Tissue distribution and correlations of heavy metals in wild birds from Southern Turkey: an ecologically important region on the west Palearctic migration route.

Even if some are essential for biological functions, the accumulation of heavy metals above tolerable physiological limits is potentially toxic to also wild animals. The present study aimed to investigate concentrations of environmentally relevant heavy metals (As, Cd, Cu, Fe, Hg, Mn, Pb, and Zn) in feathers, muscle, heart, kidney, and liver tissues of wild birds (golden eagle [Aquila chrysaetos], sparrowhawk [Accipiter nisus], and white stork [Ciconia ciconia]) from Hatay province, southern Turkey. The metal concentrations of tissues were determined by a validated ICP-OES analysis method after microwave digestion. The concentration differences of metals in species/tissues and the correlations between essential/non-essential metals were determined by statistical analysis. According to the results, Fe (326.87±3.60 mg kg-1) had the highest, and Hg (0.09±0.00 mg kg-1) had the lowest mean concentration in all tissues. Compared to the literature; Cu, Hg, Pb, and Zn concentrations were lower; Cd, Fe, and Mn concentrations were higher. The correlations between As and all essentials; Cd and Cu, Fe; Hg and Cu, Fe, Zn; Pb and all essentials were significantly positive. In the conclusion, while essential Cu, Fe, and Zn are below the threshold value and do not pose a risk, Mn is close to the threshold value. Therefore, periodically monitoring the pollutant concentrations in bioindicators is a key necessity for the early determination of biomagnification trends and prevention of potential toxic stress on wildlife ecology.

Concentration of essential and non-essential elements and carcinogenic / non-carcinogenic health risk assessment of commercial bee pollens from Turkey.

BACKGROUND: Bee pollen, known as a natural super-food with valuable nutritional ingredients, is regarded as a good indicator of ecotoxic substances, such as potentially toxic elements (PTEs). Therefore, this study aims to examine the concentrations of selected PTEs (Al, As, Ba, Cd, Co, Cr, Cu, Fe, Hg, Mg, Mn, Mo, Ni, Pb, Se, Sn, Sr, V, Zn) in bee pollen purchased from online markets in Turkey and perform a health risk assessment to identify the potential risk to consumers. METHODS: The quantitative analyses were conducted by inductively coupled plasma optical emission spectrometry (ICP-OES). RESULTS: The mean values of essential PTEs in decreasing content order were Mg > Fe > Zn > Mn > Cu > Ni > Se > Cr > Mo >Co = V. Regarding the results of the study, daily consumption (40 g for adult or 20 g for children) of commercial bee pollen can recompense 20-35 % of daily Cu, Mn, Se requirements for children, adults, pregnant, and breastfeeding women. The decreasing content order of non-essential elements was Al > Sn > Sr > Ba > Pb > As. Cadmium and Hg concentrations were below the detection limits in all the samples. In terms of food and public health; detection of the PTEs concentrations is necessary to assess the quality and safety of bee pollen before consumption. According to the carcinogenic and non-carcinogenic risk assessments; commercial pollen consumption does not pose a health risk to either children or adults for the PTEs monitored in this study. CONCLUSION: We conclude that bee pollen is an ideal indicator for the monitoring of environmental pollution of PTEs and also a valuable source of essential elements. This study highlights the need to develop standards that regulate acceptable concentrations of PTEs.

Total aflatoxin, aflatoxin B1, ochratoxin A and fumonisin in dry dog food: A risk assessment for dog health.

The aim of this study was to measure total aflatoxin (AFT), aflatoxin B1 (AFB1), ochratoxin A (OCA) and fumonisin (FUM) concentrations in dry dog feed and to evaluate the risk to animal health posed by their increased levels. A total of 90 dry food samples, which were commercially available to the owner, were collected from different shops in Turkey. Some of the food samples were collected from open packages, from which the dry food was sold in smaller amounts. Using commercial Enzyme-Linked Immunosorbent Assay test kits, all samples were examined for AFT, AFB1, OCA, and FUM concentrations. High-performance liquid chromatography was used for confirmation of measured parameters in 30 samples. The ELISA tests found AFT, AFB1, OCA, and FM concentrations (ng g -1) as 1.66, 0.64, 2.14, and 87.06, respectively. In terms of risk assessment, consumption of the dry foods, which are contaminated by AFT, AFB1 and OCA due possibly to the fact that the dry foods are produced from inappropriate raw material or sold in open packages in smaller amounts, poses a significant health risk for dogs. As a result, it is necessary to monitor the mycotoxin load in dry dog food as the use of raw materials of poor quality and selling the feed in smaller amounts from open packages over an uncertain time period predispose the dry feed to the growth of mycotoxin, especially when the storage conditions are favorable.

Blood concentrations of potentially toxic trace elements (PTEs) and correlation with biochemical and hematological parameters in dogs from thrace region, Turkey.

Environmental exposure to potentially toxic trace elements (PTEs) leads to health problems in animals as well as in humans. Dogs can be used as bioindicators for health status of both environment, animals and humans. The study material consisted of a total of 140 dogs from Thrace region in Turkey. Essential (Co, Cr, Cu, Fe, Zn) and non-essential (As, Cd, Hg, Ni, Pb) PTEs concentrations of blood samples were determined by ICP-MS. In addition, hemogram (RBC, MCV, MCH, MCHC, HCT, WBC, HGB, PLT) and biochemical parameters (Glucose, total protein, albumin, globulin, total bilirubin, triglyceride, cholesterol, BUN, AST, ALT, ALP, GGT) levels were determined. The possible correlations between PTEs and blood parameters were investigated. The results were compared according to gender, age (<2, 2-5, >5 years), sampled location with hemogram and biochemistry data. Essential element concentrations (ppb) were sorted as Zn > Fe > Cu > Cr > Co, and non-essentials were As > Ni > Pb > Hg > Cd. There were statistically important negative or positive correlations between elements and hematological (except Fe and Cd), and biochemical parameters (except Ni) (p < 0.05, p < 0.01). It was determined that Pb concentrations (9.34-23.30 ng ml-1) were below the concentrations considered to be toxic in all locations, Cu concentrations (475.35-521.98 ng ml-1) were within the normal reference range, Zn (3229.65-4.265.00 ng ml-1) were higher than the reference values in all locations. Since the concentrations of elements and correlations between hematological, biochemical parameters as well as gender, age, and location in an area with heavy urban and industrial activity; indicate that the situation may be similar for other living things in the region, it constitutes a starting point for studies to be carried out in this direction.

Health Risk Assessment of Essential and Toxic Metals in Canned/Pouched Food on Kitten and Adult Cats: an Animal Health Risk Assessment Adaptation Assay.

The main aim of this study was to determine the levels of Al, Cd, Cr, Cu, Fe, Hg, Mn, Ni, Pb, and Zn in commercial canned and pouched cat foods (salmon, tuna, liver, fish, and other aquatic products) and assess the potential health risks to kitten (≤ 1 years old) and adult cats (≥ 1 years old) associated with the recommended average consumption rate of labels. The study was also aimed to adapt the health risk assessment method to animal health and to support clinical prevention and diagnosis. The detected levels of the metals were below the data from other studies, except the mean Fe in all and Pb levels in salmon and kitten foods. Target hazard quotient (THQ) and total target hazard quotient (TTHQ) values did not exceed 1. That means the studied metals do not pose a health risk for adult cats and kittens. Dietary Hg and Cd should also be considered in the differential diagnosis of cases with clinical or postmortem findings, especially regarding neurological, kidney, and liver tissues. In conclusion, although canned/pouched consumption does not pose a health risk with regard to metals, further studies of health risk assessment for other pollutants by this first adaptation method will be necessary.

Distribution and Ecotoxicological Risk Assessment of Heavy Metals in Streams of Amanos Mountains from Southern Turkey.

Assessing the potential ecological risks of chemical pollutants like heavy metals is a key tool of a sustainable environment. With this goal, ecotoxicological significant metal (Al, As, Cd, Cr, Cu, Fe, Hg, Ni, Pb, and Zn) levels of the water (n = 32) and sediment (n = 32) samples of streams [rural (8 points) and urban (8 points) sides] on Amanos Mountains were determined by inductively coupled plasma-optical emission spectrometry (ICP-OES) and ecotoxicological risk assessment was performed through the potential ecological risk index (RI). The study region with intense urban activities has also ecological importance with regards to wildlife. It is located on the migration route of birds, hosts loggerhead and green sea turtles, Mediterranean seals, and some terrestrial species like mountain gazelle and striped hyena. All calculated RI values were below the potential risk limits and the ecotoxicological risk was observed to be very low. Metal levels should be monitored periodically, and necessary measures should be taken before the reflection of the increase to be determined by the risk assessment on the ecosystem.

Levels of Metals and Organochlorine Pesticides in Kidney, Liver, and Muscle Tissues of Wild Boars (Sus scrofa) from Hatay Province, Eastern Mediterranean Region, Turkey.

Increases in the levels of environmental contaminants are reflected in wild animals, which are early indicators of pollution. Hatay is an ecologically important region with a high intensity of industrial and agricultural activities. This study aimed to investigate the contamination levels of metals and organochlorine pesticides associated with environmental pollution in tissues of wild boars from Turkey. The highest mean levels (mg kg-1) of metals were 0.05 for As, 0.51 for Cd, 6.30 for Cu, 0.07 for Hg, 0.54 for Ni, and 0.57 for Pb in kidney tissues and 0.22 for Cr, 353.38 for Fe, 2.86 for Mn, and 46.76 for Zn in liver tissues. The Cd and Pb levels exceeded the maximum residue limits (MRLs) in some tissues. Among the studied pesticides, only p,p'-DDE contamination was quantified, and the mean levels were 3.6, 0.1, and 0.5 µg kg-1 in liver, kidney, and muscle tissues respectively. In conclusion, Hatay Province requires monitoring in terms of environmentally important contaminants, mainly Cd, Pb, and DDT isomers.

Hepatoprotective Activity of Linalool in Rats Against Liver Injury Induced by Carbon Tetrachloride.

This study aimed to investigate and compare hepatoprotective activity of Coriandrum sativum (Cs) and it is major component linalool (Ln) against experimentally induced hepatotoxicity in rats. Essential oil of Cs was isolated by hydrodistillation method and chemical composition was determined by GS-MS analysis. 42 male Wistar Albino rats were divited into 7 groups each containing 6. The experimental groups were designed as: Normal control group, 1 ml/kg CCl4 administirated group, 25 mg/kg Silymarin and CCl4 administirated group, 100 and 200 mg/kg Cs and CCl4 administirated groups, 100 and 200 mg/kg Ln and CCl4 administered groups. The protective activities were determined according to the results of liver biomarkers (AST, ALT, ALP), antioxidant parameters (GSH, GPx, CAT), lipid peroxidation (MDA) and histopathological examination. Linalool percentage of Cs was 81.6%. The groups treated with linalool (100 and 200 mg/kg) (p < 0.01) and coriander (200 mg/kg) (p < 0.05) had significantly reduced AST (262-375) and ALT (101-290) levels (U/L) compared to the CCl4 (600-622) group. The levels (nmol/g protein) of MDA (11-12) were significantly lower (p < 0.01), the levels of GSH (11-12) and the activities of CAT (23-24) were significantly higher (p < 0.01) in linalool groups (100 and 200 mg/kg) compared to the CCl4 (18-5-10 respectively) group. These results were also supported by histopathological findings and indicate that Cs and Ln shows hepatoprotective activity against liver damage. In this regard, evaluation of activities of major components are needed to compare to medicinal plants in experimental diseases models.

Protective effects of conventional and colon-targeted lycopene and linalool on ulcerative colitis induced by acetic acid in rats.

OBJECTIVE: To compare the potential protective effects of conventional and colon-targeted lycopene (TLC) and linalool (TLN) on acetic acid (AA)-induced ulcerative colitis (UC) in rats. METHODS: Conventional and colon-targeted LC (10 mg/kg) and LN (200 mg/kg) were administered in vivo orally for 7 days and sulfasalazine (100 mg/kg) was also used as reference drug. Then, 4% AA was administered intrarectally to induce UC. Subsequently, the colon tissues were taken as samples for biochemical and histopathological analysis. RESULTS: Malondialdehyde (MDA), interleukin 1ß (IL-1ß), IL-6, cyclooxygenase-2 (COX-2) and nuclear factor kappa B (NF-κB) levels were decreased (p < 0.05) in the targeted groups compared to the AA group, whereas nuclear factor-erythroid 2-related factor 2 (Nrf-2) level was increased (p < 0.05). Tumor necrosis factor α (TNF-α) level was also decreased (p < 0.05) and catalase activity (CAT) was increased (p < 0.05) in the TLC group compared to the AA group. IL-1ß and IL-6 levels were lower in the TLC group compared to the conventional LC and sulfasalazine groups (p < 0.05). COX-2 and NF-κB levels were lower, while the Nrf-2 level was higher in the targeted groups compared to the conventional groups (p < 0.05). Furthermore, COX-2 level was lower and Nrf-2 level was higher in the targeted groups compared to the sulfasalazine group (p < 0.05). CONCLUSION: As expected, sulfasalazine was effective on all parameters analyzed, but the colon-targeted pretreatments were more effective from sulfasalazine on some parameters. Therefore, colon-targeted plant-derived therapies might be alternative approaches to provide protection against UC, which deserves to be investigated further.

Characterization of extended spectrum ß-lactamase (ESBL)-producing Escherichia coli in Asi (Orontes) River in Turkey.

In this study, the presence of extended spectrum ß-lactamase (ESBL)-producing Escherichia coli in aquatic environments (the Orontes River and an urban wastewater) was investigated. Fifty-four E. coli strains resistant to cefotaxime were isolated from the river waters and nearby waste water treatment plant and screened for ESBL gene variants, different classes of integrons and sulfonamide resistance genes. The ESBL-producing E. coli strains were further characterized by PhP-typing system, phylogenetic grouping and antimicrobial susceptibility testing. Of the 54 ESBL-producing strains, 14 (25.9%) belonged to four common PhP types and the remaining were of single types. CTX-M type ESBL genes were identified in 68% of the isolates. The most predominant specific CTX-M subtype identified was blaCTX-M-15 (n = 36), followed by blaCTX-M-1 (n = 1). None of the isolates were SHV and OXA positive. Most of the ESBL positive isolates (n = 37; 68.5%) were harboring sul gene. This study indicates a widespread distribution of CTX-M-15 producing E. coli strains in the surface waters in part of Turkey, suggesting an aquatic reservoir for ESBL genes.

Heavy metal distribution in blood, liver and kidneys of Loggerhead (Caretta caretta) and Green (Chelonia mydas) sea turtles from the Northeast Mediterranean Sea.

The aim of the present study was to determine the concentrations of the most investigated environmentally relevant heavy metals in two highly endangered sea turtle species (Caretta caretta and Chelonia mydas) from the important nesting area on the Northeast Mediterranean Sea. The highest mean concentration was of Fe, while Hg and Pb were lowest. All tissue concentrations of Al, As, Fe and Mn were significantly different between the species. In particular, As, Cd, Cu, Mn, Ni, Se, Zn concentrations were lower in Caretta caretta and Cd, Hg, Mn, Zn concentrations were lower in Chelonia mydas than those reported in other parts of the world. Compared to studies conductud in other parts of the Mediterranean, Cd was lower.

Characterisation of Phenotypic and Genotypic Antibiotic Resistance Profile of Enterococci from Cheeses in Turkey.

The aim of this study was to determine the prevalence of enterococci in cheese samples and to characterize their antimicrobial resistance profiles as well as the associated resistance genes. A total of 139 enterococci were isolated from 99 cheese samples, the isolates were identified as E. faecalis (61.2%), E. faecium (15.1%), E. gallinarum (12.9%), E. durans (5.0%), E. casseliflavis (2.9%) and E. avium (2.9%). The most frequent antimicrobial resistance observed in enterococci isolates was to lincomycin (88.5%), followed by kanamycin (84.2%), gentamycin (low level, 51.1%), rifampin (46.8%) and tetracycline (33.8%). Among the isolates, the frequencies of high level gentamycin and streptomycin resistant enterococci strains were 2.2% and 5.8%, respectively. Apart from the mentioned antibiotics, low levels of resistance to ciprofloxacin, erythromycin and chloramphenicol were found. Moreover no resistance was observed against penicillin and ampicillin. The antimicrobial resistance genes including tetM, tetL, ermB, cat, aph(3')-IIIa, ant(6)-Ia and aac(6')-Ieaph(2")-Ia were found in enterococci from Turkish cheese samples. In the current study, we provided data for antibiotic resistance and the occurrence of resistance genes among enterococci. Regulatory and quality control programs for milk and other dairy products from farms to retail outlets has to be established and strengthened to monitor trends in antimicrobial resistance among emerging food borne pathogens in Turkey.

A risk assessment of heavy metal concentrations in fish and an invertebrate from the Gulf of Antalya.

This study was conducted to determine the levels of Cd, Cu, Zn and Pb in the Red mullet (Mullus barbatus), Grey mullet (Mugil cephalus) and Green tiger prawn (Panaeus semisulcatus) species caught in the Gulf of Antalya and to assess the health risks associated with their consumption. The average values (mg/kg d.w.) were calculated to be Pb (0.29 ± 0.14), Cd (0.02 ± 0.03), Zn (5.64 ± 1.58) and Cu (1.64 ± 1.02) in M. barbatus; Pb (0.22 ± 0.12), Cd (0.02 ± 0.01), Zn (7.66 ± 2.29) and Cu (1.33 ± 0.82) in M. cephalus; Pb (0.25 ± 0.16), Cd (0.04 ± 0.05), Zn (13.33 ± 3.34) and Cu (5.15 ± 2.09) in P. semisulcatus. The detected levels of the studied metals were below the legal and daily intake limits according to national and international standards, although the mean Pb level in M. barbatus was close to the legal limit (0.30 mg/kg). Target hazard quotient values were also all below 1, meaning that there is no health risk.

Concentrations of essential and non-essential toxic trace elements in wild boar (Sus Scrofa L., 1758) tissues from southern Turkey.

Wild animals, including wild boars, are suitable for use as bioindicators. The aim of this study was to evaluate the concentrations of selected essential (Co, Cu, Fe, Se, Zn) and non-essential (As, Cd, Pb) potentially toxic trace elements in various tissues (hair and hoof) of wild boars hunted in the vicinity of Antalya province in Turkey, in relation to hunting seasons. Concentrations in mg/kg on dry weight basis were determined as 0.37 ± 0.27 mg/kg for As, 0.05 ± 0.04 mg/kg for Cd, 0.24 ± 0.33 mg/kg for Co, 4.84 ± 2.48 mg/kg for Cu, 289.94 ± 165.26 mg/kg for Fe, 8.71 ± 15.68 mg/kg for Pb, 0.24 ± 0.10 mg/kg for Se, and 28.99 ± 21.41 mg/kg for Zn in the hair samples; and as 0.11 ± 0.04 mg/kg for As, 0.01 ± 0.04 mg/kg for Cd, 0.01 ± 0.01 mg/kg for Co, 1.03 ± 0.25 mg/kg for Cu, 56.88 ± 18.68 mg/kg for Fe, 0.30 ± 0.18 mg/kg for Pb, 0.11 ± 0.05 mg/kg for Se, and 17.91 ± 10.98 mg/kg for Zn in the hoof samples.