ABSTRACT
Objective To analyze the general characteristics of voluntary blood donors, and to provide the basis for establishing effective recruitment mechanism. Methods The information of all whole blood donors (including mobile blood collection cars on the streets , blood donation houses and team blood donors) from 2006 to 2015 was obtained from the Zhejiang blood management information system. The sex, age, occupation, weight, and the amount of blood donation at a time of blood donors were descriptively analyzed. Results There were 5299700 frequencies of donating blood in Zhejiang Province from 2006 to 2015. The annual frequency was on the rise, with an average annual growth rate of 2.99％. There were 3098500 frequencies of male and 2201300 of female with the ratio of 1.4 :1. The frequency of female has declined since 2010. The majority of voluntary blood donors ranged from 18 to 45 years old, and the percentage was 91.44％ . The ratios of staff, students and workers were 14.61％, 12.27％, 10.09％, respectively. The annual frequency of staff was on the rise, with an average annual growth rate of 7.71％. The frequency of workers was on the decline, and the frequency of students fluctuated over the years. The ratio of blood donation 400 mL at a time was 41.23％. For 400 mL blood donation at a time, the ratio of male donors (48.86％) was significantly higher than the female donors (30.50％, P<0.001) . In addition, the ratio of blood donation 400 mL at a time was proportional to age and body weight (P<0.001) . Conclusion In Zhejiang Province, the frequency of blood donation has increased year by year. Staff, students and workers with 18 to 45 years old are the main force of blood donation. The frequency of female blood donation has a tendency of dropping, and the ratio of blood donation 400 mL at a time is relatively low. It's necessary to strengthen the propaganda of blood donation.
ABSTRACT
To evaluate the effects of recombinant porcine interleukin-18 (rpIL-18) on the replication of viruses in host cells and proliferation of lymphocytes, porcine IL-18 (pIL-18) isolated from a domestic big-white porcine breed found in the Henan province (HN) was cloned using a reverse transcriptase-PCR. The cloned HN pIL-18 contained an ORF of 579 base pairs encoding a 192-amino-acid precursor protein. The amino acid sequence of HN pIL-18 was compared with all the other pIL-18 amino acid sequences and varied by at least one amino acid to the consensus of all the others available. HN pIL-18 mature protein gene was inserted into a prokaryotic vector pGEX-4T-1 and expressed in Escherichia coli BL21. The expression of glutathione-S-transferase-pIL18 fusion protein was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis. The rpIL-18 induced in vitro proliferation of concanavalin-A-stimulated porcine splenocytes, as revealed by the MTT assay. We studied the antiviral activities of the rpIL-18 on the replication of porcine reproductive and respiratory syndrome virus (PRRSV), pseudorabies virus (PRV), and porcine parvovirus (PPV) cultured in two homologous cell lines. The results suggested that rpIL-18 can stimulate the proliferation of lymphocytes and inhibit viral pathogens infecting the porcine population.