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1.
Theriogenology ; 148: 140-148, 2020 May.
Article in English | MEDLINE | ID: mdl-32171973

ABSTRACT

The molecule Dimethyl sulfoxide is widely used as drug solvent. However, its antioxidant property was poorly explored. In this study, we evaluated the effect of DMSO supplementation during oocyte in vitro maturation (IVM) on embryo development and quality. Bovine oocytes were matured with different DMSO concentrations (0, 0.1, 0.25, 0.5, 0.75, 1 and 10% v:v) followed by in vitro fertilization. Subsequently, quality indicators such as gene expression of SOX2, OCT4, CDX2, SOD1, oocyte and embryo redox status and DNA damage were evaluated. Polar body extrusion and blastocyst rates increased with 0.5% v:v DMSO. Moreover, first polar body extrusion and blastocyst rates did not increase with 1%, and 10% of DMSO reduced polar body extrusion and did not produce blastocyst. Optimal concentration of DMSO for the use on the maturation was estimated at around 0.45% v:v. Supplementation with 0.5% v:v DMSO has not affected mRNA abundance of genes key in blastocyst, however 0.75% increased gene expression of OCT4 and SOX2. Oocytes matured with 0.5% v:v DMSO and blastocyst from DMSO group showed reduced lipid peroxidation respect control. Total Glutathione concentrations increased in blastocyst stage in DMSO group. DMSO increased the total cell number of blastocysts but not TUNEL positive cells. In conclusion, our results suggest that low DMSO concentrations used during bovine oocytes in vitro maturation increases the maturation, as well as the blastocyst rate and its quality, without demonstrating deleterious effect on embryo cells.


Subject(s)
Blastocyst/physiology , Cattle , Dimethyl Sulfoxide/pharmacology , In Vitro Oocyte Maturation Techniques/veterinary , Oocytes/drug effects , Animals , CDX2 Transcription Factor/genetics , CDX2 Transcription Factor/metabolism , Culture Media , Dimethyl Sulfoxide/administration & dosage , Dose-Response Relationship, Drug , Embryo Culture Techniques/veterinary , Fertilization in Vitro/veterinary , Gene Expression Regulation, Developmental/drug effects , Glutathione/metabolism , Lipid Peroxidation , Octamer Transcription Factors/genetics , Octamer Transcription Factors/metabolism , SOXB1 Transcription Factors/genetics , SOXB1 Transcription Factors/metabolism , Superoxide Dismutase/metabolism , Superoxide Dismutase-1/genetics , Superoxide Dismutase-1/metabolism
2.
Reprod Fertil Dev ; 31(12): 1805-1811, 2019 Jan.
Article in English | MEDLINE | ID: mdl-31747534

ABSTRACT

Assisted reproduction techniques (ARTs) have become widespread in the equine breeding industry. In particular, the combination of oocyte recovery from live mares followed by IVM and intracytoplasmic sperm injection (ICSI) has increased markedly among the ARTs used with valuable or low-fertility animals. There is currently no consensus among research groups regarding the optimal oocyte maturation period to produce high-quality embryos. In this study, we report the maturation dynamics of equine oocytes at different time points, from 20 to 40h (Experiment 1). In addition, in Experiment 2, equine ICSI blastocysts were produced from oocytes that exhibited early (up to 24h) or late (28-30h) extrusion of the first polar body (PB). Blastocyst rates and diameter were recorded and embryo quality was assessed by analysing the number of apoptotic cells and Yes-associated protein 1 (YAP1) expression. By 20h of IVM, 42% of oocytes were mature, and the remaining oocytes matured within the next 17h of IVM. Although no differences were found in cell apoptosis or the number of YAP1-positive cells between groups exhibiting early and late PB extrusion, embryos from the early group (Group I) exhibited an improved total cell number and blastocyst rate compared to embryos from the late group (Group II) (18.60% vs 10.17% respectively).


Subject(s)
Blastocyst/physiology , Embryonic Development/physiology , Horses , Polar Bodies/physiology , Sperm Injections, Intracytoplasmic , Animals , Blastocyst/cytology , Blastocyst/ultrastructure , Cells, Cultured , Embryo Culture Techniques/veterinary , Embryo, Mammalian , Female , Horses/embryology , In Vitro Oocyte Maturation Techniques/veterinary , Male , Oocyte Retrieval/methods , Oocyte Retrieval/veterinary , Oogenesis/physiology , Sperm Injections, Intracytoplasmic/methods , Sperm Injections, Intracytoplasmic/veterinary , Time Factors
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