ABSTRACT
Content available: Author Audio Recording.
Subject(s)
End Stage Liver Disease/complications , Esophageal and Gastric Varices/surgery , Hepatic Encephalopathy/complications , Portasystemic Shunt, Transjugular Intrahepatic , Pregnancy Complications/surgery , Adult , Cesarean Section , Decompression, Surgical/methods , Esophageal and Gastric Varices/complications , Female , Humans , Pregnancy , Pregnancy Trimester, ThirdABSTRACT
BACKGROUND: The detection of viral respiratory tract infections has evolved greatly with the development of PCR based commercial systems capable of simultaneously detecting a wide variety of pathogens. OBJECTIVES: Evaluate the relative performance of two commercial broad range systems for the detection of viral agents in clinical respiratory tract specimens from immunocompromised children. STUDY DESIGN: A total of 176 patient samples were included in the analysis, representing only the first sample collected for each patient, and excluding failed reactions. Samples were de-identified and assayed in parallel using two different, broadly multiplexed PCR systems: ResPlex™ II Panel v2.0 (ResPlex), Qiagen, Hilden, Germany and FilmArray(®) Respiratory Panel (FilmArray), Idaho Technology Inc., Salt Lake City, UT. Method comparison was based upon pair-wise concordance of results according to patient age, viral target and number of targets detected. RESULTS: The two systems showed an overall concordance, by patient, of 83.8% (p=0.0001). FilmArray detected at least one target in 68.8% of samples, while ResPlex detected at least one target in 56.8%. ResPlex failed to detect 20.7% of FilmArray positives, and FilmArray failed to detect 4% of ResPlex positives. The relative performance of each system (including which system detected a higher number of positive samples) varied when stratified by target viral pathogen. CONCLUSIONS: Broadly multiplexed PCR is an effective means of detecting large numbers of clinically relevant respiratory viral pathogens.
Subject(s)
Immunocompromised Host , Multiplex Polymerase Chain Reaction/methods , Respiratory System/virology , Respiratory Tract Infections/diagnosis , Virus Diseases/diagnosis , Viruses/isolation & purification , Adolescent , Child , Child, Preschool , Humans , Infant , Molecular Diagnostic Techniques/methods , Reagent Kits, Diagnostic , Reproducibility of Results , Respiratory Tract Infections/virology , Sensitivity and Specificity , Virus Diseases/virology , Viruses/classification , Viruses/geneticsABSTRACT
Probe-based detection of mecA, lukS/F-PV (Panton-Valentine leukocidin), and tst virulence genes in 435 isolates of Staphylococcus aureus had comparable sensitivity and specificity to end-point polymerase chain reaction as a reference standard.
