Effect of explosive versus slow contractions and exercise intensity on energy expenditure.

OBJECTIVE: The primary purpose of this study was to compare the effects of explosive versus slow contractions on the rate of energy expenditure during and after resistance exercise. METHODS: Nine men (20 +/- 2.5 yr) performed three exercise protocols using a plate-loaded squat machine, and a no-exercise (CONTROL) session in a randomly assigned, counterbalanced order. Subjects performed squats using either two second (SLOW) or explosive concentric contractions (EXPL), but identical repetitions (8), sets (4), and loads (60% 1RM). A secondary objective was to compare high- versus moderate-intensity exercise. Thus, a third protocol was performed that also used explosive contractions, with heavier loads (80% 1RM) and six sets of four reps (HEAVYEXPL). Eccentric reps (2 s), work (reps x sets x load), range of motion, and rest intervals between sets (90 s) were identical among all three protocols. Expired air was collected continuously for 20 min before, during, and 1 h after exercise and for about 1.5 h during CONTROL. Blood samples (25 microL) were collected before, immediately after, and 15, 30, 45, and 60 min after each protocol, and these samples were analyzed for blood lactate (mM). RESULTS: Average rates of energy expenditure (kcal.min) were significantly greater (P 6.43 +/- 1.64 and 6.25 +/- 1.55, respectively) and after (2.54 +/- 1.44 > 2.38 +/- 1.31 and 2.21 +/- 1.08, respectively) EXPL compared with SLOW and HEAVYEXPL, despite significantly (P

Determination of alachlor and its metabolites in rat plasma and urine by liquid chromatography-electrospray ionization mass spectrometry.

A method based on liquid chromatography (LC) in combination with mass spectrometry (MS) for the analysis of alachlor (ALA) and its metabolites, 2-chloro-N-[2,6-diethylphenyl]acetamide (CDEPA) and 2,6-diethylaniline (DEA), in rat plasma and urine has been developed. 13C-labeled ALA was used as the internal standard for quantitation. The analyte in plasma or urine was isolated using a Waters Oasis HLB extraction plate. The mass spectrometer was operated in the ESI MS-SIM mode with a programming procedure. The retention times for ALA, CDEPA and DEA were 1.84, 3.11 and 4.12 min, respectively. The limits of quantification (LOQ) for ALA, CDEPA and DEA were 2.3, 0.8 and 0.8 ng per injection, respectively. The linear fit of analyte to mass response had an R2 of 0.99. Reproducibility of the sample handling and LC-MS analysis had a RSD of < or = 10%. The average recoveries for these analytes in rat plasma were better than 90%. Similar results were obtained with rat urine.