ABSTRACT
Intercropping technology and stabilizing materials are common remediation techniques for soils contaminated with heavy metals. This study investigated the feasibility of the Gynostemma pentaphyllum (G. pentaphyllum)/Helianthus annuus L. (H. annuus) intercropping system on arsenic (As) contaminated farmland through field and pot experiments and the regulation of plant As absorption by the application of mixed stabilizing materials in this intercropping system. Field experiments demonstrated that intercropping with H. annuus increased the As concentration in G. pentaphyllum leaves to 1.79 mg kg-1 but still met the requirements of the national food standard of China (2 mg kg-1) (GB2762-2017). Meanwhile, G. pentaphyllum yield in the intercropping system decreased by 15.09%, but the difference was insignificant (P > 0.05). Additionally, the As bioconcentration (BCA) per H. annuus plant in the intercropping system was significantly higher than that in the monoculture system, increasing by 76.37% (P < 0.05). The pot experiment demonstrated that when granite powder, iron sulfate mineral, and "Weidikang" soil conditioner were applied to the soil collectively, G. pentaphyllum leaf As concentration in the intercropping system could be significantly reduced by 42.17%. Rhizosphere pH is the most crucial factor affecting As absorption by G. pentaphyllum in intercropping systems. When these three stabilizing materials were applied simultaneously, the As bioaccumulation (BCA) per H. annuus plant was significantly higher than that of normal intercropping treatment, which increased by 71.12% (P < 0.05), indicating that the application of these stabilizing materials significantly improved the As removal efficiency of the intercropping system. Dissolved organic carbon (DOC) concentration in the rhizosphere soil is the most pivotal factor affecting As absorption by H. annuus. In summary, the G. pentaphyllum-H. annuus intercropping model is worthy of being promoted in moderately As polluted farmland. The application of granite powder, iron sulfate mineral, and "Weidikang" soil conditioner collectively to the soil can effectively enhance the potential of this intercropping model to achieve "production while repairing" in the As polluted farmland.
Subject(s)
Arsenic , Helianthus , Soil Pollutants , Arsenic/analysis , Gynostemma , Feasibility Studies , Powders , Biodegradation, Environmental , Silicon Dioxide , Soil , Iron , Sulfates , Soil Pollutants/analysis , CadmiumABSTRACT
This work aimed to test the hypothesis that rainwater-borne hydrogen peroxide (H2O2) can affect arsenic uptake by rice plants and emission of greenhouse gases in paddy rice systems. A mesocosm rice plant growth experiment, in conjunction with rainwater monitoring, was conducted to examine the effects of rainwater input on functional groups of soil microorganisms related to transformation of arsenic, carbon and nitrogen as well as various arsenic species in the soil and plant systems. The fluxes of methane (CH4), nitrous oxide (N2O) and carbon dioxide (CO2) were measured during selected rainfall events. The results showed that rainwater-borne H2O2 effectively reacted with Fe2+ present in paddy soil to trigger a Fenton-like reaction to produce â¢OH. Both H2O2 and â¢OH inhibited As(V)-reducing microbes but promoted As(III)-oxidizing microbes, leading to a net increase in arsenate-As that is less phytoavailable compared to arsenite-As. This impeded uptake of soil-borne As by the rice plant roots, and consequently reduced the accumulation of As in the rice grains. The input of H2O2 into the soil caused more inhibition to methanogens than to methane-oxidizing microbes, resulting in a reduction in CH4 flux. The microbes mediating the transformation of inorganic nitrogen were also under oxidative stresses upon exposure to the rainwater-derived H2O2. And the limited conversion of NO3- to NO played a crucial role in reducing N2O emission from the paddy soils. The results also indicated that the rainwater-borne H2O2 could significantly affect other biogeochemical processes that shape the wider ecosystems, which is worth further investigations.
Subject(s)
Arsenic , Greenhouse Gases , Oryza , Greenhouse Gases/analysis , Ecosystem , Hydrogen Peroxide , Soil/chemistry , Nitrogen , Methane/analysis , Nitrous Oxide/analysis , Agriculture/methodsABSTRACT
Selecting suitable agronomic measures can strengthen the application of intercropping in the remediation of cadmium (Cd)-contaminated soil. In this study, the effects of different planting densities and fertilizer applications on the crop growth and Cd absorption of a pumpkin (Cucurbita moschata)-Amaranthus hypochondriacus L. intercropping system was determined. The goal was to provide enhanced means and a scientific basis for the promotion and application of this intercropping system. The Cd content of pumpkin in different planting systems was lower than the national food safety standard (0.05 mg kg-1). In the IN-1 (4 pumpkin plants intercropped with 200 A. hypochondriacus plants) and IN-2 (4 pumpkin plants intercropped with 400 A. hypochondriacus plants) intercropping systems, the bioconcentration amount (BCA) per plant of Cd in A. hypochondriacus increased by 32.43% and 25.25%, respectively, compared with that of the monocropping system (P < 0.05). The IN-2 system had the highest equivalent ratio of heavy metal removal (3.08), indicating that this model had a substantial advantage for removing Cd. The land equivalent ratio of IN-1 (2.89) and IN-2 (2.60) was significantly higher than that of other intercropping systems, indicating that these two models had obvious yield advantages. In our study, chicken manure was the best at promoting the growth and yield of the two plants and sludge treatment significantly enhance Cd absorption of A. hypochondriacus. In general, intercropping four pumpkin plants with 400 A. hypochondriacus plants and applying chicken manure fertilizer can strengthen the application of this intercropping system in Cd-contaminated soil.
Subject(s)
Amaranthus , Cucurbita , Soil Pollutants , Cadmium/analysis , Farms , Fertilizers , Soil , Manure , Soil Pollutants/analysis , Plants , Biodegradation, EnvironmentalABSTRACT
In recent years, research on the safe utilization and green remediation of contaminated soil by intercropping has become common. In this study, the growth of an intercropping system of Luffa cylindrica-Semen cassiae in soil contaminated with medium amounts of arsenic (As) was studied using field (91.60 mg kg-1) and pot (83.34 mg kg-1) experiments. The field experiments showed that intercropping significantly increased the yield per plant of L. cylindrica by 27.36%, while the yield per plant of S. cassiae decreased by 21.66%; however, this difference was not significant. Intercropping reduced the concentration of As in all organs of L. cylindrica but increased the concentration of As in all parts of S. cassiae. The accumulation of As per plant of L. cylindrica was reduced by 20.72%, while that in a single plant of S. cassiae was increased by 201.93%. In addition, the concentration of As in the fruit of these two crops in these two planting modes was low enough to meet the National Food Safety Standard of China (GB2762-2017). In addition, the land equivalent ratio and As metal removal equivalent ratio of the intercropping mode was 1.03 and 2.34, indicating that the intercropping mode had advantages in land use and As removal. In the pot experiment, the biomass and As concentration of L. cylindrica and S. cassiae were roughly consistent with those in the field experiment. During the sampling period, intercropping reduced the concentration of As in the rhizosphere soil solution of L. cylindrica by 3.1-23.77%, while it increased the concentration of As in the rhizosphere soil solution of S. cassiae by 13.30-59.40%. The changes in pH and redox potential were also closely related to the content of water-soluble As in the rhizosphere environment, which affects the absorption of As by plants. In general, the L. cylindrica-S. cassiae intercropping system is a planting mode that can effectively treat soil that is moderately contaminated with As and remove it from the soil to an extent.
ABSTRACT
A batch experiment was conducted to examine the behavior of nitrate, organic ligands, and phosphate in the co-presence of biochar and three common low-molecular-weight organic acids (LMWOAs). The results show that citrate, oxalate, and malate ions competed with nitrate ion for the available adsorption sites on the biochar surfaces. The removal rate of LMWOA ligands by the biochar via adsorption grew with increasing solution pH. The adsorbed divalent organic ligands created negatively charged sites to allow binding of cationic metal nitrate complexes. A higher degree of biochar surface protonation does not necessarily enhance nitrate adsorption. More acidic conditions formed under a higher dose of LMWOAs tended to make organic ligands predominantly in monovalent forms and failed to create negatively charged sites to bind cationic metal nitrate complexes. This could adversely affect nitrate removal efficiency in the investigated systems. LMWOAs caused significant release of phosphate from the biochar. The phosphate in the malic acid treatment tended to decrease over time, while the opposite was observed in the citric- and oxalic-acid treatments. This was caused by re-immobilization of phosphate in the former due to the marked increase in solution pH over time.
Subject(s)
Nitrates , Phosphates , Adsorption , Charcoal/chemistry , Citric Acid/chemistry , Malates/chemistry , Nitrates/chemistry , Organic Chemicals/chemistry , Oxalic Acid/chemistry , Phosphates/chemistryABSTRACT
Biochar is a soil conditioner for enhancing plant growth and reducing plants' uptake of heavy metals. However, the protonation of biochar surfaces in acid soils can weaken the capacity of biochar to reduce the phytoavailability of soil-borne heavy metals over time. The aim of this study was to test this hypothesis by performing a plant-growth experiment with five harvest cycles to examine the durability of rice-straw biochar for the remediation of an acidic-mine-water-contaminated soil. The application of the biochar significantly reduced the phytoavailability of the heavy metals and inhibited the plant uptake of cationic heavy metals but not anionic Cr. The beneficial effects of the biochar were weakened with the increasing number of harvest cycles caused by the gradual protonation of the biochar surfaces, which resulted in the desorption of the adsorbed heavy metals. The weakening capacity of the biochar to reduce the heavy-metal uptake by the vegetable plants was more evident for Cu, Zn, and Pb compared to Ni and Cd. The experimental results generally confirmed the hypothesis. It was also observed that the bioaccessible amount of various metals in the edible portion of the vegetable was also reduced as a result of the biochar application.