivir (IC50 = 2.01 µM) as a positive control. Compound 1 exhibited moderate inhibitory activity against HSV-1 and influenza A (H3N2) with IC50 values of 11.11 and 8.62 µM, respectively.